KEGG ENZYME: 3.4.21.66

ENZYME: 3.4.21.66

Entry

EC 3.4.21.66                Enzyme

Name

thermitase;
thermophilic Streptomyces serine proteinase;
Thermoactinomyces vulgaris serine proteinase

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases

Reaction(IUBMB)

Hydrolysis of proteins, including collagen

Comment

A peptidase of family S8 (subtilisin family) from Thermoactinomyces vulgaris containing a single Cys, near the active site His, and inhibited by p-mercuribenzoate. The N-terminal extension of the polypeptide chain relative to subtilisin contributes to Ca2+-binding and the high thermostability. The amino acid composition and properties of the thermostable enzyme from Streptomyces rectus var. proteolyticus (formerly included in EC 3.4.21.14) are closely similar [1,2].

History

EC 3.4.21.66 created 1992

Orthology

K08651

thermitase

Genes

PSU:	Psesu_0863

LMB:	C9I47_1968

LYT:	DWG18_07540

LYJ:	FKV23_06545 FKV23_10485

TAMN:

N4264_07270

VAF:	D1115_18240

KAK:	Kalk_10505

TIG:

THII_2403

AEH:

Mlg_2387

HHA:

Hhal_2368

HNA:	Hneap_1707

HCH:

HCH_03894

HAHE:

ENC22_12950

HAHH:

O5O45_08120

SVA:

SVA_3720

GPB:	HDN1F_21970

BPYR:

ABD05_14670

MTIM:

DIR46_12445

NII:	Nit79A3_3269

ROM:	EI983_14095

SMUC:

JL100_003090

DAT:	HRM2_24400

DOV:	DSCO28_69020

DALK:

DSCA_59550

DLI:	dnl_11830 dnl_43200

CCX:	COCOR_07741

BBAT:

Bdt_1264 Bdt_2481

BBW:

BDW_04405

BBAC:

EP01_16350

BDQ:	CIK05_05405

BDC:	DOE51_05840 DOE51_12655 DOE51_15455

BDZ:	DOM22_03680 DOM22_13550 DOM22_13955

BEX:	A11Q_1293 A11Q_1429 A11Q_1642 A11Q_1951 A11Q_284 A11Q_946

BMX:	BMS_1331 BMS_2097

HAX:	BALOs_0634 BALOs_1851 BALOs_2011

BSTO:

C0V70_06675 C0V70_10385 C0V70_11480

PSTI:

SOO65_18110 SOO65_18495

BAN:

BA_2380

BAR:

GBAA_2380

BAT:

BAS2216

BAH:	BAMEG_2220

BAI:

BAA_2438

BAX:	H9401_2260

BANT:

A16_24130

BANR:

A16R_24400

BANS:

BAPAT_2274

BANH:

HYU01_11845

BANV:

DJ46_1169(apr)

BCE:

BC2316

BCA:

BCE_2410

BCZ:	BCE33L2139

BCR:	BCAH187_A2480

BCB:	BCB4264_A2345

BCU:	BCAH820_2398

BCG:	BCG9842_B2980

BCQ:

BCQ_2306

BCX:

BCA_2447

BAL:	BACI_c23250

BNC:

BCN_2301

BCF:

bcf_11840

BCER:

BCK_23010

BCEF:

BcrFT9_01888

BCY:	Bcer98_2220

BTK:	BT9727_2155

BTL:

BALH_2119

BTB:	BMB171_C2085

BTT:

HD73_2601

BTHR:

YBT1520_12745

BTHI:

BTK_12915

BTC:	CT43_CH2277

BTF:	YBT020_12085

BTM:

MC28_1586

BTG:	BTB_c23960

BTI:

BTG_08260

BTN:	BTF1_09085

BTHT:

H175_328p188 H175_ch2313

BTHU:

YBT1518_01310 YBT1518_02065 YBT1518_12830 YBT1518_34191

BTW:	BF38_3565(apr)

BTHY:

AQ980_18045

BTY:	Btoyo_4942

BMYC:

DJ92_4728

BBY:	CY96_10695

BWD:	CT694_12545

BTRO:

FJR70_07810 FJR70_29730

BPAC:

LMD38_14920

BPAN:

NLJ82_11455

BALU:

QRY64_14130

BHAI:

KJK41_10325

BCAO:

LC087_02135 LC087_04460

BCO:	Bcell_1945 Bcell_2754

BMH:	BMWSH_p10062

BFX:	BC359_08480

BHA:

BH1491

BKW:	BkAM31D_14100

BCL:

ABC1758

BPF:	BpOF4_14395

BDA:	FSZ17_12775

OIH:

OB2598

PCAL:

BV455_02978

PARG:

PspKH34_32350

ACAI:

ISX45_14330

HMN:	HM131_01595 HM131_06185

HLI:

HLI_16300

HNZ:	P9989_01290

HSAN:

MUN89_03775 MUN89_07090

HSHI:

MUO14_13590

HAMY:

MUO15_12730

VIM:	GWK91_08240

FPN:	ABE65_006275 ABE65_018390 ABE65_019505

FAR:	ABE41_007270 ABE41_018575

FEC:	QNH15_04330

PASA:

BAOM_4719

PPSR:

I6J18_15970

AQT:	FN924_16280

PRD:	F7984_12960

POF:	GS400_19585

MDG:	K8L98_21215

SEDD:

ERJ70_06025

RMF:	D5E69_11015

RAZ:	U9J35_11015

BBEV:

BBEV_2552

MSUT:

LC048_00335 LC048_08625

ALKG:

MOJ78_02645 MOJ78_03330 MOJ78_18650

PHJ:	LC071_08460

EACE:

KKI46_06685

BBE:	BBR47_21070

BLR:	BRLA_c014640 BRLA_c033740

BFM:	BP422_05720

BAGR:

BA6348_18550

BRW:	GOP56_00245 GOP56_08980

BCHS:

JNE38_11120 JNE38_20120

BPAB:

PSE45_10395

PJD:	Pjdr2_2248

PPM:	PPSC2_16770(apr)

PPO:	PPM_3387(apr)

PPOL:

X809_32825

PPQ:	PPSQR21_033820

PPOY:

RE92_20325

PMS:	KNP414_04106

PMQ:	PM3016_5549

PMW:

B2K_28265

PTA:	HPL003_25350

PSAB:

PSAB_17580

PDU:	PDUR_20015

PBD:	PBOR_27385

PGM:	PGRAT_24765

POD:	PODO_23515

PAEN:

P40081_29070

PAEF:

R50345_23265

PAEQ:

R50912_25640

PSTE:

PSTEL_19650

PAEA:

R70723_24770

PAEE:

R70331_23625

PAEH:

H70357_26790

PAEJ:

H70737_23325

PBJ:	VN24_19425

PRI:

PRIO_5168

PSON:

JI735_00880

PNP:	IJ22_09440

PXL:	BS614_21700 BS614_24655

PYG:	AWM70_11470

PSWU:

SY83_14845

PDH:	B9T62_24005

PAIH:

ASL14_21490

PVO:	PVOR_11119

PPSC:

EHS13_13040

PALB:

EJC50_13115

PCHI:

PC41400_10790 PC41400_23050

PBK:	Back11_26060

PPRT:

ET464_07600

PBAC:

HUB98_04295 HUB98_08345

PRZ:	GZH47_05850

PLYC:

GXP70_10410

PSOP:

KP014_24170

PTRI:

KDC22_25080

PTJ:	JRJ22_22005

PALR:

HGI30_01955

PUI:	PUW25_12310

PMAH:

PTQ21_24710

PAMY:

P9222_18755

PDY:	QJQ58_18230

PPOG:

QPK24_12210

PAUN:

MJA45_10795

PHK:	SK066_15165

PPAB:

KET34_21385

TUM:	CBW65_02315 CBW65_02320 CBW65_02325 CBW65_02330 CBW65_02335 CBW65_02340 CBW65_16355 CBW65_16810 CBW65_18875

TAB:	CIG75_04325 CIG75_04330 CIG75_04335 CIG75_12615

EFF:	skT53_00730

PRT:	AUC31_15585

PPLA:

BBI15_07000

PLX:	CW734_13040

PMAT:

BBI11_12840

LFB:	C1X05_05205 C1X05_05210 C1X05_05215 C1X05_14610 C1X05_15305

TVU:	AB849_000840 AB849_005595 AB849_009225

KPUL:

GXN76_05715 GXN76_13910

KEB:	GXN75_04180

PABS:

JIR001_03610 JIR001_09320 JIR001_09330 JIR001_29970

EFM:

M7W_220

EDU:

LIU_14385

CLB:	Clo1100_3059

CSQ:

CSCA_2455

CTYK:

CTK_C05950

CFM:	BJL90_00400

CDRK:

B9W14_07405

CTX:	Clo1313_0689

AWO:	Awo_c03440 Awo_c07490 Awo_c18100 Awo_c23480

HSD:

SD1D_0291

AMIJ:

EQM06_06680

ABUT:

Ami103574_09395

AFT:	BBF96_11540

ERM:	EYR00_14890

ERZ:	ER308_20505

EUZ:	DVS28_a1581

CCYC:

SCMU_35160

ART:	Arth_1050 Arth_1052

ARQ:	BWQ92_04810 BWQ92_04815

ACRY:

AC20117_11930

ARTH:

C3B78_05090

ARTY:

AHiyo4_11920

AOD:	Q8Z05_13080

APN:	Asphe3_10920

PSUL:

AU252_05035

PSNI:

NIBR502771_20255

PSEY:

GU243_21495 GU243_21615

PDEL:

JCQ34_05160

POK:	SMD14_05650

TED:	U5C87_08965

AEB:	C6I20_12490

AWN:	NQV15_03055

GOB:

Gobs_2634

SACG:

FDZ84_09845

SVI:	Svir_22270

SESP:

BN6_81520

KPHY:

AOZ06_50775

ACTA:

C1701_12510

ALO:

CRK60299

SACE:

GIY23_21180

STP:	Strop_3040

SAQ:

Sare_3266

AMS:	AMIS_64790 AMIS_6950

ACTN:

L083_1023 L083_1024

AFS:	AFR_04970 AFR_04975

AIH:	Aiant_45890

ASIC:

Q0Z83_105230 Q0Z83_105240

PRY:	Prubr_26450

RXY:

Rxyl_2188

RRD:	RradSPS_0222 RradSPS_1976 RradSPS_3123

RUB:	GBA63_20955

RMAR:

GBA65_03350 GBA65_09385 GBA65_18480 GBA65_18500 GBA65_20250

BALA:

DSM104299_00678 DSM104299_02378

CWO:

Cwoe_0274

PARN:

NBH00_02820 NBH00_07970 NBH00_08600

PBAJ:

LRS13_08805

TSIN:

OXH18_00390

MPK:

VL20_5283

CWA:	CwatDRAFT_2267

CYC:	PCC7424_0552

ANN:	EH233_18225

TOQ:	HCG51_30890

NCN:	BZZ01_15620

RRS:	RoseRS_0664 RoseRS_0678

RCA:

Rcas_0223

CHL:	Chy400_3661

HAU:	Haur_1188 Haur_2156 Haur_2203 Haur_3265 Haur_3542 Haur_5185

CAP:	CLDAP_00780

THUG:

KNN16_09830 KNN16_14630

TRO:

trd_1587

DMR:	Deima_2872

DPD:	Deipe_2811

DFC:

DFI_08065

DEIN:

DAAJ005_17985

FGI:	OP10G_3378

NPY:	NPRO_12070 NPRO_22330

VAB:

WPS_16060

FMR:	Fuma_02040

MRI:	Mal4_01060(prcA)

GES:	VT84_35395

JET:	L3J17_02630

HBS:	IPV69_03235

TAER:

GT409_03895

PFER:

IRI77_28795

BXY:

BXY_34550

BOA:	Bovatus_04574

BCAE:

A4V03_03010

PEO:	AS203_03020

ANF:

AQPE_3745

CFIL:

MYF79_27490

NSO:	NIASO_01750

AGI:	FSB73_01150

PGO:	FSB84_15910

HHY:	Halhy_2718 Halhy_4825 Halhy_5883

SGN:

SGRA_1885

PHE:	Phep_1319 Phep_3748

PCM:	AY601_2980

SPIB:

G8759_27140

SOQ:	LQ777_27610

LBY:

Lbys_3027

DPF:	ON006_15970

FIB:	A6C57_19635

FLI:	Fleli_3875

HQI:	H9L05_09390

HCU:	MUN79_13660

FLL:	EI427_08900

CHK:	D4L85_19290

FKI:	FK004_03205 FK004_03230

FCR:	HYN56_21970

FBC:	FB2170_13978

TTG:	P8625_15220

SALT:

AO058_16605

KOS:	KORDIASMS9_03542(prcA)

AQD:	D1816_15225

ORH:	Ornrh_2152

ORI:	Q785_10880

ESTE:

HNV03_04620

PLT:

Plut_0364

RLO:	GQ464_013845

RBAR:

AWN76_000220 AWN76_013495

CPRV:

CYPRO_2908

TLE:

Tlet_2037

PHY:	AJ81_03835

NTI:	DNFV4_03822

BIH:	BIP78_0310

MIB:	UY43_C0001G0802

MHU:	Mhun_0648 Mhun_1456 Mhun_1944 Mhun_2053 Mhun_2479

MRTJ:

KHC33_00045 KHC33_07500 KHC33_10125 KHC33_11650 KHC33_14100 KHC33_14165

MBG:	BN140_2139

MEMA:

MMAB1_2826

HME:	HFX_0470(aprE)

PSYT:

DSAG12_01024

» show all

Reference

1 [PMID:4711613]

Authors

Mizusawa K, Yoshida F.

Title

Thermophilic Streptomyces alkaline proteinase. II. The role of a sulfhydryl group and the conformational stability.

Journal

J Biol Chem 248:4417-23 (1973)

Reference

2 [PMID:4373436]

Authors

Borgia P, Campbell LL.

Title

Properties of two homologous alkaline proteases from Streptomyces rectus.

Journal

J Bacteriol 120:1109-15 (1974)
DOI:10.1128/JB.120.3.1109-1115.1974

Reference

3 [PMID:7051706]

Authors

Kleine R.

Title

Properties of thermitase, a thermostable serine protease from Thermoactinomyces vulgaris.

Journal

Acta Biol Med Ger 41:89-102 (1982)

Reference

Authors

Meloun, B., Baudy, M., Kostka, V., Hausdorf, G., Frommel, C. and Hohne, W.E.

Title

Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases.

Journal

FEBS Lett 183:195-200 (1985)

Reference

5 [PMID:2196375]

Authors

Teplyakov AV, Kuranova IP, Harutyunyan EH, Vainshtein BK, Frommel C, Hohne WE, Wilson KS.

Title

Crystal structure of thermitase at 1.4 A resolution.

Journal

J Mol Biol 214:261-79 (1990)
DOI:10.1016/0022-2836(90)90160-N

Other DBs

ExplorEnz - The Enzyme Database:

3.4.21.66

IUBMB Enzyme Nomenclature:

3.4.21.66

ExPASy - ENZYME nomenclature database:

3.4.21.66

BRENDA, the Enzyme Database:

3.4.21.66

CAS:	69772-87-8

DBGET integrated database retrieval system