KEGG ENZYME: 2.4.1.47

ENZYME: 2.4.1.47

Entry

EC 2.4.1.47                 Enzyme

Name

N-acylsphingosine galactosyltransferase;
UGT8 (gene name);
CGT (gene name);
UDP galactose-N-acylsphingosine galactosyltransferase;
uridine diphosphogalactose-acylsphingosine galactosyltransferase;
UDP-galactose:N-acylsphingosine D-galactosyltransferase;
UDP-alpha-D-galactose:N-acylsphingosine D-galactosyltransferase;
2-hydroxyacylsphingosine 1-beta-galactosyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-alpha-D-galactose:N-acylsphingosine beta-D-galactosyltransferase (configuration-inverting)

Reaction(IUBMB)

UDP-alpha-D-galactose + a ceramide = UDP + a beta-D-galactosylceramide [RN:R01500 R06277]

Reaction(KEGG)

R01500 R06277(G) > R04322;
(other) R01928 R10804

Substrate

UDP-alpha-D-galactose [CPD:C00052];
ceramide [CPD:C00195]

Product

UDP [CPD:C00015];
beta-D-galactosylceramide

Comment

This membrane-bound, endoplasmic reticulum-located enzyme catalyses the last step in the synthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. It has a strong preference for ceramides that contain hydroxylated fatty acids.

History

EC 2.4.1.47 created 1972

Pathway

ec00565

Ether lipid metabolism

ec00600

Sphingolipid metabolism

ec01100

Metabolic pathways

Orthology

K04628

ceramide galactosyltransferase

Genes

HSA:	7368(UGT8)

PTR:	461446(UGT8)

PPS:	100985864(UGT8)

GGO:	101137553(UGT8)

PON:	100437492(UGT8)

NLE:	100607960(UGT8)

HMH:	116463268(UGT8)

MCC:	702979(UGT8)

MCF:	102126400(UGT8)

MTHB:

126954282

MNI:	105486216(UGT8)

CSAB:

103236153(UGT8)

CATY:

105571468(UGT8)

PANU:

101011998(UGT8)

TGE:	112624639(UGT8)

MLEU:

105553404(UGT8)

RRO:	104675972(UGT8)

RBB:	108527094(UGT8)

TFN:	117085818(UGT8)

PTEH:

111547934(UGT8)

CANG:

105516262(UGT8)

CJC:	100396627(UGT8)

SBQ:	101036737(UGT8)

CIMI:

108290692(UGT8)

CSYR:

103261614(UGT8)

MMUR:

105879492(UGT8)

LCAT:

123627686(UGT8)

PCOQ:

105818926(UGT8)

OGA:	100958105(UGT8)

MMU:	22239(Ugt8a)

MCAL:

110291396(Ugt8)

MPAH:

110320435(Ugt8)

RNO:	50555(Ugt8)

MCOC:

116093746(Ugt8)

ANU:	117707751(Ugt8)

MUN:	110539856(Ugt8)

CGE:

100772408

MAUA:

101840830(Ugt8)

PROB:

127223058(Ugt8)

PLEU:

114684609(Ugt8)

MORG:

121453672(Ugt8)

MFOT:

126508627

AAMP:

119801211(Ugt8)

NGI:	103731259(Ugt8)

HGL:	101702896(Ugt8)

CPOC:

100718939(Ugt8)

CCAN:

109677276(Ugt8)

DORD:

105991238(Ugt8)

DSP:	122123194(Ugt8)

PLOP:

125341473(Ugt8)

NCAR:

124994017

MMMA:

107141407(Ugt8)

OCU:

100341450

OPI:	101523880(UGT8)

TUP:	102480796(UGT8)

GVR:	103581098(UGT8)

CFA:	487910(UGT8)

CLUD:

112671694(UGT8)

VVP:	112930967(UGT8)

VLG:	121493881(UGT8)

NPO:	129521117(UGT8)

AML:	100465291(UGT8)

UMR:	103661766(UGT8)

UAH:	113255592(UGT8)

UAR:	123793338(UGT8)

ELK:

111156987

LLV:

125093308

MPUF:

101693531(UGT8)

MNP:	132002626(UGT8)

MLK:	131828786(UGT8)

NVS:	122889228(UGT8)

ORO:	101383800(UGT8)

EJU:	114203048 114203049 114222677(UGT8)

ZCA:	113924512(UGT8)

MLX:	118027111(UGT8)

NSU:	110575490(UGT8)

LWW:	102743653(UGT8)

FCA:	101097722(UGT8)

PYU:	121030616(UGT8)

PCOO:

112858491(UGT8)

PBG:	122476845(UGT8)

PVIV:

125164670(UGT8)

LRUF:

124519935

PTG:	102965984(UGT8)

PPAD:

109248503(UGT8)

PUC:

125923047

AJU:

106977315

HHV:	120225123(UGT8)

BTA:	281566(UGT8)

BOM:	102269259(UGT8)

BIU:	109560234(UGT8)

BBUB:

102409846(UGT8)

BBIS:

105002052(UGT8)

CHX:	102174638(UGT8)

OAS:	101113069(UGT8)

BTAX:

128049872(UGT8)

ODA:	120861700(UGT8)

CCAD:

122422350(UGT8)

MBEZ:

129561784(UGT8)

SSC:	100511825(UGT8)

CFR:	102522568(UGT8)

CBAI:

105067140(UGT8)

CDK:	105089918(UGT8)

VPC:	102525011(UGT8)

BACU:

103004850(UGT8)

BMUS:

118895154(UGT8)

LVE:	103073438(UGT8)

OOR:	101289765(UGT8)

DLE:	111186063(UGT8)

PCAD:

102993924(UGT8)

PSIU:

116754771(UGT8)

NASI:

112403739(UGT8)

ECB:	100072587(UGT8)

EPZ:	103557103(UGT8)

EAI:	106842868(UGT8)

MYB:	102252842(UGT8)

MMYO:

118652966(UGT8)

MLF:	102442855(UGT8)

PKL:	118726050(UGT8)

EFUS:

103298157(UGT8)

MNA:	107527850(UGT8)

DRO:	112312608(UGT8)

SHON:

118980012(UGT8)

AJM:	119037686(UGT8)

PDIC:

114503128(UGT8)

PHAS:

123814153(UGT8)

MMF:	118616083(UGT8)

PPAM:

129061384(UGT8)

HAI:	109390271(UGT8)

RFQ:	117038197(UGT8)

PALE:

102881397(UGT8)

PGIG:

120589341(UGT8)

PVP:

105309073

RAY:	107510863(UGT8)

MJV:	108409879(UGT8)

TOD:	119242760(UGT8)

SARA:

101551134(UGT8)

SETR:

126027875(UGT8)

LAV:	100665032(UGT8)

TMU:

101352658

ETF:	101655030(UGT8)

DNM:	101410626(UGT8)

MDO:	100010696(UGT8)

GAS:	123253133(UGT8)

SHR:	100927545(UGT8)

AFZ:

127540296

PCW:	110218601(UGT8)

OAA:	100076336(UGT8)

GGA:	374033(UGT8) 428949(UGT8L)

PCOC:

116231252(UGT8) 116234480

MGP:	100539821 100541199(UGT8)

CJO:	107313583(UGT8) 107315928

TPAI:

128077990 128080106(UGT8)

LMUT:

125691974(UGT8) 125693947

NMEL:

110399157(UGT8) 110400413

APLA:

101794817 101799126(UGT8)

ACYG:

106029878 106038627(UGT8)

CATA:

118243154 118244232(UGT8)

AFUL:

116489127(UGT8) 116491423

TGU:	100228968 100231902(UGT8)

LSR:	110471176(UGT8) 110480032

SCAN:

103813974 103815551(UGT8)

PMOA:

120502064 120502813(UGT8)

OTC:	121331975(UGT8) 121341851

PRUF:

121349178(UGT8) 121350254

GFR:	102031520(UGT8) 102040819

FAB:	101807254 101809664(UGT8)

OMA:	130252607(UGT8) 130254856

PHI:	102107687(UGT8) 102108262

PMAJ:

107203102(UGT8) 107206597

CCAE:

111928999(UGT8) 111931100

CCW:	104696845(UGT8) 104697059

CBRC:

103613199(UGT8) 103624465

ETL:	114054721 114068337(UGT8)

ZAB:	102062618(UGT8) 102066639

ACHL:

103803439(UGT8) 103804160

SVG:	106855308 106860196(UGT8)

MMEA:

130578784 130581403(UGT8)

HRT:	120752805(UGT8) 120755845

FPG:	101914948(UGT8) 101924669

FCH:	102047748 102051681(UGT8)

CLV:	102089904(UGT8) 102097199

EGZ:	104127441 104129123 104135946

NNI:	104019136(UGT8) 104019447

PCRI:

104036395

PLET:

104615011(UGT8) 104622396

EHS:	104504187(UGT8) 104514948

PCAO:

104047043(UGT8) 104051037

ACUN:

113479767(UGT8) 113481588

TALA:

104362911 104366712(UGT8)

PADL:

103915563 103926098(UGT8)

AFOR:

103897613 103901699(UGT8)

ACHC:

115344526(UGT8) 115348607

HALD:

104315039

HLE:	104831885 104836800(UGT8)

AGEN:

126042735 126044880

GCL:	127015661 127017771

CCRI:

104160879 104161821(UGT8)

CSTI:

104559430

CMAC:

104476709 104487754

MUI:	104536489(UGT8) 104543684

BREG:

104630313 104635471 104637738

FGA:	104070646 104075856(UGT8)

GSTE:

104259532(UGT8) 104264940

LDI:

104351754

MNB:	103768724(UGT8) 103769785

OHA:	104334458 104336717(UGT8)

NNT:

104406016

SHAB:

115605542(UGT8) 115608919

DPUB:

104297688 104310337(UGT8)

PGUU:

104459329(UGT8) 104466261

ACAR:

104518558 104521111

CPEA:

104391481 104393373(UGT8)

AVIT:

104273583 104276343 104281458

CVF:	104289747 104295651(UGT8)

RTD:	128910444(UGT8) 128911321

CUCA:

104059087(UGT8) 104067908

TEO:	104370543 104376593(UGT8)

BRHI:

104492239 104499174(UGT8)

AAM:	106492128(UGT8) 106498896

AROW:

112967517 112974891 112977284

NPD:	112943430(UGT8) 112957115

TGT:	104569610(UGT8) 104573043

DNE:	112980201 112986157(UGT8)

SCAM:

104138032(UGT8) 104141791

ASN:	102382334(UGT8) 102384980

AMJ:	102574992 106737104(UGT8)

CPOO:

109309361(UGT8) 109324295

GGN:	109287412 109301099(UGT8)

PSS:	102457655 102462979

CMY:	102936934(UGT8) 102941238

CCAY:

125635522(UGT8) 125640339

DCC:	119855314(UGT8) 119859521

CPIC:

101945157 101952125(UGT8)

TST:	117877789(UGT8) 117881007

CABI:

116823594 116825360(UGT8)

MRV:	120406330(UGT8) 120408926

ACS:	100551810(ugt8)

ASAO:

132776900(UGT8)

PVT:	110083901(UGT8)

SUND:

121930338(UGT8)

PBI:	103055483(UGT8)

PMUR:

107296372(UGT8)

CTIG:

120305635(UGT8)

TSR:	106556413(UGT8)

PGUT:

117679964(UGT8)

APRI:

131203332(UGT8)

PTEX:

113434591(UGT8)

NSS:	113412800(UGT8)

VKO:	123022823(UGT8)

PMUA:

114597016 114604195 114604507

PRAF:

128413684 128421200(UGT8)

ZVI:	118083636(UGT8) 118096504

HCG:	128327900(UGT8) 128349455

GJA:	107110289(UGT8) 107122753

STOW:

125440230(UGT8)

EMC:	129332432 129336897(UGT8)

XLA:	108706712(ugt8.S) 108710441(ugt8.L)

XTR:	779534(ugt8)

NPR:	108792209(UGT8)

RTEM:

120918452(UGT8)

BBUF:

120989896(UGT8)

BGAR:

122946095 122946158

MUO:	115462257 115462725(UGT8)

GSH:	117357337(UGT8)

DRE:	553508(ugt8)

SRX:	107745064 107750420

SANH:

107656191 107702381

SGH:	107548345(ugt8) 107591551

CCAR:

109045590 109058520(ugt8) 109105396

CAUA:

113044224 113106881

CGIB:

127949701 127960912

PTET:

122346325(ugt8)

LROH:

127168207(ugt8)

OMC:	131549221(ugt8)

PPRM:

120493749(ugt8)

RKG:	130089532(ugt8)

MAMB:

125272607(ugt8)

CIDE:

127518492

MASI:

127444375 127444494

TROS:

130553290(ugt8)

TDW:	130409582(ugt8)

MANU:

129444715(ugt8)

IPU:

108260842

IFU:	128604225(ugt8)

PHYP:

113544363(ugt8)

SMEO:

124381495(ugt8)

TFD:	113660716(ugt8)

TVC:	132841903(ugt8)

AMEX:

103043793(ugt8)

CMAO:

118816521(ugt8)

EEE:	113586472(ugt8)

CHAR:

105903570(ugt8)

TRU:	101064956(ugt8)

TFS:	130527446(ugt8)

TNG:	GSTEN00007473G001

LCO:	104929353(ugt8)

NCC:	104955311(ugt8)

TBEN:

117474752(ugt8)

CGOB:

115012015(ugt8)

PGEO:

117444397(ugt8)

GACU:

117543403(ugt8)

EMAC:

134864705(ugt8)

ELY:	117255548(ugt8)

EFO:	125886638(ugt8)

PLEP:

121942478(ugt8)

SLUC:

116052239(ugt8)

ECRA:

117959889(ugt8)

ESP:	116699279(ugt8)

PFLV:

114546530(ugt8)

GAT:	120825483(ugt8)

PPUG:

119218134(ugt8)

AFB:	129096381(ugt8)

CLUM:

117739477(ugt8)

PSWI:

130190681(ugt8)

MSAM:

119899023(ugt8)

SCHU:

122873821(ugt8)

CUD:	121509021(ugt8)

ALAT:

119021954(ugt8)

MZE:	101473219(ugt8)

ONL:	100696128(ugt8)

OAU:	116329078(ugt8)

OLA:	101156303(ugt8)

OML:	112157358(ugt8)

CSAI:

133462816(ugt8)

XMA:	102221616(ugt8)

XCO:	114144945(ugt8)

XHE:	116720325(ugt8)

PRET:

103467322(ugt8)

PFOR:

103137882(ugt8) 103137940

PLAI:

106943362 106947345(ugt8)

PMEI:

106923498 106928604(ugt8)

GAF:	122829438(ugt8)

CVG:	107085760(ugt8)

CTUL:

119772592(ugt8)

GMU:	124868442(ugt8)

NFU:	107375211(ugt8)

KMR:	108231012(ugt8)

ALIM:

106517139(ugt8)

NWH:	119408100(ugt8)

AOCE:

111568569(ugt8)

MCEP:

125004193(ugt8)

CSEM:

103383812(ugt8)

POV:	109637829(ugt8)

SSEN:

122771324(ugt8)

HHIP:

117763218(ugt8)

HSP:	118120929(ugt8)

SMAU:

118312703(ugt8)

LCF:

108896622

SDU:	111231488(ugt8)

SLAL:

111664066(ugt8)

XGL:	120799673(ugt8)

HCQ:	109519902(ugt8)

SSCV:

125968884

SBIA:

133505744(ugt8)

PEE:	133404436(ugt8)

PTAO:

133477468(ugt8)

BPEC:

110165331(ugt8)

MALB:

109961392(ugt8)

BSPL:

114859050(ugt8)

SJO:	128370901(ugt8)

STRU:

115184006 115184007

OTW:

121844288

OMY:	110512130 110516863

OGO:

124021864

OKI:	116360057 116372071

OKE:	127926863 127927878(ugt8)

CCLU:

121562104(ugt8) 121563379

ELS:	105009093(ugt8)

SFM:	108924325(ugt8)

PKI:	111837341(ugt8)

AANG:

118227920(ugt8)

LOC:	102684913(ugt8)

PSPA:

121295410 121322782

ARUT:

117409393

PSEX:

120533017(ugt8) 120538937

LCM:	102350700 102364207(UGT8)

CMK:	103181594 103188741(ugt8) 121851509

RTP:	109916958 125483505(ugt8)

CPLA:

122541891 122553344(ugt8)

HOC:	132820409(ugt8) 132834939

LERI:

129700342(ugt8) 129712892

PMRN:

116950943(UGT8)

LRJ:	133354332(UGT8)

BFO:

118417462

BBEL:

109473946

LPIC:

129274651

APLC:

110976680 110983599 110983924

SKO:	100369397 100369825 100369971 100370117 100376206 100377334 100377471 100377621 102800741 102801407 102802771 102802804 102802916 102809864

DER:

6541458

DSI:	Dsimw501_GD10375(Dsim_GD10375)

DPO:

6898026

DPE:

6590768

DMN:

108160067

DMO:	Dmoj_GI11922

DAZ:

108614624

DNV:

108651678

DHE:

111605641

ASTE:

118512076

ACEP:

105619733

CFO:

105255889

LHU:

105673022

PGC:

109853431

OBO:

105284682

TNL:

113504541

SLIU:

111354804

BTAB:

109038550

DPX:	DAPPUDRAFT_49730

HAZT:

108673810 108673814

HRO:	HELRODRAFT_177639 HELRODRAFT_186053

HRF:

124125061

HRJ:

124272888

PMAX:

117314629 117344882

OBI:

106870503

LAK:	106160069 106160895 106164760 106177649 112041410

EPA:	110231822 110231830 110242043 110243724

ATEN:

116308303

ADF:	107331984 107342096

AMIL:

114961539 114967941

PDAM:

113676364

TAD:	TRIADDRAFT_22477 TRIADDRAFT_23179 TRIADDRAFT_2420 TRIADDRAFT_2560 TRIADDRAFT_3259 TRIADDRAFT_33822 TRIADDRAFT_53582 TRIADDRAFT_57718 TRIADDRAFT_62077 TRIADDRAFT_62078 TRIADDRAFT_62405

AQU:	105315706 109581221

MNG:	MNEG_2496 MNEG_4536 MNEG_4537 MNEG_7078

CSL:	COCSUDRAFT_47003

CVR:	CHLNCDRAFT_140490

APRO:

F751_1121

SRE:	PTSG_05600

PIF:	PITG_05764

PSOJ:

PHYSODRAFT_517093

» show all

Reference

1 [PMID:5807218]

Authors

Fujino Y, Nakano M.

Title

Enzymic synthesis of cerebroside from ceramide and uridine diphosphate galactose.

Journal

Biochem J 113:573-5 (1969)
DOI:10.1042/bj1130573

Reference

2 [PMID:5793706]

Authors

Morell P, Radin NS.

Title

Synthesis of cerebroside by brain from uridine diphosphate galactose and ceramide containing hydroxy fatty acid.

Journal

Biochemistry 8:506-12 (1969)
DOI:10.1021/bi00830a008

Reference

3 [PMID:5497154]

Authors

Morell P, Costantino-Ceccarini E, Radin NS

Title

The biosynthesis by brain microsomes of cerebrosides containing nonhydroxy fatty acids.

Journal

Arch Biochem Biophys 141:738-48 (1970)
DOI:10.1016/0003-9861(70)90192-X

Reference

4 [PMID:5090043]

Authors

Basu S, Schultz AM, Basu M, Roseman S.

Title

Enzymatic synthesis of galactocerebroside by a galactosyltransferase from embryonic chicken brain.

Journal

J Biol Chem 246:4272-9 (1971)

Reference

5 [PMID:762114]

Authors

Akanuma H, Kishimoto Y

Title

Synthesis of ceramides and cerebrosides containing both alpha-hydroxy and nonhydroxy fatty acids from lignoceroyl-CoA by rat brain microsomes.

Journal

J Biol Chem 254:1050-60 (1979)

Reference

6 [PMID:7306007]

Authors

Koul O, Jungalwala FB

Title

UDP-galactose:ceramide galactosyltransferase of rat central-nervous-system myelin.

Journal

Biochem J 194:633-7 (1981)
DOI:10.1042/bj1940633

Reference

7 [PMID:7694285]

Authors

Schulte S, Stoffel W

Title

Ceramide UDPgalactosyltransferase from myelinating rat brain: purification, cloning, and expression.

Journal

Proc Natl Acad Sci U S A 90:10265-9 (1993)
DOI:10.1073/pnas.90.21.10265

Sequence

[rno:50555]

Reference

8 [PMID:9748263]

Authors

Sprong H, Kruithof B, Leijendekker R, Slot JW, van Meer G, van der Sluijs P

Title

UDP-galactose:ceramide galactosyltransferase is a class I integral membrane protein of the endoplasmic reticulum.

Journal

J Biol Chem 273:25880-8 (1998)
DOI:10.1074/jbc.273.40.25880

Reference

9 [PMID:15998297]

Authors

Fewou SN, Bussow H, Schaeren-Wiemers N, Vanier MT, Macklin WB, Gieselmann V, Eckhardt M

Title

Reversal of non-hydroxy:alpha-hydroxy galactosylceramide ratio and unstable myelin in transgenic mice overexpressing UDP-galactose:ceramide galactosyltransferase.

Journal

J Neurochem 94:469-81 (2005)
DOI:10.1111/j.1471-4159.2005.03221.x

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.47

IUBMB Enzyme Nomenclature:

2.4.1.47

ExPASy - ENZYME nomenclature database:

2.4.1.47

BRENDA, the Enzyme Database:

2.4.1.47

CAS:	37277-56-8

DBGET integrated database retrieval system