KEGG ENZYME: 2.1.1.113

ENZYME: 2.1.1.113

Entry

EC 2.1.1.113                Enzyme

Name

site-specific DNA-methyltransferase (cytosine-N4-specific);
modification methylase;
restriction-modification system;
DNA[cytosine-N4]methyltransferase;
m4C-forming MTase;
S-adenosyl-L-methionine:DNA-cytosine 4-N-methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:DNA-cytosine N4-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N4-methylcytosine [RN:R03003]

Reaction(KEGG)

R03003

Substrate

S-adenosyl-L-methionine [CPD:C00019];
DNA cytosine [CPD:C00856]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
DNA N4-methylcytosine [CPD:C03110]

Comment

This is a large group of enzymes, most of which, with enzymes of similar site specificity listed as EC 3.1.21.3 (type 1 site-specific deoxyribonuclease), EC 3.1.21.4 (type II site-specific deoxyribonuclease) or EC 3.1.21.5 (type III site-specific deoxyribonuclease), form so-called 'restriction-modification systems'. A complete listing of all of these enzymes has been produced by R.J. Roberts and is available on-line at http://rebase.neb.com/rebase/rebase.html.

History

EC 2.1.1.113 created 1992

Orthology

K00590

site-specific DNA-methyltransferase (cytosine-N4-specific)

Genes

ENF:	AKI40_4424

KOC:	AB185_00150

KOO:	O9K67_21800

XFU:	XFF4834R_plc00230

XPH:	XppCFBP6546_23325(XppCFBP6546P_23325)

XAN:	AC801_22795

AFY:	BW247_13005

NPV:	OHM77_08315

MMEI:

LRP31_33925

SMK:	Sinme_2322

ACUC:

KZ699_07560

RHV:	BA939_16185

PCON:

B0A89_00975

HSH:	NHP194022_06730

GTK:	GT3570_17465

LYP:	MTP04_30080

UTH:	DKZ56_05585

LNG:	BSQ50_12190

STH:

STH334

STHR:

BXT84_01690

HFV:	R50_2020(cfr9IM)

SDIA:

QU667_04650

MGAU:

MGALJ_39500(yhdJ)

SGOB:

test1122_14420

MBIN:

LXM64_03645

DVC:	Dvina_51350

CCOS:

Pan44_35290(dpnA_3)

» show all

Reference

1 [PMID:2172084]

Authors

Kessler C, Manta V.

Title

Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3).

Journal

Gene 92:1-248 (1990)
DOI:10.1016/0378-1119(90)90486-B

Reference

2 [PMID:2690010]

Authors

Klimasauskas S, Timinskas A, Menkevicius S, Butkiene D, Butkus V, Janulaitis A

Title

Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases.

Journal

Nucleic Acids Res 17:9823-32 (1989)
DOI:10.1093/nar/17.23.9823

Sequence

[ag:CAA34887 CAA34854]

Reference

3 [PMID:2159140]

Authors

Roberts RJ.

Title

Restriction enzymes and their isoschizomers.

Journal

Nucleic Acids Res 18 Suppl:2331-65 (1990)
DOI:10.1093/nar/18.suppl.2331

Reference

4 [PMID:6267988]

Authors

Yuan R.

Title

Structure and mechanism of multifunctional restriction endonucleases.

Journal

Annu Rev Biochem 50:285-319 (1981)
DOI:10.1146/annurev.bi.50.070181.001441

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.113

IUBMB Enzyme Nomenclature:

2.1.1.113

ExPASy - ENZYME nomenclature database:

2.1.1.113

BRENDA, the Enzyme Database:

2.1.1.113

CAS:	169592-50-1

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