Entry
Name
nitric oxide reductase [NAD(P)+, nitrous oxide-forming];
fungal nitric oxide reductase;
cytochrome P450nor;
NOR (ambiguous)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
nitrous oxide:NAD(P) oxidoreductase
Reaction(IUBMB)
N2O + NAD(P)+ + H2O = 2 NO + NAD(P)H + H+ [RN:
R02492 R09446 ]
Reaction(KEGG)
Substrate
Product
Comment
A heme-thiolate protein (P-450). The enzyme from Fusarium oxysporum utilizes only NADH, but the isozyme from Trichosporon cutaneum utilizes both NADH and NADPH. The electron transfer from NAD(P)H to heme occurs directly, not requiring flavin or other redox cofactors.
History
EC 1.7.1.14 created 2011
Pathway
ec01120 Microbial metabolism in diverse environments
Orthology
K15877 fungal nitric oxide reductase
Genes
NTE : NEUTE1DRAFT117740(NEUTE1DRAFT_117740)
NHE : NECHADRAFT_41997 NECHADRAFT_89386 NECHADRAFT_97205
FFC : NCS54_00765400 NCS54_01319500 NCS54_01471300
FKR : NCS57_00805400 NCS57_01306900
CCAC : CcaHIS019_0312330(CcaverHIS019_0312330)
» show all
Taxonomy
Reference
Authors
Shoun H, Tanimoto T
Title
Denitrification by the fungus Fusarium oxysporum and involvement of cytochrome P-450 in the respiratory nitrite reduction.
Journal
J Biol Chem 266:11078-82 (1991)
Sequence
Reference
Authors
Shiro Y, Fujii M, Iizuka T, Adachi S, Tsukamoto K, Nakahara K, Shoun H
Title
Spectroscopic and kinetic studies on reaction of cytochrome P450nor with nitric oxide. Implication for its nitric oxide reduction mechanism.
Journal
Sequence
Reference
Authors
Zhang L, Kudo T, Takaya N, Shoun H
Title
The B' helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH.
Journal
Sequence
Reference
Authors
Oshima R, Fushinobu S, Su F, Zhang L, Takaya N, Shoun H
Title
Structural evidence for direct hydride transfer from NADH to cytochrome P450nor.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.7.1.14
ExPASy - ENZYME nomenclature database: 1.7.1.14