KEGG ENZYME: 2.1.1.258

ENZYME: 2.1.1.258

Entry

EC 2.1.1.258                Enzyme

Name

5-methyltetrahydrofolate---corrinoid/iron-sulfur protein Co-methyltransferase;
acsE (gene name)

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

5-methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase

Reaction(IUBMB)

a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate = a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate [RN:R02289]

Reaction(KEGG)

R02289;
(other) R10243

Substrate

[methyl-Co(III) corrinoid Fe-S protein];
tetrahydrofolate [CPD:C00101]

Product

[Co(I) corrinoid Fe-S protein];
5-methyltetrahydrofolate [CPD:C00440]

Comment

Catalyses the transfer of a methyl group from the N5 group of methyltetrahydrofolate to the 5-methoxybenzimidazolylcobamide cofactor of a corrinoid/Fe-S protein. Involved, together with EC 1.2.7.4, anaerobic carbon-monoxide dehydrogenase and EC 2.3.1.169, CO-methylating acetyl-CoA synthase, in the reductive acetyl coenzyme A (Wood-Ljungdahl) pathway of autotrophic carbon fixation in various bacteria and archaea.

History

EC 2.1.1.258 created 2012

Pathway

ec00720

Carbon fixation pathways in prokaryotes

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K15023

5-methyltetrahydrofolate corrinoid/iron sulfur protein methyltransferase

Genes

XOR:

XOC_1783

XOZ:	BE73_08365

ABRE:

pbN1_19000

BVY:	NCTC9239_01829(metH_2)

DSF:

UWK_01140

DLM:	DPPLL_26570

DAK:	DaAHT2_1991 DaAHT2_2486

DBK:	DGMP_17860

DDU:

GF1_31690

ECOM:

QTN59_15335

DOL:	Dole_2576 Dole_2853 Dole_3182

DAL:	Dalk_0683 Dalk_4619 Dalk_4910

DAT:	HRM2_00520(metH1) HRM2_16640(metH3)

DTO:	TOL2_C05310 TOL2_C11790(metH)

DOV:	DSCO28_10640 DSCO28_24780 DSCO28_59710

DWD:	DSCW_06570 DSCW_19000 DSCW_28550 DSCW_34490

DALK:

DSCA_20770 DSCA_26740 DSCA_44750 DSCA_55930 DSCA_62150

DML:	Dmul_15220 Dmul_30880(metH) Dmul_32250

DLI:	dnl_12310 dnl_32590 dnl_60060

DMM:	dnm_013350 dnm_058130

DEK:	DSLASN_16780

DAO:	Desac_0369 Desac_1969

DTI:	Desti_0229 Desti_0441 Desti_0981 Desti_2096 Desti_2349 Desti_4769

SFU:

Sfum_2563

DAX:	FDQ92_06230

DBR:	Deba_0238 Deba_1009 Deba_1649

DMP:	FAK_22470 FAK_41990

TID:	Thein_1435 Thein_2179

TCM:	HL41_07615

THET:

F1847_08565

CTHI:

THC_1765

TAV:	G4V39_01645 G4V39_10610

TMAI:

FVE67_00220

DHR:	LGS26_01905

CLJ:	CLJU_c07500(metH2) CLJU_c07550(metH3) CLJU_c20620(metH4) CLJU_c20690(metH5) CLJU_c37560(meTr)

CLB:	Clo1100_0739

CAH:	CAETHG_0145 CAETHG_1609(acsE) CAETHG_2843 CAETHG_2848

CSQ:	CSCA_0288 CSCA_4550 CSCA_5050

CACE:

CACET_c10310(acsE) CACET_c12990 CACET_c32510(metH2)

CCK:	Ccar_13140 Ccar_15510 Ccar_18790

CFM:	BJL90_01660 BJL90_02620 BJL90_11130 BJL90_20245

CDRK:

B9W14_17025 B9W14_19560 B9W14_22255

CLOS:

DMR38_03585 DMR38_03610 DMR38_03630 DMR38_10555 DMR38_10590 DMR38_18725

CCE:

Ccel_2473

CCEL:

CCDG5_0495

RUM:	CK1_05540 CK1_12640 CK1_29210

RGV:	NQ502_01485(acsE)

DSY:	DSY0589 DSY1268 DSY1647 DSY2719 DSY4702 DSY4754 DSY4972

DHD:	Dhaf_0540 Dhaf_0722 Dhaf_1108 Dhaf_1838 Dhaf_1896 Dhaf_2367 Dhaf_2568 Dhaf_2792 Dhaf_3883 Dhaf_4612 Dhaf_4640 Dhaf_4871

DDH:	Desde_2049 Desde_2250 Desde_3331 Desde_3948

DDL:	Desdi_1916 Desdi_2542 Desdi_2551 Desdi_3330

DMT:	DESME_07355

DOR:	Desor_1138 Desor_1147 Desor_1650 Desor_2083 Desor_2369 Desor_2613 Desor_2716 Desor_2734 Desor_2799 Desor_2943 Desor_3268 Desor_4150 Desor_4258 Desor_4397 Desor_5170

DAI:	Desaci_2790 Desaci_2831 Desaci_2969

DMI:	Desmer_1620 Desmer_2202 Desmer_2353 Desmer_2530 Desmer_3193 Desmer_3491

DED:	DHBDCA_p1157 DHBDCA_p2017 DHBDCA_p225 DHBDCA_p232 DHBDCA_p2404

DEC:	DCF50_p1224 DCF50_p2029 DCF50_p2416 DCF50_p285 DCF50_p292

DRS:	DEHRE_00710 DEHRE_00745 DEHRE_03815 DEHRE_04045 DEHRE_06900

PTH:	PTH_1004(MetH)

DAE:

Dtox_1276

DKU:	Desku_1487 Desku_2712

DAU:	Daud_0113 Daud_0876

FWA:	DCMF_02510 DCMF_05840 DCMF_07395 DCMF_07885 DCMF_08110 DCMF_08795 DCMF_12955 DCMF_16370 DCMF_23120 DCMF_26780 DCMF_27825 DCMF_27850 DCMF_28165 DCMF_28355

AACX:

DEACI_2516 DEACI_4249

TJR:	TherJR_1161 TherJR_2629

DGI:	Desgi_0674 Desgi_2047 Desgi_3921

HCV:	FTV88_3024(metH)

ELM:	ELI_0487 ELI_0560 ELI_2005 ELI_3604 ELI_3713

EMT:	CPZ25_002655 CPZ25_011395 CPZ25_015290 CPZ25_018700

ELIM:

B2M23_01055 B2M23_07880 B2M23_08395 B2M23_16860

AWO:	Awo_c05180(mttA1) Awo_c09210(mttA3) Awo_c10730(acsE) Awo_c15660(mttA4) Awo_c18280(mttA5) Awo_c22740 Awo_c23260(mttB19) Awo_c23750(mttA8)

AWI:	CHL1_000721 CHL1_001163 CHL1_001288 CHL1_001470 CHL1_002433 CHL1_002704 CHL1_002854 CHL1_003243

ALKA:

J0B03_02805 J0B03_03050 J0B03_10100 J0B03_11260 J0B03_11305

CTHM:

CFE_1661

ROB:

CK5_28650

BYL:	A4V09_05475 A4V09_19575

BHAN:

CGC63_07955

BLAU:

DQQ01_01790 DQQ01_09710

BPRO:

PMF13cell1_00337(acsE_1) PMF13cell1_01636(acsE_2) PMF13cell1_03817(acsE_3) PMF13cell1_04280(acsE_4)

BLAB:

EYS05_14255 EYS05_16710

BLIQ:

INP51_05970

BWX:	NQ550_13495(acsE)

BMAS:

LK422_06270

BLAR:

LC508_02780 LC508_16895

BLUI:

Blut17040_19180(acsE)

BCOC:

BLCOC_27960(acsE_2) BLCOC_33080(acsE_3)

BHV:	BLHYD_15440(acsE)

QDO:	H9Q78_01455

WHJ:	H9Q79_03170 H9Q79_06470

MFOR:

NQ534_19165(acsE)

AMT:	Amet_2027 Amet_2085 Amet_2092

GFE:	Gferi_04565 Gferi_06320 Gferi_07815 Gferi_15430 Gferi_16345 Gferi_16365 Gferi_17595 Gferi_17730

PBIF:

KXZ80_13905

PSOR:

RSJ16_15715

CDF:	CD630_07270

PDC:	CDIF630_00843(acsE)

CDC:	CD196_0673

CDL:	CDR20291_0654

PDF:	CD630DERM_07270

ROC:	HF520_08190 HF520_11265

TEM:	JW646_10890

TGC:	L0P85_16870(acsE)

TMY:	TEMA_26060(acsE)

TEB:

T8CH_0660

THYR:

P4S50_13875(acsE)

IBR:	NQ514_13090(acsE)

CST:	CLOST_1170

TKI:	TKV_c19830(acsE)

CHY:	CHY_1227(acsE)

ADG:

Adeg_0341

TPZ:	Tph_c15130(acsE) Tph_c27600(metH3)

AAUT:

ACETAC_10540

MAS:	Mahau_1083

MTA:

Moth_1197

MTHO:

MOTHE_c03140(acsE1) MOTHE_c11730(acsE2)

MTHZ:

MOTHA_c12600(acsE2)

TEP:	TepRe1_0302 TepRe1_0560

TAE:	TepiRe1_0337(acsE) TepiRe1_0612

BACG:

D2962_12360

TOC:	Toce_0796 Toce_0812 Toce_1080

NCD:	ACONDI_02974(acsE_5)

AAR:	Acear_0930 Acear_0954 Acear_1056 Acear_1299

KME:	H0A61_00825(acsE)

CSC:

Csac_0898

ATE:

Athe_0749

COB:	COB47_0680

CHD:	Calhy_1941

COW:	Calow_0596

CLC:	Calla_0391

CKN:	Calkro_1902

MANA:

MAMMFC1_00471(acsE_1) MAMMFC1_01778(acsE_2) MAMMFC1_02498(acsE_3)

STED:

SPTER_03920(acsE_1) SPTER_09890(acsE_2) SPTER_13730(acsE_3) SPTER_28050(acsE_4) SPTER_30460(acsE_6)

CALK:

HUE98_15605

DLY:	Dehly_0819

DEW:	DGWBC_1388

OTE:

Oter_4334

TTF:

THTE_3701

KST:	KSMBR1_1319(acsE)

BROC:

IPI25_11050

BPIT:

BPIT_02180

JET:	L3J17_07120

PBU:	L21SP3_00049(acsE)

PBP:	STSP1_00914(metH_1)

ALUS:

STSP2_01509(acsE)

VBL:	L21SP4_02015(acsE_1) L21SP4_02433(acsE_2)

VAI:	BU251_04945

TPI:	TREPR_1556 TREPR_2898 TREPR_3064

SSM:	Spirs_2845

TAZ:	TREAZ_1644 TREAZ_1652

RAG:

B739_0062

RAT:

M949_0493

TYE:	THEYE_A1473

DTP:	JZK55_05450 JZK55_20280

MER:	MMINT_00400

» show all

Reference

1 [PMID:7928975]

Authors

Roberts DL, Zhao S, Doukov T, Ragsdale SW

Title

The reductive acetyl coenzyme A pathway: sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum.

Journal

J Bacteriol 176:6127-30 (1994)
DOI:10.1128/JB.176.19.6127-6130.1994

Sequence

[mta:Moth_1197]

Reference

2 [PMID:10997901]

Authors

Doukov T, Seravalli J, Stezowski JJ, Ragsdale SW

Title

Crystal structure of a methyltetrahydrofolate- and corrinoid-dependent methyltransferase.

Journal

Structure 8:817-30 (2000)
DOI:10.1016/S0969-2126(00)00172-6

Sequence

[mta:Moth_1197]

Reference

3 [PMID:17172470]

Authors

Doukov TI, Hemmi H, Drennan CL, Ragsdale SW

Title

Structural and kinetic evidence for an extended hydrogen-bonding network in catalysis of methyl group transfer. Role of an active site asparagine residue in activation of methyl transfer by methyltransferases.

Journal

J Biol Chem 282:6609-18 (2007)
DOI:10.1074/jbc.M609828200

Sequence

[mta:Moth_1197]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.258

IUBMB Enzyme Nomenclature:

2.1.1.258

ExPASy - ENZYME nomenclature database:

2.1.1.258

BRENDA, the Enzyme Database:

2.1.1.258

DBGET integrated database retrieval system