Entry
Name
melilotate 3-monooxygenase;
2-hydroxyphenylpropionate hydroxylase;
melilotate hydroxylase;
2-hydroxyphenylpropionic hydroxylase;
melilotic hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-(2-hydroxyphenyl)propanoate,NADH:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
3-(2-hydroxyphenyl)propanoate + NADH + H+ + O2 = 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O [RN:
R03369 ]
Reaction(KEGG)
Substrate
Product
Comment
A flavoprotein (FAD).
History
EC 1.14.13.4 created 1972
Pathway
ec01120 Microbial metabolism in diverse environments
Reference
Authors
Levy CC.
Title
Melilotate hydroxylase. Purification of the enzyme and the nature of the prosthetic group.
Journal
J Biol Chem 242:747-53 (1967)
Reference
Authors
Levy CC, Frost P.
Title
The metabolism of coumarin by a microorganism. V. Melilotate hydroxylase.
Journal
J Biol Chem 241:997-1003 (1966)
Reference
Authors
Strickland S, Massey V.
Title
The purification and properties of the flavoprotein melilotate hydroxylase.
Journal
J Biol Chem 248:2944-52 (1973)
Reference
Authors
Strickland S, Massey V.
Title
The mechanism of action of the flavoprotein melilotate hydroxylase.
Journal
J Biol Chem 248:2953-62 (1973)
Other DBs
ExPASy - ENZYME nomenclature database: 1.14.13.4