KEGG ENZYME: 1.3.8.3

ENZYME: 1.3.8.3

Entry

EC 1.3.8.3                  Enzyme

Name

(R)-benzylsuccinyl-CoA dehydrogenase;
BbsG;
(R)-benzylsuccinyl-CoA:(acceptor) oxidoreductase

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With a flavin as acceptor

Sysname

(R)-benzylsuccinyl-CoA:electron transfer flavoprotein oxidoreductase

Reaction(IUBMB)

(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein = (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein

Reaction(KEGG)

(other) R05584

Substrate

(R)-2-benzylsuccinyl-CoA [CPD:C09817];
electron-transfer flavoprotein [CPD:C04253]

Product

(E)-2-benzylidenesuccinyl-CoA [CPD:C09818];
reduced electron-transfer flavoprotein [CPD:C04570]

Comment

Contains a tightly-bound FAD cofactor. Unlike other acyl-CoA dehydrogenases, this enzyme exhibits high substrate- and enantiomer specificity; it is highly specific for (R)-benzylsuccinyl-CoA and is inhibited by (S)-benzylsuccinyl-CoA. Forms the third step in the anaerobic toluene metabolic pathway in Thauera aromatica. Ferricenium ion is an effective artificial electron acceptor.

History

EC 1.3.8.3 created 2003 as EC 1.3.99.21, transferred 2012 to EC 1.3.8.3

Pathway

ec00623

Toluene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K07545

(R)-benzylsuccinyl-CoA dehydrogenase

Genes

BFZ:	BAU07_03785

BGM:	CAL15_20545

ADY:	HLG70_07275

BOZ:	DBV39_02120

VEI:

Veis_2517

EBA:	c2A310(bbsG)

APET:

ToN1_01540(bbsG)

AZI:	AzCIB_4519

AZD:	CDA09_07455 CDA09_21960

TCL:

Tchl_1391

TAK:	Tharo_3084

GME:	Gmet_1523(bbsG)

GEO:	Geob_2422(bbsG)

DTO:	TOL2_C01530(bbsG)

DMM:	dnm_089810(bhsG)

DGI:	Desgi_0647

CHRE:

IE160_04110

» show all

Reference

1 [PMID:10589736]

Authors

Leutwein C, Heider J.

Title

Anaerobic toluene-catabolic pathway in denitrifying Thauera aromatica: activation and beta-oxidation of the first intermediate, (R)-(+)-benzylsuccinate.

Journal

Microbiology 145 ( Pt 11):3265-71 (1999)
DOI:10.1099/00221287-145-11-3265

Reference

2 [PMID:12420174]

Authors

Leutwein C, Heider J

Title

(R)-Benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway.

Journal

Arch Microbiol 178:517-24 (2002)
DOI:10.1007/s00203-002-0484-5

Sequence

[ag:AAF89842]