KEGG ENZYME: 3.2.1.167

ENZYME: 3.2.1.167

Entry

EC 3.2.1.167                Enzyme

Name

baicalin-beta-D-glucuronidase;
baicalinase

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

5,6,7-trihydroxyflavone-7-O-beta-D-glucupyranosiduronate glucuronosylhydrolase

Reaction(IUBMB)

baicalin + H2O = baicalein + D-glucuronate [RN:R09533]

Reaction(KEGG)

R09533

Substrate

baicalin [CPD:C10025];
H2O [CPD:C00001]

Product

baicalein [CPD:C10023];
D-glucuronate [CPD:C00191]

Comment

The enzyme also hydrolyses wogonin 7-O-beta-D-glucuronide and oroxylin 7-O-beta-D-glucuronide with lower efficiency [4]. Neglegible activity with p-nitrophenyl-beta-D-glucuronide [2].

History

EC 3.2.1.167 created 2011

Orthology

K20833

baicalin-beta-D-glucuronidase

Reference

1 [PMID:8593473]

Authors

Ikegami F, Matsunae K, Hisamitsu M, Kurihara T, Yamamoto T, Murakoshi I

Title

Purification and properties of a plant beta-D-glucuronidase form Scutellaria root.

Journal

Biol Pharm Bull 18:1531-4 (1995)
DOI:10.1248/bpb.18.1531

Sequence

[ag:BAA97804]

Reference

Authors

Zhang, C., Zhang, Y., Chen, J. and Liang, X.

Title

Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge.

Journal

Proc Biochem 40:1911-1915 (2005)

Reference

3 [PMID:10858442]

Authors

Sasaki K, Taura F, Shoyama Y, Morimoto S

Title

Molecular characterization of a novel beta-glucuronidase from Scutellaria baicalensis georgi.

Journal

J Biol Chem 275:27466-72 (2000)
DOI:10.1074/jbc.M004674200

Sequence

[ag:BAA97804]

Reference

Authors

Morimoto, S., Harioka, T. and Shoyama, Y.

Title

Purification and characterization of flavone-specific beta-glucuronidase from callus cultures of Scutellaria baicalensis Georgi.

Journal

Planta 195:535-540 (1995)

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.167

IUBMB Enzyme Nomenclature:

3.2.1.167

ExPASy - ENZYME nomenclature database:

3.2.1.167

BRENDA, the Enzyme Database:

3.2.1.167

All links

Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (2)   
   InterPro (2)   
All databases (13)   

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