KEGG ENZYME: 1.5.99.12

ENZYME: 1.5.99.12

Entry

EC 1.5.99.12                Enzyme

Name

cytokinin dehydrogenase;
N6-dimethylallyladenine:(acceptor) oxidoreductase;
6-N-dimethylallyladenine:acceptor oxidoreductase;
OsCKX2;
CKX;
cytokinin oxidase/dehydrogenase;
N6-dimethylallyladenine:acceptor oxidoreductase

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With unknown physiological acceptors

Sysname

N6-prenyladenine:acceptor oxidoreductase

Reaction(IUBMB)

N6-prenyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor [RN:R05708]

Reaction(KEGG)

R05708

Substrate

N6-prenyladenine [CPD:C04083];
acceptor [CPD:C00028];
H2O [CPD:C00001]

Product

adenine [CPD:C00147];
3-methylbut-2-enal [CPD:C07330];
reduced acceptor [CPD:C00030]

Comment

A flavoprotein (FAD). Catalyses the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is a prenyl group. Although this activity was previously thought to be catalysed by a hydrogen-peroxide-forming oxidase, this enzyme does not require oxygen for activity and does not form hydrogen peroxide. 2,6-Dichloroindophenol, methylene blue, nitroblue tetrazolium, phenazine methosulfate and copper(II) in the presence of imidazole can act as acceptors. This enzyme plays a part in regulating rice-grain production, with lower levels of the enzyme resulting in enhanced grain production [2].

History

EC 1.5.99.12 created 2001

Pathway

ec00908

Zeatin biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K00279

cytokinin dehydrogenase

Genes

ATH:	AT1G75450(CKX5) AT2G19500(CKX2) AT2G41510(CKX1) AT3G63440(CKX6) AT4G29740(CKX4) AT5G21482(CKX7) AT5G56970(CKX3)

ALY:	9302249 9305487 9308045 9312791 9317846 9322103 9325089

CRB:	17877017 17884533 17885237 17887842 17890111 17892323 17895153

CSAT:

104701434 104702290 104704111 104706475 104713195 104717225 104721893 104726241 104730350 104733218 104733803 104736200 104747884 104748975 104751417 104770512 104782642 104785229 104789862 104793063

EUS:	EUTSA_v10005878mg EUTSA_v10013259mg EUTSA_v10013271mg EUTSA_v10016470mg EUTSA_v10018378mg EUTSA_v10023158mg EUTSA_v10024918mg

BRP:	103249144 103249171 103831996 103842132 103845129 103845596(CKX) 103849258 103851408 103857986 103861901 103865890 103866566

BNA:	106354495 106356750 106370330 106371682 106374136 106384861 106389384 106395144 106396215 106405957 106406332 106409476 106409493 106415860 106417025 106417645 106421388 106437984 106450132 106450297 106450662

BOE:	106300565 106302335 106304349 106307242 106314001 106316377 106319493 106324800 106327746 106328016 106340592 106340923

RSZ:	108811715 108816044 108817875 108824931 108825934 108826378 108840141 108847852 108849518 108852197 130510414 130512880

THJ:	104799579 104801962 104801969 104809069 104810957 104813263 104814623 104819113

CPAP:

110807147 110808166 110809263 110810796 110817106 110824975

CIT:	102611545 102612158 102615244 102615601 102616491 102616868 102628616

CIC:	CICLE_v10000709mg CICLE_v10006491mg CICLE_v10007992mg CICLE_v10007999mg CICLE_v10010210mg CICLE_v10010516mg CICLE_v10010713mg CICLE_v10031210mg

PVY:	116119285 116127456 116135227 116135253 116141198 116143532

MINC:

123194179 123194418 123197203 123200977 123201053 123214226 123216080 123229746

TCC:	18585729 18588149 18588386 18591458 18598855

GRA:	105769336 105769821 105772487 105773753 105774850 105778350 105780879 105783559 105784429 105784502 105784895 105792493 105792958 105799471

GHI:	107887245 107889422 107891330 107893332 107893390 107901110 107905468 107907049 107917563 107918823 107931753 107932015 107932208 107932821 107933262 107935091 107936339 107948716 107957688 107957953 107958799 107962826 121204790 121225177 121230667 121231949

GAB:	108454579 108457453 108459630 108463135 108463283 108463523 108465440 108466723 108468351 108478724 108482141 108484293 108487904

HSYR:

120113595 120117809 120131990 120138530 120138722 120147828 120149217 120153705 120162161 120164690 120169288 120169340 120180068 120185841 120187602 120192282 120192322 120194660 120195285 120211850

DZI:	111285701 111285989 111295472 111295530 111300404 111304129 111304180 111307497 111309704 111311483 111312756

EGR:	104414605 104414741 104417267 104417269 104417272 104417273 104425330 104429159 104436478 104436479 104437929 104448985

GMX:	100777568 100782645 100783374 100787676(CKX3) 100790584 100793158 100797430 100797928 100800722 100804431 100808553 100808619 100808738(CKX1) 100816566 100816815

GSJ:	114366977 114367555 114374357 114380172 114381795 114381835 114384973 114387286 114392639 114393084 114393636 114399635 114406683 114408616 114408858 114414628 114425863

PVU:	PHAVU_001G038800g PHAVU_001G128800g PHAVU_003G136400g PHAVU_003G1365000 PHAVU_005G034000g PHAVU_009G060200g PHAVU_009G081800g PHAVU_009G231700g PHAVU_009G231800g PHAVU_011G014000g

VRA:	106752528 106759647 106760070 106761132 106761356 106762749 106765121 106766499 106767266 106779231

VAR:	108322687 108322709 108329862 108330146 108330147 108330947 108331006 108333575 108338740 108339906 108343582

VUN:	114162232 114163794 114164020 114164552 114168359 114175071 114176603 114177218 114194748 114195390

VUM:	124823603 124823874 124826179 124832925 124839312 124842513 124842932 124842938 124843758 124846856

CCAJ:

109791581 109791932 109793095 109798538 109800322 109801340 109802927 109807493 109807518 109811464 109815775 109815776 109815777 109815783 109815785 109816117

APRC:

113846270 113848196 113852993 113863018 113864799 113865109 113865371 113866235 113874330 113874738

MTR:	11415465 11427830 11431926 11439864 11443256 11444937 25490232 25494228 25494229

TPRA:

123882891 123893226 123897134 123906086 123906087 123914468 123916562 123920590

CAM:	101490796 101491048 101493024 101493173 101493924 101495126 101505170 101506131 101511173 101511484

PSAT:

127079432 127082854 127091608 127105500 127115753 127126366 127135127 127135129 127135851 127136512

VVO:	131595047 131596382 131599456 131601553 131601563 131606619 131610304 131611622 131631486 131631487 131639183 131639186 131657075 131659505 131660205 131660225

LJA:

Lj0g3v0360459.1(Lj0g3v0360459.1) Lj1g3v4226830.1(Lj1g3v4226830.1) Lj3g3v0809870.1(Lj3g3v0809870.1) Lj3g3v3650360.1(Lj3g3v3650360.1) Lj4g3v2171460.1(Lj4g3v2171460.1) Lj4g3v2641050.1(Lj4g3v2641050.1) Lj5g3v0434840.1(Lj5g3v0434840.1) Lj5g3v0692300.1(Lj5g3v0692300.1) Lj5g3v2238680.1(Lj5g3v2238680.1) Lj6g3v1249630.1(Lj6g3v1249630.1)

ADU:	107460029 107470669 107477483 107478835 107492593 107495642 107496676 107496684 107496695 107496704 107496718

AIP:	107605478 107610058 107624095 107629652 107630383 107643097 107643105 107643115 107643130 107647926

AHF:	112695991 112697094 112697106 112697113 112697117 112697642 112705586 112716071 112723638 112723639 112723640 112723641 112724135 112737300 112738221 112755883 112759190 112764801 112784141 112789376 112790353

LANG:

109325241 109326244 109326311 109326988 109328504 109328506 109334683 109336323 109338365 109339610 109340791 109343620 109343948 109348201 109352813 109358665 109361578 109361687

PCIN:

129296063 129302220 129302231 129305969 129309511 129310126 129313503 129318233

QSA:	O6P43_001421 O6P43_002754 O6P43_002755 O6P43_006465 O6P43_016218 O6P43_018623 O6P43_022419 O6P43_022420 O6P43_029631

FVE:	101291035 101294496 101295539 101300743 101302014 101302306 101310777 105350274

RCN:	112168160 112173217 112173709 112189446 112190933 112191661

PPER:

18770627 18770925 18771839 18784595 18791737 18793759

PMUM:

103320663 103321322 103330694 103340690 103340727 103342437

PAVI:

110749240 110749268 110749425 110756141 110771543 110773015

PDUL:

117616553 117619030 117633555 117634236 117635184 117635502

MDM:	103401941 103412527 103424196 103431537 103438473 103440288 103440905 103450431 103454534 103455779 108172967

MSYL:

126592609 126600472 126601093 126602532 126605104 126605105 126605762 126611151 126628434 126631883 126632470

PXB:	103927480 103928445 103929168 103929171 103940781 103944001 103944039 103945547 103951949 103952784 103953218 103953221 103957629

ZJU:	107417809 107420171 107420502 107423943 107423992 107424035 107431600 125422040

MNT:	21391993 21399606 21401563 21404740 21409908 21409909

CSAV:

115703213 115704143 115705068 115710080 115711707

CSV:	101205347 101207014 101212272 101217722 101217899 101222481 101222887 105434535

CMO:	103486946 103488003 103488756 103492330 103492529 103493699 103498521 103502439

BHJ:	120071295 120071717 120074573 120076437 120080114 120081608 120086338 120091742

MCHA:

111010228 111011644 111013907 111016468 111016479 111021281 111021923 111022941

CMAX:

111465505 111465981 111466785 111467189 111477202 111481206 111482266 111485168 111485172 111485722 111492293 111493700 111496174 111496957

CMOS:

111432444 111434108 111435447 111440877 111441651 111442782 111444271 111446693 111446933 111450699 111454619 111458137 111461626 111461627 111462337

CPEP:

111780058 111780059 111781792 111785053 111788155 111789088 111789946 111792967 111793941 111796710 111799187 111799248 111803163 111804846 111805242 111809842

RCU:	8258580 8259348 8272556 8272618 8275787

JCU:	105631179 105633857 105637889 105638086 105645275 105646199 105646261

HBR:	110632633 110638818 110643050 110655159 110656154 110661153 110662014 110662016 110662237 110665307 110666594 110671612

MESC:

110603861 110604114 110607020 110610430 110612191 110613873 110616142 110618170 110620659 110623472

POP:	18094319 7462570 7462767 7468923 7477637 7479715 7492296 7496121 7497623

PEU:	105114882 105119115 105119506 105122124 105128792 105131757 105134910 105137887 105142254 105142748

PALZ:

118028801 118030857 118045583 118047988 118052599 118052772 118053152 118054316 118062100

JRE:	108985996 108988580 108991365 108995142 108995155 109005247 109008387 109009322 109009522 109009530 109010570 118349863

CILL:

122275492 122279126 122292044 122293909 122297322 122304352 122304353 122305688 122307894 122307999 122314834

CAVE:

132161642 132166383 132166434 132166621 132168126 132184937 132190518

QSU:	111997861 111999686 112004285 112009080 112014938 112015136 112023224

QLO:	115953343 115954861 115973121 115974410 115974767 115978134 115983505

TWL:	119989542 119996053 119997676 119998308 120000485 120003143 120006404 120006647 120009653

VVI:	100242449 100245801 100256802 100260570 100263730 100265401 100267760 100852458 100853726 100854562

VRI:	117907962 117913384 117917363 117917767 117918143 117919997 117924875 117928716

SLY:	100874535(CKX5) 100874536(CKX7) 100874537(CKX2) 101055579 101055598(CKX1) 101055612(CKX4) 101244307(CKX3) 101255039 101266491

SPEN:

107002172 107006755 107006947 107015845 107016001 107016377 107026651 107028037

SOT:	102577493(CKX3) 102577533(CKX5) 102577731(CKX4) 102577758 102577888 102605765 102605861

SSTN:

125842548 125842549 125853398 125855041 125856699 125862954 125862964 125864667

SDUL:

129870481 129870546 129877050 129887025 129888293 129889180 129903460

CANN:

107841095 107841831 107843853 107852795 107854914 107855627 107864236 107866953 107870932

LBB:	132602050 132605487 132613752 132618102 132628065 132635977 132636882 132636886 132638797 132638798 132644832

NTA:	107767396 107778197 107786307 107790677 107791003 107793379 107799019 107800706 107800769 107801329 107814889 107816221 107817189 107818107

NSY:	104213285 104218779 104230796 104231578 104232618 104240791 104245285

NTO:	104087533 104090136 104097491 104103263 104103455 104103846 104120662

NAU:	109207622 109211291 109217037 109226073 109226138 109231758 109232925

INI:	109148184 109148185 109150353 109151199 109152883 109159043 109180768 109180886 109186200 109190913

ITR:	116000251 116000498 116005467 116006468 116014165 116017596 116019993 116026732 116028775 116031022

SIND:

105159857 105161857 105161858 105162564 105164613 105166085 105169465 105173624 105173822 105173823 105180056

OEU:	111370301 111381252 111384629 111386059 111386064 111388513 111390813 111390817 111390818 111397162 111397578 111399653 111403866 111408921 111410497

EGT:	105949413 105958400 105962980 105966324 105969568 105976686 105977200

SSPL:

121747090 121755660 121759611 121770987 121780259 121781802 121791537 121793782 121795177 121797074 121798787 121800394 121801132 121806581

SMIL:

130986529 130990174 131001647 131004921 131015369 131022358

SHIS:

125189293 125189430 125191846 125197211 125197440 125197472 125205780 125215838 125216365 125222800

APAN:

127246778 127246885 127251545 127251954 127252117 127259055 127262002 127265690

HAN:	110867135 110877038 110896212 110897998 110928459 110928970 110930542 110935805 110941420

ECAD:

122579369 122582599 122582743 122596726 122600679 122602418 122607856

LSV:	111880945 111888556 111889585 111892654 111905559 111915756

CCAV:

112500839 112501207 112511556 112513307 112514498 112516771

DCR:	108192283 108201369 108205173 108207300 108211300 108211529 108214428 108215649 108222095 108223746 108225601

CSIN:

114256616 114256695 114278252 114286666 114292569 114295033

RVL:	131318584 131318604 131320066 131330979 131334711

AEW:	130756214 130757427 130758573 130780544 130781029 130783369 130784358 130785995 130789123 130789908 130795323

BVG:	104884992 104886566 104887446 104889207 104889407 104909026

SOE:	110790574 110795101 110803190 110804981 110805199

CQI:	110683076 110691570 110694024 110704399 110705378 110707353 110710152 110712034 110712896 110716608 110723287 110724757

ATRI:

130798907 130801962 130804078 130811413 130813529 130815123 130815448 130826116

MOF:	131152928 131157605 131163093 131163952 131167458 131167459 131167460 131167797 131168083

NNU:	104586639 104590176 104590177 104595411 104595590 104600585 104600703 104606255 104610555 104613458

MING:

122061763 122064057 122068735 122075595 122076055 122077405 122078906 122079156 122085214 122090831 122092951

TSS:	122642765 122654447 122654960 122657041 122657476 122665182 122666916 122669552 122672771

PSOM:

113298257 113303265 113335746 113347449 113347647 113347832 113352879 113357260

OSA:	107275724 107275892 107275925 107276418 4325668 4326515 4327333 4327887 4338605 4345764 4348932

DOSA:

Os01t0187600-01(Os01g0187600) Os01t0197700-01(Os01g0197700) Os01t0775400-01(Os01g0775400) Os01t0940000-01(Os01g0940000) Os02t0220000-00(Os02g0220000) Os02t0220100-00(Os02g0220100) Os04t0523500-00(Os04g0523500) Os05t0374200-00(Os05g0374200) Os06t0572300-00(Os06g0572300) Os10t0483500-01(Os10g0483500)

OBR:	102701681 102702986 102707087 102707376 102708057 102711882 102715878 102719344 102719989 102721174

OGL:	127752827 127755026 127755391 127763033 127764107 127767439 127771588 127772659 127777676 127781609 127783183

BDI:	100821104 100821608 100825123 100825364 100825887 100826954 100833368 100834512 100835621 100839711 100843361

ATS:	109733489 109736725 109737583 109739344 109740122 109751588 109751596 109755232 109756036 109757480 109759733 109759953 109769177 109772148 109775285 109775286 109784599

TDC:	119267447 119269181 119269523 119270412 119271344 119275382 119277145 119277171 119278546 119280955 119281068 119320081 119326022 119330511 119333514 119334480 119338452 119339035 119341166 119355824 119364963

TAES:

100682455 100682456 101290595 101290604 101290606 101290671 123046080 123046279 123053593 123053961 123057975 123058082 123058083 123062219 123063416 123065141 123070964 123072395 123074137 123074275 123074281 123079353 123080639 123121802 123130341 123130577 123134105 123149681 123154057 123157354 123158090 123165939 123169883 123181110 123181824 542876

TUA:	125514910 125517370 125519722 125523144 125531145 125539106 125539122 125544959 125546072 125547993 125548075

LPER:

127300938 127309609 127309612 127311881 127316621 127318545 127335639 127341540 127341843 127344584 127344585 127345675 127345676

LRD:	124660858 124666428 124666579 124668017 124668126 124669414 124675170 124675514 124677328 124680225 124680626 124680627 124683700 124683718 124689928 124689929 124689930 124701521 124707774

SBI:	8056020 8067400 8076815 8078740 8079302 8080651 8081320 8081535 8085530 8085531 8085661

ZMA:	100272805 100280446 100526747 100526749 100526750 100526752 100526753 103640050 103641859 103654801 542056 542507 542585

SITA:

101754232 101764621 101769142 101770056 101777262 101777277 101778401 101779751 101780557 101782875

SVS:	117839989 117849954 117854039 117854706 117854955 117855678 117855716 117862053 117863934 117864264

PVIR:

120642423 120650729 120651285 120653949 120670643 120672730 120675078 120676356 120676867 120679351 120693505 120698891 120705692 120705744 120708230 120709213 120712569

PHAI:

112874998 112877933 112886763 112889908 112891447 112894352 112894412 112895611 112897803 112899822

PDA:	103696789 103703401 103704900 103711747 103712017 103712865 103717796 103718641 103722846 103724327 103724330 120105956

EGU:	105034257 105034323 105034779 105038772 105040417 105041828 105045337 105053099 105053404 105057678 105059208 105059463

MUS:	103969437 103976226 103977019 103977698 103979527 103981992 103982995 103984521 103985766 103985902 103986508 103990640 103990857 103991146 103991212 103991774 103993903 103995281 103998247

ZOF:

121967385 121979802 121980446 121983300 121984355 121984891 121985948 121988745 121989900 121992830 121993700 121996797 121997678 122001289 122005582 122007220 122007901 122013250 122015854 122019920 122021879 122023396 122023740 122025725 122040922 122043555 122043987 122043996 122044012 122044023 122045431 122050720 122052597

DCT:	110094204 110095507 110102256 110105324 110106158 110107036 110111299

PEQ:	110017822 110023168 110024452 110026813 110027828 110032648 110033843

AOF:	109826002 109836672 109837834 109843290 109845268 109850514

MSIN:

131223479 131225150 131236894 131242987 131250874 131251699

NCOL:

116245685 116246068 116247467 116255502 116255923 116255927 116261522

ATR:	18429344 18433329 18437669 18439420 18439768 18444385

SMO:	SELMODRAFT_106833(CKX2-2) SELMODRAFT_127173(CKX1-2) SELMODRAFT_174721(CKX2-1) SELMODRAFT_98722(CKX1-1)

PPP:	112273101 112275628 112276058 112276425 112285956 112285983

NGR:	NAEGRDRAFT_82519

PRR:	AT705_12425

PVB:	J5X90_05895

TVL:	FAZ95_28630

NPM:	QEO92_25185

BIC:	LMTR13_29080

BARH:

WN72_41640

BAUT:

QA635_09795

SNO:

Snov_1749

MXA:

MXAN_6144

MSD:	MYSTI_01610

MFB:	MFUL124B02_11430

MYX:	QEG98_34435

CCX:	COCOR_02895(fas5)

AVM:	JQX13_31245

MBD:	MEBOL_006475 MEBOL_007005

CFUS:

CYFUS_003306

CCRO:

CMC5_021910

MRM:	A7982_09810 A7982_13968

SAMY:

DB32_001283

LLU:	AKJ09_00312

NBR:	O3I_021220

NTP:	CRH09_17900

NOZ:	DMB37_35885

ROA:	Pd630_LPD04162

RFA:	A3L23_04946(fas5)

SGB:

WQO_28935

SCB:	SCAB_79151

SSOI:

I1A49_01170

SPRI:

SPRI_0167 SPRI_7186

SPHW:

NFX46_25220

SAUO:

BV401_01400

SPUN:

BFF78_03030

SGD:	ELQ87_37785

SQZ:	FQU76_00475 FQU76_33265

SGZ:	C0216_11910

SGAL:

CP966_03135

SVR:	CP971_00790

SAQU:

EJC51_41790

SSUB:

CP968_00595

SPHV:

F9278_05205 F9278_43495

STSU:

B7R87_00370

SCHA:

CP983_02270

SBAT:

G4Z16_13140

SBRO:

GQF42_40040

SCYG:

S1361_36000(fas5)

SPEU:

CGZ69_35185

SROI:

IAG44_35015

SLF:	JEQ17_05545

SDEC:

L3078_06655

SAKB:

K1J60_38745

SSPN:

LXH13_34125

SDRZ:

NEH16_32380

SINN:

ABB07_02250

AGLA:

OIE69_09170

RIA:	C7V51_11600

NGV:	CDO52_14525

STRR:

EKD16_15700(fas2)

TCU:

Tcur_1966

AGRA:

AGRA3207_007066

AMAZ:

LUW76_43600

ACIR:

OG979_02850

SPIQ:

OHA34_09340

GOB:

Gobs_1829

SEN:

SACE_6632

SACC:

EYD13_04585(fas2)

AOI:

AORI_3424

AJA:	AJAP_22090

AAB:	A4R43_39025

AMYY:

YIM_38835(fas5)

AROO:

NQK81_05335

SESP:

BN6_19900

SSYI:

EKG83_20950

KPHY:

AOZ06_46440

ACTA:

C1701_02465

ALO:

CRK58648

PMAD:

BAY61_05945

SACE:

GIY23_11775

SAQ:

Sare_4954

NHY:	JQS43_11110

CAI:

Caci_7235

AMR:

AM1_0780

ACAM:

HRE53_02840

GLJ:

GKIL_3615

ANA:

all0324

NON:	NOS3756_26750 NOS3756_56680

NED:	HUN01_04280

AVA:

Ava_4717

ANN:	EH233_02135

HBQ:	QI031_07725 QI031_11130

NSPH:

BDGGKGIB_02377(fas5)

AEE:	IM676_09355

NCN:	BZZ01_10405

CTHE:

Chro_3699 Chro_3885

HAU:

Haur_4772

KBS:	EPA93_16345

STAE:

HNV11_08805

» show all

Reference

1 [PMID:11168382]

Authors

Galuszka P, Frebort I, Sebela M, Sauer P, Jacobsen S, Pec P

Title

Cytokinin oxidase or dehydrogenase? Mechanism of cytokinin degradation in cereals.

Journal

Eur J Biochem 268:450-61 (2001)
DOI:10.1046/j.1432-1033.2001.01910.x

Reference

2 [PMID:15976269]

Authors

Ashikari M, Sakakibara H, Lin S, Yamamoto T, Takashi T, Nishimura A, Angeles ER, Qian Q, Kitano H, Matsuoka M

Title

Cytokinin oxidase regulates rice grain production.

Journal

Science 309:741-5 (2005)
DOI:10.1126/science.1113373

Sequence

[dosa:Os01t0197700-01]

Other DBs

ExplorEnz - The Enzyme Database:

1.5.99.12

IUBMB Enzyme Nomenclature:

1.5.99.12

ExPASy - ENZYME nomenclature database:

1.5.99.12

BRENDA, the Enzyme Database:

1.5.99.12

CAS:	55326-39-1

DBGET integrated database retrieval system