KEGG   ENZYME: 2.4.1.264
Entry
EC 2.4.1.264                Enzyme                                 
Name
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronosyltransferase;
GumK;
UDP-glucuronate:D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol beta-1,2-glucuronyltransferase;
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-alpha-D-glucuronate:alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol beta-1,2-glucuronosyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-alpha-D-glucuronate + alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol = UDP + beta-D-GlcA-(1->2)-alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol [RN:R09732]
Reaction(KEGG)
R09732
Substrate
UDP-alpha-D-glucuronate [CPD:C00167];
alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol
Product
UDP [CPD:C00015];
beta-D-GlcA-(1->2)-alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol
Comment
The enzyme is involved in the biosynthesis of the exopolysaccharides xanthan (in the bacterium Xanthomonas campestris) and acetan (in the bacterium Gluconacetobacter xylinus).
History
EC 2.4.1.264 created 2011, modified 2016
Orthology
K13659  2-beta-glucuronyltransferase
Genes
XFA: XF_2361
XFT: PD_1388(gumK)
XFM: Xfasm12_1530
XFN: XfasM23_1474
XFF: XFLM_01075
XFL: P303_06655
XFS: D934_11035
XFH: XFHB_03295
XTW: AB672_04685
XCC: XCC2445(gumK)
XCB: XC_1667
XCA: xcc-b100_1721(gumK)
XCP: XCR_2758(gumK)
XCV: XCV2778(gumK)
XAX: XACM_2558(gumK)
XAC: XAC2576(gumK)
XCI: XCAW_02257(gumK)
XCT: J151_02757
XCJ: J158_02744
XCU: J159_02742
XCN: J169_02754
XCW: J162_02746
XCR: J163_02741
XCM: J164_02743
XCF: J172_02748
XFU: XFF4834R_chr26130(gumK)
XAO: XAC29_13120
XOM: XOO3010(XOO3010)
XOO: XOO3170(gumK)
XOP: PXO_01401(gumK)
XOY: AZ54_08015
XOR: XOC_1876(gumK)
XOZ: BE73_08805
XTN: FD63_08030
XFR: BER92_06085
XVE: BJD12_03910
XPE: BJD13_03595
XHR: XJ27_06760
XGA: BI317_09575
XPH: XppCFBP6546_19515(XppCFBP6546P_19515)
XVA: C7V42_09290
XAN: AC801_09290
XAR: XB05_07770
XHY: FZ025_16445
XCZ: EBN15_09455
XEU: XSP_002559
XHD: LMG31886_17120(gumK)
XPR: MUG10_18770
XAG: HEP73_01778
XDY: NYR95_14140
XAS: HEP74_01696
PSD: DSC_06260 DSC_13670
PWI: MWN52_13240 MWN52_14780
CJA: CJA_0618(gt70A)
CEB: B0D95_08985
CELL: CBR65_10400
CEK: D0B88_17285
CEG: D0C16_09515
GLC: JQN73_13840 JQN73_17615
SPLB: SFPGR_10350(gumK)
ABRE: pbN1_39130
DEY: HYN24_07370
AZO: azo2235(gumK)
AOA: dqs_2368
AZR: CJ010_14190
SIX: BSY16_5351
AVV: RvVAT039_12250
AVF: RvVAR031_32160
AVI: Avi_6016
RLS: HB780_18345
ARA: Arad_3074
PDES: FE840_000810 FE840_001985
MEDK: QEV83_18795
TSO: IZ6_26580
SULI: C1J05_21115
ROT: FIV09_19275(gumK)
SAER: NEF64_03640
SABI: PBT88_06970
SJP: SJA_C2-01910(gumK)
SYB: TZ53_09480
SYA: A6768_08600
SUFL: FIL70_23600
SBAR: H5V43_20185
SPPH: KFK14_12210
GDI: GDI2542(gumK)
GDJ: Gdia_0782
GXY: GLX_27750(gumK)
GXL: H845_816
KNA: B0W47_14415
KEU: S101446_03142(gumK)
KRE: GWK63_04890
KOI: LV478_10000
AACE: A0U92_11530
KBA: A0U89_05135
NGU: QN315_03685
RCE: RC1_2535(gumK)
NOS: Nos7107_0795
CALH: IJ00_16875
FBT: D770_17675
MRTJ: KHC33_15105
 » show all
Reference
1  [PMID:9537354]
  Authors
Katzen F, Ferreiro DU, Oddo CG, Ielmini MV, Becker A, Puhler A, Ielpi L
  Title
Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence.
  Journal
J Bacteriol 180:1607-17 (1998)
DOI:10.1128/JB.180.7.1607-1617.1998
Reference
2  [PMID:7683019]
  Authors
Ielpi L, Couso RO, Dankert MA
  Title
Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris.
  Journal
J Bacteriol 175:2490-500 (1993)
DOI:10.1128/JB.175.9.2490-2500.1993
Reference
3  [PMID:18854951]
  Authors
Kim SY, Kim JG, Lee BM, Cho JY
  Title
Mutational analysis of the gum gene cluster required for xanthan biosynthesis in  Xanthomonas oryzae pv oryzae.
  Journal
Biotechnol Lett 31:265-70 (2009)
DOI:10.1007/s10529-008-9858-3
Reference
4  [PMID:16946469]
  Authors
Barreras M, Bianchet MA, Ielpi L
  Title
Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 62:880-3 (2006)
DOI:10.1107/S1744309106028764
  Sequence
[ag:AAN86640]
Reference
5  [PMID:18596046]
  Authors
Barreras M, Salinas SR, Abdian PL, Kampel MA, Ielpi L
  Title
Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase.
  Journal
J Biol Chem 283:25027-35 (2008)
DOI:10.1074/jbc.M801227200
  Sequence
[ag:AAN86640]
Reference
6  [PMID:11841812]
  Authors
Vojnov AA, Bassi DE, Daniels MJ, Dankert MA
  Title
Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris.
  Journal
Carbohydr Res 337:315-26 (2002)
DOI:10.1016/S0008-6215(01)00322-6
Reference
7  [PMID:14736729]
  Authors
Barreras M, Abdian PL, Ielpi L
  Title
Functional characterization of GumK, a membrane-associated beta-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis.
  Journal
Glycobiology 14:233-41 (2004)
DOI:10.1093/glycob/cwh056
  Sequence
[ag:AAN86640]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.264
IUBMB Enzyme Nomenclature: 2.4.1.264
ExPASy - ENZYME nomenclature database: 2.4.1.264
BRENDA, the Enzyme Database: 2.4.1.264

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Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Gene (112)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (105)   
   KEGG MGENES (3)   
   RefGene (3)   
Protein sequence (45)   
   UniProt (43)   
   SWISS-PROT (2)   
DNA sequence (22)   
   GenBank (11)   
   EMBL (11)   
All databases (186)   

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