| Entry |
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| Name |
ecdysone 20-monooxygenase;
alpha-ecdysone C-20 hydroxylase;
ecdysone 20-hydroxylase
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
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| Sysname |
Ecdysone,hydrogen-donor:oxygen oxidoreductase (20-hydroxylating)
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| Reaction(IUBMB) |
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O [RN: R02374]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
An enzyme from insect fat body or malpighian tubules involving a heme-thiolate protein (P-450). NADPH can act as ultimate hydrogen donor.
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| History |
EC 1.14.99.22 created 1978
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| Pathway |
| ec00981 | Insect hormone biosynthesis |
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| Orthology |
| K10723 | ecdysone 20-monooxygenase |
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| Genes |
| DSI: | Dsimw501_GD14493(Dsim_GD14493) |
| AALB: | 109413055 115261322 |
| BMOR: | 100127102(Cyp314a1) |
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| Reference |
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| Authors |
Johnson P, Rees HH. |
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| Title |
The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria. |
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| Journal |
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| Reference |
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| Authors |
Nigg HN, Svoboda JA, Thompson MJ, Dutky SR, Kaplanis JN, Robbins WE. |
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| Title |
Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.). |
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| Journal |
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| Reference |
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| Authors |
Smith SL, Bollenbacher WE, Cooper DY, Schleyer H, Wielgus JJ, Gilbert LI. |
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| Title |
Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase. |
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| Journal |
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| Other DBs |
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