Entry
Name
23S rRNA (guanine748-N1)-methyltransferase;
Rlma(II);
Rlma2;
23S rRNA m1G748 methyltransferase;
RlmaII;
Rlma II;
tylosin-resistance methyltransferase RlmA(II);
TlrB;
rRNA large subunit methyltransferase II
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:23S rRNA (guanine748-N1)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + guanine748 in 23S rRNA = S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA [RN:
R07233 ]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
guanine748 in 23S rRNA
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
N1-methylguanine748 in 23S rRNA
Comment
The enzyme specifically methylates guanine748 at N1 in 23S rRNA. The methyltransferase RlmAII confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae [1].
History
EC 2.1.1.188 created 1976 as EC 2.1.1.51, part transferred 2010 to EC 2.1.1.188
Orthology
K14336 23S rRNA (guanine748-N1)-methyltransferase
Reference
Authors
Douthwaite S, Crain PF, Liu M, Poehlsgaard J
Title
The tylosin-resistance methyltransferase RlmA(II) (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748.
Journal
Sequence
Reference
Authors
Liu M, Kirpekar F, Van Wezel GP, Douthwaite S
Title
The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA.
Journal
Sequence
Reference
Authors
Lebars I, Yoshizawa S, Stenholm AR, Guittet E, Douthwaite S, Fourmy D
Title
Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII.
Journal
Reference
Authors
Lebars I, Husson C, Yoshizawa S, Douthwaite S, Fourmy D
Title
Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA(II).
Journal
Reference
Authors
Douthwaite S, Jakobsen L, Yoshizawa S, Fourmy D
Title
Interaction of the tylosin-resistance methyltransferase RlmA II at its rRNA target differs from the orthologue RlmA I.
Journal
Reference
Authors
Liu M, Douthwaite S
Title
Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 2.1.1.188