peptidase 1 (mite);
allergen Der f 1;
allergen Der p 1;
antigen Der p 1;
antigen Eur m 1;
antigen Pso o 1;
major mite fecal allergen Der p 1;
Der p 1;
Der f 1;
Eur m 1;
endopeptidase 1 (mite)
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases
Reaction(IUBMB)
Broad endopeptidase specificity
Comment
This enzyme, derived from the house dust mite, is a major component of the allergic immune response [2]. The substrate specificity of this enzyme is not altogether clear. It cleaves the low-affinity IgE receptor CD23 at Glu298!Ser299 and Ser155!Ser156 [1]. It also cleaves the pulmonary structural proteins occludin and claudin at Leu!Leu, Asp!Leu and at Gly!Thr bonds [1,2]. It can also cleave the alpha subunit of the interleukin-2 (IL-2) receptor (CD25) [4]. Using a positional scanning combinatorial library, it was found that the major substrate-specificity determinant is for Ala in the P2 position [3]. The enzyme shows only a slight preference for basic amino acids in the P1 and P3 positions and a preference for aliphatic amino acids such as Ile, Pro, Val, Leu and norleucine in the P4 position [3]. Belongs in peptidase family C1A.
History
EC 3.4.22.65 created 2007
Reference
1
Authors
Meighan, P. and Pirzad, R.
Title
Mite endopeptidase 1.
Journal
In: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. (Eds.), Handbook of Proteolytic Enzymes, 2nd ed., Elsevier, London, 2004, p. 1187-1189.
The house dust mite allergen Der p1 catalytically inactivates alpha 1-antitrypsin by specific reactive centre loop cleavage: a mechanism that promotes airway inflammation and asthma.
Proteolytic cleavage of CD25, the alpha subunit of the human T cell interleukin 2 receptor, by Der p 1, a major mite allergen with cysteine protease activity.
A sensitive fluorescent assay for measuring the cysteine protease activity of Der p 1, a major allergen from the dust mite Dermatophagoides pteronyssinus.
