KEGG ENZYME: 2.1.3.10

ENZYME: 2.1.3.10

Entry

EC 2.1.3.10                 Enzyme

Name

malonyl-S-ACP:biotin-protein carboxyltransferase;
malonyl-S-acyl-carrier protein:biotin-protein carboxyltransferase;
MadC/MadD;
MadC,D;
malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxyltransferase

Class

Transferases;
Transferring one-carbon groups;
Carboxy- and carbamoyltransferases

Sysname

malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxytransferase

Reaction(IUBMB)

a malonyl-[acyl-carrier protein] + a biotinyl-[protein] = an acetyl-[acyl-carrier protein] + a carboxybiotinyl-[protein] [RN:R08936]

Reaction(KEGG)

R08936

Substrate

malonyl-[acyl-carrier protein] [CPD:C01209];
biotinyl-[protein] [CPD:C18046]

Product

acetyl-[acyl-carrier protein] [CPD:C03939];
carboxybiotinyl-[protein] [CPD:C18047]

Comment

Derived from the components MadC and MadD of the anaerobic bacterium Malonomonas rubra, this enzyme is a component of EC 7.2.4.4, biotin-dependent malonate decarboxylase. The carboxy group is transferred from malonate to the cofactor of the biotin protein (MadF) with retention of configuration [2]. Similar to EC 4.1.1.87, malonyl-S-ACP decarboxylase, which forms part of the biotin-independent malonate decarboxylase (EC 4.1.1.88), this enzyme also follows on from EC 2.3.1.187, acetyl-S-ACP:malonate ACP transferase, and results in the regeneration of the acetyl-[acyl-carrier protein] [3].

History

EC 2.1.3.10 created 2008, modified 2018

Orthology

K20510

malonyl-S-ACP:biotin-protein carboxyltransferase subunit MadC

K20511

malonyl-S-ACP:biotin-protein carboxyltransferase subunit MadD

Genes

EFE:	EFER_3130 EFER_3131

KPZ:	KPNIH27_22440 KPNIH27_22445

KPB:	FH42_14170 FH42_14175

KLW:	DA718_03335(mdcE) DA718_03340

KGR:	JJJ10_02970(mdcE) JJJ10_02975

KPAS:

LUW96_03545 LUW96_03550(mdcE)

KLC:	K7H21_02980(mdcE) K7H21_02985

KLM:	BWI76_25005 BWI76_25010

CAMA:

F384_01155 F384_01160

CTEL:

GBC03_24695 GBC03_24700(mdcE)

SFO:	Z042_24845 Z042_26175

RPB:	RPB_3295 RPB_3296

RPC:	RPC_1059 RPC_1060

RCP:	RCAP_rcp00127(mdcE) RCAP_rcp00128(mdcB)

RAP:	RHOA_4940(madC) RHOA_4941(madD)

ATI:	AL072_31130 AL072_31135

AZZ:	DEW08_20130(mdcE) DEW08_20135

RRU:	Rru_A1324 Rru_A1325

RRF:	F11_06850 F11_06855

DES:	DSOUD_0385 DSOUD_0386

PFT:	JBW_01392 JBW_01393

» show all

Reference

1 [PMID:9128730]

Authors

Berg M, Hilbi H, Dimroth P

Title

Sequence of a gene cluster from Malonomonas rubra encoding components of the malonate decarboxylase Na+ pump and evidence for their function.

Journal

Eur J Biochem 245:103-15 (1997)
DOI:10.1111/j.1432-1033.1997.00103.x

Sequence

[ag:AAC45402 AAC45403]

Reference

Authors

Micklefield, J., Harris, K.J., Groger, S., Mocek, U., Hilbi, H., Dimroth, P. and Floss, H.G.

Title

Stereochemical course of malonate decarboxylase in Malonomonas rubra has biotin decarboxylation with retention.

Journal

J Am Chem Soc 117:1153-1154 (1995)

Reference

3 [PMID:11902724]

Authors

Dimroth P, Hilbi H

Title

Enzymic and genetic basis for bacterial growth on malonate.

Journal

Mol Microbiol 25:3-10 (1997)
DOI:10.1046/j.1365-2958.1997.4611824.x

Other DBs

ExplorEnz - The Enzyme Database:

2.1.3.10

IUBMB Enzyme Nomenclature:

2.1.3.10

ExPASy - ENZYME nomenclature database:

2.1.3.10

BRENDA, the Enzyme Database:

2.1.3.10

DBGET integrated database retrieval system