KEGG ENZYME: 1.14.19.6

ENZYME: 1.14.19.6

Entry

EC 1.14.19.6                Enzyme

Name

acyl-CoA (9+3)-desaturase;
oleoyl-CoA 12-desaturase;
Delta12 fatty acid desaturase;
Delta12(omega6)-desaturase;
oleoyl-CoA Delta12 desaturase;
Delta12 desaturase;
Delta12-desaturase;
Delta12-fatty-acid desaturase;
acyl-CoA,hydrogen donor:oxygen Delta12-oxidoreductase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water

Sysname

acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (12,13 cis-dehydrogenating)

Reaction(IUBMB)

(1) oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11043];
(2) palmitoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11044]

Reaction(KEGG)

R11043 R11044

Substrate

oleoyl-CoA [CPD:C00510];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
palmitoleoyl-CoA [CPD:C21072]

Product

linoleoyl-CoA [CPD:C02050];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA [CPD:C21000]

Comment

This microsomal enzyme introduces a cis double bond at position 12 of fatty-acyl-CoAs that contain a cis double bond at position 9. When acting on 19:1Delta10 fatty acyl-CoA the enzyme from the pathogenic protozoan Trypanosoma brucei introduces the new double bond at position 13, indicating that the new double bond is introduced three carbons from the existing cis double bond, towards the methyl-end of the fatty acid. Requires cytochrome b5 as the electron donor [4].

History

EC 1.14.19.6 created 2008, modified 2015

Pathway

ec01040

Biosynthesis of unsaturated fatty acids

Orthology

K10256

omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)

K22993

bifunctional Delta-12/omega-3 fatty acid desaturase

Genes

AJM:

119047578

BCOO:

119067137 119073867 119077976 119084236 119085821

BTAB:

109031516 109034286 109037327 109038609 109038610 109038611 109043360 109043386 109043387 109043419 109043487 109043488 109043505

SGRE:

126324549

PVUL:

126811615

GAE:	121374992 121375004

HRF:

124116169

HRJ:	124268532 124268533

OSN:

115209645

NVE:

5512103

EPA:

110243700

ATEN:

116303763

ADF:

107344154

AMIL:

114948134 114973367

PDAM:

113667909 113682750

SPIS:

111329485 111342409

ATH:	AT3G12120(FAD2)

ALY:

9318834

CRB:

17890721

CSAT:

104745425 104764975 104776214

EUS:	EUTSA_v10020910mg

BRP:

103870276

BNA:	106417584 106452409 106452523

BOE:

106295108

RSZ:	108862913 130497099 130504985

THJ:	104799296 104826770

CPAP:

110811677

CIT:	102611163 102627239

CIC:	CICLE_v10011949mg CICLE_v10015614mg

PVY:	116125738 116136611

MINC:

123198158 123214921 123225237 123228010

TCC:	18589688 18599101

GRA:	105761671 105783314 105785452 105785453

GHI:	107901973 107921152 107921339 107924178 107934594 107941903 107945411 121212649

GAB:	108450424 108473778 108480547 108482302

HSYR:

120153918 120158105 120183510 120183934

DZI:	111295947 111304019 111311572

EGR:	104433804 104445836

GMX:	100780912(FAD2-2D) 100805040(FAD2-2B) 100805777(FAD2-1B) 100809290(FAD2-2A) 102662570 547814(FAD2-1A) 547815(FAD2-2) 732617(FAD2-3)

GSJ:	114367074 114370405 114400352 114401640 114406779

PVU:	PHAVU_001G142100g PHAVU_007G0234001 PHAVU_007G259500g

VRA:	106757695 106770720 106771256

VAR:	108323476 108325188 108325364

VUN:	114174864 114189943 114190784

VUM:	124820456 124827773 124827774 124830459

CCAJ:

109794836 109795239 109796052 109796649

APRC:

113861993 113872359 113873750

MTR:	11444765 25483518 25485564

TPRA:

123888747 123898279 123899802

CAM:	101489419 101507051 101513688

PSAT:

127098513 127126234

VVO:	131621445 131621556 131636358

LJA:	Lj1g3v4317650.1(Lj1g3v4317650.1) Lj5g3v2237610.1(Lj5g3v2237610.1) Lj5g3v2237610.2(Lj5g3v2237610.2)

ADU:	107464693 107467337 107492893

AIP:	107616829 107619143 107645414

AHF:	112696114 112710390 112712140 112755017 112776164 112779709

LANG:

109328907 109331342 109345961 109346314 109347587

PCIN:

129301231 129303850 129319588

QSA:	O6P43_004880 O6P43_005841 O6P43_008751 O6P43_009477

FVE:	101290788 101309231

RCN:	112194753 112201466

PPER:

18769718 18774949

PMUM:

103339420 103341921

PAVI:

110763023 110770740

PDUL:

117633799

MDM:	103427045 103443127 103454260

MSYL:

126592667 126599950 126616101

PXB:	103958977 103963937 103964864

ZJU:

107432351

MNT:	21386779 21391283 21401389

CSAV:

115701196 115718446 115719000 115719251 115719329

CSV:

101222623

CMO:

103486770

BHJ:	120088910 120090365

MCHA:

111024845

CMAX:

111470178 111481665 111484610 111490747

CMOS:

111441899 111448352 111448353 111459302

CPEP:

111777569 111777619 111786454 111786608 111789928 111795654 111800320

RCU:

8267086

JCU:	105646514 105650891

HBR:	110635031 110638358 110650724

MESC:

110621646 110621802 110622513

POP:	18094295 7455763 7479530 7483979

PEU:	105111221 105131774 105134978 105134979 105134982

PALZ:

118043755 118049781 118049805 118049806 118049817 118052395

JRE:	109001694 109011954 109021930

CILL:

122276083 122278215 122292068

CAVE:

132164512 132164706 132164733 132191453

QSU:	111998386 112012330 112015494 112015748

QLO:	115952674 115977653

TWL:	119995107 119996264 119998754 120017269

VVI:	100252129 100258789

VRI:	117915771 117920052

SLY:	101248657 101250481 101255474 101259966 109119181 112941338 544107(cevi19)

SPEN:

107003514 107005223 107005224 107005731 107005782 107005832 107006602 107015240 107017128

SOT:	102579196 102579518 102579844 102580407 102581806 102584924 102585247 102593159 102596973 102597622 102597941 102598049 102598267 102598608 102600240

SSTN:

125856711 125858454 125862308 125862309 125866588 125866589 125866590 125866591 125866592 125866605 125872824

SDUL:

129870260 129871090 129871655 129887782 129888911 129891120 129891129 129891137 129894236 129902073 129902272 129902336 129902412 129902486 129902550

CANN:

107843168 107849596 107849612 107851251 107851421 107851434 107851473 107851685 107851744 107873633 124894986

LBB:	132602713 132602715 132602716 132602717 132603721 132603722 132603733 132603735 132626002 132635573

NTA:	107773163 107797104 107814090 107823061(FAD2) 107829678 107831844

NSY:	104226786 104230317 104246180

NTO:	104089719 104090741 104098987 104106672 104121261

NAU:	109215055 109215819 109216417 109216418 109218761 109235687

INI:	109150720 109152414 109182042

ITR:	116007185 116007582 116007943 116024476 116029392

SIND:

105159686 105163532(FAD2)

OEU:	111365935 111371390 111390764 111393798 111399693

EGT:	105951255 105955229

SSPL:

121747569 121751845 121794328 121798703

SMIL:

131000922

SHIS:

125188161 125205310

APAN:

127258679 127258874 127263751

HAN:	110865031 110865032 110865236 110865239 110865277 110865281 110865284 110865285 110867681 110868684 110879372 110894444 110894736 110897314 110897316 110897318 110897319 110897568 110904312 110938218 110938220 110938221 110939886 110939887 110939961 110939971

ECAD:

122578445 122578764 122579373 122582919 122590523 122591112 122591269 122591356 122591604 122606452 122607353 122608062 122608674 122611246

LSV:	111879673 111882294 111884484 111885018 111889077 111889078 111889080 111889206 111890254 111896570 111896575 111896578 111896579 111896580 111896581 111896582 111897874 111899826 111900952 111900953 111904205 111908893 111909558 111915490 111918622

CCAV:

112505457 112505482 112505726 112506618 112506654 112506812 112506837 112506966 112506967 112506968 112512117 112513526 112521107 112521212 112521380 112521518 112521640 112521907 112521908 112521909 112521958 112525343 112526112 112527377 112527536 112527987 112528359 112528364

DCR:

108193493 108194523 108197230 108197256 108197419 108198376 108198633 108198634 108198635 108198637 108198638 108199375 108202677 108204656 108204690 108207522 108210933 108211018 108212237 108216232 108217089 108217440 108218481 108218482 108218742 108218743 108219326 108220169 108220600 108220695 108221065 108221821 108221989 108222165 108222186 108223766 108223875 108224006 108225347 108227781

CSIN:

114267865 114282301

RVL:	131315019 131315020 131324332 131324333

AEW:	130781454 130785560

BVG:	104889198 104889897

SOE:	110785100 110800912

CQI:	110689244 110695943 110734516 110736134

ATRI:

130796765 130820812 130825871

MOF:	131148005 131165865 131167705 131167706 131167708

NNU:	104597556 104603541 104611778

MING:

122061917 122087967

TSS:	122642840 122642959

PSOM:

113311026 113322906

OSA:	4330523 4343026 4343027

DOSA:

Os02t0716500-01(Os02g0716500) Os07t0416700-00(Os07g0416700) Os07t0416900-01(Os07g0416900) Os07t0417200-01(Os07g0417200)

OBR:

102719739

OGL:	127762104 127780007 127780184

BDI:	100835723 100837786

ATS:	109756354 109768827 109774922 109774938 109781985

TDC:	119317653 119317654 119321176 119322537 119326524

TAES:

123128186 123130860 123130862 123134421 123134423 123138008 123145288 123145289 123145293 123145295

TUA:	125512392 125512434 125514359 125514360

LPER:

127306411 127311774 127311775

LRD:

124692997

SBI:	110435045 8060698 8066368

ZMA:	100191328 100273207 100282744 100381639 103631986 103640919 118475586

SITA:

101768137 101783555

SVS:	117835694 117853495

PVIR:

120650240 120657027 120677804 120701919

PHAI:

112886426 112890525 112896900

PDA:	103713931 103723899

EGU:	105050201 105061227

MUS:	103971019 103976995 103980416 103997712 103999878

ZOF:	121993200 121997168 122020013 122023662 122032659 122035107

DCT:	110101255 110108827

PEQ:	110023778 110033260

AOF:	109833613 109843629 109843630

MSIN:

131222383

NCOL:

116257778

ATR:

18439780

SMO:	SELMODRAFT_163663

PPP:	112278989 112291137

CRE:	CHLRE_17g711150v5

VCN:	VOLCADRAFT_66373

MNG:

MNEG_7806

CVR:	CHLNCDRAFT_48812

CCP:	CHC_T00008296001

AGO:	AGOS_AFR589C

ERC:

Ecym_2769

KLA:	KLLA0_F07095g

KMX:	KLMA_20392

LTH:	KLTH0D13640g

ZRO:	ZYRO0B07414g

TGB:	HG536_0E01410

TDL:	TDEL_0E01510(TDEL0E01510)

ZMK:	HG535_0F01710

PPA:	PAS_chr4_0052

DHA:	DEHA2G05346g

PIC:	PICST_72518(ODD1)

PGU:	PGUG_02928

SPAA:

SPAPADRAFT_63149

LEL:	PVL30_005361

CAL:	CAALFM_C601110WA(FAD2)

CTP:	CTRG_02975

COT:	CORT_0F04690

CDU:	CD36_61210

CTEN:

CANTEDRAFT_114308

YLI:	YALI0B10153g

CLU:	CLUG_01582

CLUS:

A9F13_13g00209

CAUR:

CJI96_0004561

SLB:	AWJ20_1033(ODE1)

PKZ:	C5L36_0C09550 C5L36_0C09560

BNN:	FOA43_000373

BBRX:

BRETT_002981

OPA:	HPODL_03473

NCR:	NCU02209(ods) NCU09497

NTE:	NEUTE1DRAFT116751(NEUTE1DRAFT_116751) NEUTE1DRAFT86398(NEUTE1DRAFT_86398)

SMP:	SMAC_03650 SMAC_07882

PAN:	PODANSg2430

PBEL:

QC761_504330(FAH12) QC761_701450

PPSD:

QC762_504330(FAH12) QC762_701450

PPSP:

QC763_504330(FAH12) QC763_701450

PPSA:

QC764_504330(FAH12) QC764_701450

TTT:	THITE_76756

MTM:	MYCTH_50837 MYCTH_88639

CTHR:

CTHT_0000240 CTHT_0071750

MGR:	MGG_01985 MGG_08474

PPEI:

PpBr36_10589 PpBr36_10691

PGRI:

PgNI_00111 PgNI_05225

TMN:	UCRPA7_3220 UCRPA7_5047

SSCK:

SPSK_00128 SPSK_07620

FGR:	FGSG_05784 FGSG_07890

FPU:	FPSE_04515 FPSE_11043

FPOA:

FPOAC1_007204 FPOAC1_007328 FPOAC1_008748(FAH12) FPOAC1_011680 FPOAC1_013164 FPOAC1_013185 FPOAC1_013410 FPOAC1_013533 FPOAC1_013653 FPOAC1_013914 FPOAC1_013916

FVN:	FVRRES_09344 FVRRES_12734

FVR:	FVEG_05830 FVEG_12065

FOX:	FOXG_03793 FOXG_08835

NHE:	NECHADRAFT_102202 NECHADRAFT_66003

FFC:	NCS54_00197700 NCS54_00495900

FKR:	NCS57_00193500 NCS57_00533700

FMU:	J7337_004932(FAH12) J7337_007239

TRE:	TRIREDRAFT_45250 TRIREDRAFT_74228

TRR:	M419DRAFT_108308 M419DRAFT_26391

MAW:

MAC_06628

MAJ:

MAA_07889

PCHM:

VFPPC_10440

CMT:	CCM_06866 CCM_09560

AMUS:

LMH87_000016 LMH87_010184

PLJ:	VFPFJ_10241 VFPFJ_10448

PTKZ:

JDV02_003432(FAH12) JDV02_008161

VAL:	VDBG_10165

VDA:	VDAG_02971 VDAG_10469

CFJ:	CFIO01_04749 CFIO01_09629

CLUP:

CLUP02_11556 CLUP02_16261 CLUP02_17062

CHIG:

CH63R_11053 CH63R_12552

SAPO:

SAPIO_CDS0643 SAPIO_CDS7954

ELA:	UCREL1_10483 UCREL1_5610

PFY:	PFICI_02105 PFICI_05540 PFICI_11937

SSL:	SS1G_03438 SS1G_13683

BFU:	BCIN_01g05120 BCIN_01g10750 BCIN_02g06310 BCIN_13g00280

MBE:	MBM_04489 MBM_08304

PSCO:

LY89DRAFT_613354 LY89DRAFT_693845 LY89DRAFT_706947

GLZ:	GLAREA_04493 GLAREA_09934

ANI:	ANIA_01037 ANIA_07204

AFM:	AFUA_1G12530 AFUA_6G03050

ACT:	ACLA_022950 ACLA_097340

NFI:	NFIA_013020 NFIA_049390

AOR:	AO090001000224 AO090010000714

ANG:	An01g14970 An08g05160 An14g06980

AFV:	AFLA_008570 AFLA_014214

ALUC:

AKAW2_20019A AKAW2_30542S(FAH12) AKAW2_41307S

ACHE:

ACHE_30343S(FAH12)

APUU:

APUU_20896A APUU_50747S(FAH12)

PCS:	N7525_002119 N7525_002710 N7525_003929

PDP:	PDIP_27200 PDIP_61260

POU:	POX_c04082 POX_d06013

TMF:	EYB26_005492

TRG:	TRUGW13939_09539 TRUGW13939_10884

CIM:	CIMG_06871

CPW:	CPC735_032710

URE:	UREG_02885 UREG_03614

PBL:	PAAG_08655

PBN:	PADG_07259

ABE:

ARB_01637

TVE:

TRV_05152

AJE:	HCAG_01866

BGH:	BDBG_00166

PNO:	SNOG_01471

PTE:

PTT_13901

BZE:	COCCADRAFT_35692 COCCADRAFT_95357

BSC:	COCSADRAFT_255949 COCSADRAFT_263376

BOR:	COCMIDRAFT_80232 COCMIDRAFT_99684

AALT:

CC77DRAFT_261211 CC77DRAFT_674296 CC77DRAFT_946470

ARAB:

EKO05_0004585 EKO05_0008621

ZTR:	MYCGRDRAFT_101437

PFJ:	MYCFIDRAFT_72590 MYCFIDRAFT_86366

FFU:	CLAFUR5_03699 CLAFUR5_05071

CBET:

CB0940_02620 CB0940_11393

BCOM:

BAUCODRAFT_24063

NPA:	UCRNP2_9247 UCRNP2_969

TML:	GSTUM_00002898001

CNE:

CND02750

CNB:

CNBD3600

CNG:	CNAG_01150

CGI:	CGB_D5300C

CDEU:

CNBG_0718

TMS:	TREMEDRAFT_45585

TASA:

A1Q1_01239 A1Q1_06326

CCAC:

CcaHIS019_0112250(CcaverHIS019_0112250) CcaHIS019_0508550(CcaverHIS019_0508550)

TVS:	TRAVEDRAFT_147014 TRAVEDRAFT_28463 TRAVEDRAFT_38834

DSQ:	DICSQDRAFT_102286

PCO:	PHACADRAFT_265001

SHS:	STEHIDRAFT_104093

HIR:	HETIRDRAFT_440056

PSQ:	PUNSTDRAFT_141429

ADL:	AURDEDRAFT_115855

FME:	FOMMEDRAFT_128530

GTR:	GLOTRDRAFT_61689

RSX:	RhiXN_08914 RhiXN_08917

LBC:	LACBIDRAFT_292603(FAD12-5)

CCI:	CC1G_03449

ABP:	AGABI1DRAFT113988(AGABI1DRAFT_113988)

ABV:	AGABI2DRAFT193825(AGABI2DRAFT_193825)

PCUB:

JR316_0007061 JR316_0012985

MRR:	Moror_13992

E1B28_012456 E1B28_013509

SCM:	SCHCO_02615271(SCHCODRAFT_02615271)

CPUT:

CONPUDRAFT_56006

SLA:	SERLADRAFT_469833

WSE:	WALSEDRAFT_64045

WIC:	J056_000579

UMA:	UMAG_11270

PFP:	PFL1_00921

MGL:

MGL_1345

MRT:

MRET_4015

MSYM:

MSY001_0308

PGR:	PGTG_00088 PGTG_00121

PTRC:

PtA15_1A110 PtA15_1A111 PtA15_1A125

PSTR:

Pst134EA_001028 Pst134EA_001035 Pst134EA_001054 Pst134EA_004855 Pst134EA_004856

MLR:	MELLADRAFT_70376 MELLADRAFT_75337

SRE:	PTSG_08157

NGD:	NGA_0081700(DES)

TBR:	Tb927.2.3080

TBG:	TbgDal_II1450

TCR:

429257.20

LMA:	LMJF_33_3270

LIF:	LINJ_33_3420

LDO:	LDBPK_333420

LMI:	LMXM_32_3270

LBZ:	LBRM_33_3550

LPAN:

LPMP_101260 LPMP_333400

» show all

Reference

1 [PMID:2248971]

Authors

Borgeson CE, de Renobales M, Blomquist GJ.

Title

Characterization of the delta 12 desaturase in the American cockroach, Periplaneta americana: the nature of the substrate.

Journal

Biochim Biophys Acta 1047:135-40 (1990)
DOI:10.1016/0005-2760(90)90039-Z

Reference

2 [PMID:8900454]

Authors

Lomascolo A, Dubreucq E, Galzy P.

Title

Study of the delta 12-desaturase system of Lipomyces starkeyi.

Journal

Lipids 31:253-9 (1996)
DOI:10.1007/BF02529871

Reference

3 [PMID:9829122]

Authors

Tocher DR, Leaver MJ, Hodgson PA.

Title

Recent advances in the biochemistry and molecular biology of fatty acyl desaturases.

Journal

Prog Lipid Res 37:73-117 (1998)
DOI:10.1016/S0163-7827(98)00005-8

Reference

4 [PMID:15009186]

Authors

Petrini GA, Altabe SG, Uttaro AD

Title

Trypanosoma brucei oleate desaturase may use a cytochrome b5-like domain in another desaturase as an electron donor.

Journal

Eur J Biochem 271:1079-86 (2004)
DOI:10.1111/j.1432-1033.2004.04005.x

Sequence

[tbr:Tb927.2.3080]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.19.6

IUBMB Enzyme Nomenclature:

1.14.19.6

ExPASy - ENZYME nomenclature database:

1.14.19.6

BRENDA, the Enzyme Database:

1.14.19.6

DBGET integrated database retrieval system