KEGG   ENZYME: 3.4.23.12
Entry
EC 3.4.23.12                Enzyme                                 
Name
nepenthesin;
Nepenthes aspartic proteinase;
Nepenthes acid proteinase;
nepenthacin;
nepenthasin;
aspartyl endopeptidase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
Reaction(IUBMB)
Similar to pepsin, but also cleaves on either side of Asp and at Lys!Arg
Comment
From the insectivorous plants Nepenthes spp. (secretions) and Drosera peltata (ground-up leaves). Aspartic endopeptidases are probably present in many other plants, including Lotus [3] and sorghum [2]. In peptidase family A1 (pepsin A family)
History
EC 3.4.23.12 created 1972 as EC 3.4.99.4, transferred 1978 to EC 3.4.23.12, modified 1981
Reference
1  [PMID:5354017]
  Authors
Amagase S, Nakayama S, Tsugita A.
  Title
Acid protease in Nepenthes. II. Study on the specificity of nepenthesin.
  Journal
J Biochem (Tokyo) 66:431-9 (1969)
DOI:10.1093/oxfordjournals.jbchem.a129166
Reference
2  [PMID:5486576]
  Authors
Garg GK, Virupaksha TK.
  Title
Acid protease from germinated sorghum. 2. Substrate specificity with synthetic peptides and ribonuclease A.
  Journal
Eur J Biochem 17:13-8 (1970)
DOI:10.1111/j.1432-1033.1970.tb01125.x
Reference
3
  Authors
Shinano, S. and Fukushima, K.
  Title
Studies on lotus seed protease. Part III. Some physicochemical and enzymic properties.
  Journal
Agric Biol Chem 35:1488-1494 (1971)
Reference
4  [PMID:5069751]
  Authors
Amagase S.
  Title
Digestive enzymes in insectivorous plants. 3. Acid proteases in the genus Nepenthes and Drosera peltata.
  Journal
J Biochem (Tokyo) 72:73-81 (1972)
DOI:10.1093/oxfordjournals.jbchem.a129899
Reference
5  [PMID:4436292]
  Authors
Takahashi K, Chang W, Ko J.
  Title
Specific inhibition of acid proteases from brain, kidney, skeletal muscle, and insectivorous plants by diazoacetyl-DL-norleucine methyl ester and by pepstatin.
  Journal
J Biochem (Tokyo) 76:897-9 (1974)
Reference
6  [PMID:16526095]
  Authors
Coiras M, Camafeita E, Urena T, Lopez JA, Caballero F, Fernandez B, Lopez-Huertas MR, Perez-Olmeda M, Alcami J.
  Title
Modifications in the human T cell proteome induced by intracellular HIV-1 Tat protein expression.
  Journal
Proteomics 6 Suppl 1:S63-73 (2006)
DOI:10.1002/pmic.200500437
Other DBs
ExplorEnz - The Enzyme Database: 3.4.23.12
IUBMB Enzyme Nomenclature: 3.4.23.12
ExPASy - ENZYME nomenclature database: 3.4.23.12
BRENDA, the Enzyme Database: 3.4.23.12
CAS: 9073-80-7

  All links  
Protein sequence (317)   
   UniProt (313)   
   SWISS-PROT (4)   
Protein domain (8)   
   InterPro (8)   
All databases (325)   

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