Entry
Name
flavonoid 3',5'-methyltransferase;
AOMT;
CrOMT2
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:flavonoid 3'-O-methyltransferase
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + a 3'-hydroxyflavonoid = S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid;
(2) S-adenosyl-L-methionine + a 5'-hydroxy-3'-methoxyflavonoid = S-adenosyl-L-homocysteine + a 3',5'-dimethoxyflavonoid
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
3'-hydroxyflavonoid [CPD:
C02790 ];
5'-hydroxy-3'-methoxyflavonoid
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
3'-methoxyflavonoid;
3',5'-dimethoxyflavonoid
Comment
Isolated from Vitis vinifera (grape) [2]. Most active with delphinidin 3-glucoside but also acts on cyanidin 3-glucoside, cyanidin, myricetin, quercetin and quercetin 3-glucoside. The enzyme from Catharanthus roseus was most active with myricetin [1].
History
EC 2.1.1.267 created 2013, modified 2014
Pathway
ec00944 Flavone and flavonol biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology
K13272 flavonoid O-methyltransferase
Genes
HSYR : 120138721 120197908
PMUM : 103322058 103331367
PAVI : 110756049 110769740
PDUL : 117616544 117619928
MSYL : 126595165 126611729
CSAV : 115712603 115712971
QSU : 112011645 112011654 112017664 112017707
VVI : 100233134(FAOMT) 100245452 100250567 100250579
VRI : 117911684 117922688 117923246
SIND : 105156703 105168404 105168406 105168407
OEU : 111391869 111393471 111393476
EGT : 105950082 105952615 105952640 105958570 105958578 105958584 105970959
SSPL : 121793763 121798458 121802564 121809514
SMIL : 130995613 130995615
SHIS : 125198982 125200483 125204768 125211759 125211845 125217009
APAN : 127241181 127241183 127244939
CSIN : 114275659 114293531 114305583 114312686 114317032
RVL : 131311302 131311303 131311304
ATRI : 130809233 130809234
TSS : 122647755 122652785 122652787 122663698
» show all
Taxonomy
Reference
Authors
Cacace S, Schroder G, Wehinger E, Strack D, Schmidt J, Schroder J
Title
A flavonol O-methyltransferase from Catharanthus roseus performing two sequential methylations.
Journal
Sequence
Reference
Authors
Hugueney P, Provenzano S, Verries C, Ferrandino A, Meudec E, Batelli G, Merdinoglu D, Cheynier V, Schubert A, Ageorges A
Title
A novel cation-dependent O-methyltransferase involved in anthocyanin methylation in grapevine.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.1.1.267