Entry
Name
thymidylate synthase (FAD);
Thy1;
ThyX
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
5,10-methylenetetrahydrofolate,FADH2:dUMP C-methyltransferase
Reaction(IUBMB)
5,10-methylenetetrahydrofolate + dUMP + NADPH + H+ = dTMP + tetrahydrofolate + NADP+ [RN:
R06613 ]
Reaction(KEGG)
Substrate
Product
Comment
Contains FAD. All thymidylate synthases catalyse a reductive methylation involving the transfer of the methylene group of 5,10-methylenetetrahydrofolate to the C5 position of dUMP and a two electron reduction of the methylene group to a methyl group. Unlike the classical thymidylate synthase, ThyA (EC
2.1.1.45 ), which uses folate as both a 1-carbon donor and a source of reducing equivalents, this enzyme uses a flavin cofactor as a source of reducing equivalents, which are derived from NADPH.
History
EC 2.1.1.148 created 2003, modified 2010
Pathway
Orthology
K03465 thymidylate synthase (FAD)
Genes
RTT : RTTH1527_01420(thyX)
RTB : RTB9991CWPP_01425(thyX)
ERU : Erum6790(probable_thy1)
ERW : ERWE_CDS_07140(thyX)
ERG : ERGA_CDS_07060(thyX)
BSEP : HAP48_0043025(thyX)
BCAN : BcanWSM471_02255(thyX)
AZC : AZC_2577(thyX) AZC_3595
AUA : M673_12545 M673_14310
SPSE : SULPSESMR1_00778(thyX)
RMM : ROSMUCSMR3_00565(thyX)
LALG : LentiSH36_00877(thyX)
LVS : LOKVESSMR4R_00889(thyX)
AALK : LGT41_0004955(thyX)
KOI : LV478_06665(thyX) LV478_11575(thyX)
APER : A0U91_07465 A0U91_16645
RGI : RGI145_12825 RGI145_19470
HPO : HMPREF4655_20125(thyX)
HPX : HMPREF0462_1530(thyX)
HPYA : HPAKL117_07360(thyX)
HPYK : HPAKL86_00215(thyX)
HCL : NCTC13205_00966(thyX)
HSH : NHP194022_00240(thyX)
HPUL : NCTC13154_00015(thyX)
CJV : MTVDSCj20_0025(thyX)
CFV : CFVI03293_1853(thyX)
CAMP : CFT03427_1740(thyX)
CCOC : CCON33237_1844(thyX)
CLR : UPTC16701_0014(thyX)
CLM : UPTC16712_0014(thyX)
CAMR : CAQ16704_0008(thyX)
CHV : CHELV3228_1593(thyX)
CPIN : CPIN18020_1653(thyX)
CAMZ : CVIC12175_0162(thyX)
GBN : GEOBRER4_36200(thyX)
PACE : A6070_11470 A6070_13240
PPOR : JCM14722_29020(thyX)
PBIZ : LWC08_00140(thyX) LWC08_15060(thyX)
SBF : JCM31447_11810(thyX)
BSON : S101395_01163(thyX) S101395_03958
PPQ : PPSQR21_032520(tHY1)
CCOH : SAMEA4530647_2322(thyX)
SMAN : C12CBH8_21300(thyX)
PTH : PTH_0298(THY1) PTH_2150(THY1)
EMT : CPZ25_007085(thyX) CPZ25_007345
BLAU : DQQ01_00695 DQQ01_00810
CSCI : HDCHBGLK_00857(thyX)
CEW : EKH84_06365 EKH84_20205
PDF : CD630DERM_00540(thyX)
PHX : KGNDJEFE_00070(thyX)
TEM : JW646_08890(thyX) JW646_11320(thyX)
TGC : L0P85_16435(thyX) L0P85_18875(thyX)
TMY : TEMA_25160(thyX_1) TEMA_30350(thyX_2)
CDIA : CaldiYA01_05520(thyX)
CNAG : OTJ99_000722(thyX) OTJ99_001876(thyX)
PHAR : NCTC13077_00318(thyX)
PIV : NCTC13079_01480(thyX)
MANA : MAMMFC1_02009(thyX)
PFAC : PFJ30894_01033(thyX)
RTS : CE91St31_12260(thyX)
MBO : BQ2027_MB2775C(thyx)
MMAE : MMARE11_18850(thyX)
MBAI : MB901379_01781(thyX)
MHEK : JMUB5695_03182(thyX)
MKY : IWGMT90018_38690(thyX)
MPHL : MPHLCCUG_03163(thyX)
MTHN : 4412656_02784(thyX)
MHAS : MHAS_01215(thyX_1) MHAS_02391(thyX_2)
MAUU : NCTC10437_02225(thyX)
MAIC : MAIC_20120(thyX_1) MAIC_42480(thyX_2)
MFLV : NCTC10271_03171(thyX)
MSAL : DSM43276_02873(thyX)
MTER : 4434518_02451(thyX)
CDS : CDC7B_1108(thyX1) CDC7B_1468(thyX2)
CDD : CDCE8392_1384(thyX2)
CDIP : ERS451417_01102(thyX1) ERS451417_01490(thyX)
CAR : cauri_1516 cauri_1953(thyX)
CUN : Cul210932_1445(thyX)
CUQ : Cul210931_1333(thyX)
CUJ : CUL131002_1363c(thyX)
CVA : CVAR_0854(thyX1) CVAR_1631(thyX2)
CUV : CUREI_03080 CUREI_11610
CMIN : NCTC10288_00963(thyX)
CRL : NCTC7448_02111(thyX)
CCYS : SAMEA4530656_0884(thyX)
CPSP : L9H27_00830(thyX) L9H27_06275(thyX)
CHEI : CHEID_02355(thyX1) CHEID_05860(thyX2)
CPYR : CYJ47_04890(thyX) CYJ47_06575(thyX)
RCR : NCTC10994_00873(thyX)
RANT : RHODO2019_05140(thyX)
SAMB : SAM23877_5445(thyX) SAM23877_p107(thyX)
SLE : sle1_013 sle_19260(sle_19260)
SALJ : SMD11_1222 SMD11_1860
SBY : H7H31_01130(thyX) H7H31_07530
STUI : GCM10017668_52220(thyX)
SHK : J2N69_16025(thyX) J2N69_27410(thyX)
SANU : K7396_09825(thyX) K7396_34335(thyX)
SGOB : test1122_21065(thyX)
SXT : KPP03845_105436(thyX)
SYUN : MOV08_05095(thyX) MOV08_12895(thyX)
SARG : HKX69_05685 HKX69_09000
SFIY : F0344_08275 F0344_12305
FSB : GCM10025867_23960(thyX)
MALK : MalAC0309_1104(thyX)
PSEV : USB125703_01631(thyX)
ACTW : F7P10_34340(thyX) F7P10_41130
AGRA : AGRA3207_007839(thyX)
NOW : GBF35_21800 GBF35_38115(thyX)
CROS : N8J89_08010(thyX) N8J89_33040(thyX)
MSAG : GCM10017556_40540(thyX)
VMA : VAB18032_12050(thyX)
AEY : CDG81_07710(thyX) CDG81_09725(thyX)
BALA : DSM104299_00741(thyX)
SYR : SynRCC307_2347(thyX)
SYX : SynWH7803_0323(thyX)
SYH : Syncc8109_0272(thyX)
SYNW : SynWH8103_00302(thyx)
SYP : SYNPCC7002_A1309(thyX)
DFO : Dform_00406(thyX|thy1)
OPR : Ocepr_1015 Ocepr_2273
GOG : C1280_17980 C1280_26295 C1280_34335(thyX)
BBS : BbiDN127_A0070(thyX)
BDU : BDU_1078 BDU_16004(thyl)
BRE : BRE_1048 BRE_3005(thy1)
TPAS : TPSea814_001007(thyX)
PPIO : CE91St28_14280 CE91St28_19850
PROC : Ptc2401_01929(thyX)
DPB : BABL1_gene_161(thyX)
HARC : HARCEL1_07165(thyX)
LOB : NEF87_001658 NEF87_002736
» show all
Taxonomy
Reference
Authors
Myllykallio H, Lipowski G, Leduc D, Filee J, Forterre P, Liebl U.
Title
An alternative flavin-dependent mechanism for thymidylate synthesis.
Journal
Sequence
Reference
Authors
Griffin J, Roshick C, Iliffe-Lee E, McClarty G
Title
Catalytic mechanism of Chlamydia trachomatis flavin-dependent thymidylate synthase.
Journal
Reference
Authors
Graziani S, Bernauer J, Skouloubris S, Graille M, Zhou CZ, Marchand C, Decottignies P, van Tilbeurgh H, Myllykallio H, Liebl U
Title
Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX.
Journal
Reference
Authors
Koehn EM, Fleischmann T, Conrad JA, Palfey BA, Lesley SA, Mathews II, Kohen A
Title
An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene.
Journal
Sequence
Reference
Authors
Koehn EM, Kohen A
Title
Flavin-dependent thymidylate synthase: a novel pathway towards thymine.
Journal
Reference
Authors
Mishanina TV, Yu L, Karunaratne K, Mondal D, Corcoran JM, Choi MA, Kohen A
Title
An unprecedented mechanism of nucleotide methylation in organisms containing thyX.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 2.1.1.148