KEGG ENZYME: 2.1.1.98

ENZYME: 2.1.1.98

Entry

EC 2.1.1.98                 Enzyme

Name

diphthine synthase;
S-adenosyl-L-methionine:elongation factor 2 methyltransferase (ambiguous);
diphthine methyltransferase (ambiguous);
S-adenosyl-L-methionine:2-(3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] methyltransferase;
Dph5 (ambiguous)

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] methyltransferase (diphthine-[translation elongation factor 2]-forming)

Reaction(IUBMB)

3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] (overall reaction) [RN:R10306];
(1a) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] [RN:R04481];
(1b) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] [RN:R08468];
(1c) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] [RN:R08469]

Reaction(KEGG)

R04481 R08468 R08469 R10306

Substrate

S-adenosyl-L-methionine [CPD:C00019];
2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] [CPD:C04441];
2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C04692];
2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C16720]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
diphthine-[translation elongation factor 2] [CPD:C01573];
2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C04692];
2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C16720]

Comment

This archaeal enzyme produces the trimethylated product diphthine, which is converted into diphthamide by EC 6.3.1.14, diphthine---ammonia ligase. Different from the eukaryotic enzyme, which produces diphthine methyl ester (cf. EC 2.1.1.314). In the archaeon Pyrococcus horikoshii the enzyme acts on His600 of elongation factor 2.

History

EC 2.1.1.98 created 1990, modified 2013, modified 2015

Orthology

K20215

diphthine synthase

Genes

MANI:

DP067_03580

MJA:

MJ_1274

MFE:	Mefer_0044

MVU:	Metvu_1279

MFS:	MFS40622_0657

MIF:	Metin_0832

MJH:	JH146_0451

MESG:

MLAUSG7_0028(dphB)

MESA:

MLASG1_1208(dphB)

MIG:	Metig_1174

MMP:	MMP0588(dph5)

MMQ:	MmarC5_1019

MMX:	MmarC6_0305

MMZ:	MmarC7_1609

MMD:

GYY_03540

MMAK:

MMKA1_06360

MMAO:

MMOS7_06310

MMAD:

MMJJ_03970

MAE:

Maeo_0435

MVN:	Mevan_1459

MVO:

Mvol_0810

MOK:	Metok_0033

METF:

CFE53_00595

MTH:

MTH_1874

MMG:	MTBMA_c04450(dphB)

MWO:	MWSIV6_0405(dphB)

MTEE:

MTTB_11810

METC:

MTCT_1712

METE:

tca_01810

METZ:

METMT2_0209

METK:

FVF72_02335(dph5)

MTHM:

FZP57_01900(dph5)

METJ:

FZP68_06930(dph5)

MST:	Msp_1017(dphB)

METB:

AW729_10810

MEIS:

PXD04_02595(dph5)

MRU:	mru_1764(dphB)

MSI:

Msm_0801

MEB:	Abm4_1315(dphB)

MMIL:

sm9_1721(dphB)

MEYE:

TL18_07670

MOL:

YLM1_1219

MARO:

MarbSA_07220

METV:

K4897_07490(dph5)

MEL:	Metbo_0693

MEW:	MSWAN_1562

METH:

MBMB1_1281(dphB)

MFC:	BRM9_0500(dphB)

MFI:	DSM1535_0480(dphB)

MCUB:

MCBB_1499(dphB)

MSUB:

BK009_05330

METN:

BK008_00875

METT:

CIT01_08420

METO:

CIT02_11640

MEME:

HYG87_08875

MFEG:

GCM10025860_26300

MFV:

Mfer_0700

MKA:	MK0747(dph5)

AFU:

AF_0381

AFG:	AFULGI_00003810

APO:	Arcpr_0161

AVE:	Arcve_1504

AST:	Asulf_00669

FPL:

Ferp_1481

GAC:

GACE_1631

GAH:

GAH_00943

PFU:

PF0595

PFI:

PFC_02105

PHO:	PH0725(PH0725)

PAB:

PAB1501

PYN:

PNA2_1369

PYA:	PYCH_07540

PYS:

Py04_0739

PYC:	TQ32_04245

TKO:

TK0106

TON:

TON_1372

TGA:	TGAM_0314(dph5)

TSI:

TSIB_0124

TBA:	TERMP_01321

THE:

GQS_08695

THA:

TAM4_1375

THM:

CL1_1363

TLT:

OCC_06971

THS:

TES1_1349

TNU:

BD01_2145

TEU:

TEU_05045

TGY:	X802_05025

THV:	ADU37_CDS00450

TCH:	CHITON_1118

TPEP:

A0127_03225

TPIE:

A7C91_02410

TGG:	A3K92_07750

TCE:	A3L02_04310

TBS:	A3L01_07595

THH:	CDI07_07985

TSL:	A3L11_00445

TTD:	A3L14_09950

TPRF:

A3L09_00145

TRL:	A3L10_06995

TPAF:

A3L08_00780

THY:	A3L12_02145

TIC:	FH039_10525

TCQ:	TIRI35C_1840(dphB)

THEM:

FPV09_10635

THEI:

K1720_03710(dph5)

PPAC:

PAP_06625

MBA:	Mbar_A2900

MBY:	MSBRM_1431

MBW:	MSBRW_1838

MBAR:

MSBR2_0974

MBAK:

MSBR3_0271

MAC:	MA_1370(dph5)

MMA:

MM_2355

MMAZ:

MmTuc01_2405

MMJ:	MSMAS_2344

MMAC:

MSMAC_2101

MVC:	MSVAZ_1780

MEK:	MSKOL_1756

MLS:	MSLAZ_0954

METM:

MSMTP_2166

MEF:	MSWH1_2299

MEQ:	MSWHS_2776

MSJ:	MSSAC_1376

MSZ:	MSSIH_1580

MSW:	MSSIT_1666

MTHR:

MSTHT_0849

MTHE:

MSTHC_2422

MHOR:

MSHOH_2966

MFZ:	AOB57_012250

MBU:

Mbur_0971

MMET:

MCMEM_1474

MELO:

J7W08_06130

MMH:

Mmah_0645

MHAZ:

BHR79_00140

MPOT:

BKM01_08275

MEV:	Metev_1417

MZH:	Mzhil_0765

MPY:

Mpsy_3166

MZI:	HWN40_07825

MMAV:

RE476_01870(dph5)

MSEB:

RE474_11640(dph5)

MHZ:	Metho_1698

MEHF:

MmiHf6_02980

MEES:

MmiEs2_05950

MTP:

Mthe_1120

MCJ:	MCON_1449(dphB)

MHI:

Mhar_0531

MHU:

Mhun_0788

MRTJ:

KHC33_09675(dph5)

MLA:

Mlab_0386

MEM:	Memar_1783

MBG:	BN140_0607(dphB)

MEMA:

MMAB1_0789(dphB)

MCHK:

MchiMG62_20420

MSUM:

OH143_03130(dph5)

MRC:	R6Y96_08565(dph5)

MPI:

Mpet_0734

MEND:

L6E24_04935(dph5)

MEFW:

F1737_07390

MAQE:

RJ40_09745

MFK:	E2N92_09965

MOU:	OU421_12935(dph5)

MESB:

L1S32_04690(dph5)

MANQ:

L1994_07610(dph5)

MBN:

Mboo_2068

MFO:	Metfor_0785

MPL:

Mpal_0116

MPD:	MCP_0924(dphB)

MEZ:

Mtc_2082

RCI:	RRC519(dphB)

HAL:	VNG_1118G(lds)

HSL:	OE_2613R(dph5)

HANR:

LJ422_04565(dph5)

HDL:	HALDL1_10650

HHB:	Hhub_2319(dph5)

HAGS:

JT689_05820

HABO:

JRZ79_04285(dph5)

HNO:	LT974_07305(dph5)

HLU:	LT972_06360(dph5)

HALH:

HTSR_1064(dph5)

HHSR:

HSR6_1101(dph5)

HSU:	HLASF_1105

HSF:	HLASA_1094

HAHS:

HSRCO_1614(dPH5)

SALR:

FQU85_10295

HALR:

EFA46_008765

HJE:	HacjB3_08510

HDO:	MUK72_06720(dph5)

HALX:

M0R89_11905(dph5)

HAXZ:

M0R88_12170(dph5)

HAYC:

NGM10_02475(dph5)

HAXY:

NGM07_00890(dph5)

SALI:

L593_14760

SAIM:

K0C01_02330(dph5)

HARA:

AArcS_0737(dph5)

HMA:	rrnAC1406(dph5)

HHI:	HAH_1989(dph5)

HHN:	HISP_10135

HAB:	SG26_02425

HTA:	BVU17_09690

HALJ:

G9465_14495

HSIN:

KDQ40_03505

NPH:	NP_1884A(dph5)

NMO:	Nmlp_1959(dph5)

NHO:	HWV23_04205

NSAL:

HWV07_03245

HUT:

Huta_0243

HTI:

HTIA_0426

HALA:

Hrd1104_10395

HASV:

SVXHr_1339(dph5)

HABN:

HBNXHr_1324(dph5)

HMU:

Hmuk_1953

HALZ:

E5139_12875

HALL:

LC1Hm_1275(dph5)

HAZP:

GBQ70_12890

HALI:

BV210_14315

HSN:	DV733_02505

HDS:	HSR122_2165(dph5)

HRR:	HZS55_11000

HPEL:

HZS54_15390

HLT:	I7X12_03015

HARC:

HARCEL1_11525(dph5)

SSAI:

N0B31_02235(dph5)

HAAD:

MW046_05470(dph5)

HWA:	HQ_1603A(dph5)

HWC:	Hqrw_1714(dph5)

HVO:	HVO_0916(dph5)

HME:	HFX_0887(dph5)

HGI:	ABY42_04405

HALE:

G3A49_04580

HLS:	KU306_09200(dph5)

HLN:	SVXHx_0870(dph5)

HBO:	Hbor_23080

HAQ:	DU484_14240

HAJ:	DU500_14270

HAER:

DU502_12385

HRA:	EI982_17120

HLM:	DV707_10280

HALM:

FCF25_11605

HLA:

Hlac_0892

HALP:

DOS48_03675

HALB:

EKH57_07480

HEZZ:

EO776_04900

HALQ:

Hrr1229_002320

HSS:	J7656_11935

HSAI:

HPS36_02260

HAZZ:

KI388_02455(dph5)

SRUB:

C2R22_05630

HAE:	halTADL_1046

HALN:

B4589_000415

HALG:

HUG10_14700

HALU:

HUG12_01595

HMP:	K6T50_10855(dph5)

HRE:	K6T36_10640(dph5)

HRM:	K6T25_05420(dph5)

HACB:

Hbl1158_02650(dph5)

HDF:	AArcSl_0117(dph5)

HAAA:

AArcCO_0260(dPH5)

HAH:	Halar_0827

HTU:

Htur_2752

HSAL:

JMJ58_14720

HAKZ:

J0X25_01980(dph5)

NMG:	Nmag_0897(dph5)

HXA:	Halxa_2402

NAT:

NJ7G_1756

NPE:	Natpe_0971

NVR:	FEJ81_05005

NPL:	FGF80_05800

NAY:	HYG81_06255(dph5)

HALY:

HYG82_08855

HJT:	DVR14_14165

HLO:	J0X27_11790

NGE:	Natgr_0089

HRU:	Halru_1978

SAWL:

NGM29_07645(dph5)

HALV:

NGM15_00130(dph5)

NOU:	Natoc_2717

HLR:	HALLA_08355

HLC:	CHINAEXTREME08855

NAJ:	B1756_01955

NAG:	AArcMg_0796

NAN:	AArc1_2709

NARA:

QQ977_00435(dph5)

NBG:	DV706_10350

NAS:	GCU68_01725

HDA:	BB347_09440

NAC:	AQV86_05655

MELU:

MTLP_09690

MEAM:

MU439_07250(dph5)

MEAE:

QEN48_02970(dph5)

TAC:

Ta0883

TVO:	TVG1034064(TVG1034064)

PTO:

PTO1233

FAC:	FACI_IFERC01G1335

FAI:

FAD_0348

CDIV:

CPM_1291

TAR:	TALC_01129

MAX:	MMALV_11700

MER:	MMINT_14280

MEAR:

Mpt1_c02170(dphB)

MEUZ:

KRP56_05995(dph5)

MARC:

AR505_1284

ABI:

Aboo_0591

ACF:	AciM339_0455

APE:	APE_0931(dphB)

ACJ:	ACAM_0608(dphB)

SMR:

Smar_0509

SHC:	Shell_0288

IHO:

Igni_0657

IIS:

EYM_06210

DKA:

DKAM_1445

DFD:	Desfe_0069

DMU:	Desmu_0139

TAG:

Tagg_1079

IAG:

Igag_0048

THG:	TCELL_0750

HBU:

Hbut_0128

PFM:	Pyrfu_1089

PDL:	Pyrde_0677

PABI:

PABY_04360(dph5)

PARE:

PYJP_15740(dph5)

STO:	STK_12780(dphB)

SOH:	D1869_07400

SSO:

SSO0953

SOL:

Ssol_1929

SSOA:

SULA_1961

SSOL:

SULB_1962

SSOF:

SULC_1960

SSHI:

J5U23_02525

SCAS:

SACC_08370

SAI:

Saci_1334

SACN:

SacN8_06505

SACR:

SacRon12I_06495

SACS:

SUSAZ_06375

SIS:	LS215_1360

SIA:	M1425_1273

SIM:	M1627_1323

SID:

M164_1257

SIY:	YG5714_1258

SIN:	YN1551_1590

SII:

LD85_1385

SIH:

SiH_1218

SIR:

SiRe_1136

SIC:

SiL_1133

SULA:

BFU36_01670

SULE:

GFS03_07145

SULL:

EWF20_08575

MSE:

Msed_1735

MEMJ:

MJ1HA_1980

MCN:

Mcup_0493

MHK:	DFR87_09985

MPRU:

DFR88_09560

MTEN:

GWK48_02290

MJN:	MjAS7_2863

AHO:

Ahos_1400

AMAN:

B6F84_11460

ABRI:

DFR85_10815

ASUL:

DFR86_09775

AAMB:

D1866_01935

ACIH:

HS5_28120

SACD:

HS1genome_1023

SAZO:

D1868_04320

CSTY:

KN1_24530

STEP:

IC006_1161

SMET:

RQ359_000749(dph5)

SAHS:

HS7_18650

PAI:

PAE1139

PIS:

Pisl_1283

PCL:

Pcal_0705

PAS:

Pars_0230

PYR:

P186_2537

POG:

Pogu_2233

TNE:

Tneu_0216

PYW:	PYWP30_00283

CMA:

Cmaq_1990

TTN:	TTX_0095(dph5)

TUZ:

TUZN_1249

VDI:

Vdis_2028

VMO:

VMUT_0435

TPE:

Tpen_0689

THB:	N186_04685

TCB:	TCARB_1964

THEL:

IG193_03945(dph5)

ASC:

ASAC_0414

CLG:	Calag_1209

FFO:	FFONT_1021

NMR:

Nmar_1297

NIR:	NSED_07195

NID:	NPIRD3C_0827

NIN:	NADRNF5_0659

NIW:	Nisw_07245(dph5)

NCL:	C5F47_06985(dph5)

NOX:	C5F49_06995(dph5)

NUE:	C5F50_09750(dph5)

NIP:	NsoK4_01555(dph5)

NZT:	NZOSNM25_000514

NCT:	NMSP_0391(dphB)

NIC:	DSQ20_07950(dph5)

NGA:	Ngar_c14540

NVN:	NVIE_002640

NEV:

NTE_01580

TAA:	NMY3_02230

NFN:	NFRAN_2992

NCV:

NCAV_1393

CSY:	CENSYa_0126

CSU:	CSUB_C1500

NBV:	T478_1052(dph5)

TAH:	SU86_009035

NIU:	DSQ19_03180(dph5)

NCK:	QVH35_07160(dph5)

NDV:	NDEV_1632(dph)

CCAI:

NAS2_0256

BARC:

AOA65_2396(dphB)

BARB:

AOA66_1245(dphB)

NEQ:

NEQ422

NAA:

Nps_01115

MARH:

Mia14_0002

FLT:	Sv326_0989

MIY:	Micr_00043(dphB_2)

NCON:

LC1Nh_0925(dph5)

AGW:	QT03_C0001G0336

ARG:	QT11_C0001G0598

» show all

Reference

1 [PMID:20873788]

Authors

Zhu X, Kim J, Su X, Lin H

Title

Reconstitution of diphthine synthase activity in vitro.

Journal

Biochemistry 49:9649-57 (2010)
DOI:10.1021/bi100812h

Sequence

[pho:PH0725]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.98

IUBMB Enzyme Nomenclature:

2.1.1.98

ExPASy - ENZYME nomenclature database:

2.1.1.98

BRENDA, the Enzyme Database:

2.1.1.98

CAS:	114514-25-9

DBGET integrated database retrieval system