KEGG ENZYME: 2.8.3.21

ENZYME: 2.8.3.21

Entry

EC 2.8.3.21                 Enzyme

Name

L-carnitine CoA-transferase;
CaiB;
crotonobetainyl/gamma-butyrobetainyl-CoA:carnitine CoA-transferase

Class

Transferases;
Transferring sulfur-containing groups;
CoA-transferases

Sysname

(E)-4-(trimethylammonio)but-2-enoyl-CoA:L-carnitine CoA-transferase

Reaction(IUBMB)

(1) (E)-4-(trimethylammonio)but-2-enoyl-CoA + L-carnitine = (E)-4-(trimethylammonio)but-2-enoate + L-carnitinyl-CoA [RN:R10643];
(2) 4-trimethylammoniobutanoyl-CoA + L-carnitine = 4-trimethylammoniobutanoate + L-carnitinyl-CoA [RN:R10644]

Reaction(KEGG)

R10643 R10644

Substrate

(E)-4-(trimethylammonio)but-2-enoyl-CoA [CPD:C20748];
L-carnitine [CPD:C00318];
4-trimethylammoniobutanoyl-CoA [CPD:C20749]

Product

(E)-4-(trimethylammonio)but-2-enoate [CPD:C04114];
L-carnitinyl-CoA [CPD:C20750];
4-trimethylammoniobutanoate [CPD:C01181]

Comment

The enzyme is found in gammaproteobacteria such as Proteus sp. and Escherichia coli. It has similar activity with both substrates.

History

EC 2.8.3.21 created 2014

Orthology

K08298

L-carnitine CoA-transferase

Genes

ECO:	b0038(caiB)

ECJ:	JW0037(caiB)

ECD:	ECDH10B_0039(caiB)

EBW:	BWG_0036(caiB)

ECOK:

ECMDS42_0031(caiB)

ECOC:

C3026_00200

ECE:	Z0044(caiB)

ECS:	ECs_0041(caiB)

ECF:	ECH74115_0042(caiB)

ETW:	ECSP_0041(caiB)

ELX:	CDCO157_0040

EOI:	ECO111_0039(caiB)

EOJ:	ECO26_0039(caiB)

EOH:	ECO103_0040(caiB)

ECOO:

ECRM13514_0039(caiB)

ECOH:

ECRM13516_0041(caiB)

ESL:

O3K_21355

ESO:

O3O_04030

ESM:

O3M_21255

ECK:	EC55989_0038(caiB)

ECG:	E2348C_0039(caiB)

EOK:	G2583_0040(caiB)

ELR:	ECO55CA74_00190

ELH:

ETEC_0038

ECW:	EcE24377A_0040(caiB)

EUN:

UMNK88_38

ECP:

ECP_0038

ENA:	ECNA114_0025(caiB)

ECOS:

EC958_0172(caiB)

ECV:	APECO1_1943(caiB)

ECOA:

APECO78_03650

ECX:	EcHS_A0042(caiB)

ECM:	EcSMS35_0039(caiB)

ECY:

ECSE_0039

ECR:	ECIAI1_0040(caiB)

ECQ:	ECED1_0037(caiB)

EUM:	ECUMN_0040(caiB)

ECT:	ECIAI39_0039(caiB)

EOC:	CE10_0039(caiB)

ECI:	UTI89_C0044(caiB)

EIH:	ECOK1_0037(caiB)

ECZ:	ECS88_0041(caiB)

ECC:	c0047(caiB)

ELO:	EC042_0040(caiB)

ELN:	NRG857_00200

ESE:

ECSF_0043

ECL:	EcolC_3617

EKO:	EKO11_3874

EKF:	KO11_00190(caiB)

EAB:	ECABU_c00430(caiB)

EDH:	EcDH1_3561

EDJ:	ECDH1ME8569_0037(caiB)

ELU:	UM146_22970

ELW:	ECW_m0038(caiB)

ELL:	WFL_00190(caiB)

ELC:	i14_0040(caiB)

ELD:	i02_0040(caiB)

ELP:	P12B_c0033(caiB)

ELF:	LF82_0258(caiB)

ECOL:

LY180_00195

ECOI:

ECOPMV1_00039(caiB)

ECOJ:

P423_00190

EFE:	EFER_0046(caiB)

EAL:	EAKF1_ch1384(caiB)

EMA:	C1192_12785

ESZ:	FEM44_14685(caiB)

ERUY:

OSH18_09415(caiB)

STY:	STY0082(caiB)

STT:	t0073(caiB)

SEX:	STBHUCCB_830

SENT:

TY21A_00375

STM:	STM0072(caiB)

SEO:	STM14_0085(caiB)

SEV:	STMMW_00741

SEM:	STMDT12_C00730

SEJ:	STMUK_0073(caiB)

SEF:	UMN798_0080(caiB)

SETU:

STU288_00360

SETC:

CFSAN001921_17060

SENR:

STMDT2_00731(caiB)

SEND:

DT104_00731(caiB)

SENI:

CY43_00355

SEEN:

SE451236_06370

SPT:	SPA0073(caiB)

SEK:

SSPA0069

SPQ:	SPAB_00088

SEI:	SPC_0077(caiB)

SEC:	SCH_0066(caiB)

SEH:	SeHA_C0076

SHB:

SU5_0707

SENH:

CFSAN002069_08870

SEEH:

SEEH1578_09390

SEE:	SNSL254_A0076

SENN:

SN31241_10510

SEW:	SeSA_A0080(caiB)

SEA:	SeAg_B0079

SENS:

Q786_00355

SED:

SeD_A0078

SEG:	SG0075(caiB)

SEL:	SPUL_0077(caiB)

SEGA:

SPUCDC_0077(caiB)

SET:	SEN0073(caiB)

SENA:

AU38_00355

SENO:

AU37_00355

SENV:

AU39_00355

SENQ:

AU40_00390

SENL:

IY59_00370

SENJ:

CFSAN001992_10665

SEEC:

CFSAN002050_06810

SEEB:

SEEB0189_019025

SEEP:

I137_00335

SENB:

BN855_750(caiB)

SENE:

IA1_00365

SENC:

SEET0819_07250

SES:	SARI_02928

SBG:	SBG_0057(caiB)

SBZ:

A464_60

SBV:	N643_00275

SALZ:

EOS98_18880

SFL:	SF0035(caiB)

SFX:	S0037(caiB)

SFV:	SFV_0032(caiB)

SFE:	SFxv_0036(caiB)

SFN:

SFy_0044

SFS:

SFyv_0048

SFT:	NCTC1_00035(caiB)

SDY:	SDY_0060(caiB)

SDZ:	Asd1617_00065

CRO:	ROD_00401(caiB)

CKO:

CKO_03345

CFD:	CFNIH1_09900

CBRA:

A6J81_13210

CWE:	CO701_18220

CYO:	CD187_20360

CPOT:

FOB25_07455(caiB)

CFQ:	C2U38_03520

CSED:

JY391_18145(caiB)

CAMA:

F384_00205

CAF:	AL524_22260

CIF:	AL515_04995

CFAR:

CI104_04645

CIR:	C2U53_04685

CIE:	AN232_26290

CPAR:

CUC49_00185

CTEL:

GBC03_25650(caiB)

CITZ:

E4Z61_13395(caiB)

CARS:

E1B03_04735(caiB)

CIX:	M4I31_19970(caiB)

CIB:	HF677_019725(caiB)

CENS:

P2W74_19335(caiB)

EBT:	EBL_c33150(caiB)

ESC:	Entcl_2476

KLE:	AO703_09855

ASUB:

NLZ15_03195(caiB)

YRE:	HEC60_04860(caiB)

KIN:	AB182_21540

PDZ:	HHA33_24105(caiB)

METY:

MRY16398_47850(caiB)

PSGC:

G163CM_28650(caiB)

TOE:	QMG90_18005(caiB)

EBF:

D782_3831

EBC:	C2U52_03235

EBB:

F652_2815

PMR:	PMI2656(caiB)

PMIB:

BB2000_2647(caiB)

PVL:	AOB99_14515

PVG:	CRN77_06410

PHAU:

PH4a_04755

PROT:

BTA34_14495

PCOL:

F1325_16710(caiB)

PCIB:

F9282_17055(caiB)

PPEE:

I6G31_11895(caiB)

PRG:	RB151_007310(caiB)

PHEI:

NCTC12003_00739(caiB)

PRQ:	CYG50_11990(caiB)

PHAG:

PZ638_03515(caiB)

EIC:	NT01EI_2958

ETR:	ETAE_2662(caiB)

ETD:

ETAF_2400

ETE:

ETEE_0763

ETC:	ETAC_12790

EDW:	QY76_07185

EDL:	AAZ33_14080(caiB)

EHO:	A9798_03960

HAV:	AT03_19295

HPAR:

AL518_07300

OPO:	DSM2777_06090

LPV:	HYN51_05070

PRAG:

EKN56_12485(caiB)

LRI:	NCTC12151_02503(caiB)

SLO:

Shew_2674

SSE:

Ssed_3227

SPL:

Spea_4036

SHL:

Shal_0222

SWP:

swp_4936

SALG:

BS332_05365

SLJ:	EGC82_18070

SCAA:

TUM17387_35770(caiB)

SCHK:

GII14_20240(caiB)

SAEG:

K0H80_13655(caiB)

SSPA:

K0I31_13900(caiB)

SHNS:

K0J45_13745(caiB)

SRHS:

K0I63_13830(caiB)

CPIN:

CPIN18020_0492(caiB)

CRX:	CRECT_1850(caiB)

AHS:	AHALO_2625(caiB)

AMAR:

AMRN_2724(caiB)

PBIZ:

LWC08_01455(caiB)

BACI:

B1NLA3E_21280

DSY:	DSY0941 DSY4297 DSY4305

DHD:	Dhaf_0307 Dhaf_1018 Dhaf_1026 Dhaf_4597 Dhaf_4607

DDH:	Desde_0936 Desde_0944

DMT:	DESME_10090 DESME_10120

DRM:

Dred_0571

CSCI:

HDCHBGLK_01434(baiF)

CBIL:

EUBC25_24880

PHX:	KGNDJEFE_00405(baiF)

ELE:	Elen_1833 Elen_2883 Elen_2886

EYY:	EGYY_03120(CaiB) EGYY_03150(CaiB) EGYY_17900(CaiB) EGYY_17980(CaiB) EGYY_27330(CaiB) EGYY_27410(CaiB)

EGD:	GS424_010365(caiB) GS424_016620 GS424_016635

GPA:

GPA_25080

AHAT:

ADCFC_03550 ADCFC_03590 ADCFC_16300

CBAC:

JI75_07135

BWA:	HLV38_02050(caiB)

RTS:	CE91St31_10340

GOR:

KTR9_0720

XYL:	ET495_16525(caiB)

CELC:

K5O09_10665(caiB)

OEK:	FFI11_010745(caiB)

GYU:	FE374_07890(caiB)

PFR:	PFREUD_02430(caiB)

PFRE:

RM25_0221

AHE:

Arch_1362

ARCA:

HC352_07035(caiB)

TPY:	CQ11_04200

TPYO:

X956_03585

TPEC:

HLG82_02700(caiB)

TBER:

QPC17_02460(caiB)

ACTT:

DDD63_04070

AMY:	ADJ76_00880

FSL:	EJO69_02445

WNE:	PIG85_00165(caiB)

PHON:

BH719_03995

» show all

Reference

1 [PMID:11409545]

Authors

Engemann C, Elssner T, Kleber HP

Title

Biotransformation of crotonobetaine to L(-)-carnitine in Proteus sp.

Journal

Arch Microbiol 175:353-9 (2001)
DOI:10.1007/s002030100272

Sequence

[ag:CAD48580] [pmib:BB2000_2647] [pmr:PMI2656]

Reference

2 [PMID:11551212]

Authors

Elssner T, Engemann C, Baumgart K, Kleber HP

Title

Involvement of coenzyme A esters and two new enzymes, an enoyl-CoA hydratase and a CoA-transferase, in the hydration of crotonobetaine to L-carnitine by Escherichia coli.

Journal

Biochemistry 40:11140-8 (2001)
DOI:10.1021/bi0108812

Sequence

[eco:b0038]

Reference

3 [PMID:15518548]

Authors

Stenmark P, Gurmu D, Nordlund P

Title

Crystal structure of CaiB, a type-III CoA transferase in carnitine metabolism.

Journal

Biochemistry 43:13996-4003 (2004)
DOI:10.1021/bi048481c

Sequence

[eco:b0038]

Reference

4 [PMID:15731894]

Authors

Engemann C, Elssner T, Pfeifer S, Krumbholz C, Maier T, Kleber HP

Title

Identification and functional characterisation of genes and corresponding enzymes involved in carnitine metabolism of Proteus sp.

Journal

Arch Microbiol 183:176-89 (2005)
DOI:10.1007/s00203-005-0760-2

Sequence

[ag:CAD48580] [pmib:BB2000_2647] [pmr:PMI2656]

Reference

5 [PMID:15823031]

Authors

Rangarajan ES, Li Y, Iannuzzi P, Cygler M, Matte A

Title

Crystal structure of Escherichia coli crotonobetainyl-CoA: carnitine CoA-transferase (CaiB) and its complexes with CoA and carnitinyl-CoA.

Journal

Biochemistry 44:5728-38 (2005)
DOI:10.1021/bi047656f

Sequence

[eco:b0038]

Other DBs

ExplorEnz - The Enzyme Database:

2.8.3.21

IUBMB Enzyme Nomenclature:

2.8.3.21

ExPASy - ENZYME nomenclature database:

2.8.3.21

BRENDA, the Enzyme Database:

2.8.3.21

DBGET integrated database retrieval system