KEGG ENZYME: 1.14.99.39

ENZYME: 1.14.99.39

Entry

EC 1.14.99.39               Enzyme

Name

ammonia monooxygenase;
AMO

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous

Sysname

ammonia,donor:oxygen oxidoreductase (hydroxylamine-producing)

Reaction(IUBMB)

NH3 + a reduced acceptor + O2 = NH2OH + an acceptor + H2O [RN:R09519]

Reaction(KEGG)

R09519 > R00148;
(other) R09156

Substrate

NH3 [CPD:C00014];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]

Product

NH2OH [CPD:C00192];
acceptor [CPD:C00028];
H2O [CPD:C00001]

Comment

The enzyme catalyses the first reaction in the pathway of ammonia oxidation to nitrite. It contains copper [1], iron [5] and possibly zinc [9]. The enzyme requires two electrons, which are derived indirectly from the quinone pool via a membrane-bound donor.

History

EC 1.14.99.39 created 2010

Pathway

ec00910

Nitrogen metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K10944

methane/ammonia monooxygenase subunit A

Genes

MCA:	MCA1797(pmoA) MCA2854(pmoA2)

METU:

GNH96_03570 GNH96_09690

MMEO:

OOT43_00490 OOT43_14055

MMT:	Metme_0304

MDN:	JT25_004545 JT25_012445

MDH:	AYM39_18580 AYM39_19770

MKO:	MKLM6_3758 MKLM6_3997

METL:

U737_00525 U737_22530

MPAD:

KEF85_05595 KEF85_15100

MELL:

IVG45_01190 IVG45_17415

MRP:	NM686_001765

MMOT:

QZJ86_01655(amoA)

MAH:	MEALZ_0515(pmoA)

MBUR:

EQU24_19305

MPSY:

CEK71_17435

MMAI:

sS8_1788 sS8_3977

MSZE:

MSZNOR_3294(pmoA) MSZNOR_4515(pmoA)

MMOB:

F6R98_01475 F6R98_16060

MEIN:

methR_P0112

MOZ:	MoryE10_29180 MoryE10_29210

MISZ:

MishRS11D_20920 MishRS11D_40480 MishRS11D_42080

MESL:

KKZ03_06640

MEIY:

MIN45_P0548 MIN45_P2284

MCAU:

MIT9_P0452 MIT9_P0923

NOC:

Noc_2502

NHL:

Nhal_0676

NWA:

Nwat_0632

NWR:	E3U44_00915

NTT:	TAO_1521(amoA)

NTG:	NSCAC_1278(amoA)

HPSE:

HPF_13160(pmoA1)

NEU:	NE0944(amoA1) NE2063(amoA2)

NET:	Neut_2077 Neut_2318

NIT:	NAL212_0798 NAL212_1387 NAL212_2605

NII:	Nit79A3_0472 Nit79A3_1080 Nit79A3_2885

NCO:	AAW31_01085 AAW31_05380

NUR:	ATY38_01310 ATY38_07255 ATY38_13755

NST:	Nstercoris_00328 Nstercoris_01426

NMU:	Nmul_A0799 Nmul_A2325 Nmul_A2765

NLC:	EBAPG3_006230 EBAPG3_014830 EBAPG3_15005

NIZ:	NNRS527_00820 NNRS527_02502 NNRS527_03102

AZR:	CJ010_10955

BAUT:

QA635_32490

MEDK:

QEV83_09615(amoA)

MSC:	BN69_0203(pmoA2) BN69_2827(pmoA) BN69_3534(pmoA)

MBRY:

B1812_00565 B1812_13465 B1812_14775 B1812_18680

MROS:

EHO51_01980 EHO51_08605

MHEY:

H2LOC_006300 H2LOC_011625 H2LOC_017530

MPAR:

F7D14_03105 F7D14_11665 F7D14_16695

MIWA:

SS37A_06920 SS37A_17860 SS37A_28170

MTW:	CQW49_01220 CQW49_10255

MECQ:

MSC49_20790 MSC49_22310 MSC49_34660

MDX:	BTO20_38255

MCB:	Mycch_5910

MRH:	MycrhN_3041

MIN:	Minf_1507(pmoA) Minf_1510(pmoA) Minf_1590(pmoA)

MKC:	kam1_1359(pmoA3) kam1_1509(pmoA2) kam1_1512(pmoA1) kam1_1972(pmoA4)

MFH:	MFUM_1605(pmoA3) MFUM_1792(pmoA1) MFUM_1795(pmoA2)

MCAO:

IT6_09400 IT6_09940 IT6_09955

MEAP:

MTHMO_0894(pmoA1) MTHMO_0897(pmoA2)

NIO:	NITINOP_1853(amoA)

NKF:	Nkreftii_003170

MOX:	DAMO_2450(pmoA)

NMR:

Nmar_1500

NIR:	NSED_08255

NKR:	NKOR_08170

NID:	NPIRD3C_2147(amoA)

NIN:	NADRNF5_0394(amoA)

NIW:	Nisw_06415

NCL:	C5F47_07870

NOX:	C5F49_07755

NUE:	C5F50_11085

NIP:	NsoK4_00795

NZT:	NZOSNM25_000290

NCT:

NMSP_0259

NIC:	DSQ20_08765

NGA:	Ngar_c25350(amoA)

NVN:	NVIE_027270(amoA)

NEV:

NTE_00961

TAA:	NMY3_03280

NFN:	NFRAN_0675(amoA)

NCV:	NCAV_0492(amoA)

CSY:	CENSYa_0402

NBV:

T478_0302

TAH:	SU86_008325

NIU:	DSQ19_08460

NCK:	QVH35_01005

NDV:	NDEV_1777(amoA)

» show all

Reference

1 [PMID:8458839]

Authors

Ensign SA, Hyman MR, Arp DJ

Title

In vitro activation of ammonia monooxygenase from Nitrosomonas europaea by copper.

Journal

J Bacteriol 175:1971-80 (1993)
DOI:10.1128/JB.175.7.1971-1980.1993

Reference

2 [PMID:8085841]

Authors

Hyman MR, Page CL, Arp DJ

Title

Oxidation of methyl fluoride and dimethyl ether by ammonia monooxygenase in Nitrosomonas europaea.

Journal

Appl Environ Microbiol 60:3033-5 (1994)
DOI:10.1128/AEM.60.8.3033-3035.1994

Reference

3 [PMID:7980540]

Authors

Bergmann DJ, Hooper AB

Title

Sequence of the gene, amoB, for the 43-kDa polypeptide of ammonia monoxygenase of Nitrosomonas europaea.

Journal

Biochem Biophys Res Commun 204:759-62 (1994)
DOI:10.1006/bbrc.1994.2524

Sequence

[neu:NE0944 NE0943]

Reference

4 [PMID:7590173]

Authors

Holmes AJ, Costello A, Lidstrom ME, Murrell JC

Title

Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related.

Journal

FEMS Microbiol Lett 132:203-8 (1995)
DOI:10.1111/j.1574-6968.1995.tb07834.x

Reference

5 [PMID:8941709]

Authors

Zahn JA, Arciero DM, Hooper AB, DiSpirito AA

Title

Evidence for an iron center in the ammonia monooxygenase from Nitrosomonas europaea.

Journal

FEBS Lett 397:35-8 (1996)
DOI:10.1016/S0014-5793(96)01116-7

Reference

6 [PMID:8654570]

Authors

Moir JW, Crossman LC, Spiro S, Richardson DJ

Title

The purification of ammonia monooxygenase from Paracoccus denitrificans.

Journal

FEBS Lett 387:71-4 (1996)
DOI:10.1016/0014-5793(96)00463-2

Reference

7 [PMID:11004450]

Authors

Whittaker M, Bergmann D, Arciero D, Hooper AB

Title

Electron transfer during the oxidation of ammonia by the chemolithotrophic bacterium Nitrosomonas europaea.

Journal

Biochim Biophys Acta 1459:346-55 (2000)
DOI:10.1016/S0005-2728(00)00171-7

Reference

8 [PMID:12209257]

Authors

Arp DJ, Sayavedra-Soto LA, Hommes NG.

Title

Molecular biology and biochemistry of ammonia oxidation by Nitrosomonas europaea.

Journal

Arch Microbiol 178:250-5 (2002)
DOI:10.1007/s00203-002-0452-0

Reference

9 [PMID:19453274]

Authors

Gilch S, Meyer O, Schmidt I

Title

A soluble form of ammonia monooxygenase in Nitrosomonas europaea.

Journal

Biol Chem 390:863-73 (2009)
DOI:10.1515/BC.2009.085

Other DBs

ExplorEnz - The Enzyme Database:

1.14.99.39

IUBMB Enzyme Nomenclature:

1.14.99.39

ExPASy - ENZYME nomenclature database:

1.14.99.39

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.99.39

BRENDA, the Enzyme Database:

1.14.99.39

DBGET integrated database retrieval system