Entry
Name
phosphoglucan, water dikinase;
PWD;
OK1
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with paired acceptors
BRITE hierarchy
Sysname
ATP:phospho-alpha-glucan, water phosphotransferase
Reaction(IUBMB)
ATP + [phospho-alpha-glucan] + H2O = AMP + O-phospho-[phospho-alpha-glucan] + phosphate
Substrate
Product
AMP [CPD:
C00020 ];
O-phospho-[phospho-alpha-glucan];
phosphate [CPD:
C00009 ]
Comment
The enzyme phosphorylates granular starch that has previously been phosphorylated by EC
2.7.9.4 , alpha-glucan, water dikinase; there is no activity with unphosphorylated glucans. It transfers the beta-phosphate of ATP to the phosphoglucan, whereas the gamma-phosphate is transferred to water [1]. In contrast to EC
2.7.9.4 , which phosphorylates glucose groups in glucans on O-6, this enzyme phosphorylates glucose groups in phosphorylated starch on O-3 [2]. The protein phosphorylates itself with the beta-phosphate of ATP, which is then transferred to the glucan [1].
History
EC 2.7.9.5 created 2005
Orthology
K15535 phosphoglucan, water dikinase
Genes
CSAT : 104709431 104736667 104771126
BNA : 106365883 106382536 106382666 106440356 125600783
RSZ : 108807397 108812926 108828079 108837261
GHI : 107890518 107903789 107914720 107942709
HSYR : 120119366 120148829 120175186
LJA : Lj5g3v2302970.1(Lj5g3v2302970.1) Lj5g3v2302970.2(Lj5g3v2302970.2)
CMAX : 111470675 111479317
CMOS : 111439141 111457522
CPEP : 111789616 111799885
PALZ : 118040744 118043907
SSPL : 121803609 121808066
MING : 122069638 122088871
PSOM : 113334380 113358202
DOSA : Os12t0297500-01(Os12g0297500)
TAES : 123088324 123147471 123169971
PVIR : 120665851 120699297
SMO : SELMODRAFT_105597 SELMODRAFT_99378
OLU : OSTLU_32991 OSTLU_87084
OTA : OT_ostta04g04000 OT_ostta08g01310
BPG : Bathy04g01300 Bathy08g03390
MIS : MICPUN_104820(PWD1) MICPUN_104865(PWD3) MICPUN_84680(PWD2)
MPP : MICPUCDRAFT_57936(PWD3-A) MICPUCDRAFT_69641(PWD1)
SMIN : v1.2.022336.t1(symbB.v1.2.022336.t1)
» show all
Taxonomy
Reference
Authors
Kotting O, Pusch K, Tiessen A, Geigenberger P, Steup M, Ritte G
Title
Identification of a novel enzyme required for starch metabolism in Arabidopsis leaves. The phosphoglucan, water dikinase.
Journal
Sequence
Reference
Authors
Ritte G, Heydenreich M, Mahlow S, Haebel S, Kotting O, Steup M.
Title
Phosphorylation of C6- and C3-positions of glucosyl residues in starch is catalysed by distinct dikinases.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 2.7.9.5
ExPASy - ENZYME nomenclature database: 2.7.9.5
BRENDA, the Enzyme Database: 2.7.9.5