KEGG ENZYME: 2.1.1.180

ENZYME: 2.1.1.180

Entry

EC 2.1.1.180                Enzyme

Name

16S rRNA (adenine1408-N1)-methyltransferase;
kanamycin-apramycin resistance methylase;
16S rRNA:m1A1408 methyltransferase;
KamB;
NpmA;
16S rRNA m1A1408 methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:16S rRNA (adenine1408-N1)-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + adenine1408 in 16S rRNA = S-adenosyl-L-homocysteine + N1-methyladenine1408 in 16S rRNA

Substrate

S-adenosyl-L-methionine [CPD:C00019];
adenine1408 in 16S rRNA

Product

S-adenosyl-L-homocysteine [CPD:C00021];
N1-methyladenine1408 in 16S rRNA

Comment

The enzyme provides a panaminoglycoside-resistant nature through interference with the binding of aminoglycosides toward the A site of 16S rRNA through N1-methylation at position adenine1408 [4].

History

EC 2.1.1.180 created 2010

Orthology

K18846

16S rRNA (adenine(1408)-N(1))-methyltransferase

Genes

SCL:

sce2330

SCU:	SCE1572_13735

CCRO:

CMC5_062560

ASF:

SFBM_1345

ASM:	MOUSESFB_1254(npmA)

ASO:	SFBmNL_01416

ASB:	RATSFB_1173(npmA)

HSC:	HVS_15930(npmA)

NAH:	F5544_27470

NWL:	NWFMUON74_41140

TCU:

Tcur_0890

AHM:	TL08_05840

ACTI:

UA75_05815

ACAD:

UA74_05810

AHG:	AHOG_05565(kamB)

CAI:

Caci_9046

WWE:	P147_WWE3C01G0846

» show all

Reference

1 [PMID:2441068]

Authors

Beauclerk AA, Cundliffe E

Title

Sites of action of two ribosomal RNA methylases responsible for resistance to aminoglycosides.

Journal

J Mol Biol 193:661-71 (1987)
DOI:10.1016/0022-2836(87)90349-4

Reference

2 [PMID:17495534]

Authors

Koscinski L, Feder M, Bujnicki JM

Title

Identification of a missing sequence and functionally important residues of 16S rRNA:m(1)A1408 methyltransferase KamB that causes bacterial resistance to aminoglycoside antibiotics.

Journal

Cell Cycle 6:1268-71 (2007)
DOI:10.4161/cc.6.10.4231

Reference

3 [PMID:1840536]

Authors

Holmes DJ, Drocourt D, Tiraby G, Cundliffe E

Title

Cloning of an aminoglycoside-resistance-encoding gene, kamC, from Saccharopolyspora hirsuta: comparison with kamB from Streptomyces tenebrarius.

Journal

Gene 102:19-26 (1991)
DOI:10.1016/0378-1119(91)90532-G

Reference

4 [PMID:17875999]

Authors

Wachino J, Shibayama K, Kurokawa H, Kimura K, Yamane K, Suzuki S, Shibata N, Ike Y, Arakawa Y

Title

Novel plasmid-mediated 16S rRNA m1A1408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides.

Journal

Antimicrob Agents Chemother 51:4401-9 (2007)
DOI:10.1128/AAC.00926-07

Sequence

[ag:BAF80809]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.180

IUBMB Enzyme Nomenclature:

2.1.1.180

ExPASy - ENZYME nomenclature database:

2.1.1.180

BRENDA, the Enzyme Database:

2.1.1.180

All links

Gene (19)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (18)   
Protein sequence (38)   
   UniProt (14)   
   SWISS-PROT (3)   
   PDBSTR (21)   
DNA sequence (16)   
   GenBank (8)   
   EMBL (8)   
3D Structure (1)   
   PDB (1)   
Protein domain (2)   
   InterPro (2)   
All databases (76)   

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