KEGG   ENZYME: 3.1.4.50
Entry
EC 3.1.4.50                 Enzyme                                 
Name
glycosylphosphatidylinositol phospholipase D;
GPI-PLD;
glycoprotein phospholipase D;
phosphatidylinositol phospholipase D;
phosphatidylinositol-specific phospholipase D
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases
Sysname
glycoprotein-phosphatidylinositol phosphatidohydrolase
Reaction(IUBMB)
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + 3-sn-phosphatidate [RN:R06623]
Reaction(KEGG)
R06623(G);
(other) R04149 R07398
Substrate
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol [CPD:C04248];
H2O [CPD:C00001]
Product
6-(alpha-D-glucosaminyl)-1D-myo-inositol [CPD:C15658];
3-sn-phosphatidate [CPD:C00416]
Comment
This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure. It therefore cleaves proteins from the lipid part of the glycosylphosphatidylinositol (GPI) anchors, but does so by hydrolysis, whereas glycosylphosphatidylinositol diacylglycerol-lyase (EC 4.6.1.14) does so by elimination. It acts on plasma membranes only after solubilization of the substrate with detergents or solvents, but it may act on intracellular membranes.
History
EC 3.1.4.50 created 1990, modified 2002
Pathway
ec00563  Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
ec01100  Metabolic pathways
Orthology
K01127  glycosylphosphatidylinositol phospholipase D
Genes
HSA2822(GPLD1)
PTR462473(GPLD1)
PPS100973223(GPLD1)
GGO101146393(GPLD1)
PON100455517(GPLD1)
NLE100598329(GPLD1)
HMH116459945(GPLD1)
MCC707782(GPLD1)
MCF102125729(GPLD1)
MTHB126952975
MNI105482539(GPLD1)
CSAB103222053(GPLD1)
CATY105572618(GPLD1)
PANU101001857(GPLD1)
TGE112622303(GPLD1)
MLEU105552512(GPLD1)
RRO104663891(GPLD1)
RBB108533667(GPLD1)
TFN117086801(GPLD1)
PTEH111541103(GPLD1)
CANG105526706(GPLD1)
CJC100385911(GPLD1)
SBQ101035773(GPLD1)
CIMI108287071(GPLD1)
CSYR103251843(GPLD1)
MMUR105865209(GPLD1)
LCAT123638333(GPLD1)
PCOQ105805640(GPLD1)
OGA100943003(GPLD1)
MMU14756(Gpld1)
MCAL110307663(Gpld1)
MPAH110333811(Gpld1)
RNO291132(Gpld1)
MCOC116081596(Gpld1)
ANU117713545(Gpld1)
MUN110551065(Gpld1)
CGE100759513(Gpld1)
MAUA101825977(Gpld1)
PROB127229384(Gpld1)
PLEU114700916(Gpld1)
MORG121434002(Gpld1)
MFOT126501644
AAMP119817603(Gpld1)
NGI103745185(Gpld1)
HGL101724062(Gpld1)
CPOC100734898(Gpld1)
CCAN109688371(Gpld1)
DORD106000038(Gpld1)
DSP122111059(Gpld1)
PLOP125352966(Gpld1)
NCAR124988281
MMMA107149294(Gpld1)
OCU100351916
OPI101523140(GPLD1)
TUP102501907(GPLD1)
GVR103596846(GPLD1)
CFA478735(GPLD1)
CLUD112657196(GPLD1)
VVP112920498(GPLD1)
VLG121499732(GPLD1)
NPO129508122(GPLD1)
AML100484842(GPLD1)
UMR103665176(GPLD1)
UAH113264336(GPLD1)
UAR123794261(GPLD1)
ELK111138612
LLV125101848
MPUF101688496(GPLD1)
MNP132017643(GPLD1)
MLK131834816(GPLD1)
NVS122913238(GPLD1)
ORO101370230(GPLD1)
EJU114212585(GPLD1)
ZCA113926556(GPLD1)
MLX118027226(GPLD1)
NSU110586839(GPLD1)
LWW102732488(GPLD1)
FCA101088486(GPLD1)
PYU121020624(GPLD1)
PCOO112853571(GPLD1)
PBG122489168(GPLD1)
LRUF124528799
PTG102964289(GPLD1)
PPAD109252710(GPLD1)
PUC125939406
AJU106968449
HHV120219837(GPLD1)
BTA287025(GPLD1)
BOM102286788(GPLD1)
BIU109577315(GPLD1)
BBUB102389512(GPLD1)
BBIS104991130(GPLD1)
CHX102169718(GPLD1)
OAS101119000(GPLD1)
BTAX128056464(GPLD1)
ODA120870310(GPLD1)
CCAD122429730(GPLD1)
MBEZ129543195(GPLD1)
SSC100157497(GPLD1)
CFR102518271(GPLD1)
CBAI105061677(GPLD1)
CDK105089129(GPLD1)
VPC102538547(GPLD1)
BACU103003582(GPLD1)
LVE103088629(GPLD1)
OOR101288738(GPLD1)
DLE111168561(GPLD1)
PCAD102975868(GPLD1)
PSIU116761576(GPLD1)
NASI112404172(GPLD1)
ECB100067517(GPLD1)
EPZ103546694(GPLD1)
EAI106822357(GPLD1)
MYB102260484(GPLD1)
MYD102753404(GPLD1)
MMYO118672726(GPLD1)
MNA107539689(GPLD1)
PKL118727102(GPLD1)
EFUS103291298(GPLD1)
HAI109396397(GPLD1)
DRO112313946(GPLD1)
SHON118990658(GPLD1)
AJM119054900(GPLD1)
PDIC114489512(GPLD1)
PHAS123833507(GPLD1)
MMF118637507(GPLD1)
RFQ117015438(GPLD1)
PPAM129063916(GPLD1)
PALE102896882(GPLD1)
PGIG120607804(GPLD1)
PVP105306900(GPLD1)
RAY107508879(GPLD1)
MJV108399074(GPLD1)
TOD119230328 119251689(GPLD1)
SARA101538377(GPLD1)
LAV100677378(GPLD1)
TMU101344243
ETF101648291(GPLD1)
DNM101430948(GPLD1)
MDO100024296(GPLD1)
GAS123240054(GPLD1)
SHR100931811(GPLD1)
AFZ127544109
PCW110208720(GPLD1)
OAA100092985(GPLD1)
GGA420819(GPLD1)
PCOC116239798(GPLD1)
MGP100546397(GPLD1)
CJO107309738(GPLD1)
TPAI128087940(GPLD1)
LMUT125690340(GPLD1)
NMEL110394624(GPLD1)
APLA101792700(GPLD1)
ACYG106038351(GPLD1)
CATA118249547(GPLD1)
AFUL116485918(GPLD1)
TGU100230775(GPLD1)
LSR110483464(GPLD1)
SCAN103819395(GPLD1)
PMOA120503505(GPLD1)
OTC121347820(GPLD1)
PRUF121357795(GPLD1)
GFR102032214(GPLD1)
FAB101816045(GPLD1)
OMA130249258(GPLD1)
PHI102114237(GPLD1)
PMAJ107200258(GPLD1)
CCAE111923724(GPLD1)
CCW104691444(GPLD1)
CBRC103613169(GPLD1)
ETL114069829(GPLD1)
ZAB102066942(GPLD1)
ACHL103807301(GPLD1)
SVG106854115(GPLD1)
MMEA130579711(GPLD1)
HRT120750084(GPLD1)
FPG101912228(GPLD1)
FCH102052413(GPLD1)
CLV102090900(GPLD1)
EGZ104125477(GPLD1)
NNI104021793(GPLD1)
PCRI104029539(GPLD1)
PLET104618061(GPLD1)
PCAO104041515(GPLD1)
ACUN113476037(GPLD1)
TALA104368490(GPLD1)
PADL103919061(GPLD1)
AFOR103903819(GPLD1)
ACHC115353059(GPLD1)
HALD104313504(GPLD1)
HLE104829910(GPLD1)
AGEN126048542
GCL127013027
CCRI104167493(GPLD1)
CSTI104554877(GPLD1)
CMAC104478271(GPLD1)
MUI104543338(GPLD1)
BREG104642207(GPLD1)
FGA104078730(GPLD1)
GSTE104254087(GPLD1)
LDI104350256(GPLD1)
MNB103771284(GPLD1)
OHA104335531(GPLD1)
NNT104402944
SHAB115605387(GPLD1)
DPUB104302368(GPLD1)
PGUU104457898(GPLD1)
ACAR104521825(GPLD1)
CPEA104394880(GPLD1)
AVIT104267477(GPLD1)
CVF104288417(GPLD1)
RTD128905130(GPLD1)
CUCA104062174(GPLD1)
TEO104383487(GPLD1)
BRHI104495115(GPLD1)
AAM106483272(GPLD1)
AROW112972795(GPLD1)
NPD112958299 112958300
TGT104564647(GPLD1)
DNE112992496(GPLD1)
SCAM104153090(GPLD1)
ASN102375814(GPLD1)
AMJ102574726(GPLD1)
CPOO109316301(GPLD1)
GGN109302143(GPLD1)
PSS102448848(GPLD1)
CMY102944962(GPLD1)
CCAY125632201(GPLD1)
DCC119852264(GPLD1)
CPIC101945760(GPLD1)
TST117873259(GPLD1)
CABI116836102(GPLD1)
MRV120396443(GPLD1)
ACS100567850(gpld1)
ASAO132774639(GPLD1)
PVT110072878(GPLD1)
SUND121927761(GPLD1)
PBI103063314(GPLD1)
PMUR107289603(GPLD1)
CTIG120300001(GPLD1)
PGUT117672545(GPLD1)
APRI131195166(GPLD1)
PTEX113435583(GPLD1)
NSS113413590(GPLD1)
VKO123021649(GPLD1)
PMUA114600839(GPLD1)
PRAF128417707(GPLD1)
ZVI118090549(GPLD1)
HCG128323558(GPLD1)
GJA107113400(GPLD1)
STOW125439160(GPLD1)
EMC129333212(GPLD1)
XLA108718535
XTR116411679(gpld1)
NPR108792898(GPLD1)
RTEM120940771(GPLD1)
BBUF121001703(GPLD1)
BGAR122939268(GPLD1)
MUO115456470(GPLD1)
GSH117355635(GPLD1)
LCM102345190(GPLD1)
BFO118405295
BBEL109466135
SPU115926364 590298
LPIC129254944
APLC110975439
AJC117117987
SKO102801774
EAF111713084
PCAN112573356
BGT106061229
GAE121374135
HRF124113065
HRJ124288674
CRG105347431 105347449 105347450
CVN111107846 111118101 111118284 111118285 111118286 111119154 111119995 111120355 111120568 111121275
CANU128165758 128165906 128166993
OED125668748 125668749 125669462
MYI110456981
PMAX117326416 117326417
RPHI132760121
DPOL127843817
LAK106165615
NVE5525170
EPA110241955
ATEN116291696
ADF107355065
PDAM113678713
SPIS111330701
DGT114523470
XEN124446939
HSY130636402
SCEYJL132W
SPAOSPAR_J00780
KLAKLLA0_E15401g
KMXKLMA_60039
LTHKLTH0F05830g
ZROZYRO0D03256g
CGRCAGL0L05720g
NCSNCAS_0A09790(NCAS0A09790)
NDINDAI_0G06190(NDAI0G06190)
TBLTBLA_0D05660(TBLA0D05660)
TGBHG536_0F01060
TDLTDEL_0H01030(TDEL0H01030)
KNGKNAG_0C02670(KNAG0C02670)
ZMKHG535_0C00920
PPAPAS_chr4_0595
YLIYALI0B15972g
SLBAWJ20_2030
PKZC5L36_0C01010
BNNFOA43_004168
OPAHPODL_01688
TMLGSTUM_00002772001
SREPTSG_02129
DDIDDB_G0276919(pldG)
DPPDICPUDRAFT_96554
DFADFA_04808(pldG)
ACANACA1_147710
 » show all
Reference
1  [PMID:3422494]
  Authors
Low MG, Prasad AR
  Title
A phospholipase D specific for the phosphatidylinositol anchor of cell-surface proteins is abundant in plasma.
  Journal
Proc Natl Acad Sci U S A 85:980-4 (1988)
DOI:10.1073/pnas.85.4.980
Reference
2  [PMID:3028377]
  Authors
Malik AS, Low MG.
  Title
Conversion of human placental alkaline phosphatase from a high Mr form to a low Mr form during butanol extraction. An investigation of the role of endogenous phosphoinositide-specific phospholipases.
  Journal
Biochem J 240:519-27 (1986)
DOI:10.1042/bj2400519
Reference
3  [PMID:7961859]
  Authors
Li JY, Hollfelder K, Huang KS, Low MG.
  Title
Structural features of GPI-specific phospholipase D revealed by proteolytic fragmentation and Ca2+ binding studies.
  Journal
J Biol Chem 269:28963-71 (1994)
Reference
4  [PMID:11254757]
  Authors
Deeg MA, Bierman EL, Cheung MC.
  Title
GPI-specific phospholipase D associates with an apoA-I- and apoA-IV-containing complex.
  Journal
J Lipid Res 42:442-51 (2001)
Other DBs
ExplorEnz - The Enzyme Database: 3.1.4.50
IUBMB Enzyme Nomenclature: 3.1.4.50
ExPASy - ENZYME nomenclature database: 3.1.4.50
BRENDA, the Enzyme Database: 3.1.4.50
CAS: 113756-14-2

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