KEGG ENZYME: 2.1.1.218

ENZYME: 2.1.1.218

Entry

EC 2.1.1.218                Enzyme

Name

tRNA (adenine9-N1)-methyltransferase;
Trm10p (ambiguous);
tRNA(m1G9/m1A9)-methyltransferase;
tRNA(m1G9/m1A9)MTase;
TK0422p (gene name);
tRNA m1A9-methyltransferase;
tRNA m1A9 Mtase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:tRNA (adenine9-N1)-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + adenine9 in tRNA = S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNA

Substrate

S-adenosyl-L-methionine [CPD:C00019];
adenine9 in tRNA

Product

S-adenosyl-L-homocysteine [CPD:C00021];
N1-methyladenine9 in tRNA

Comment

The enzyme from Sulfolobus acidocaldarius specifically methylates adenine9 in tRNA [1]. The bifunctional enzyme from Thermococcus kodakaraensis also catalyses the methylation of guanine9 in tRNA (cf. EC 2.1.1.221, tRNA (guanine9-N1)-methyltransferase).

History

EC 2.1.1.218 created 2011 (EC 2.1.1.36 created 1972, part transferred 2011 to EC 2.1.1.218)

Orthology

K15566

tRNA (adenine9-N1/guanine9-N1)-methyltransferase

Genes

SCHI:

SCHIN_v1c06060

TAM:	Theam_0430

DTE:	Dester_0946

MTEE:

MTTB_10690

METZ:

METMT2_0324

MKA:

MK1026

AFU:

AF_0031

AFG:	AFULGI_00000290

APO:	Arcpr_0444

AVE:	Arcve_1658

AST:	Asulf_00271

FPL:

Ferp_2241

GAC:

GACE_1058

GAH:

GAH_01558

PFU:

PF0678

PFI:

PFC_02550

PHO:	PH0711(PH0711)

PAB:

PAB0880

PYN:

PNA2_1359

PYA:	PYCH_07940

PYS:

Py04_0749

PYC:	TQ32_04170

TKO:

TK0422

TON:

TON_0675

TGA:

TGAM_0680

TSI:

TSIB_0970

TBA:	TERMP_00715

THE:

GQS_01580

THA:

TAM4_954

THM:

CL1_2027

TLT:

OCC_05461

THS:

TES1_0839

TNU:

BD01_0565

TEU:

TEU_09285

TGY:	X802_06960

THV:	ADU37_CDS08810

TCH:	CHITON_1103

TPEP:

A0127_02185

TPIE:

A7C91_09480

TGG:	A3K92_05160

TCE:	A3L02_03975

TBS:	A3L01_04150

THH:	CDI07_04045

TSL:	A3L11_08190

TTD:	A3L14_05990

TPRF:

A3L09_06445

TRL:	A3L10_02450

TPAF:

A3L08_07670

THY:	A3L12_04930

TIC:	FH039_07735

TCQ:	TIRI35C_1075

THEM:

FPV09_01680

THEI:

K1720_08035

PPAC:

PAP_07635

APE:

APE_0498

ACJ:

ACAM_0386

IHO:

Igni_0721

IIS:

EYM_07625

IAG:

Igag_1267

HBU:

Hbut_0079

PFM:	Pyrfu_0146

PDL:	Pyrde_0625

PABI:

PABY_03830(trm10)

PARE:

PYJP_16100

STO:

STK_08530

SOH:	D1869_04710

SSO:

SSO1087

SOL:

Ssol_2061

SSOA:

SULA_2093

SSOL:

SULB_2094

SSOF:

SULC_2092

SSHI:

J5U23_02392

SCAS:

SACC_09790

SAI:

Saci_1677

SACN:

SacN8_08140

SACR:

SacRon12I_08150

SACS:

SUSAZ_07945

SIS:	LS215_1235

SIA:	M1425_1138

SIM:	M1627_1202

SID:

M164_1128

SIY:	YG5714_1134

SIN:	YN1551_1724

SII:

LD85_1255

SIH:

SiH_1099

SIR:

SiRe_1012

SIC:

SiL_1021

SULA:

BFU36_01095

SULE:

GFS03_06515

SULO:

GFS33_11925

SULL:

EWF20_05755

MSE:

Msed_1469

MEMJ:

MJ1HA_1683

MCN:

Mcup_0756

MHK:	DFR87_11375

MPRU:

DFR88_10965

MTEN:

GWK48_00910

MJN:	MjAS7_2563

AHO:

Ahos_0922

AMAN:

B6F84_08465

ABRI:

DFR85_11875

ASUL:

DFR86_07475

AAMB:

D1866_12520

ACIH:

HS5_22990

SAZO:

D1868_10880

CSTY:

KN1_14840

STEP:

IC006_0815

SMET:

RQ359_001013(trm10)

PAI:

PAE0001

PIS:

Pisl_0031

PCL:

Pcal_0647

PAS:

Pars_1096

PYR:

P186_0834

POG:

Pogu_1177

TNE:

Tneu_0946

PYW:	PYWP30_01088

CMA:

Cmaq_1633

TTN:

TTX_0355

TUZ:

TUZN_0832

VDI:

Vdis_0727

VMO:

VMUT_1574

VSO:	Vsou_13860

ASC:

ASAC_0477

ACIA:

SE86_03265

CLG:	Calag_0035

NEQ:

NEQ005

NAA:

Nps_02650

» show all

Reference

1 [PMID:20525789]

Authors

Kempenaers M, Roovers M, Oudjama Y, Tkaczuk KL, Bujnicki JM, Droogmans L

Title

New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA.

Journal

Nucleic Acids Res 38:6533-43 (2010)
DOI:10.1093/nar/gkq451

Sequence

[sai:Saci_1677] [tko:TK0422]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.218

IUBMB Enzyme Nomenclature:

2.1.1.218

ExPASy - ENZYME nomenclature database:

2.1.1.218

BRENDA, the Enzyme Database:

2.1.1.218

DBGET integrated database retrieval system