KEGG ENZYME: 2.4.1.155

ENZYME: 2.4.1.155

Entry

EC 2.4.1.155                Enzyme

Name

alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase;
MGAT5 (gene name);
N-acetylglucosaminyltransferase V;
alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-alpha-mannoside beta1->6-acetylglucosaminyltransferase;
UDP-N-acetylglucosamine:alpha-mannoside-beta1,6 N-acetylglucosaminyltransferase;
alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetylglucosaminyltransferase;
GnTV;
GlcNAc-T V;
UDP-N-acetyl-D-glucosamine:6-[2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl]-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)

Reaction(IUBMB)

UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] [RN:R05991]

Reaction(KEGG)

R05991(G);
(other) R04665

Substrate

UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]

Product

UDP [CPD:C00015];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]

Comment

Requires Mg2+. The enzyme, found in vertebrates, participates in the processing of N-glycans in the Golgi apparatus. It catalyses the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides, and thus enables the synthesis of tri- and tetra-antennary complexes.

History

EC 2.4.1.155 created 1986, modified 2001, modified 2018

Pathway

ec00510

N-Glycan biosynthesis

ec01100

Metabolic pathways

Orthology

K00744

alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetyl-glucosaminyltransferase

Genes

HSA:	4249(MGAT5)

PTR:	459625(MGAT5)

PPS:	100989629(MGAT5)

GGO:	101129003(MGAT5)

PON:	100445523(MGAT5)

NLE:	100597446(MGAT5)

HMH:	116462886(MGAT5)

MCC:	709108(MGAT5)

MCF:	102123398(MGAT5)

MTHB:

126932233

MNI:	105492547(MGAT5)

CSAB:

103216975(MGAT5)

CATY:

105590337(MGAT5)

PANU:

100999378(MGAT5)

TGE:	112636563(MGAT5)

MLEU:

105532207(MGAT5)

RRO:	104654400(MGAT5)

RBB:	108539646(MGAT5)

TFN:	117095089(MGAT5)

PTEH:

111527477(MGAT5)

CANG:

105508785(MGAT5)

CJC:	100414526(MGAT5)

SBQ:	101038866(MGAT5)

CIMI:

108309638(MGAT5)

CSYR:

103271940(MGAT5)

MMUR:

105857612(MGAT5)

LCAT:

123644015(MGAT5)

PCOQ:

105815371(MGAT5)

OGA:	100967000(MGAT5)

MMU:	107895(Mgat5)

MCAL:

110290015(Mgat5)

MPAH:

110321291(Mgat5)

RNO:	65271(Mgat5)

MCOC:

116098324(Mgat5)

ANU:	117716135(Mgat5)

MUN:	110551079(Mgat5)

CGE:	100760162(Mgat5)

MAUA:

101823312(Mgat5)

PROB:

127236329(Mgat5)

PLEU:

114696950(Mgat5)

MORG:

121445975(Mgat5)

MFOT:

126513228

AAMP:

119827993(Mgat5)

NGI:	103733594(Mgat5)

HGL:	101721991(Mgat5)

CPOC:

100712879(Mgat5)

CCAN:

109689552 109689553

DORD:

105996638(Mgat5)

DSP:	122110739(Mgat5)

PLOP:

125350744(Mgat5)

NCAR:

124979610

MMMA:

107155737(Mgat5)

OCU:

100346879

OPI:	101529461(MGAT5)

TUP:	102497975(MGAT5)

GVR:

103600145

CFA:	483895(MGAT5)

CLUD:

112648584(MGAT5)

VVP:	112932611(MGAT5)

VLG:	121481799(MGAT5)

NPO:	129503655(MGAT5)

AML:	100483104(MGAT5)

UMR:	103667662(MGAT5)

UAH:	113263431(MGAT5)

UAR:	123782810(MGAT5)

ELK:

111141394

LLV:

125095948

MPUF:

101682188(MGAT5)

MNP:	132013934(MGAT5)

MLK:	131826947(MGAT5)

NVS:	122902713(MGAT5)

ORO:	101379628(MGAT5)

EJU:	114207232(MGAT5)

ZCA:	113919750(MGAT5)

MLX:	118012512(MGAT5)

NSU:	110585642(MGAT5)

LWW:	102727905(MGAT5)

FCA:	101088682(MGAT5)

PYU:	121030477(MGAT5)

PCOO:

112865728(MGAT5)

PBG:	122479353(MGAT5)

PVIV:

125172161(MGAT5)

LRUF:

124515327

PTG:	102969054(MGAT5)

PPAD:

109270081(MGAT5)

PUC:

125911908

AJU:

106983003

HHV:	120230385(MGAT5)

BTA:	537595(MGAT5)

BOM:	102275628(MGAT5)

BIU:	109568385(MGAT5)

BBUB:

102393516(MGAT5)

BBIS:

104998474(MGAT5)

CHX:	102174983(MGAT5)

OAS:	101107672(MGAT5)

BTAX:

128070801(MGAT5)

ODA:	120854416(MGAT5)

CCAD:

122454020(MGAT5)

MBEZ:

129538435(MGAT5)

SSC:	100512563(MGAT5)

CFR:	102521418(MGAT5)

CBAI:

105083402(MGAT5)

CDK:	105092441(MGAT5)

VPC:	102538273(MGAT5)

BACU:

102997655(MGAT5)

BMUS:

118898461(MGAT5)

LVE:	103072240(MGAT5)

OOR:	101288542(MGAT5)

DLE:	111172696(MGAT5)

PSIU:

116757192(MGAT5)

NASI:

112412405(MGAT5)

ECB:	100050356(MGAT5)

EPZ:	103555913(MGAT5)

EAI:	106836533(MGAT5)

MYB:	102246850(MGAT5)

MYD:	102761898(MGAT5)

MMYO:

118661808(MGAT5)

MLF:	102427244(MGAT5)

PKL:	118709849(MGAT5)

EFUS:

103287756(MGAT5)

MNA:	107531999(MGAT5)

DRO:	112313014(MGAT5)

SHON:

119002628(MGAT5)

AJM:

119041278

PDIC:

114493783(MGAT5)

PHAS:

123810334(MGAT5)

MMF:	118625823(MGAT5)

PPAM:

129077551(MGAT5)

HAI:	109374684(MGAT5)

RFQ:	117026018(MGAT5)

PALE:

102884190(MGAT5)

PGIG:

120611924(MGAT5)

PVP:	105288371(MGAT5)

RAY:	107520333(MGAT5)

MJV:	108406913(MGAT5)

TOD:	119255776(MGAT5)

SARA:

101542429(MGAT5)

LAV:	100664057(MGAT5)

TMU:

101345363

ETF:	101644386(MGAT5)

DNM:	101421414(MGAT5)

MDO:	100011845(MGAT5)

GAS:	123242284(MGAT5)

SHR:	100929992(MGAT5)

AFZ:

127554350

PCW:	110223747(MGAT5)

OAA:	100091600(MGAT5)

GGA:	424286(MGAT5)

PCOC:

116227423(MGAT5)

MGP:	100541307(MGAT5)

CJO:	107316876(MGAT5)

TPAI:

128076750(MGAT5)

LMUT:

125697141(MGAT5)

NMEL:

110399356(MGAT5)

APLA:

101797784(MGAT5)

ACYG:

106039337(MGAT5)

CATA:

118252610(MGAT5)

AFUL:

116490710(MGAT5)

TGU:	100228988(MGAT5)

LSR:	110480631(MGAT5)

SCAN:

103814283(MGAT5)

PMOA:

120498970(MGAT5)

OTC:	121336517(MGAT5)

PRUF:

121356506(MGAT5)

GFR:	102042048(MGAT5)

FAB:	101814359(MGAT5)

OMA:	130255389(MGAT5)

PHI:	102101886(MGAT5)

PMAJ:

107207494(MGAT5)

CCAE:

111932311(MGAT5)

CCW:	104694065(MGAT5)

CBRC:

103619659(MGAT5)

ETL:	114062071(MGAT5)

ZAB:	102062481(MGAT5)

ACHL:

103804307(MGAT5)

SVG:	106849912(MGAT5)

MMEA:

130573807(MGAT5)

HRT:	120754948(MGAT5)

FPG:	101918414(MGAT5)

FCH:	102055854(MGAT5)

CLV:	102084918(MGAT5)

EGZ:	104129527(MGAT5)

NNI:	104009751(MGAT5)

PCRI:

104038971(MGAT5)

PLET:

104623623(MGAT5)

EHS:	104513702(MGAT5)

PCAO:

104049496(MGAT5)

ACUN:

113482013(MGAT5)

TALA:

104359020(MGAT5)

PADL:

103918490(MGAT5)

AFOR:

103899428(MGAT5)

ACHC:

115342453(MGAT5)

HALD:

104321335

HLE:	104836515(MGAT5)

AGEN:

126039664

GCL:

127018472

CCRI:

104167599(MGAT5)

CSTI:

104550915(MGAT5)

CMAC:

104481347(MGAT5)

MUI:

104542915

BREG:

104638929(MGAT5)

GSTE:

104264496(MGAT5)

LDI:	104350743(MGAT5)

MNB:	103774286(MGAT5)

OHA:	104333246(MGAT5)

NNT:	104407813(MGAT5)

SHAB:

115608408(MGAT5)

DPUB:

104296916(MGAT5)

PGUU:

104470122(MGAT5)

ACAR:

104527448(MGAT5)

CPEA:

104391149(MGAT5)

CVF:	104296035(MGAT5)

RTD:	128912457(MGAT5)

CUCA:

104065217(MGAT5)

TEO:	104377430(MGAT5)

BRHI:

104496526(MGAT5)

AAM:	106498453(MGAT5)

AROW:

112977057(MGAT5)

NPD:	112948582(MGAT5)

TGT:	104565378(MGAT5)

DNE:	112988311(MGAT5)

SCAM:

104145721(MGAT5)

ASN:	102386992(MGAT5)

AMJ:	102563315(MGAT5)

CPOO:

109322835(MGAT5)

GGN:	109301368(MGAT5)

PSS:	102455461(MGAT5)

CMY:	102946323(MGAT5)

CCAY:

125644768(MGAT5)

DCC:	119863325(MGAT5)

CPIC:

101938965(MGAT5)

TST:	117884678(MGAT5)

CABI:

116826090(MGAT5)

MRV:	120374683(MGAT5)

ACS:	100566008(mgat5)

ASAO:

132775100(MGAT5)

PVT:	110071860(MGAT5)

SUND:

121928031(MGAT5)

PBI:	103063095(MGAT5) 103064781

PMUR:

107284397(MGAT5)

CTIG:

120301251(MGAT5)

TSR:	106545857(MGAT5)

PGUT:

117669472(MGAT5)

APRI:

131203660(MGAT5)

PTEX:

113445467(MGAT5)

NSS:	113410714(MGAT5)

VKO:	123027373(MGAT5)

PMUA:

114604540(MGAT5)

PRAF:

128421944(MGAT5)

ZVI:	118092382(MGAT5)

HCG:	128326555(MGAT5)

GJA:	107108668(MGAT5) 107111387

STOW:

125425751(MGAT5)

EMC:	129324385(MGAT5)

XLA:	108701278 108702251

XTR:	100145551(mgat5)

NPR:	108792298(MGAT5)

RTEM:

120944163(MGAT5)

BBUF:

121008578(MGAT5)

BGAR:

122944471(MGAT5)

MUO:	115474045(MGAT5)

GSH:	117360653(MGAT5)

DRE:	724006(mgat5)

SRX:	107739338 107745986

SANH:

107653587 107663499

SGH:	107586987 107593035(mgat5)

CCAR:

109047295 109083375 109087669

CAUA:

113040766 113064774 113077639

CGIB:

127942982 127987986

PTET:

122327204(mgat5)

LROH:

127153327(mgat5)

OMC:	131530321(mgat5)

PPRM:

120486519(mgat5)

MAMB:

125253866(mgat5)

CIDE:

127520398

MASI:

127437982 127438771

TROS:

130548512(mgat5)

TDW:	130429627(mgat5)

MANU:

129454019(mgat5)

IPU:

108259240

IFU:	128603149(mgat5)

PHYP:

113547435(mgat5)

SMEO:

124379668(mgat5)

TFD:	113657033(mgat5)

TVC:	132838580(mgat5)

AMEX:

103037113(mgat5)

CMAO:

118800920(mgat5)

EEE:	113581466(mgat5)

CHAR:

105905647(mgat5)

TRU:	101072031(mgat5) 101074995

TFS:	130522668(mgat5) 130529365

TNG:	GSTEN00006450G001 GSTEN00013473G001

LCO:	104925340(mgat5)

NCC:	104941679(mgat5) 104962910

TBEN:

117485591 117498890(mgat5)

CGOB:

115020149(mgat5) 115026505

PGEO:

117456276(mgat5) 117466350

GACU:

117532443 117541541

EMAC:

134860808(mgat5) 134867712

ELY:	117249803(mgat5) 117251389

EFO:	125884675(mgat5) 125899720

PLEP:

121949308 121962674(mgat5)

SLUC:

116037737 116044574(mgat5)

ECRA:

117939637(mgat5)

ESP:	116673956(mgat5)

PFLV:

114551135 114563683

GAT:	120820174(mgat5) 120833820

PPUG:

119199315(mgat5) 119228632

AFB:	129104864 129111872(mgat5)

CLUM:

117746034(mgat5)

PSWI:

130204300(mgat5)

MSAM:

119902006(mgat5) 119903256

SCHU:

122872215(mgat5)

CUD:	121506333(mgat5)

ALAT:

119015474(mgat5) 119026392

MZE:	101481161(mgat5)

ONL:	100691248(mgat5)

OAU:	116334403(mgat5)

OLA:	101168186(mgat5)

OML:	112159997(mgat5)

CSAI:

133425058(mgat5) 133446507

XMA:	102232481(mgat5) 102236488

XCO:	114140432(mgat5) 114148135

XHE:	116715581(mgat5) 116723023

PRET:

103460330 103476254(mgat5)

PFOR:

103141415(mgat5) 103152097

PLAI:

106936559 106948945(mgat5)

PMEI:

106903323 106914659

GAF:	122827248(mgat5) 122839913

PPRL:

129374487 129379039(mgat5)

CVG:	107094135 107094137(mgat5)

CTUL:

119771852 119789711(mgat5)

GMU:	124861575(mgat5)

NFU:	107381041 107390173(mgat5)

KMR:	108238485 108246486(mgat5)

ALIM:

106519126 106519129 106529442

NWH:	119419033 119428955(mgat5)

AOCE:

111562783 111582951

MCEP:

125000738(mgat5) 125017276

CSEM:

103389683(mgat5)

POV:	109631205 109639749(mgat5)

SSEN:

122761506 122785633

HHIP:

117754947 117775326(mgat5)

HSP:	118103351(mgat5) 118120478

SMAU:

118283105 118309346(mgat5)

LCF:	108887100 108891873(mgat5)

SDU:	111218827 111222699(mgat5)

SLAL:

111646016(mgat5) 111667400

XGL:	120798018(mgat5) 120801806

HCQ:	109514989(mgat5)

SSCV:

125988646

SBIA:

133491418(mgat5)

PEE:	133410186(mgat5)

PTAO:

133488920(mgat5)

BPEC:

110153275(mgat5)

MALB:

109955689(mgat5) 109968552

BSPL:

114847546 114870384(mgat5)

SJO:	128354400(mgat5)

SASA:

106574313 106575089

STRU:

115179283 115195944

OTW:	112253925 112253994

OMY:	110495947 110527561

OGO:	124008692 124010544

ONE:	115129626 115134299

OKI:	109890693 109906649

OKE:	118363989 118370141

SALP:

111953416 111971109

SNH:	120033306 120051232

CCLU:

121554440

ELS:	105019667(mgat5)

SFM:	108929747 108941965(mgat5)

PKI:	111849087(mgat5) 111849468

AANG:

118213644(mgat5) 118222127

LOC:	102687035(mgat5)

PSPA:

121322345 121322621

ARUT:

117407598(mgat5) 117426936

PSEX:

120531680(mgat5)

LCM:	102363158(MGAT5)

CMK:	103180379(mgat5) 103184928

RTP:

109917938

CPLA:

122550236 122551703(mgat5)

HOC:	132815671 132817057(mgat5)

LERI:

129699028(mgat5) 129712590

PMRN:

116937422(MGAT5)

LRJ:	133346208(MGAT5)

BFO:

118405638

BBEL:

109478949

CIN:	100175898 108949978

SCLV:

120340156 120342409 120342410 120347147

SPU:

100888694

LPIC:

129254219

APLC:

110989436

AJC:

117109570

SKO:

100366560

CEL:	CELE_C55B7.2(gly-2)

CBR:	CBG_12645(Cbr-gly-2)

CRQ:	GCK72_002835

NAI:	NECAME_14592

TSP:

Tsp_11593

HRO:	HELRODRAFT_115589 HELRODRAFT_180890

LGI:	LOTGIDRAFT_172419

PVUL:

126831976

PCAN:

112565538

BGT:

106078612

GAE:

121387511

HRF:

124149529

HRJ:

124253233

CRG:

105330629

CVN:

111124498

CANU:

128191865

OED:

125678948

MYI:

110458649

PMAX:

117317713

MCAF:

127717647

MMER:

123538281

RPHI:

132752966

DPOL:

127846997

OBI:

106880226

OSN:

115223971

LAK:	106160238 106160396 106171734 106172929 106177233 106181457

SMM:	Smp_040510 Smp_144260

SHX:	MS3_00002227(MGAT5_1)

OVI:	T265_04472

NVE:	5509104 5517864 5517897 5517929 5521677

EPA:	110232340 110232355 110232390 110241019 110241101 110244956

ATEN:

116288093 116288103 116295791 116306769 116308527 116308528

ADF:	107327239 107337307 107345019 107345720 107345743 107347453 107353933

AMIL:

114954488 114954490 114957136 114961709

PDAM:

113671483 113678068 113683774 113683775 113683780

SPIS:

111327556 111327561 111332554 111336901

DGT:	114517678 114520371 114522055 114527192

XEN:	124441138 124450082 124453287

HMG:	100200937 100214696

HSY:	130614785 130614786 130629732 130641709

» show all

Reference

1 [PMID:6216250]

Authors

Cummings RD, Trowbridge IS, Kornfeld S.

Title

A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.

Journal

J Biol Chem 257:13421-7 (1982)

Reference

2 [PMID:2834054]

Authors

Hindsgaul O, Tahir SH, Srivastava OP, Pierce M.

Title

The trisaccharide beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp, as its 8-methoxycarbonyloctyl glycoside, is an acceptor selective for N-acetylglucosaminyltransferase V.

Journal

Carbohydr Res 173:263-72 (1988)
DOI:10.1016/S0008-6215(00)90822-X

Reference

3 [PMID:1531335]

Authors

Shoreibah MG, Hindsgaul O, Pierce M

Title

Purification and characterization of rat kidney UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,6-N-acetylglucosaminyltransferase.

Journal

J Biol Chem 267:2920-7 (1992)

Reference

4 [PMID:8393437]

Authors

Gu J, Nishikawa A, Tsuruoka N, Ohno M, Yamaguchi N, Kangawa K, Taniguchi N

Title

Purification and characterization of UDP-N-acetylglucosamine: alpha-6-D-mannoside beta 1-6N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase V) from a human lung cancer cell line.

Journal

J Biochem 113:614-9 (1993)
DOI:10.1093/oxfordjournals.jbchem.a124091

Reference

5 [PMID:10395745]

Authors

Park C, Jin UH, Lee YC, Cho TJ, Kim CH

Title

Characterization of UDP-N-acetylglucosamine:alpha-6-d-mannoside beta-1,6-N-acetylglucosaminyltransferase V from a human hepatoma cell line Hep3B.

Journal

Arch Biochem Biophys 367:281-8 (1999)
DOI:10.1006/abbi.1999.1252

Sequence

[hsa:4249]

Reference

6 [PMID:11872751]

Authors

Saito T, Miyoshi E, Sasai K, Nakano N, Eguchi H, Honke K, Taniguchi N

Title

A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V distinct from the original glycosyltransferase activity.

Journal

J Biol Chem 277:17002-8 (2002)
DOI:10.1074/jbc.M200521200

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.155

IUBMB Enzyme Nomenclature:

2.4.1.155

ExPASy - ENZYME nomenclature database:

2.4.1.155

BRENDA, the Enzyme Database:

2.4.1.155

CAS:	83588-90-3

DBGET integrated database retrieval system