KEGG ENZYME: 3.4.21.50

ENZYME: 3.4.21.50

Entry

EC 3.4.21.50                Enzyme

Name

lysyl endopeptidase;
Achromobacter proteinase I (also see Comment);
Achromobacter lyticus alkaline proteinase I;
protease I;
achromopeptidase;
lysyl bond specific proteinase

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases

Reaction(IUBMB)

Preferential cleavage: Lys!, including -Lys!Pro-

Comment

From Achromobacter lyticus [6]. Enzymes with similar specificity are produced by Lysobacter enzymogenes (Endoproteinase Lys-C; [3]) and Pseudomonas aeruginosa (Ps-1; [4]). In peptidase family S1 (trypsin family)

History

EC 3.4.21.50 created 1983

Orthology

K01337

lysyl endopeptidase

Genes

XCC:

XCC2821

XCB:

XC_1291

XCA:	xcc-b100_1339

XCP:

XCR_3194

XCV:

XCV3140

XAX:

XACM_2926

XAC:

XAC2992

XCI:	XCAW_03271

XCT:	J151_03177

XCJ:	J158_03156

XCU:	J159_03152

XCN:	J169_03175

XCW:	J162_03156

XCR:	J163_03152

XCM:	J164_03152

XCF:	J172_03168

XFU:	XFF4834R_chr16390 XFF4834R_chr16400

XAO:	XAC29_15215

XOM:	XOO1164(XOO1164) XOO3237(XOO3237)

XOO:	XOO1266 XOO3436

XOP:	PXO_02094 PXO_02206

XOY:	AZ54_06620 AZ54_18155

XOR:	XOC_1253 XOC_1419

XOZ:	BE73_16295

XAL:

XALC_2516

XSA:	SB85_06385

XTN:	FD63_06025 FD63_06030

XFR:	BER92_14610

XVE:	BJD12_05850

XPE:	BJD13_01660

XHR:	XJ27_05695

XGA:	BI317_11035

XVA:	C7V42_06405

XAR:	XB05_09695

XHY:	FZ025_21120

XCZ:	EBN15_01780 EBN15_12945

XEU:	XSP_002908

XHD:	LMG31886_13660(prpL)

XPR:	MUG10_03840 MUG10_16905

XAG:	HEP73_02945

XDY:	NYR95_07920

XAS:	HEP74_02776

PMEX:

H4W19_00845

PJP:	LAG73_11465

LAB:	LA76x_0205 LA76x_1946(prpL) LA76x_3979(lieA) LA76x_3980(lieA)

LAQ:	GLA29479_1560(prpL) GLA29479_3276 GLA29479_3571(lieA) GLA29479_3572(lieA)

LCP:	LC55x_0633 LC55x_1897(prpL) LC55x_2732 LC55x_4007(lieA) LC55x_4008(lieA) LC55x_4945 LC55x_5147

LGU:	LG3211_0894(prpL) LG3211_2066(prpL) LG3211_2609 LG3211_3817 LG3211_3819 LG3211_5119

LEZ:	GLE_0165 GLE_2002 GLE_3341 GLE_3841 GLE_3843 GLE_4756

LEM:	LEN_1307(lyepB) LEN_1308(lyepA) LEN_1765 LEN_4452 LEN_4781

LMB:	C9I47_0198

LYA:	RDV84_09505 RDV84_13725 RDV84_13730 RDV84_13735 RDV84_15990 RDV84_22115

LUM:	CNR27_06010

LUS:	E5843_11635

XBC:	ELE36_10695

LANH:

KR767_16480

XBA:	C7S18_03500 C7S18_12365

TAMN:

N4264_13925 N4264_15755 N4264_16620 N4264_24495

PAE:	PA4175(piv)

PAEV:

N297_4307(prpL)

PAEI:

N296_4307(prpL)

PAU:	PA14_09900(prpL)

PAP:	PSPA7_0919

PAG:	PLES_07521(piv)

PAF:	PAM18_0762(piv)

PNC:	NCGM2_5378(prpL)

PAEB:

NCGM1900_0781(prpL)

PSG:	G655_03830

PRP:	M062_22005

PAEP:

PA1S_03945

PAER:

PA1R_gp2066

PAEM:

U769_03975

PAEL:

T223_03880

PAES:

SCV20265_0789

PAEU:

BN889_04642(piv_1)

PAEG:

AI22_29670

PAEC:

M802_4305(prpL)

PAEO:

M801_4173(prpL)

PPAA:

B7D75_03855(piv)

PKH:	JLK41_03115

SSE:

Ssed_3637

SWD:

Swoo_0115

THT:	E2K93_04820

TACT:

SG35_030845

TVD:	SG34_030675

MCA:

MCA1951

METU:

GNH96_08235

MMEO:

OOT43_05375

THIS:

HZT40_19230 HZT40_19540

TLO:	J9253_14415

TSB:	HMY34_10200

TWN:	L2Y54_03490

TLC:	RCF98_08230

TDU:	QJT80_02905

TVI:	Thivi_1890

TTP:	E6P07_12535

CJAP:

GWK36_12080

THIM:

KFB96_10210 KFB96_14125

WMA:	WM2015_2960

NCU:	F0U83_06470

SOK:	D0B54_16845 D0B54_16850

SINI:

GT972_03695 GT972_03700 GT972_05405 GT972_13365

FCE:	JN531_016000

RHY:	RD110_17425

AJS:

Ajs_3699

ACRA:

BSY15_1975

ACID:

CBP33_02290

ACIP:

CBP36_02695

ACIN:

CBP34_02255

ACIS:

CBP35_16245

ACIO:

EAG14_19090

ARAD:

KI609_02965

ATEM:

PQV96_17965

AAV:	Aave_4238 Aave_4239

AAA:	Acav_4121 Acav_4122

AMON:

H9L24_17930

VEI:

Veis_4069

DAC:

Daci_5718

DEL:	DelCs14_0883

DTS:	BI380_26460

DHK:	BO996_26750

DLA:	I6G47_27025

CTT:	CtCNB1_4289

CTES:

O987_25540

CSER:

CCO03_03295

COF:	FOZ74_04935

CODO:

LAD35_08335 LAD35_20725 LAD35_20730

CRJ:	QMY55_21255

ADN:	Alide_0580

ADK:	Alide2_0545

OTO:	ADJ79_08730

OTK:	C6570_00310

OTD:	J1M35_11225 J1M35_17885

HYL:	LPB072_05440

HPSE:

HPF_18255(prpL)

DPY:	BA022_12445

DIH:	G7047_10175 G7047_15590 G7047_19990 G7047_19995 G7047_20000

DAER:

H9K75_01660

DRG:	H9K76_02415 H9K76_02420 H9K76_17895

DIA:	Dtpsy_0494

SIMP:

C6571_09590

MELM:

C7H73_00010

TTW:	LCC91_10460

NIM:

W01_07110

NARC:

NTG6680_2158

URU:	DSM104443_01133 DSM104443_01800 DSM104443_02442 DSM104443_03785

UPL:	DSM104440_01087 DSM104440_01865 DSM104440_03295

BEB:	AEM42_06830

BEBA:

BWI17_08115 BWI17_15030 BWI17_17730 BWI17_21150

SECH:

B18_04020

DALK:

DSCA_52990

MSD:	MYSTI_03395

AGE:	AA314_03135

AVM:	JQX13_39645

SCL:

sce1929

SCU:	SCE1572_11715

PSTI:

SOO65_11150

MIL:

ML5_4263

MICB:

MicB006_5611

MTUA:

CSH63_12535

MSAG:

GCM10017556_01070

MCRA:

ID554_03515

VER:	HUT12_15785

JTL:	M6D93_04165

HAU:

Haur_0489

DPH:	EHF33_16195

SUS:

Acid_7684

PFER:

IRI77_31760

CTM:	Cabther_B0392

CVL:	J8C06_12335

CHLO:

J8C02_14580

SCOR:

J3U87_02140 J3U87_25125

PGI:

PG_0553

PGN:

PGN_1416

PGT:	PGTDC60_1678

PAH:	Poras_0168 Poras_0385 Poras_0894 Poras_1113 Poras_1474

PCRE:

NCTC12858_00690 NCTC12858_01492 NCTC12858_01785 NCTC12858_01799 NCTC12858_01820

PCAG:

NCTC12856_01360

PEND:

LA319_00105 LA319_01130 LA319_03170

PSOE:

CE91St14_01670

PBT:	ING2E5B_0160

PMUC:

ING2E5A_2521

PET:	PEIBARAKI_6490

PSAC:

PSM36_2730

DYS:	G7050_16610

DYH:	G7051_17115

PDI:	BDI_2766 BDI_2829

PARC:

CI960_20065 CI960_20395

PMER:

INE87_02363 INE87_02605

PGOL:

K6V26_19235 K6V26_21045 K6V26_22570

PJO:	LK449_16665 LK449_17785

TFO:	BFO_1179 BFO_2679 BFO_3286

TOH:	BCB71_00095 BCB71_12565 BCB71_12850

COPR:

Cop2CBH44_23910

BLQ:	L21SP5_03258

DORI:

FH5T_20685

DRC:	G0Q07_09220 G0Q07_15650

MCOS:

GM418_02215 GM418_16830

ASX:	CDL62_03290 CDL62_18010

ALKQ:

M9189_05505

MBAS:

ALGA_4424

CBAE:

COR50_07610

CFIL:

MYF79_10985

FLN:

FLA_2775

HHY:	Halhy_4300 Halhy_5601

SGN:

SGRA_2830

PSTY:

BFS30_24785

PEK:	FFJ24_023970

ECHI:

FKX85_06535

HYP:	A0257_11455

CHK:	D4L85_15380

FPSZ:

AMR72_12715

FAT:	DVK85_08260

FALB:

HYN59_07070 HYN59_07075

FAK:	FUA48_01385 FUA48_15175 FUA48_16005

ASL:	Aeqsu_1857

AEV:	EI546_07705

AIC:	JK629_08380

PTQ:	P700755_003887

PSYD:

IMZ30_11900

NDO:

DDD_1941

NOM:	AAT17_09040

NSD:	BST91_09800

NOB:	CW736_02495

NOJ:	EJ995_04750

NUL:	R1T42_13740

NMF:

NMS_0364

WIN:

WPG_3067

WIJ:	BWZ20_01555

TJE:	TJEJU_0424

FEK:	C1H87_16200

AUE:	C5O00_08435

KOS:	KORDIASMS9_04726

KAN:	IMCC3317_18120

AQB:	D1818_18125

AQA:	D1815_05290 D1815_13295

AQD:	D1816_08430 D1816_14500 D1816_19450 D1816_21315

AQE:	NBT05_01430 NBT05_01435 NBT05_05485

MGEL:

G5B37_12615

ALTI:

ALE3EI_0372

MARX:

INR76_01685

MUT:	GVT53_05050

FBE:	FF125_20475

WVI:	Weevi_1526

CIO:	CEQ15_21485

CNK:	EG343_17440

FBO:	J9309_13535

FTE:	Fluta_1500 Fluta_1592

FLU:	CHH17_18370 CHH17_18865

OHO:	Oweho_3531

SHYR:

LA303_11590

SRU:

SRU_1622

SRM:	SRM_01822(prpL)

MOX:

DAMO_3107

» show all

Reference

1 [PMID:6791693]

Authors

Masaki T, Tanabe M, Nakamura K, Soejima M.

Title

Studies on a new proteolytic enzyme from A chromobacter lyticus M497-1. I. Purification and some enzymatic properties.

Journal

Biochim Biophys Acta 660:44-50 (1981)
DOI:10.1016/0005-2744(81)90106-6

Reference

2 [PMID:6168293]

Authors

Masaki T, Fujihashi T, Nakamura K, Soejima M.

Title

Studies on a new proteolytic enzyme from Achromobacter lyticus M497-1. II. specificity and inhibition studies of Achromobacter protease I.

Journal

Biochim Biophys Acta 660:51-5 (1981)
DOI:10.1016/0005-2744(81)90107-8

Reference

3 [PMID:6359954]

Authors

Jekel PA, Weijer WJ, Beintema JJ.

Title

Use of endoproteinase Lys-C from Lysobacter enzymogenes in protein sequence analysis.

Journal

Anal Biochem 134:347-54 (1983)
DOI:10.1016/0003-2697(83)90308-1

Reference

4 [PMID:3090046]

Authors

Elliott BW Jr, Cohen C.

Title

Isolation and characterization of a lysine-specific protease from Pseudomonas aeruginosa.

Journal

J Biol Chem 261:11259-65 (1986)

Reference

5 [PMID:2684982]

Authors

Ohara T, Makino K, Shinagawa H, Nakata A, Norioka S, Sakiyama F

Title

Cloning, nucleotide sequence, and expression of Achromobacter protease I gene.

Journal

J Biol Chem 264:20625-31 (1989)

Sequence

[ag:AAA78946]

Reference

6 [PMID:2492988]

Authors

Tsunasawa S, Masaki T, Hirose M, Soejima M, Sakiyama F

Title

The primary structure and structural characteristics of Achromobacter lyticus protease I, a lysine-specific serine protease.

Journal

J Biol Chem 264:3832-9 (1989)

Sequence

[ag:AAA78946]

Other DBs

ExplorEnz - The Enzyme Database:

3.4.21.50

IUBMB Enzyme Nomenclature:

3.4.21.50

ExPASy - ENZYME nomenclature database:

3.4.21.50

BRENDA, the Enzyme Database:

3.4.21.50

CAS:	123175-82-6

DBGET integrated database retrieval system