KEGG ENZYME: 1.3.99.5

ENZYME: 1.3.99.5

Entry

EC 1.3.99.5                 Enzyme

Name

3-oxo-5alpha-steroid 4-dehydrogenase (acceptor);
steroid 5alpha-reductase;
3-oxosteroid Delta4-dehydrogenase;
3-oxo-5alpha-steroid Delta4-dehydrogenase;
steroid Delta4-5alpha-reductase;
Delta4-3-keto steroid 5alpha-reductase;
Delta4-3-oxo steroid reductase;
Delta4-3-ketosteroid5alpha-oxidoreductase;
Delta4-3-oxosteroid-5alpha-reductase;
3-keto-Delta4-steroid-5alpha-reductase;
5alpha-reductase;
testosterone 5alpha-reductase;
4-ene-3-ketosteroid-5alpha-oxidoreductase;
Delta4-5alpha-dehydrogenase;
3-oxo-5alpha-steroid:(acceptor) Delta4-oxidoreductase;
tesI (gene name)

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors

Sysname

3-oxo-5alpha-steroid:acceptor Delta4-oxidoreductase

Reaction(IUBMB)

a 3-oxo-5alpha-steroid + acceptor = a 3-oxo-Delta4-steroid + reduced acceptor [RN:R02643]

Reaction(KEGG)

R02643 > R01834 R04827

Substrate

3-oxo-5alpha-steroid [CPD:C02940];
acceptor [CPD:C00028]

Product

3-oxo-Delta4-steroid [CPD:C00619];
reduced acceptor [CPD:C00030]

Comment

A flavoprotein. This bacterial enzyme, characterized from Comamonas testosteroni, is involved in androsterone degradation. cf. EC 1.3.1.22, 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+).

History

EC 1.3.99.5 created 1965, modified 2012

Pathway

ec00984

Steroid degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K16051

3-oxo-5alpha-steroid 4-dehydrogenase

Genes

RGL:	CS053_01900

PRE:	PCA10_43670

PFUW:

KF707C_11860

PPUT:

L483_14100

PPUN:

PP4_27190

PPUU:

PputUW4_01680

PKC:

PKB_4321

PFZ:	AV641_10570

PLQ:	AA042_20375

PPSY:

AOC04_07870

PSET:

THL1_4409

PSIL:

PMA3_11575

PKE:	DLD99_14665

PUM:	HGP31_20980

PPSH:

G5J76_13525

PLAL:

FXN65_21135

PAZE:

KSS91_19195

PTAE:

NCTC10697_02064(ifcA_1)

PCAV:

D3880_17460

PIZ:	LAB08_R40770

PKM:	PZ739_11580

HARR:

HV822_17845

KAK:	Kalk_07950

CBER:

B5D82_11210

PHA:

PSHAa2146

PTN:	PTRA_a2599

PNG:	PNIG_a2794

SNAN:

I6N98_14785

HJA:	BST95_03540

HALC:

EY643_04225 EY643_18950

AFUS:

EYZ66_02430 EYZ66_13095

MSEC:

LN244_04835

MRZ:	KDW95_00830

ENW:	MJO57_21290

EMP:	EZMO1_1275(tesI)

SDF:	ACG33_03810

SOK:	D0B54_05270 D0B54_08595 D0B54_16855

SINI:

GT972_08465 GT972_09470

FCE:	JN531_005455

GPB:	HDN1F_19870(tesI)

RIN:	ACS15_4365

REH:	H16_B0641(h16_B0641)

CNC:	CNE_2c05860 CNE_BB1p04270(ifcA1) CNE_BB1p06940(fccA)

CUH:	BJN34_30935

REU:	Reut_A1659

CTI:	RALTA_B0490

CBW:	RR42_s2342

COX:	E0W60_08150

CUK:	KB879_20645

BUR:	Bcep18194_B1518

BCJ:

BCAM1577

BCEN:

DM39_3421

BCEO:

I35_5434

BCT:

GEM_4153

BCED:

DM42_6385

BCEP:

APZ15_31415

BPYR:

ABD05_28050

BCON:

NL30_04390

BDF:	WI26_20960

BTEI:

WS51_05515

BPSL:

WS57_06230

BMEC:

WJ16_26540

BSTL:

BBJ41_21090

BANN:

JFN94_25425

BARI:

NLX30_30430

BAEN:

L3V59_21485

BURK:

DM992_32830

BFN:

OI25_4613

PBRY:

NDK50_33000

PPAI:

E1956_26865 E1956_35685 E1956_37100

CABA:

SBC2_48770

CTT:	CtCNB1_1358

CTES:

O987_07765

CRJ:	QMY55_07045

DIH:	G7047_09730

DOE:	DENOEST_0485 DENOEST_3316 DENOEST_3670 DENOEST_3673

THU:	AC731_008850

TSV:	DSM104635_00611

NAR:	Saro_3488 Saro_3686 Saro_3721

NPP:	PP1Y_AT13465 PP1Y_Mpl3136 PP1Y_Mpl3259

NOT:	C7W88_20740

SWI:	Swit_1005 Swit_1860 Swit_3335 Swit_3339

SPHD:

HY78_08855 HY78_08875 HY78_16270 HY78_21490

RDI:	CMV14_19635

SPHM:

G432_01970 G432_02370 G432_07425

SSAN:

NX02_12695 NX02_12730

SPKC:

KC8_03545 KC8_05240 KC8_10970

SPHA:

D3Y57_03785 D3Y57_15195

SLAN:

GV829_01840

SPMI:

K663_02990

SPHB:

EP837_01509(tesI)

SPHG:

AZE99_00535

SFLA:

SPHFLASMR4Y_02380(ifcA)

PHZ:	CHX26_05335

NTD:	EGO55_03145 EGO55_05895 EGO55_13615 EGO55_13660

CAEN:

K5X80_05260 K5X80_13780

GLO:

Glov_1298

DSY:	DSY0764 DSY3803

DHD:

Dhaf_0773

DDH:	Desde_0646

EGD:	GS424_005060 GS424_006825

AHAT:

ADCFC_06820 ADCFC_14480

MTV:

RVBD_1817

MRA:

MRA_1829

MTF:	TBFG_11847

MTB:	TBMG_02177

MTK:	TBSG_02188

MTZ:	TBXG_002159

MTI:	MRGA423_11360

MTE:	CCDC5079_1674

MTUR:

CFBS_1909

MTL:	CCDC5180_1654

MTO:	MTCTRI2_1849

MTD:

UDA_1817

MTN:	ERDMAN_2007

MTJ:	J112_09705

MTUB:

MT7199_1843

MTUC:

J113_12610

MTUE:

J114_09700

MTX:	M943_09450

MTUH:

I917_12910

MTUL:

TBHG_01774

MTUT:

HKBT1_1906

MTUU:

HKBT2_1914

MTQ:	HKBS1_1910

MBO:	BQ2027_MB1848

MBB:

BCG_1852

MBT:

JTY_1836

MBM:	BCGMEX_1833

MBK:	K60_019030

MAF:

MAF_18390

MMIC:

RN08_2021

MCE:	MCAN_18331

MCQ:	BN44_40077

MCV:	BN43_30955

MCX:	BN42_30078

MCZ:	BN45_50080

MORY:

MO_001915

MPA:	MAP_1447c MAP_1530

MAO:	MAP4_2309 MAP4_2397

MAVI:

RC58_11475 RC58_11915

MAVU:

RE97_11485 RE97_11925

MAV:	MAV_2897 MAV_3022

MIT:

OCO_28580

MIA:

OCU_28450

MID:

MIP_03716

MYO:

OEM_27040

MCHI:

AN480_14940

MIR:	OCQ_25850 OCQ_27160

MMAL:

CKJ54_12985

MLP:	MLM_2334 MLM_2414

MMAN:

MMAN_10300

MSA:	Mycsm_03249 Mycsm_04160

MUL:	MUL_3063 MUL_4900

MMI:	MMAR_0205 MMAR_2694

MMAE:

MMARE11_01890 MMARE11_26150

MLI:	MULP_00179 MULP_02440

MPSE:

MPSD_27690 MPSD_53970

MSHO:

MSHO_12420 MSHO_40180

MMC:	Mmcs_0302 Mmcs_0316

MKM:	Mkms_0312 Mkms_0326

MJL:	Mjls_0293 Mjls_0307

MMM:

W7S_13850

MKN:	MKAN_15315

MYV:	G155_02520 G155_04245

MYE:	AB431_15170 AB431_15230 AB431_17075

MHAD:

B586_11860

MDX:	BTO20_15410

MSHG:

MSG_02470 MSG_02584

MFJ:	MFLOJ_06530 MFLOJ_07950 MFLOJ_21820

MSTO:

MSTO_07790 MSTO_09190 MSTO_25520

MSIM:

MSIM_53030

MSAK:

MSAS_06930

MKU:	I2456_01305 I2456_01760 I2456_13340

MLW:	MJO58_13010

MXE:	MYXE_06650 MYXE_25900

MNV:	MNVI_11580 MNVI_40650

MPAG:

C0J29_01190 C0J29_15540

MNM:	MNVM_19340 MNVM_40340 MNVM_41400

MGOR:

H0P51_01160 H0P51_14660 H0P51_17860

MCOO:

MCOO_26130

MBAI:

MB901379_00137 MB901379_02490(fccA)

MSEO:

MSEO_12700 MSEO_14010

MHEK:

JMUB5695_00574 JMUB5695_02538

MGAU:

MGALJ_22860

MLJ:	MLAC_17620 MLAC_19640

MBRD:

MBRA_21110 MBRA_39620

MSHJ:

MSHI_01900 MSHI_13780

MMAM:

K3U93_11790 K3U93_12395

MSPG:

F6B93_00910 F6B93_11130

MOT:	LTS72_10745 LTS72_24685

MPAA:

MKK62_02905

MKY:	IWGMT90018_02350

MWU:	PT015_15060

MSM:	MSMEG_2873

MSG:	MSMEI_2799 MSMEI_3570

MSB:	LJ00_14290 LJ00_18180

MSN:	LI99_14295 LI99_18185

MSH:	LI98_14300 LI98_18190

MVA:	Mvan_2890 Mvan_3165

MGI:	Mflv_3411 Mflv_4550 Mflv_4564

MSP:	Mspyr1_27430 Mspyr1_39500 Mspyr1_39640

MCB:	Mycch_1589 Mycch_2743

MNE:	D174_15875

MYN:	MyAD_15600

MFT:	XA26_04570 XA26_08060

MPHL:

MPHLCCUG_02346(ifcA) MPHLCCUG_02608

MVQ:	MYVA_1640 MYVA_2914 MYVA_5433

MLL:	B1R94_12245 B1R94_13935 B1R94_20090

MRH:	MycrhN_4258 MycrhN_4805

MTHN:

4412656_00946(ifcA_1) 4412656_02236(ifcA_2)

MHAS:

MHAS_01796(fccA) MHAS_02476

MDU:	MDUV_13340 MDUV_26670

MCHT:

MCHIJ_12690 MCHIJ_37430

MAUU:

NCTC10437_02738(ifcA_1) NCTC10437_03040(ifcA_2)

MMOR:

MMOR_26790 MMOR_32850

MFX:	MFAL_10920 MFAL_17780

MAIC:

MAIC_26780 MAIC_26920 MAIC_29760

MIJ:	MINS_21440 MINS_28360

MALV:

MALV_29240 MALV_32080

MTY:	MTOK_49090

MPSC:

MPSYJ_36770

MARZ:

MARA_10100

MGAD:

MGAD_01090 MGAD_07420

MHEV:

MHEL_50110 MHEL_50210 MHEL_53560

MSAR:

MSAR_38820 MSAR_41360

MANY:

MANY_09470 MANY_15450

MAUB:

MAUB_04580 MAUB_07440 MAUB_15650 MAUB_54780

MPOF:

MPOR_33230

MPHU:

MPHO_08050 MPHO_14380 MPHO_55420

MMUC:

C1S78_011090 C1S78_014220 C1S78_015715 C1S78_019255 C1S78_019905

MBOK:

MBOE_16350 MBOE_20100

MFG:	K6L26_27690

MFLV:

NCTC10271_02169(ifcA_1) NCTC10271_02186(ifcA_2)

MCEE:

MCEL_06110

MMON:

EWR22_01615 EWR22_01685

MGO:	AFA91_21605

MHOL:

K3U96_09630

MPAE:

K0O64_14650 K0O64_14695 K0O64_16250

MRF:	MJO55_13870

MDF:	K0O62_10315 K0O62_15920

MCRO:

MI149_15505 MI149_15555 MI149_17150

MGRO:

FZ046_19800

MKR:	MKOR_07390 MKOR_08240

MPAK:

MIU77_08035 MIU77_08480

MAB:

MAB_0623

MMV:	MYCMA_0323 MYCMA_1290

MABB:

MASS_0593 MASS_2307

MABL:

MMASJCM_0612 MMASJCM_2330

MCHE:

BB28_02985 BB28_11950

MIZ:	BAB75_03735 BAB75_12875

MSTE:

MSTE_00582 MSTE_02324

MSAO:

MYCSP_02685 MYCSP_11035

MSAL:

DSM43276_00541 DSM43276_02107

MJD:	JDM601_1914 JDM601_2019 JDM601_4251

MTER:

4434518_01865(ifcA) 4434518_01971

MMIN:

MMIN_35580 MMIN_36500

MHIB:

MHIB_10920 MHIB_13900 MHIB_14890

MHER:

K3U94_10960 K3U94_11545

MVM:	MJO54_11005 MJO54_11545

ASD:

AS9A_1017

NFA:	NFA_30830 NFA_4640 NFA_46590

NFR:	ERS450000_00982(ifcA_1) ERS450000_03773(ifcA_3) ERS450000_05269(ifcA_4)

NCY:	NOCYR_0491 NOCYR_3994 NOCYR_4393

NBR:	O3I_002615 O3I_020135 O3I_020920

NNO:	NONO_c03420

NSL:	BOX37_16630 BOX37_17180

NSR:	NS506_00426(tesI) NS506_06811

NTP:	CRH09_02420

NOZ:	DMB37_00255

NOD:	FOH10_02275 FOH10_22680 FOH10_29845

NAH:	F5544_02605

NAD:	NCTC11293_03870(ifcA_1) NCTC11293_04109(ifcA_2)

NWL:	NWFMUON74_03310

NIE:	KV110_02995 KV110_20565

NTC:	KHQ06_07725 KHQ06_14305 KHQ06_35220 KHQ06_36955

NHU:	H0264_02930 H0264_13240 H0264_34360 H0264_36175

NYA:	LTV02_19480 LTV02_29815 LTV02_37155 LTV02_38585

NGP:	LTT66_15370 LTT66_28710 LTT66_35130

NSPU:

IFM12276_01570 IFM12276_28600 IFM12276_30610 IFM12276_35580

NYU:	D7D52_03615 D7D52_09580 D7D52_32640

RHA:	RHA1_ro05698

RER:	RER_07380 RER_33860 RER_43230

REY:	O5Y_03585 O5Y_15605 O5Y_20260

REB:	XU06_03825 XU06_15840 XU06_20430

RQI:	C1M55_04070 C1M55_16855 C1M55_21775

ROP:

ROP_57660

ROA:	Pd630_LPD02312

RPY:	Y013_20155

RBY:	CEJ39_06655

RAV:	AAT18_05750 AAT18_07275

RHW:	BFN03_01840

RRZ:	CS378_20980 CS378_22345

RHQ:	IM25_09985

RHOD:

AOT96_01820 AOT96_14465 AOT96_27040

RRT:	4535765_00633(ifcA_1) 4535765_00838(ifcA_2)

RCR:	NCTC10994_01879(ifcA_1) NCTC10994_02087(ifcA_2) NCTC10994_03292(ifcA_5)

RTM:	G4H71_04820 G4H71_07430

RKO:	JWS14_31210

RGO:	KYT97_05695 KYT97_15530 KYT97_16405 KYT97_23105 KYT97_28850

ROZ:	CBI38_22585

RPSK:

JWS13_13090

RGOR:

NMQ04_02775 NMQ04_03645 NMQ04_22220

RHOP:

D8W71_07900 D8W71_26930

REQ:	REQ_07930 REQ_08850 REQ_12430 REQ_13730

PDEF:

P9209_25190 P9209_28460

GBR:

Gbro_4545

GPO:	GPOL_c05040

GOR:

KTR9_4518

GOQ:	ACH46_10190 ACH46_19775

GTA:	BCM27_04400 BCM27_23650

GOC:	CXX93_03785

GIT:	C6V83_00900

GRU:	GCWB2_04130(ifcA1) GCWB2_22595(ifcA3)

GOM:	D7316_02580(urdA) D7316_03032(ifcA)

GAV:	C5O27_05830

GOD:	GKZ92_21595

GAM:	GII34_01515

SPIN:

KV203_10735

TPR:

Tpau_3846

TSM:	ASU32_08060 ASU32_21910

TPUL:

TPB0596_41870 TPB0596_44260

TSD:	MTP03_16260 MTP03_43310

DTM:	BJL86_0019

DIT:	C3V38_06510

DIZ:	CT688_00070

DPC:	A6048_00070

DLU:	A6035_00120

DKN:	NHB83_00065

CBQ:	AL705_03940

TOY:	FO059_01500 FO059_06620 FO059_16680

SALU:

DC74_8142

SALL:

SAZ_42040

SNK:	CP967_03275

SXN:	IAG42_07600

SRUG:

F0345_00800

STUB:

MMF93_31680

AARC:

G127AT_04070

ARL:	AFL94_08135

ARY:	ATC04_09765

AJG:	KKR91_06375

ASUN:

KG104_04150

ACAO:

NF551_01370

GLU:	F0M17_08150

GPR:	JQN66_08300

GMY:

XH9_00545

GNC:	QQS42_08160

NCA:

Noca_0296

NDK:	I601_0577(urdA) I601_1513(ifcA_1)

NBE:	Back2_26880

NANO:

G5V58_21245

NARO:

CFH99_22010

NPI:	G7071_12495

NPC:	KUV85_11715

PSIM:

KR76_13710 KR76_24505

AEB:	C6I20_03515

TCU:

Tcur_3515

ACTW:

F7P10_05400 F7P10_28365

AMAZ:

LUW76_36070

ACIR:

OG979_26450

ACTQ:

OG417_40235

FAL:

FRAAL3038

AMD:

AMED_2521

AMN:

RAM_12815

AMM:

AMES_2493

AMZ:

B737_2494

AOI:

AORI_2483

AJA:	AJAP_26410

AMQ:	AMETH_4839

AMYC:

CU254_10455 CU254_23445

AMYB:

BKN51_05015

AAB:	A4R43_16050

AMYY:

YIM_14445 YIM_36750(urdA)

AORI:

SD37_13480

AACD:

LWP59_23765 LWP59_29205

APRT:

MUY14_23835

AROO:

NQK81_09935

ATHM:

L1857_26970

ARHD:

VSH64_01775

KAL:

KALB_5139

KUT:	JJ691_07860

KPHY:

AOZ06_42835

LED:	BBK82_38945

ALO:

CRK59602

PMAD:

BAY61_10950

SACE:

GIY23_13360

CROS:

N8J89_29395

STP:	Strop_2675

SAQ:

Sare_2883

AMS:	AMIS_19130

AIH:	Aiant_18970

ACTL:

L3i22_084550

ASIC:

Q0Z83_065660

AOU:	ACTOB_004112

DFU:	Dfulv_21840

JCY:	M6B22_17710 M6B22_21615

ABAI:

IMCC26256_11535

» show all

Reference

1 [PMID:13673006]

Authors

LEVY HR, TALALAY P.

Title

Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation.

Journal

J Biol Chem 234:2014-21 (1959)

Reference

2 [PMID:8655514]

Authors

Florin C, Kohler T, Grandguillot M, Plesiat P

Title

Comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.

Journal

J Bacteriol 178:3322-30 (1996)
DOI:10.1128/JB.178.11.3322-3330.1996

Sequence

[ag:AAB08517]

Reference

3 [PMID:12902225]

Authors

Horinouchi M, Hayashi T, Yamamoto T, Kudo T

Title

A new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441 which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes.

Journal

Appl Environ Microbiol 69:4421-30 (2003)
DOI:10.1128/AEM.69.8.4421-4430.2003

Sequence

[ag:BAC81693]

Other DBs

ExplorEnz - The Enzyme Database:

1.3.99.5

IUBMB Enzyme Nomenclature:

1.3.99.5

ExPASy - ENZYME nomenclature database:

1.3.99.5

BRENDA, the Enzyme Database:

1.3.99.5

CAS:

9036-43-5

DBGET integrated database retrieval system