Entry
Name
dolichyl-P-Man:Man7GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase;
ALG12;
ALG12 mannosyltransferase;
ALG12 alpha1,6mannosyltransferase;
dolichyl-P-mannose:Man7GlcNAc2-PP-dolichyl mannosyltransferase;
dolichyl-P-Man:Man7GlcNAc2-PP-dolichyl alpha6-mannosyltransferase;
EBS4;
Dol-P-Man:Man7GlcNAc2-PP-Dol alpha-1,6-mannosyltransferase;
dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,6-mannosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dolichyl beta-D-mannosyl-phosphate:alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-beta-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol 6-alpha-D-mannosyltransferase (configuration-inverting)
Reaction(IUBMB)
dolichyl beta-D-mannosyl phosphate + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-beta-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = alpha-D-Man-alpha-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:
R06260 ]
Reaction(KEGG)
Substrate
dolichyl beta-D-mannosyl phosphate [CPD:
C03862 ];
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-beta-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Product
alpha-D-Man-alpha-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:
C00110 ]
Comment
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9Glc-NAc2-PP-Dol on the lumenal side use dolichyl beta-D-mannosyl phosphate.
History
EC 2.4.1.260 created 1976 as EC 2.4.1.130, part transferred 2011 to EC 2.4.1.160, modified 2012
Pathway
ec00513 Various types of N-glycan biosynthesis
Orthology
K03847 alpha-1,6-mannosyltransferase
Genes
VLG : 121491169(ALG12) 121497486
MLX : 117997755(ALG12) 118012103
ZVI : 118091452(ALG12) 118091486
CCAR : 109055011 109088012
PTET : 122335470 122342622(alg12)
SASA : 100195409(alg12) 106609155
CCLU : 121555199 121581251
ARUT : 117415153 117419504 117968879
DSI : Dsimw501_GD18643(Dsim_GD18643)
AALB : 109428475 115260009
DPX : DAPPUDRAFT_307223 DAPPUDRAFT_340335
PTEP : 107438060 122268870
CEL : CELE_ZC513.5(algn-12)
CSAT : 104758180 104773533 104773734
BNA : 106370825 106400825 106426032 106434177
RSZ : 108806148 108857290 130507983
LJA : Lj0g3v0165139.2(Lj0g3v0165139.2) Lj3g3v0594260.1(Lj3g3v0594260.1) Lj3g3v0594260.2(Lj3g3v0594260.2)
PCIN : 129289931 129291074
SSPL : 121775114 121802383 121808053
PSOM : 113284616 113343855
DOSA : Os04t0544900-01(Os04g0544900)
TAES : 123046208 123054085 123189971
PVIR : 120642645 120682558
SMO : SELMODRAFT_91475(GT22A3)
MPP : MICPUCDRAFT_22710(JGI:MicpuC2_22710)
NCS : NCAS_0A05360(NCAS0A05360)
NDI : NDAI_0K02210(NDAI0K02210)
TPF : TPHA_0M01820(TPHA0M01820)
TBL : TBLA_0F04110(TBLA0F04110)
TDL : TDEL_0A07530(TDEL0A07530)
KAF : KAFR_0C04580(KAFR0C04580)
KNG : KNAG_0D00400(KNAG0D00400)
CAL : CAALFM_C702970WA(ECM39)
NTE : NEUTE1DRAFT88888(NEUTE1DRAFT_88888)
PBEL : QC761_106880(ECM39)
PPSD : QC762_106880(ECM39)
PPSP : QC763_106880(ECM39)
PPSA : QC764_106880(ECM39)
PTKZ : JDV02_001673(ECM39)
ALUC : AKAW2_20899A(ALG12)
CNG : CNAG_02715 CNAG_07527
CCAC : CcaHIS019_0404510(ECM39)
PCO : PHACADRAFT_180449 PHACADRAFT_214615
SHS : STEHIDRAFT_165833 STEHIDRAFT_73504
RSX : RhiXN_02631 RhiXN_02632
LBC : LACBIDRAFT_302539(ALG12)
ABP : AGABI1DRAFT75475(AGABI1DRAFT_75475)
ABV : AGABI2DRAFT184199(AGABI2DRAFT_184199)
SCM : SCHCO_02487496(SCHCODRAFT_02487496)
SPAR : SPRG_11694 SPRG_16521
» show all
Taxonomy
Reference
Authors
Frank CG, Aebi M.
Title
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
Journal
Sequence
Reference
Authors
Hong Z, Jin H, Fitchette AC, Xia Y, Monk AM, Faye L, Li J
Title
Mutations of an alpha1,6 mannosyltransferase inhibit endoplasmic reticulum-associated degradation of defective brassinosteroid receptors in Arabidopsis.
Journal
Sequence
Reference
Authors
Cipollo JF, Trimble RB
Title
The Saccharomyces cerevisiae alg12delta mutant reveals a role for the middle-arm alpha1,2Man- and upper-arm alpha1,2Manalpha1,6Man- residues of Glc3Man9GlcNAc2-PP-Dol in regulating glycoprotein glycan processing in the endoplasmic reticulum and Golgi apparatus.
Journal
Reference
Authors
Grubenmann CE, Frank CG, Kjaergaard S, Berger EG, Aebi M, Hennet T
Title
ALG12 mannosyltransferase defect in congenital disorder of glycosylation type lg.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.4.1.260