Entry
Name
protein-glucosylgalactosylhydroxylysine glucosidase;
PGGHG (gene name);
2-O-alpha-D-glucopyranosyl-5-O-alpha-D-galactopyranosylhydroxy-L-lysine glucohydrolase;
protein-alpha-D-glucosyl-1,2-beta-D-galactosyl-L-hydroxylysine glucohydrolase;
protein-alpha-D-glucosyl-(1->2)-beta-D-galactosyl-L-hydroxylysine glucohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine glucohydrolase
Reaction(IUBMB)
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine + H2O = D-glucose + [collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine [RN:
R04503 ]
Reaction(KEGG)
Substrate
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine;
H2O [CPD:
C00001 ]
Product
D-glucose [CPD:
C00031 ];
[collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine
Comment
The enzyme specifically hydrolyses glucose from alpha-D-glucosyl-(1->2)-beta-D-galactosyl disaccharide units that are linked to hydroxylysine residues of collagen and collagen-like proteins. Acetylation of the epsilon-amino group of the glycosylated hydroxylysine abolishes activity.
History
EC 3.2.1.107 created 1984
Orthology
K22078 protein-glucosylgalactosylhydroxylysine glucosidase
Genes
ZVI : 118078924 118088834(PGGHG)
SRX : 107726850(athl1) 107754371
LCF : 108886949(pgghg) 108891288
BBEL : 109464648 109485036
LPIC : 129258620 129264601
SKO : 100367252 100368173 100368199 100375069 102801557 102807261
DME : Dmel_CG16965(CG16965)
DSI : Dsimw501_GD23801(Dsim_GD23801)
ACOZ : 120957616 120958356 120959147
AARA : 120900865 120901729 120901730
AMER : 121597027 121597088 121597441
ASTE : 118512519 118512520 118512521
AFUN : 125765552 125765553 125765555
AMOU : 128302540 128304671 128304691
AAG : 5564618 5564619 5567690 5576505
AALB : 109417656 109417660 109417661 109417662 109622152 109622154 115255403 115255515
CQU : CpipJ_CPIJ008851 CpipJ_CPIJ008852 CpipJ_CPIJ008853 CpipJ_CPIJ008854 CpipJ_CPIJ008855
CPII : 120424350 120424354 120424366 120424367 120424368
BCOO : 119076367 119078597
HRO : HELRODRAFT_161910 HELRODRAFT_193290 HELRODRAFT_76967
LGI : LOTGIDRAFT_141357 LOTGIDRAFT_226064
PVUL : 126821012 126821019 126821530 126821531 126821532 126821643
CRG : 105324264 105328484 105340679
CVN : 111129258 111131461 111131462
CANU : 128175239 128176475 128177005
OED : 125650079 125651491 125664030
MYI : 110440546 110440547 110440583
PMAX : 117322791 117324382 117324383
RPHI : 132726411 132738791
NVE : 5507997 5508007 5510775 5511304
EPA : 110233900 110233920 110233925 110253303
ATEN : 116290584 116294426 116294435 116294905
ADF : 107342244 107356873 107356887
AMIL : 114956600 114956601 114965960
PDAM : 113668974 113668976 113674506
SPIS : 111321839 111346317 111346319
MBR : MONBRDRAFT_16873 MONBRDRAFT_22300
ACAN : ACA1_054500 ACA1_368900
NGR : NAEGRDRAFT_69774(AM32)
» show all
Taxonomy
Reference
Authors
Hamazaki H, Hotta K.
Title
Purification and characterization of an alpha-glucosidase specific for hydroxylysine-linked disaccharide of collagen.
Journal
J Biol Chem 254:9682-7 (1979)
Reference
Authors
Hamazaki H, Hotta K.
Title
Enzymatic hydrolysis of disaccharide unit of collagen. Isolation of 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosyl-hydroxylysine glucohydrolase from rat spleens.
Journal
Reference
Authors
Sternberg M, Spiro RG.
Title
Studies on the catabolism of the hydroxylysine-linked disaccharide units of basement membranes and collagens. Isolation and characterization of a rat kidney alpha-glucosidase of high specificity.
Journal
J Biol Chem 254:10329-36 (1979)
Reference
Authors
Hamazaki H, Hamazaki MH
Title
Catalytic site of human protein-glucosylgalactosylhydroxylysine glucosidase: Three crucial carboxyl residues were determined by cloning and site-directed mutagenesis.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 3.2.1.107