Entry
Name
subtilisin;
alcalase;
alcalase 0.6L;
alcalase 2.5L;
ALK-enzyme;
bacillopeptidase A;
bacillopeptidase B;
Bacillus subtilis alkaline proteinase bioprase;
bioprase AL 15;
bioprase APL 30;
colistinase;
(see also comments);
subtilisin J;
subtilisin S41;
subtilisin Sendai;
subtilisin GX;
subtilisin E;
subtilisin BL;
genenase I;
esperase;
maxatase;
thermoase PC 10;
protease XXVII;
thermoase;
superase;
subtilisin DY;
subtilopeptidase;
SP 266;
savinase 8.0L;
savinase 4.0T;
kazusase;
protease VIII;
opticlean;
Bacillus subtilis alkaline proteinase;
protin A 3L;
savinase;
savinase 16.0L;
savinase 32.0 L EX;
orientase 10B;
protease S
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides
Comment
Subtilisin is a serine endopeptidase, type example of peptidase family S8. It contains no cysteine residues (although these are found in homologous enzymes). Species variants include subtilisin BPN' (also subtilisin B, subtilopeptidase B, subtilopeptidase C, Nagarse, Nagarse proteinase, subtilisin Novo, bacterial proteinase Novo) and subtilisin Carlsberg (subtilisin A, subtilopeptidase A, alcalase Novo). Similar enzymes are produced by various Bacillus subtilis strains and other Bacillus species [1,3]
History
EC 3.4.21.62 created 1992 (EC 3.4.21.14 created 1961 as EC 3.4.4.16, transferred 1972 to EC 3.4.21.14, modified 1986, part incorporated 1992)
Orthology
Genes
GAI : IMCC3135_18295(aprE)
RJG : CCGE525_36295 CCGE525_37610
BSY : I653_05205 I653_16335
BSX : C663_1054(aprE) C663_3243(aprX)
BAZ : BAMTA208_04835(aprE)
BPUM : BW16_05260 BW16_11335
BPUS : UP12_02845 UP12_05210 UP12_10600 UP12_18400
BGY : BGLY_1146(apr) BGLY_1970
BXI : BK049_03075 BK049_08985 BK049_16485
BALT : CFN77_05165 CFN77_11185
BAER : BAE_12730 BAE_18345
BSAF : BSL056_05155 BSL056_11625
BACW : QR42_05055 QR42_10555
BZH : NF868_04460 NF868_09465
BAEI : RE735_09290 RE735_14380
BHK : B4U37_08185 B4U37_08190
BCOH : BC6307_03110 BC6307_09490 BC6307_20400
BFX : BC359_19465 BC359_20835 BC359_20895 BC359_20955
BLE : BleG1_0395 BleG1_3277
SHUA : PQ477_04010 PQ477_10340
BPF : BpOF4_09070(subE) BpOF4_12080
BON : A361_05130 A361_05175
CGOT : J1899_03915 J1899_20685
LGY : T479_08835 T479_19860
VIG : BKP57_02100 BKP57_07055
FAR : ABE41_017340 ABE41_019275
PPSR : I6J18_07910 I6J18_17480
SHV : AAT16_04835 AAT16_09650
BBE : BBR47_20780 BBR47_22370
BFM : BP422_05500 BP422_06405
PPQ : PPSQR21_001320(aprE)
PRI : PRIO_0115 PRIO_6716(aprE)
PPSC : EHS13_14370 EHS13_32420
PHC : BBI08_09060 BBI08_16695
PLAY : DNR44_007855 DNR44_016300
NTR : B0W44_11455 B0W44_14375 B0W44_14495
CALH : IJ00_03035 IJ00_14910
FIS : FIS3754_31010 FIS3754_47610
TOS : Theos_0005 Theos_1000
MRE : K649_03615 K649_04905
MSV : Mesil_3207 Mesil_3440
GES : VT84_14990(apr_1) VT84_17225(aprN)
GOG : C1280_02115 C1280_14680
UAM : UABAM_01285 UABAM_04454
PIT : PIN17_0039 PIN17_0041 PIN17_0436
STAE : HNV11_15330 HNV11_19005
RUP : DTQ70_06725 DTQ70_23935
FAE : FAES_2745 FAES_3048(aprE)
FIB : A6C57_18545 A6C57_23130
CJG : NCTC13459_00605(apr)
AVE : Arcve_0834 Arcve_0894
HALX : M0R89_16480 M0R89_16485 M0R89_16490 M0R89_16495
HAXZ : M0R88_09685 M0R88_17075 M0R88_17085 M0R88_17090
HAYC : NGM10_06340 NGM10_14420 NGM10_14430 NGM10_14435 NGM10_14440
HAXY : NGM07_02950 NGM07_02955 NGM07_16990 NGM07_24425
NSAL : HWV07_05120 HWV07_09995
HAAD : MW046_00465 MW046_00730 MW046_00885
HME : HFX_5138(aprE) HFX_5181(aprE)
HBO : Hbor_32310 Hbor_34040
HALM : FCF25_13890 FCF25_13895
HAH : Halar_2141 Halar_2573
NGE : Natgr_3003 Natgr_3703
HRU : Halru_0481 Halru_0482
TAA : NMY3_00666 NMY3_03174
NIU : DSQ19_01525 DSQ19_03065
» show all
Taxonomy
Reference
1
Authors
Ottesen, M. and Svendsen, I.
Title
The subtilisins.
Journal
Methods Enzymol 19:199-215 (1970)
Reference
2
Authors
Markland, F.S. and Smith, E.L.
Title
Subtilisins: primary structure, chemical and physical properties.
Journal
In: Boyer, P.D. (Ed.), The Enzymes, 3rd ed., vol. 3, Academic Press, New York, 1971, p. 561-608.
Reference
Authors
Tang H.
Title
[The use of single-dose total body irradiation before bone marrow transplantation in treatment of leukemia]
Journal
Zhonghua Fang She Xue Za Zhi 16:296-300 (1982)
Reference
Authors
Nedkov P, Oberthur W, Braunitzer G.
Title
Determination of the complete amino-acid sequence of subtilisin DY and its comparison with the primary structures of the subtilisins BPN', Carlsberg and amylosacchariticus.
Journal
Reference
Authors
Ikemura H, Takagi H, Inouye M
Title
Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli.
Journal
J Biol Chem 262:7859-64 (1987)
Sequence
Reference
6
Authors
Polgar, L.
Title
Structure and function of serine proteases.
Journal
In: Neuberger, A. and Brocklehurst, K. (Eds.), New Comprehensive Biochemistry: Hydrolytic Enzymes, vol. 16, Elsevier, Amsterdam, 1987, p. 159-200.
Other DBs
ExPASy - ENZYME nomenclature database: 3.4.21.62