Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
Reaction(IUBMB)
Preferential cleavage: Arg!, Lys!, but with more restricted specificity than trypsin
Comment
Occurs as a tetrameric molecule with high affinity for heparin, in mast cell granules. In peptidase family S1 (trypsin family). Not inhibited by alpha1-proteinase inhibitor or alpha2-macroglobulin
Tanaka T, McRae BJ, Cho K, Cook R, Fraki JE, Johnson DA, Powers JC.
Title
Mammalian tissue trypsin-like enzymes. Comparative reactivities of human skin tryptase, human lung tryptase, and bovine trypsin with peptide 4-nitroanilide and thioester substrates.
Cromlish JA, Seidah NG, Marcinkiewicz M, Hamelin J, Johnson DA, Chretien M.
Title
Human pituitary tryptase: molecular forms, NH2-terminal sequence, immunocytochemical localization, and specificity with prohormone and fluorogenic substrates.