KEGG ENZYME: 3.2.1.161

ENZYME: 3.2.1.161

Entry

EC 3.2.1.161                Enzyme

Name

beta-apiosyl-beta-glucosidase;
isoflavonoid-7-O-beta[D-apiosyl-(1->6)-beta-D-glucoside] disaccharidase;
isoflavonoid 7-O-beta-apiosyl-glucoside beta-glucosidase;
furcatin hydrolase

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

7-[beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranosyloxy]isoflavonoid beta-D-apiofuranosyl-(1->6)-D-glucohydrolase

Reaction(IUBMB)

7-[beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranosyloxy]isoflavonoid + H2O = a 7-hydroxyisoflavonoid + beta-D-apiofuranosyl-(1->6)-D-glucose

Substrate

7-[beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranosyloxy]isoflavonoid;
H2O [CPD:C00001]

Product

7-hydroxyisoflavonoid;
beta-D-apiofuranosyl-(1->6)-D-glucose

Comment

The enzyme from the tropical tree Dalbergia nigrescens Kurz belongs in glycosyl hydrolase family 1. The enzyme removes disaccharides from the natural substrates dalpatein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside and 7-hydroxy-2',4',5',6-tetramethoxy-7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside (dalnigrein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside) although it can also remove a single glucose residue from isoflavonoid 7-O-glucosides [2]. Daidzin and genistin are also substrates.

History

EC 3.2.1.161 created 2006

Orthology

K20852

beta-apiosyl-beta-glucosidase

Reference

1 [PMID:240725]

Authors

Hosel W, Barz W.

Title

Beta-Glucosidases from Cicer arietinum L. Purification and Properties of isoflavone-7-O-glucoside-specific beta-glucosidases.

Journal

Eur J Biochem 57:607-16 (1975)
DOI:10.1111/j.1432-1033.1975.tb02336.x

Reference

2 [PMID:16098548]

Authors

Chuankhayan P, Hua Y, Svasti J, Sakdarat S, Sullivan PA, Ketudat Cairns JR.

Title

Purification of an isoflavonoid 7-O-beta-apiosyl-glucoside beta-glycosidase and its substrates from Dalbergia nigrescens Kurz.

Journal

Phytochemistry 66:1880-9 (2005)
DOI:10.1016/j.phytochem.2005.06.024

Reference

3 [PMID:14976214]

Authors

Ahn YO, Mizutani M, Saino H, Sakata K

Title

Furcatin hydrolase from Viburnum furcatum Blume is a novel disaccharide-specific acuminosidase in glycosyl hydrolase family 1.

Journal

J Biol Chem 279:23405-14 (2004)
DOI:10.1074/jbc.M311379200

Sequence

[ag:BAD14925]

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.161

IUBMB Enzyme Nomenclature:

3.2.1.161

ExPASy - ENZYME nomenclature database:

3.2.1.161

BRENDA, the Enzyme Database:

3.2.1.161

CAS:	1000598-83-3

All links

Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (13)   
   UniProt (7)   
   SWISS-PROT (2)   
   RefSeq(pep) (4)   
DNA sequence (9)   
   RefSeq(nuc) (8)   
   GenBank (1)   
Protein domain (3)   
   InterPro (3)   
All databases (27)   

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