KEGG ENZYME: 3.4.21.35

ENZYME: 3.4.21.35

Entry

EC 3.4.21.35                Enzyme

Name

tissue kallikrein;
glandular kallikrein;
pancreatic kallikrein;
submandibular kallikrein;
submaxillary kallikrein;
kidney kallikrein;
urinary kallikrein;
kallikrein;
salivary kallikrein;
kininogenin;
kininogenase;
callicrein;
glumorin;
padreatin;
padutin;
kallidinogenase;
bradykininogenase;
depot-padutin;
urokallikrein;
dilminal D;
onokrein P

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases

Reaction(IUBMB)

Preferential cleavage of Arg! bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met! or Leu!. The rat enzyme is unusual in liberating bradykinin directly from autologous kininogens by cleavage at two Arg! bonds [5]

Comment

Formed from tissue prokallikrein by activation with trypsin. In peptidase family S1 (trypsin family). A large number of tissue kallikrein-related sequences have been reported for rats [16] and mice [7], though fewer seem to exist in other mammals. The few that have been isolated and tested on substrates include mouse gamma-renin (EC 3.4.21.54), submandibular proteinase A [2,15], epidermal growth-factor-binding protein, nerve growth factor gamma-subunit, rat tonin [3,4,9], submaxillary proteinases A and B [10], T-kininogenase [18], kallikreins k7 and k8 [17] and human prostate-specific antigen (gamma-seminoprotein, [6])

History

EC 3.4.21.35 created 1965 as EC 3.4.4.21, transferred 1972 to EC 3.4.21.8, part transferred 1981 to EC 3.4.21.35

Orthology

K01325

tissue kallikrein

Genes

HSA:	3816(KLK1) 3817(KLK2)

PTR:	107969791(KLK1) 748637(KLK2)

PPS:	100967412(KLK2) 100995152(KLK1)

GGO:	101144843(KLK1)

PON:	100431610(KLK1) 100432729(KLK2)

NLE:	115836983(KLK1)

HMH:	116478138(KLK1)

MCC:	106994826(KLK1) 719436

MCF:

102135781

MTHB:

126943132 126943136

MNI:	105497139 105497145(KLK1)

CSAB:

103235100(KLK1) 103235106(KLK2)

CATY:

105595342(KLK1) 105595344

PANU:

101004790(KLK1) 101006181

TGE:	112612653 112613504(KLK2)

MLEU:

105528940(KLK2) 105528944(KLK1)

RRO:	104667242(KLK1)

RBB:	108532416(KLK1)

TFN:	117091647(KLK1)

PTEH:

111519950(KLK1) 111547903

CANG:

105514726(KLK1)

CJC:	100402463(KLK1) 100402826

SBQ:	101045013(KLK1) 101045648

CIMI:

108291947 108291980(KLK1) 108291981

CSYR:

103274723 103274726

MMUR:

105865551 105865552 105865567

LCAT:

123623765(KLK1) 123623766

PCOQ:

105809263(KLK1) 105809362

OGA:	100964111 100964738(KLK1)

MMU:	13646(Klk1b22) 13648(Klk1b9) 16612(Klk1) 16613(Klk1b11) 16616(Klk1b21) 16617(Klk1b24) 16618(Klk1b26) 16619(Klk1b27) 16622(Klk1b5) 16623(Klk1b1) 16624(Klk1b8) 18050(Klk1b3)

MCAL:

110288052 110288646 110288648 110288829 110297501 110298249 110298250 110298251 110298252 110298253 110298254 110298255 110298258 110298259 110298262 110298268

MPAH:

110315507 110315664 110315691 110315692 110315693 110318293 110318297 110318311 110318333 110318340 110318349

RNO:	100361452(Klk1c4) 24523(Klk1) 24594(Klk1b3) 24841(Klk1c2) 292855(Klk1c12) 292858(Klk1c10) 292866(Klk1c8) 292868(Klk1c9) 292872(Klk1c3) 408242(Klk1c6)

MCOC:

116100493 116100494 116101214 116101215 116101217 116102109 116102113

ANU:	117715292(Klk1)

MUN:	110539921 110539925

CGE:

100754155

MAUA:

106022386(Klk1)

PROB:

127213897

PLEU:

114684323(Klk1) 114684353

MORG:

121446057(Klk1)

MFOT:

126488198

AAMP:

119802928(Klk1)

NGI:	103743763(Klk1) 103743764

HGL:	101705888 101719001(Klk1)

CPOC:

100716976 100717627 100717905(Klk1)

CCAN:

109683867 109683868 109683884

DORD:

105994168 105994284(Klk1) 105994289 105994292

DSP:	122125573 122125633(Klk1) 122125638 122125650

PLOP:

125338534(Klk1) 125338541 125338543 125368107

NCAR:

124966706 124967234

MMMA:

107151706 107151708(Klk1)

OCU:

100101620

OPI:	105942447(KLK1)

TUP:	102489025(KLK1)

GVR:	103608034(KLK1)

CFA:	403942(KLK1) 403967(KLK2)

CLUD:

112645074(KLK1) 112645075

VVP:	112931184 112931507

VLG:	121475667 121484807

NPO:	129500105 129500627

AML:	100476682 100476932(KLK1) 117799551

UMR:	103657279(KLK1) 103657282

UAH:	113247288(KLK1) 113247344

UAR:	123778021(KLK1) 123778040

ELK:	111160671 111160724

LLV:	125089860 125089862

MPUF:

101690899 101692305(KLK1)

MNP:	132004729 132005283(KLK1)

MLK:	131817584(KLK1) 131817591

NVS:	122913862 122913865(KLK1)

ORO:	101375682 101382562

EJU:	114199586(KLK1) 114199592

ZCA:	113935385(KLK1) 113935397

MLX:	117997969 117998338(KLK1)

NSU:	110572972 110572982 110578655 123323730

LWW:	102751105(KLK1)

FCA:	101090062(KLK1) 101097936

PYU:	121018990(KLK1)

PCOO:

112850833

PBG:	122493807(KLK1)

PVIV:

125152590 125152739

LRUF:

124511698 124511770

PTG:	102963319(KLK1) 102963589

PPAD:

109252991 109252996(KLK1)

PUC:

125916128

AJU:	106989422 106989668

HHV:	120241686 120244551(KLK1)

BTA:	493738(KLK1)

BOM:	102283027(KLK1)

BIU:	109571921(KLK1)

BBUB:

102406481

BBIS:

104995754

CHX:

102177186

OAS:	100048991(KLK1)

BTAX:

128063377

ODA:	120872025(KLK1)

CCAD:

122421576(KLK1)

MBEZ:

129546491(KLK1)

SSC:	431673(KLK1)

CFR:	102515972(KLK1)

CBAI:

105062677

CDK:	105100157(KLK1)

VPC:	102528270(KLK1)

BACU:

103007165(KLK1)

BMUS:

118885121(KLK1) 118904290

LVE:	103081797(KLK1)

OOR:

101283477

DLE:	111181109(KLK1)

PCAD:

102980618

PSIU:

116743839

NASI:

112415494

ECB:	100034023(KLK1E2) 100147008(KLK1) 102147584 111775413

EAI:	106845317 106845494 123275590 123281043

MYB:	102251175(KLK1)

MYD:	102765308(KLK1)

MMYO:

118649263(KLK1)

EFUS:

103297602(KLK1)

MNA:	107531831(KLK1)

DRO:	112310143(KLK1)

AJM:	119052016(KLK1)

PDIC:

114511508(KLK1)

PHAS:

123830267(KLK1)

MMF:	118638349(KLK1)

PPAM:

129082971(KLK1)

HAI:	109390988(KLK1)

RFQ:	117016273 117019016 117035625(KLK1)

PALE:

102893946(KLK1) 102898477

PGIG:

120584017(KLK1)

PVP:	105307422(KLK1)

RAY:	107500469(KLK1)

MJV:	108397931(KLK1)

TOD:	119249652 119249656(KLK1)

SARA:

101548172 101548436 105942741

LAV:	104845473 111749208

TMU:

101344800

ETF:

101656202

DNM:	101416554 101430451 101437536 111766066

OAA:	100087855(KLK1)

CTIG:

120300122

CSAI:

133422322

XHE:

116718752

PRET:

103465468

PFOR:

107833457

PMEI:

106920573

GAF:	122830864 122846929

GFS:

119638812

AMER:

121594399

BCOO:

119068287

FAS:

105266686

AGRG:

126742270 126742273

CCRN:

123293478 123298016

DVT:

126897960

TPAL:

117649754

OSN:

115212877

» show all

Reference

1 [PMID:7043199]

Authors

Fiedler F, Fink E, Tschesche H, Fritz H.

Title

Porcine glandular kallikreins.

Journal

Methods Enzymol 80 Pt C:493-532 (1981)
DOI:10.1016/s0076-6879(81)80042-0

Sequence

[ssc:431673]

Reference

2 [PMID:6295764]

Authors

Anundi H, Ronne H, Peterson PA, Rask L.

Title

Partial amino-acid sequence of the epidermal growth-factor-binding protein.

Journal

Eur J Biochem 129:365-71 (1982)
DOI:10.1111/j.1432-1033.1982.tb07059.x

Reference

3 [PMID:6295383]

Authors

Pesquero JL, Boschcov P, Oliveira MC, Paiva AC.

Title

Effect of substrate size on tonin activity.

Journal

Biochem Biophys Res Commun 108:1441-6 (1982)
DOI:10.1016/S0006-291X(82)80068-5

Reference

4 [PMID:6097352]

Authors

Gutkowska J, Corvol P, Figueiredo AF, Inagami T, Bouhnik J, Genest J

Title

Kinetic studies of rat renin and tonin on purified rat angiotensinogen.

Journal

Can J Biochem Cell Biol 62:137-42 (1984)
DOI:10.1139/o84-020

Reference

5 [PMID:3844939]

Authors

Kato H, Enjyoji K, Miyata T, Hayashi I, Oh-ishi S, Iwanaga S.

Title

Demonstration of arginyl-bradykinin moiety in rat HMW kininogen: direct evidence for liberation of bradykinin by rat glandular kallikreins.

Journal

Biochem Biophys Res Commun 127:289-95 (1985)
DOI:10.1016/S0006-291X(85)80157-1

Reference

6 [PMID:3691800]

Authors

Akiyama K, Nakamura T, Iwanaga S, Hara M.

Title

The chymotrypsin-like activity of human prostate-specific antigen, gamma-seminoprotein.

Journal

FEBS Lett 225:168-72 (1987)
DOI:10.1016/0014-5793(87)81151-1

Reference

7 [PMID:3036794]

Authors

Evans BA, Drinkwater CC, Richards RI.

Title

Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus.

Journal

J Biol Chem 262:8027-34 (1987)

Sequence

[mmu:16623]

Reference

8 [PMID:3643848]

Authors

Fiedler F.

Title

Effects of secondary interactions on the kinetics of peptide and peptide ester hydrolysis by tissue kallikrein and trypsin.

Journal

Eur J Biochem 163:303-12 (1987)
DOI:10.1111/j.1432-1033.1987.tb10801.x

Reference

9 [PMID:2821276]

Authors

Fujinaga M, James MN.

Title

Rat submaxillary gland serine protease, tonin. Structure solution and refinement at 1.8 A resolution.

Journal

J Mol Biol 195:373-96 (1987)
DOI:10.1016/0022-2836(87)90658-9

Sequence

[rno:24841]

Reference

10 [PMID:3482210]

Authors

Kato H, Nakanishi E, Enjyoji K, Hayashi I, Oh-ishi S, Iwanaga S

Title

Characterization of serine proteinases isolated from rat submaxillary gland: with special reference to the degradation of rat kininogens by these enzymes.

Journal

J Biochem 102:1389-404 (1987)
DOI:10.1093/oxfordjournals.jbchem.a122185

Reference

11 [PMID:3237072]

Authors

Bailey GS.

Title

Rat pancreas kallikrein.

Journal

Methods Enzymol 163:115-28 (1988)
DOI:10.1016/0076-6879(88)63013-8

Reference

12 [PMID:2611215]

Authors

Blaber M, Isackson PJ, Marsters JC Jr, Burnier JP, Bradshaw RA.

Title

Substrate specificities of growth factor associated kallikreins of the mouse submandibular gland.

Journal

Biochemistry 28:7813-9 (1989)
DOI:10.1021/bi00445a043

Reference

13 [PMID:3070295]

Authors

Chao J, Chao L.

Title

Rat urinary kallikrein.

Journal

Methods Enzymol 163:128-43 (1988)
DOI:10.1016/0076-6879(88)63014-x

Reference

14 [PMID:2975076]

Authors

Zakrevskaia SF.

Title

[Effectiveness of outpatient dental care for the schoolchildren of Arkhangelsk]

Journal

Stomatologiia (Mosk) 67:62-3 (1988)

Reference

15 [PMID:2523317]

Authors

Bertrand R, Derancourt J, Kassab R.

Title

Selective cleavage at lysine of the 50 kDa-20 kDa connector loop segment of skeletal myosin S-1 by endoproteinase Arg-C.

Journal

FEBS Lett 246:171-6 (1989)
DOI:10.1016/0014-5793(89)80277-7

Reference

16 [PMID:2708383]

Authors

Wines DR, Brady JM, Pritchett DB, Roberts JL, MacDonald RJ.

Title

Organization and expression of the rat kallikrein gene family.

Journal

J Biol Chem 264:7653-62 (1989)

Sequence

[rno:24594 24841]

Reference

17 [PMID:2194829]

Authors

Elmoujahed A, Gutman N, Brillard M, Gauthier F.

Title

Substrate specificity of two kallikrein family gene products isolated from the rat submaxillary gland.

Journal

FEBS Lett 265:137-40 (1990)
DOI:10.1016/0014-5793(90)80903-V

Reference

18 [PMID:2303430]

Authors

Xiong W, Chen LM, Chao J.

Title

Purification and characterization of a kallikrein-like T-kininogenase.

Journal

J Biol Chem 265:2822-7 (1990)

Sequence

[rno:292858]

Other DBs

ExplorEnz - The Enzyme Database:

3.4.21.35

IUBMB Enzyme Nomenclature:

3.4.21.35

ExPASy - ENZYME nomenclature database:

3.4.21.35

BRENDA, the Enzyme Database:

3.4.21.35

CAS:	389069-73-2

DBGET integrated database retrieval system