KEGG ENZYME: 2.1.1.179

ENZYME: 2.1.1.179

Entry

EC 2.1.1.179                Enzyme

Name

16S rRNA (guanine1405-N7)-methyltransferase;
methyltransferase Sgm;
m7G1405 Mtase;
Sgm Mtase;
Sgm;
sisomicin-gentamicin methyltransferase;
sisomicin-gentamicin methylase;
GrmA;
RmtB;
RmtC;
ArmA

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:16S rRNA (guanine1405-N7)-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + guanine1405 in 16S rRNA = S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA

Substrate

S-adenosyl-L-methionine [CPD:C00019];
guanine1405 in 16S rRNA

Product

S-adenosyl-L-homocysteine [CPD:C00021];
N7-methylguanine1405 in 16S rRNA

Comment

The enzyme from the antibiotic-producing bacterium Micromonospora zionensis specifically methylates guanine1405 at N7 in 16S rRNA, thereby rendering the ribosome resistant to 4,6-disubstituted deoxystreptamine aminoglycosides, which include gentamicins and kanamycins [2].

History

EC 2.1.1.179 created 2010

Orthology

K18845

16S rRNA (guanine(1405)-N(7))-methyltransferase

Genes

KPM:	KPHS_p300550

KPJ:

N559_5243

KPX:	PMK1_ndm00227(rmtB)

KOE:	A225_NDM1p0335 A225_R1p0460 A225_R1p0745

CSED:

JY391_23235

CIE:	AN232_27610(rmt) AN232_29300

PRG:	RB151_017530(rmtB)

PRQ:	CYG50_00605

PVC:	G3341_20190(rmtC)

PJU:	L1P09_25380

ABZ:	ABZJ_05040

ABR:	ABTJ_01332

ABH:

M3Q_2657

ABJ:	BJAB07104_p0094

ABAJ:

BJAB0868_p0105

ABW:	BL01_18365

CTH:

Cthe_3180

CTX:	Clo1313_0731

SKA:	CP970_06705

FRA:	Francci3_3372 Francci3_3382

KAL:

KALB_7876

KUT:	JJ691_87580(grm)

MSAG:

GCM10017556_22580 GCM10017556_22630

AFS:

AFR_30330

DVC:	Dvina_05370

DMAT:

Dmats_04960

NHY:	JQS43_21645 JQS43_21650

PSEU:

Pse7367_1027 Pse7367_1031

RRS:	RoseRS_1981

RCA:

Rcas_3259

CAU:

Caur_2845

CHL:	Chy400_3082

CAG:

Cagg_1115

ABAT:

CFX1CAM_1009 CFX1CAM_1010

PSUB:

Pelsub_P2614 Pelsub_P2615

ABAO:

BEQ56_02150

PBF:	CFX0092_A2765 CFX0092_A2776

KBS:	EPA93_27740

NEQ:

NEQ545

NAA:

Nps_01100

ARG:	QT11_C0001G0600

» show all

Reference

1 [PMID:20194115]

Authors

Husain N, Tkaczuk KL, Tulsidas SR, Kaminska KH, Cubrilo S, Maravic-Vlahovicek G, Bujnicki JM, Sivaraman J

Title

Structural basis for the methylation of G1405 in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m7G methyltransferases.

Journal

Nucleic Acids Res 38:4120-32 (2010)
DOI:10.1093/nar/gkq122

Reference

2 [PMID:19589804]

Authors

Savic M, Lovric J, Tomic TI, Vasiljevic B, Conn GL

Title

Determination of the target nucleosides for members of two families of 16S rRNA methyltransferases that confer resistance to partially overlapping groups of aminoglycoside antibiotics.

Journal

Nucleic Acids Res 37:5420-31 (2009)
DOI:10.1093/nar/gkp575

Sequence

[fra:Francci3_3372] [ag:AAA25336]

Reference

3 [PMID:18930134]

Authors

Tomic TI, Moric I, Conn GL, Vasiljevic B

Title

Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site.

Journal

Res Microbiol 159:658-62 (2008)
DOI:10.1016/j.resmic.2008.09.006

Reference

4 [PMID:18586937]

Authors

Savic M, Ilic-Tomic T, Macmaster R, Vasiljevic B, Conn GL

Title

Critical residues for cofactor binding and catalytic activity in the aminoglycoside resistance methyltransferase Sgm.

Journal

J Bacteriol 190:5855-61 (2008)
DOI:10.1128/JB.00076-08

Reference

5 [PMID:18343347]

Authors

Maravic Vlahovicek G, Cubrilo S, Tkaczuk KL, Bujnicki JM

Title

Modeling and experimental analyses reveal a two-domain structure and amino acids important for the activity of aminoglycoside resistance methyltransferase Sgm.

Journal

Biochim Biophys Acta 1784:582-90 (2008)
DOI:10.1016/j.bbapap.2007.09.009

Reference

6 [PMID:1447159]

Authors

Kojic M, Topisirovic L, Vasiljevic B

Title

Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis.

Journal

J Bacteriol 174:7868-72 (1992)
DOI:10.1128/JB.174.23.7868-7872.1992

Reference

7 [PMID:19303884]

Authors

Schmitt E, Galimand M, Panvert M, Courvalin P, Mechulam Y

Title

Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases.

Journal

J Mol Biol 388:570-82 (2009)
DOI:10.1016/j.jmb.2009.03.034

Reference

8 [PMID:20722735]

Authors

Wachino J, Shibayama K, Kimura K, Yamane K, Suzuki S, Arakawa Y

Title

RmtC introduces G1405 methylation in 16S rRNA and confers high-level aminoglycoside resistance on Gram-positive microorganisms.

Journal

FEMS Microbiol Lett 311:56-60 (2010)
DOI:10.1111/j.1574-6968.2010.02068.x

Sequence

[ag:BAE48305]

Reference

9 [PMID:16626740]

Authors

Liou GF, Yoshizawa S, Courvalin P, Galimand M

Title

Aminoglycoside resistance by ArmA-mediated ribosomal 16S methylation in human bacterial pathogens.

Journal

J Mol Biol 359:358-64 (2006)
DOI:10.1016/j.jmb.2006.03.038

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.179

IUBMB Enzyme Nomenclature:

2.1.1.179

ExPASy - ENZYME nomenclature database:

2.1.1.179

BRENDA, the Enzyme Database:

2.1.1.179

DBGET integrated database retrieval system