KEGG   ENZYME: 3.4.23.49
Entry
EC 3.4.23.49                Enzyme                                 
Name
omptin;
protease VII;
protease A;
gene ompT proteins;
ompT protease;
protein a;
Pla;
OmpT
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
Reaction(IUBMB)
Has a virtual requirement for Arg in the P1 position and a slightly less stringent preference for this residue in the P1' position, which can also contain Lys, Gly or Val.
Comment
A product of the ompT gene of Escherichia coli, and associated with the outer membrane. Omptin shows a preference for cleavage between consecutive basic amino acids, but is capable of cleavage when P1' is a non-basic residue [5,7]. Belongs in peptidase family A26.
History
EC 3.4.23.49 created 1993 as EC 3.4.21.87, transferred 2006 to EC 3.4.23.49
Orthology
K01355  omptin
Genes
ECO: b0565(ompT)
ECJ: JW0554(ompT)
ECD: ECDH10B_0523(ompT) ECDH10B_0631(ompT)
EBW: BWG_0436(ompT)
ECOC: C3026_02800
ECE: Z1931
ECS: ECs_1663(ompT)
ECF: ECH74115_1653
ETW: ECSP_1567(ompT)
ELX: CDCO157_1594
EOI: ECO111_0586
EOJ: ECO26_0631
EOH: ECO103_0566
ECOO: ECRM13514_0582
ECG: E2348C_0466(ompT)
EOK: G2583_0717(ompT)
ELR: ECO55CA74_03500
ECP: ECP_0597
ECOS: EC958_0673(ompT)
ECV: APECO1_1482(ompT) APECO1_546
ECOA: APECO78_06360
ECM: EcSMS35_A0099(ompT)
ECY: ECSE_0621
EUM: ECUMN_0637(ompT)
ECT: ECIAI39_0539(ompT)
EOC: CE10_0562(ompT)
EBR: ECB_00519(ompT)
ECI: UTI89_C0566(ompT)
EIH: ECOK1_0571(ompT) ECOK1_0575(ompT)
ECZ: ECS88_0597(ompT) ECS88_0602(ompT) ECS88_p0155(ompT)
ECC: c0652(ompT)
ELN: NRG857_02540 NRG857_30283(ompT1)
ESE: ECSF_0496
EAB: ECABU_c06150
EDH: EcDH1_3064
EDJ: ECDH1ME8569_0533(ompT)
ELU: UM146_14685
ELC: i14_0626(ompT)
ELD: i02_0626(ompT)
ELF: LF82_1565(ompT)
ECOI: ECOPMV1_00578(ompT_1) ECOPMV1_00582(ompT_2)
ECOJ: P423_02720
EAL: EAKF1_ch0285 EAKF1_ch0900
ESZ: FEM44_05105 FEM44_13015
SES: SARI_01746
ENL: A3UG_21740
ENF: AKI40_2865
CRO: ROD_20151(ompT)
EBT: EBL_c18290
KCO: BWI95_15415
KOT: EH164_12575
KOB: HF650_10520
KOO: O9K67_13875
YRU: BD65_376(ompT)
EAME: GXP68_13960
EAM: EAMY_0249(ompT1) EAMY_2844(ompT3) EAMY_2848(ompT5)
EAY: EAM_0238 EAM_0740 EAM_0742
EPY: EpC_02590(ompT)
EPR: EPYR_00269(ompT)
ERJ: EJP617_13540(ompT)
ETO: RIN69_12805
PFQ: QQ39_09680
PCG: AXG94_20795
PMED: E3Z27_05160
ABER: BSR55_19170
MESL: KKZ03_21340
SIX: BSY16_2715
EMX: FKV68_24320
AGR: AGROH133_05297
ATF: Ach5_10520
AGT: EYD00_04005
RIR: BN877_I1129
RPUS: CFBP5875_04415
REL: REMIM1_PE00480
RHV: BA939_00570
ROY: G3A56_05140
DEI: C4375_08320
DEA: FPZ08_15605
PPRF: DPRO_2449
 » show all
Reference
1  [PMID:3278297]
  Authors
Grodberg J, Lundrigan MD, Toledo DL, Mangel WF, Dunn JJ
  Title
Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12.
  Journal
Nucleic Acids Res 16:1209 (1988)
DOI:10.1093/nar/16.3.1209
  Sequence
[eco:b0565]
Reference
2  [PMID:3056908]
  Authors
Sugimura K, Nishihara T.
  Title
Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT.
  Journal
J Bacteriol 170:5625-32 (1988)
DOI:10.1128/JB.170.12.5625-5632.1988
  Sequence
[eco:b0565]
Reference
3  [PMID:1603076]
  Authors
Hanke C, Hess J, Schumacher G, Goebel W.
  Title
Processing by OmpT of fusion proteins carrying the HlyA transport signal during secretion by the Escherichia coli hemolysin transport system.
  Journal
Mol Gen Genet 233:42-8 (1992)
DOI:10.1007/bf00587559
Reference
4
  Authors
Dekker, N.
  Title
Omptin.
  Journal
In: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. (Eds.), Handbook of Proteolytic Enzymes, 2nd ed., Elsevier, London, 2004, p. 212-216.
Reference
5  [PMID:11566868]
  Authors
Vandeputte-Rutten L, Kramer RA, Kroon J, Dekker N, Egmond MR, Gros P.
  Title
Crystal structure of the outer membrane protease OmpT from Escherichia coli suggests a novel catalytic site.
  Journal
EMBO J 20:5033-9 (2001)
DOI:10.1093/emboj/20.18.5033
  Sequence
[eco:b0565]
Reference
6  [PMID:11576541]
  Authors
Kramer RA, Vandeputte-Rutten L, de Roon GJ, Gros P, Dekker N, Egmond MR.
  Title
Identification of essential acidic residues of outer membrane protease OmpT supports a novel active site.
  Journal
FEBS Lett 505:426-30 (2001)
DOI:10.1016/S0014-5793(01)02863-0
  Sequence
[eco:b0565]
Reference
7  [PMID:15317797]
  Authors
McCarter JD, Stephens D, Shoemaker K, Rosenberg S, Kirsch JF, Georgiou G.
  Title
Substrate specificity of the Escherichia coli outer membrane protease OmpT.
  Journal
J Bacteriol 186:5919-25 (2004)
DOI:10.1128/JB.186.17.5919-5925.2004
Other DBs
ExplorEnz - The Enzyme Database: 3.4.23.49
IUBMB Enzyme Nomenclature: 3.4.23.49
ExPASy - ENZYME nomenclature database: 3.4.23.49
BRENDA, the Enzyme Database: 3.4.23.49
CAS: 150770-86-8

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