KEGG ENZYME: 1.3.99.8

ENZYME: 1.3.99.8

Entry

EC 1.3.99.8                 Enzyme

Name

2-furoyl-CoA dehydrogenase;
furoyl-CoA hydroxylase;
2-furoyl coenzyme A hydroxylase;
2-furoyl coenzyme A dehydrogenase;
2-furoyl-CoA:(acceptor) 5-oxidoreductase (hydroxylating)

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors

Sysname

2-furoyl-CoA:acceptor 5-oxidoreductase (hydroxylating)

Reaction(IUBMB)

2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor [RN:R02987]

Reaction(KEGG)

R02987

Substrate

2-furoyl-CoA [CPD:C00845];
H2O [CPD:C00001];
acceptor [CPD:C00028]

Product

S-(5-hydroxy-2-furoyl)-CoA [CPD:C03724];
reduced acceptor [CPD:C00030]

Comment

A copper protein. The oxygen atom of the -OH produced is derived from water, not O2; the actual oxidative step is probably dehydrogenation of a hydrated form -CHOH-CH2- to -C(OH)=CH-, which tautomerizes non-enzymically to -CO-CH2-, giving (5-oxo-4,5-dihydro-2-furoyl)-CoA. Methylene blue, nitro blue, tetrazolium and a membrane fraction from Pseudomonas putida can act as acceptors.

History

EC 1.3.99.8 created 1976

Pathway

ec00365

Furfural degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K16877

2-furoyl-CoA dehydrogenase large subunit

K16878

2-furoyl-CoA dehydrogenase FAD binding subunit

K16879

2-furoyl-CoA dehydrogenase 2Fe-2S iron sulfur subunit

Genes

PWI:	MWN52_02905 MWN52_02910 MWN52_02915

PPSE:

BN5_2298(hmfA) BN5_2299(hmfB) BN5_2300(hmfC)

PMAN:

OU5_0887 OU5_0888 OU5_0889

PPUU:

PputUW4_02623 PputUW4_02624 PputUW4_02625

PSIL:

PMA3_09770 PMA3_09775 PMA3_09780

PALL:

UYA_10530 UYA_10535 UYA_10540

PSTT:

CH92_11530 CH92_11535 CH92_11540

HSV:	HNO53_04195 HNO53_04200 HNO53_04205

HPRO:

LMS44_09430 LMS44_09435 LMS44_09440

ALCA:

ASALC70_04230(cdhC_2) ASALC70_04231(kdhA_2) ASALC70_04232(cdhA_2)

ADI:	B5T_02845 B5T_02846 B5T_02847

RPI:	Rpic_2052 Rpic_2053 Rpic_2054

RPF:	Rpic12D_1744 Rpic12D_1745 Rpic12D_1746

RPJ:	N234_37060 N234_37065 N234_37070

REH:	H16_A1701(coxS3) H16_A1702(h16_A1702) H16_A1703(coxL3)

CUH:	BJN34_36275 BJN34_36280 BJN34_36285

REU:	Reut_C5888 Reut_C5889 Reut_C5890

COX:	E0W60_18630 E0W60_18635 E0W60_18640 E0W60_32410 E0W60_32415 E0W60_32420

CUK:	KB879_34365 KB879_34370 KB879_34375

BAEN:

L3V59_43060 L3V59_43065 L3V59_43070

BUG:	BC1001_3787 BC1001_3788 BC1001_3789

BGF:	BC1003_0590 BC1003_0591 BC1003_0592

BYI:	BYI23_A013020 BYI23_A013030 BYI23_A013040

BUQ:	AC233_20840 AC233_20845 AC233_20850

BUM:	AXG89_18115 AXG89_18120 AXG89_18125

BUI:	AX768_27890 AX768_27895 AX768_27900

BXE:	Bxe_A2073 Bxe_A2074 Bxe_A2075

BXB:	DR64_4228 DR64_4229 DR64_4230(qorS)

BPH:	Bphy_6671 Bphy_6672 Bphy_6673

BGE:	BC1002_5745 BC1002_5746 BC1002_5747

BPX:	BUPH_00361 BUPH_00362 BUPH_00363

BPY:	Bphyt_2183 Bphyt_2184 Bphyt_2185

BUZ:	AYM40_12165 AYM40_12170 AYM40_12175

BFN:	OI25_3188 OI25_3189 OI25_3190

BCAI:

K788_0001305 K788_0001306 K788_0001307

PARB:

CJU94_33600 CJU94_33605 CJU94_33610

PHS:	C2L64_39380 C2L64_39385 C2L64_39390

PTER:

C2L65_36660 C2L65_36665 C2L65_36670

PGP:	CUJ91_22575 CUJ91_22580 CUJ91_22585

PCJ:	CUJ87_08680 CUJ87_08685

PDIO:

PDMSB3_2266(cutC) PDMSB3_2267 PDMSB3_2268

PSAA:

QEN71_39410 QEN71_39415 QEN71_39420

PKF:	RW095_28685 RW095_28690 RW095_28695

PDQ:	CL55_00008510 CL55_00008520 CL55_00008530

CABA:

SBC2_58950 SBC2_58960(cdhB_2) SBC2_58970(cdhC)

CABK:

NK8_77700 NK8_77710 NK8_77720

BPE:

BP0684A

BPC:

BPTD_0692

BPER:

BN118_3020

BPET:

B1917_0471

BPEU:

Q425_4310

BPAR:

BN117_0386

BPA:

BPP0389

BBH:	BN112_3022

BBR:

BB0391

BBM:	BN115_0376

BBX:	BBS798_0387

BAV:	BAV2737(cutS)

BBRO:

BAU06_03095 BAU06_03100 BAU06_03105 BAU06_14970 BAU06_14975

BFZ:	BAU07_03945 BAU07_13310 BAU07_13315 BAU07_13320 BAU07_17240 BAU07_17245 BAU07_17250

BOH:	AKI39_05190 AKI39_05195 AKI39_05200 AKI39_11050 AKI39_14205

BGM:	CAL15_18105

BGV:	CAL12_03280 CAL12_03305 CAL12_03310 CAL12_03315 CAL12_15640 CAL12_15825 CAL12_15830 CAL12_15835

ACHR:

C2U31_19620

AMIM:

MIM_c34420 MIM_c34430 MIM_c34440

AFA:	UZ73_14445

ODI:	ODI_R0528 ODI_R0529 ODI_R0530

PIG:	EGT29_02200 EGT29_02790 EGT29_14620 EGT29_14625 EGT29_14630

RHOB:

HTY51_03285 HTY51_03290 HTY51_03295

ACK:	C380_16250 C380_16255 C380_21260 C380_21265 C380_21270

LIM:	L103DPR2_01710(kdhA)

HPSE:

HPF_15720(cdhA) HPF_15725(kdhA) HPF_15730(cdhC)

HCZ:	G9Q37_00905 G9Q37_00910 G9Q37_00915 G9Q37_18270

ATER:

MW290_03380 MW290_03385 MW290_03390

MVAR:

MasN3_29740(cutL) MasN3_29750(cutM) MasN3_29760

TMJ:	P0M04_26565 P0M04_26570 P0M04_26575

ATW:	C0099_09885 C0099_09890 C0099_09895

AMIS:

Amn_pb01240

ORM:	HTY61_00440 HTY61_00445 HTY61_00450

KMN:	HW532_18830 HW532_18835 HW532_18840

RHI:	NGR_c05960

SAME:

SAMCFNEI73_pC1495

EAK:	EKH55_3886 EKH55_3887 EKH55_3888

ASAL:

CFBP5507_25025

REI:	IE4771_PA00054

RGA:	RGR602_PA00038(coxM-2)

RHN:	AMJ98_PC00054

REZ:	AMJ99_PB00053

NPM:	QEO92_15500 QEO92_15505 QEO92_15510

ABAW:

D5400_18155 D5400_18160 D5400_18165

BJA:	blr3533(cutL) blr3534(cutM) blr3535(blr3535)

BJU:	BJ6T_61950 BJ6T_61960 BJ6T_61970

BJP:	RN69_30010 RN69_30015 RN69_30020

BOT:	CIT37_36540 CIT37_36545 CIT37_36550

BGQ:	X265_16165 X265_16170 X265_16175

BARH:

WN72_17955 WN72_17960 WN72_17965

BEL:	BE61_31160(coxM)

BXN:	I3J27_12855 I3J27_12860 I3J27_12865

BROS:

QUH67_23250

RPC:	RPC_0848 RPC_0849 RPC_0850

VGO:	GJW-30_1_04058(cdhC_3)

BOS:	BSY19_1058 BSY19_1059 BSY19_1060

BVV:	BHK69_28550 BHK69_28555 BHK69_28560

BOF:	FQV39_09960

BVES:

QO058_30845 QO058_30850

XDI:	EZH22_10480 EZH22_10485 EZH22_10490

MRD:	Mrad2831_4723 Mrad2831_4724 Mrad2831_4725

MET:	M446_1301 M446_1302 M446_1303

MNO:	Mnod_3296 Mnod_3297 Mnod_3298

MOR:	MOC_3637 MOC_3638 MOC_3639

MAQU:

Maq22A_c13985(coxS) Maq22A_c13990(coxM) Maq22A_c13995(coxL)

MPHY:

MCBMB27_05472 MCBMB27_05473 MCBMB27_05474

MEE:	DA075_33360 DA075_33365 DA075_33370

METX:

A3862_04985 A3862_04990 A3862_04995

METI:

DK427_10980 DK427_10985 DK427_10990

RHZ:	RHPLAN_07920 RHPLAN_07930 RHPLAN_07940

PSIN:

CAK95_25375 CAK95_25380 CAK95_25385 CAK95_28855

RBM:

TEF_08695

DSH:	Dshi_3719(coxL) Dshi_3720(coxM) Dshi_3721(coxS)

PABY:

Ga0080574_TMP3693 Ga0080574_TMP3694 Ga0080574_TMP3695

SULD:

B5M07_03660 B5M07_03665 B5M07_03670

SPOT:

G6548_00070 G6548_00075 G6548_00080

RID:	RIdsm_04272(cdhA) RIdsm_04273(cdhB) RIdsm_04274(cdhC_3)

LALG:

LentiSH36_02839 LentiSH36_02840 LentiSH36_02841

PTP:	RCA23_c12060 RCA23_c12070 RCA23_c12080

CMAG:

CBW24_14190 CBW24_14195 CBW24_14200

SMIC:

SmB9_31320

ACR:	Acry_0668 Acry_0669 Acry_0670

AMV:	ACMV_11990 ACMV_12000 ACMV_12010

AZL:	AZL_a11000 AZL_a11010(cutM) AZL_a11020(xdhB)

ATI:	AL072_24270 AL072_24275 AL072_24280

AOZ:	HUE56_07955 HUE56_07960 HUE56_07965

HTQ:	FRZ44_10690

HADH:

FRZ61_10370

BACQ:

DOE78_11790 DOE78_11800

BKO:	CKF48_20745

CFIR:

NAF01_14290

GKA:	GKP18 GKP19 GKP21

GMC:	GY4MC1_2423 GY4MC1_2425

GGH:	GHH_c13560(cutL) GHH_c13580

PTL:	AOT13_10300 AOT13_10310

PCAL:

BV455_00386(kdhA) BV455_00388(cdhA)

PARG:

PspKH34_23880 PspKH34_23900(cutL)

ANM:	GFC28_297 GFC28_299

ANL:	GFC29_1325 GFC29_1327

FEC:	QNH15_24100

APAK:

AP3564_10395 AP3564_10405

ACOP:

RI196_05715 RI196_05725

SHV:	AAT16_04525 AAT16_04535 AAT16_04540

PBJ:	VN24_01240

PNP:	IJ22_36520 IJ22_36540

AFX:	JZ786_00980

ACUR:

JZ785_19205

ADAU:

NZD86_05845

BTS:

Btus_2332

KYR:	CVV65_11710

EFF:	skT53_32220(cutL) skT53_32240

SAY:	TPY_2900(cutM)

SAP:	Sulac_0987

MDX:	BTO20_26750

MGOR:

H0P51_10250

MALV:

MALV_28090

RGOR:

NMQ04_20065

SVL:	Strvi_4351 Strvi_4353

SRC:	M271_02660 M271_02670

SLON:

LGI35_43435

PSEI:

GCE65_15275

JAY:	H7A72_04100 H7A72_12330 H7A72_12340

TEH:	GKE56_00145

PEI:	H9L10_02035

TED:	U5C87_00710 U5C87_00720

BLUT:

EW640_00545

NCA:

Noca_1489

NDK:	I601_1311(kdhA)

NOY:	EXE57_06220

NANO:

G5V58_15385

NPS:	KRR39_18325 KRR39_18335

PSIM:

KR76_07345

ACTQ:

OG417_37250 OG417_48800

NOA:	BKM31_01510

NOW:	GBF35_36485

BLAP:

MVA48_02295 MVA48_10745 MVA48_10755

SACC:

EYD13_13315(kdhA)

AJA:	AJAP_06565

AMQ:	AMETH_3079 AMETH_3081(cutM)

AMYB:

BKN51_25850

AACD:

LWP59_20645 LWP59_20655

ARHD:

VSH64_07580 VSH64_07585

PDX:	Psed_5931 Psed_5933 Psed_6475

PSEA:

WY02_08300 WY02_08310 WY02_22155

PSEE:

FRP1_20535

PSEH:

XF36_05370 XF36_23380

PSEQ:

AD006_27955

PECQ:

AD017_07550

PHH:	AFB00_17905

PAUT:

Pdca_02880 Pdca_13730 Pdca_67730(cutL_4) Pdca_67750

PBRO:

HOP40_06535 HOP40_06545

PPEL:

H6H00_04685 H6H00_04690 H6H00_24320 H6H00_24330

SESP:

BN6_44840

KPHY:

AOZ06_23895

ACTY:

OG774_11360 OG774_11370

EKE:	EK0264_00850

BALA:

DSM104299_03661(cdhC)

CWO:	Cwoe_4071 Cwoe_4105

SBAE:

DSM104329_02310(cutS_1) DSM104329_04642(cutL_4)

KBS:	EPA93_17140

HAXY:

NGM07_22130

HMP:	K6T50_16000

HRE:	K6T36_15890

NVR:	FEJ81_19730

SACD:

HS1genome_0527

» show all

Reference

1 [PMID:4655411]

Authors

Kitcher JP, Trudgill PW, Rees JS.

Title

Purification and properties of 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2.

Journal

Biochem J 130:121-32 (1972)
DOI:10.1042/bj1300121

Other DBs

ExplorEnz - The Enzyme Database:

1.3.99.8

IUBMB Enzyme Nomenclature:

1.3.99.8

ExPASy - ENZYME nomenclature database:

1.3.99.8

UM-BBD (Biocatalysis/Biodegradation Database):

1.3.99.8

BRENDA, the Enzyme Database:

1.3.99.8

CAS:

9068-18-2

DBGET integrated database retrieval system