KEGG ENZYME: 3.4.24.28

ENZYME: 3.4.24.28

Entry

EC 3.4.24.28                Enzyme

Name

bacillolysin;
Bacillus metalloendopeptidase;
Bacillus subtilis neutral proteinase;
anilozyme P 10;
Bacillus metalloproteinase;
Bacillus neutral proteinase;
megateriopeptidase

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases

Reaction(IUBMB)

Similar, but not identical, to that of thermolysin

Comment

Variants of this enzyme have been found in species of Bacillus including B. subtilis [1,6], B. amyloliquefaciens [5], B. megaterium (megateriopeptidase, [2]), B. mesentericus [10], B. cereus [3,8,9] and B. stearothermophilus [7]. In peptidase family M4 (thermolysin family). Formerly included in EC 3.4.24.4

History

EC 3.4.24.28 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.28

Orthology

K01400

bacillolysin

Genes

ETR:

ETAE_2022

ETD:

ETAF_1830

ETC:	ETAC_09270

VMI:	AL543_03465

SHL:

Shal_3483

SVO:

SVI_0640

SPSH:

FM037_04605

SAGA:

M5M_09630

HCH:

HCH_00828

HAHE:

ENC22_03390

PKT:	AT984_18215

RHX:	AMK02_PC00026

BGZ:	XH91_29805

AUB:	LXB15_11135

SPHS:

ETR14_02875

AFW:	Anae109_3035

MXA:

MXAN_2754

MSD:	MYSTI_00607 MYSTI_01772 MYSTI_03187

MYM:	A176_004272

MFB:	MFUL124B02_03455 MFUL124B02_09985 MFUL124B02_17365 MFUL124B02_41865

MYX:	QEG98_14650

CCX:	COCOR_00799(npr) COCOR_01296(nprM) COCOR_05687(nprS) COCOR_06712(nprM1)

MFU:	LILAB_21525 LILAB_34165

MMAS:

MYMAC_002728

SUR:	STAUR_1693 STAUR_4897(fibA) STAUR_7158 STAUR_7459

AGE:	AA314_00679 AA314_02845 AA314_04696 AA314_05742 AA314_05743 AA314_08228 AA314_09142

AVM:	JQX13_06110 JQX13_20980 JQX13_22835 JQX13_37405 JQX13_47940

MBD:	MEBOL_000603 MEBOL_003411 MEBOL_004624 MEBOL_007739 MEBOL_007740

CFUS:

CYFUS_000044 CYFUS_000703 CYFUS_002522 CYFUS_003038 CYFUS_004891 CYFUS_005614 CYFUS_009605

SCL:

sce4498

CCRO:

CMC5_042380

PAUU:

E8A73_004680 E8A73_026080 E8A73_026085 E8A73_034725 E8A73_040880

LLU:	AKJ09_05311 AKJ09_06197

BSU:	BSU14700(nprE)

BSR:

I33_1652

BSL:

A7A1_0557

BSH:	BSU6051_14700(nprE)

BSY:	I653_07555

BSUT:

BSUB_01599(nprE)

BSUL:

BSUA_01599(nprE)

BSUS:

Q433_08425

BSO:	BSNT_07973(nprE)

BSN:	BSn5_19435

BSQ:	B657_14700(nprE)

BSX:	C663_1514(nprE)

BSP:	U712_07740

BSS:	BSUW23_07570(nprE)

BST:

GYO_1812

BIT:	BIS30_18175

BAY:	RBAM_014550

BAQ:	BACAU_1430(npr)

BYA:	BANAU_1393(npr)

BAMP:

B938_07575

BQY:	MUS_1567(nprE)

BAML:

BAM5036_1392(nprE)

BAMA:

RBAU_1436(nprE)

BAMN:

BASU_1416(nprE)

BAMB:

BAPNAU_2298(npr)

BAMT:

AJ82_08345

BAMY:

V529_14130(nprE)

BMP:	NG74_01512(npr)

BAO:	BAMF_1546(nprE)

BAZ:	BAMTA208_09795(nprE)

BQL:	LL3_01578(nprE)

BXH:	BAXH7_01996(npr)

BAMI:

KSO_012080

BAMC:

U471_14890

BAMF:

U722_07735

BSIA:

CWD84_13960

BAE:	BATR1942_05125

BVM:	B9C48_07450

BHT:	DIC78_02040

BMOJ:

HC660_15350(nprE)

BTEQ:

G4P54_07695(nprE)

BIQ:	AN935_07680

BSTR:

QI003_07945(nprE)

BAN:

BA_0599

BAR:

GBAA_0599

BAT:

BAS0567

BAH:	BAMEG_3986

BAI:

BAA_0683

BAX:	H9401_0571 H9401_2444

BANT:

A16_06580

BANR:

A16R_06670

BANS:

BAPAT_0578 BAPAT_2463

BANH:

HYU01_03265

BANV:

DJ46_1349 DJ46_5058(npr)

BCE:

BC0602

BCA:

BCE_0667

BCZ:	BCE33L0511(npr) pE33L466_0375(npr)

BCR:	BCAH187_A0727 BCAH187_C0042

BCB:	BCB4264_A0638

BCU:	BCAH820_0656

BCG:	BCG9842_B4700 BCG9842_B5478

BCQ:	BCQ_0665(npr) BCQ_PI139

BCX:	BCA_0632 BCA_3311

BAL:	BACI_c06090(npr1)

BNC:	BCN_0574 BCN_P033

BCF:	bcf_03070 bcf_15970

BCER:

BCK_05145

BCY:	Bcer98_0515 Bcer98_1599 Bcer98_2213

BTK:	BT9727_0509(npr)

BTL:	BALH_0539(nprE) BALH_2915(npr)

BTB:	BMB171_C0524

BTT:

HD73_0673

BTHR:

YBT1520_03330

BTHI:

BTK_03405

BTC:	CT43_CH0522 CT43_CH5391

BTF:	YBT020_03270

BTM:	MC28_5326(npr)

BTG:	BTB_c06140(npr1) BTB_c55530(nprS)

BTI:

BTG_18155

BTN:	BTF1_00720 BTF1_25250

BTHT:

H175_ch0525 H175_ch5482

BTHU:

YBT1518_03175 YBT1518_33971

BTW:	BF38_1848(npr)

BTHY:

AQ980_27615

BWE:	BcerKBAB4_0514

BWW:	bwei_5002(npr)

BMYO:

BG05_5325(npr)

BTY:	Btoyo_3276

BMYC:

DJ92_2128(nprB) DJ92_3204(npr) DJ92_5598(nprM)

BBY:	CY96_02170

BWD:	CT694_03420

BTRO:

FJR70_25225

BMOB:

MLA2C4_03170

BNT:	GSN03_03290

BPAC:

LMD38_23075 LMD38_27735

BPAN:

NLJ82_03225 NLJ82_27195

BALU:

QRY64_05350 QRY64_18545

BIF:	N288_22335

BWH:	A9C19_01495

BBAD:

K7T73_11135

BCAB:

EFK13_08165(nprE)

BRY:	M0696_08025(nprE)

BJS:

MY9_1610

BACP:

SB24_02420

BACB:

OY17_10370

BACY:

QF06_06375

BACL:

BS34A_16300(nprE)

BALM:

BsLM_1565

BACS:

AUL54_03270

BACA:

FAY30_02370 FAY30_05135

BHAI:

KJK41_01910 KJK41_10320 KJK41_12900 KJK41_19665

BCAO:

LC087_09995

BARD:

QRY57_04825

BCOH:

BC6307_24130

BMQ:	BMQ_2326(nprM)

BMD:	BMD_2285(nprM)

BMH:	BMWSH_2914

BMEG:

BG04_4696(nprM)

PKS:	IE339_12435 IE339_20820

BGI:	BGM20_01720

BON:	A361_11180

CFIR:

NAF01_10100

CSPG:

LS684_21610

CPSS:

M5V91_10360

BDA:	FSZ17_06105

GTE:	GTCCBUS3UF5_31940

GTK:	GT3570_13885

GTM:	GT3921_05230

GLI:	GLN3_06885

BCAL:

CWI35_14050

GYC:	GYMC61_0670

GYA:	GYMC52_2884

GCT:	GC56T3_0655

GGH:	GHH_c29140

GEA:	GARCT_02867(nprT)

GEL:	IB49_06060

GSE:	GT50_05725

PARG:

PspKH34_06640(npr)

ANM:	GFC28_3256(npr)

ANL:	GFC29_2178(npr)

AND:	GRQ40_15315

ACAI:

ISX45_12395

HHD:	HBHAL_4199(npr2) HBHAL_4829(npr4)

HMN:	HM131_05795

HNZ:	P9989_08705 P9989_19385 P9989_19920

HSAN:

MUN89_04355

HSHI:

MUO14_10110 MUO14_21605

HAMY:

MUO15_05940

VIR:	X953_07045 X953_16635

VIG:	BKP57_18270

VIL:	CFK37_04370 CFK37_17250

VNE:	CFK40_20585

VPN:	A21D_00581(nprE)

VIM:	GWK91_10385

VPT:	KBP50_11440

FPN:	ABE65_001600 ABE65_005430 ABE65_019890

FAR:	ABE41_001575 ABE41_018950

FEC:	QNH15_06615 QNH15_13415 QNH15_24905

BSJ:	UP17_08785 UP17_22480

BMUR:

ABE28_021070

PBUT:

DTO10_08905

PPSR:

I6J18_08265

PFRI:

L8956_23935

AQT:	FN924_01690

RAX:	KO561_01525

PRD:	F7984_02190

RUE:	DT065_13890

SALE:

EPH95_13830

SCIA:

HUG15_03640

PCHU:

QNI29_18430

NMK:	CHR53_02870 CHR53_05275

NDT:	L1999_03885

NCM:	QNK12_03335

NNV:	QNH39_05250 QNH39_15655

MLIT:

KDJ21_007545

BVQ:	FHE72_02230 FHE72_20120

RAZ:	U9J35_01910 U9J35_11010 U9J35_19440

BVJ:	I5776_18080

GAJ:	MY490_06060 MY490_08035

MJO:	FOF60_01945 FOF60_12185

MSUT:

LC048_10010

ECTO:

MUG87_10505 MUG87_15830

ALKG:

MOJ78_16035

PHJ:	LC071_06515 LC071_15730 LC071_17970

ESI:

Exig_2940

EAT:	EAT1b_0125

EAN:

Eab7_2752

EXM:	U719_16005

EXU:	ESP131_01625

EAP:	KB235_09975

EACE:

KKI46_15870

EAUR:

NMQ00_08605

EMM:	PTI97_06520

EPF:	OE059_08160

BBE:	BBR47_13290(npr)

BLR:	BRLA_c008220

BFM:	BP422_01770

BAGR:

BA6348_24595

BRW:	GOP56_19865

BPAB:

PSE45_04305

PPY:	PPE_03843 PPE_03844

PPM:	PPSC2_20195(npr)

PPO:	PPM_4046(npr)

PPOL:

X809_36325 X809_36330

PPQ:	PPSQR21_040810(lasB)

PPOY:

RE92_17000

PMS:	KNP414_01081 KNP414_07799(npr)

PMQ:	PM3016_1408 PM3016_7319

PMW:	B2K_07205 B2K_36940

PTA:	HPL003_01080 HPL003_01085 HPL003_01090

PLV:	ERIC2_c17790(npr1) ERIC2_c23330(npr2) ERIC2_c25850(npr3)

PBD:	PBOR_19115

PGM:	PGRAT_16550

PAEN:

P40081_19340

PRI:	PRIO_3659(npr)

PSON:

JI735_25370

PPEO:

ABE82_20240 ABE82_20245

PBV:	AR543_13480 AR543_13485

PXL:	BS614_28005

PKB:	B4V02_05125 B4V02_05130 B4V02_05135

PAIH:

ASL14_17665 ASL14_17675 ASL14_17680

PCHI:

PC41400_03940 PC41400_09065

PBAC:

HUB98_11520

PALR:

HGI30_05050

PKA:	PQ456_11815

PMAH:

PTQ21_30990

PAMY:

P9222_12105

PHK:	SK066_12230 SK066_12235

PNK:	AASFL403_07150

PPAB:

KET34_28385

ANX:	ACH33_08320

ATHE:

K3F53_10515

TUM:	CBW65_00785 CBW65_05655 CBW65_05660 CBW65_05665 CBW65_05670 CBW65_05675 CBW65_05680 CBW65_19395 CBW65_21440

TAB:	CIG75_01505 CIG75_01510 CIG75_08200

PLN:	Plano_1711

PRT:	AUC31_02165 AUC31_11445

PLL:	I858_000365

PDG:	BCM40_06710

PHC:	BBI08_05335

PMAR:

B0X71_00070 B0X71_15875

PPLA:

BBI15_03570 BBI15_10550

PLX:	CW734_16950

PMAT:

BBI11_08725 BBI11_16155

PDEC:

H1Q58_07885 H1Q58_15395

PGQ:	FK545_18765

SPAE:

E2C16_11120

RST:	ATY39_00320

PAEK:

D3873_00180

PANC:

E2636_18125

PLAY:

DNR44_005865 DNR44_011600

LFB:	C1X05_02975 C1X05_02980

KEB:	GXN75_14070

CAC:	CA_C2290(nprE) CA_C2517(nrpE)

CAE:	SMB_G2324 SMB_G2552(nprE)

CAY:	CEA_G2306 CEA_G2531

CBO:

CBO1446

CBA:	CLB_1466(npr-1) CLB_1467(npr-2) CLB_1468(npr-3) CLB_1470(npr-5) CLB_1471(npr-6)

CBH:	CLC_1478(npr-1) CLC_1479(npr-2) CLC_1480(npr-3) CLC_1482(npr-5) CLC_1483(npr-6)

CBY:	CLM_1605(npr) CLM_1606(npr) CLM_1607(npr) CLM_1608(npr) CLM_1609

CBL:	CLK_0923(npr) CLK_0924(npr) CLK_0925(npr) CLK_0926(npr) CLK_0927

CBB:	CLD_3104 CLD_3105(npr) CLD_3107(npr) CLD_3108(npr)

CBI:	CLJ_B1544(npr_1) CLJ_B1545(npr_2) CLJ_B1547(npr_4) CLJ_B1548(npr_5)

CBF:	CLI_1525(npr-1) CLI_1526(npr-2) CLI_1527(npr-3) CLI_1529(npr-5) CLI_1530

CBM:	CBF_1504 CBF_1508 CBF_1509

CBJ:	H04402_01511 H04402_01512 H04402_01514 H04402_01515

CLB:	Clo1100_0722

CSR:	Cspa_c37070

CLT:	CM240_2757

CSQ:

CSCA_0144

CLD:	CLSPO_c14710(nprM3) CLSPO_c14720(nprM4) CLSPO_c14730(nprM2)

CARG:

RSJ17_11665 RSJ17_11670

CDRK:

B9W14_17820

CGAS:

J1C67_18125

CRW:	CROST_031940(nprM)

CFB:	CLFE_031860(nprM)

CAUN:

CLAUR_044350(npr)

CTAG:

LL095_07975

HHW:	NCTC503_02371(npr)

OCW:	OW730_08215 OW730_18760

HSC:	HVS_05615(nprM)

RPAY:

P0092_04260

TEB:	T8CH_0602(nprE)

THYR:

P4S50_12385 P4S50_14195

MYV:	G155_14510

MVA:

Mvan_3789

MGI:

Mflv_2748

MSP:	Mspyr1_21870 Mspyr1_42680

MCB:	Mycch_3309

MFT:	XA26_30030

MVQ:

MYVA_3606

MDU:	MDUV_07800

MAUU:

NCTC10437_03546(npr)

MIJ:	MINS_33600

MTY:	MTOK_52690

MPSC:

MPSYJ_29840

MPOF:

MPOR_39650

MHOL:

K3U96_05650 K3U96_23380

NBR:	O3I_037730

NTP:	CRH09_19865

NOZ:	DMB37_16520

NAH:	F5544_13935 F5544_30865

NIE:	KV110_36910

ACIT:

HPK19_24250

AWW:	G8758_13255

RSA:	RSal33209_3168

NOI:	FCL41_09745 FCL41_16365

NDP:	E2C04_10300 E2C04_10305 E2C04_17015

NANO:

G5V58_12485

NMES:

H9L09_03060

NARO:

CFH99_13710

AER:	AERYTH_00070

KFL:

Kfla_3564

KAL:	KALB_3559 KALB_3827 KALB_8710 KALB_8711

KUT:	JJ691_16160(hly) JJ691_78810 JJ691_96900

CATI:

CS0771_76750

CCAZ:

COUCH_28280

BALA:

DSM104299_03412

NFL:	COO91_06853

HAU:

Haur_0437

ATM:

ANT_27970

ABAT:

CFX1CAM_0672

BPIT:

BPIT_27650

THYD:

TTHT_1112

SCOR:

J3U87_02535 J3U87_19455

TFO:

BFO_0703

PGIN:

FRZ67_11380 FRZ67_21095

HHY:	Halhy_3884

CHU:

CHU_1208

SRD:	SD10_22425

SMON:

AWR27_02055 AWR27_15695

SPIR:

CWM47_24010

SPIK:

EXU85_31015

SPIB:

G8759_24660 G8759_33635

STAE:

HNV11_03060

SFOL:

H3H32_34385

SENF:

GJR95_29655

RHOZ:

GXP67_06155

RUP:	DTQ70_18115

EOL:	Emtol_0024

FAE:	FAES_3027(npr)

FIB:	A6C57_25260

HSW:

Hsw_4005

HYM:	N008_09495

HYD:	PK28_00740

HYE:	AM218_15305

HYG:	AUC43_07375 AUC43_07380

HYZ:	AXW84_00730

HYH:	D3Y59_04295

HYJ:	FHG12_09725

HQI:	H9L05_08345

HRS:	HER32_08900 HER32_08905 HER32_15885

HBN:	GUY19_13055

PACT:

CA264_05485

FLM:

MY04_3789

FLL:	EI427_01095

FMM:	LVD15_09450

FUA:	LVD17_04755 LVD17_06625

GFL:

GRFL_1314

FJO:

Fjoh_0886

FBR:	FBFL15_2351

FCO:	FCOL_02025 FCOL_08450

FIN:

KQS_02660

FPAL:

HYN49_11705

FSE:	DI487_06145

CAT:	CA2559_09051

LAN:	Lacal_1859

LVN:	BWR22_10905

LACI:

CW733_01430

ASL:	Aeqsu_1324 Aeqsu_2324

AEV:	EI546_10715

MCLA:

P3875_00185

PAAE:

NPX36_00945

OLL:	CW732_03570

FEK:	C1H87_01055

KAN:	IMCC3317_26790(nprT) IMCC3317_45780(npr)

AQB:	D1818_21505

PSYN:

E9099_06530

MESQ:

C7H62_0231

FBU:	UJ101_00424(nprB) UJ101_00425

RCL:	NYR17_00355

CHZ:

CHSO_4198

CIH:	ATE47_16555

CIO:	CEQ15_09780 CEQ15_14585

CHRY:

CEY12_00875 CEY12_10630 CEY12_10635

CPIP:

CJF12_18165 CJF12_18170

CHRS:

EAG08_00900

CARH:

EGY05_17490

CNK:	EG343_09480

CJT:	EG359_10735

CCAU:

EG346_10780

CBEN:

EG339_00895

CGLE:

NCTC11432_02515(npr)

CLAC:

EG342_01180

CCAO:

H5J24_02270

CRHI:

KB553_11345

CCAB:

PFY12_06240

CHRJ:

CHRYMOREF3P_2086

CORA:

N0B40_10915 N0B40_19620

CGAM:

PFY09_04510 PFY09_08800

CJG:	NCTC13459_00235(nprM_1) NCTC13459_00589(nprM_2) NCTC13459_02696(npr)

CCAS:

EIB73_01285

CANT:

NCTC13489_01559(npr)

KFA:	Q73A0000_09480

EVA:	EIB75_02835

EZE:	KI430_10530

CNR:	EB819_01215

FLU:	CHH17_06725

OHO:	Oweho_3122

CHYD:

H4K34_07330

MAC:

MA_2075

METM:

MSMTP_2051

MEF:	MSWH1_1513

MEQ:	MSWHS_1820

MSJ:	MSSAC_2049

MSZ:	MSSIH_1718

MSW:	MSSIT_1773

» show all

Reference

1 [PMID:4967801]

Authors

Morihara K, Tsuzuki H, Oka T.

Title

Comparison of the specificities of various neutral proteinases from microorganisms.

Journal

Arch Biochem Biophys 123:572-88 (1968)
DOI:10.1016/0003-9861(68)90179-3

Reference

2 [PMID:4980359]

Authors

Millet J, Acher R.

Title

[Specificity of megateriopeptidase: an amino-endopeptidase with hydrophobic characteristics]

Journal

Eur J Biochem 9:456-62 (1969)
DOI:10.1111/j.1432-1033.1969.tb00631.x

Reference

3 [PMID:5002444]

Authors

Feder J, Keay L, Garrett LR, Cirulis N, Moseley MH, Wildi BS.

Title

Bacillus cereus neutral protease.

Journal

Biochim Biophys Acta 251:74-8 (1971)
DOI:10.1016/0005-2795(71)90061-4

Reference

4 [PMID:2276]

Authors

Holmquist B, Vallee BL.

Title

Esterase activity of zinc neutral proteases.

Journal

Biochemistry 15:101-7 (1976)
DOI:10.1021/bi00646a016

Reference

5 [PMID:6090391]

Authors

Vasantha N, Thompson LD, Rhodes C, Banner C, Nagle J, Filpula D

Title

Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein.

Journal

J Bacteriol 159:811-9 (1984)
DOI:10.1128/JB.159.3.811-819.1984

Sequence

[bao:BAMF_1546]

Reference

6 [PMID:6090407]

Authors

Yang MY, Ferrari E, Henner DJ

Title

Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation.

Journal

J Bacteriol 160:15-21 (1984)
DOI:10.1128/JB.160.1.15-21.1984

Sequence

[bsu:BSU14700]

Reference

7 [PMID:2993245]

Authors

Takagi M, Imanaka T, Aiba S

Title

Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus.

Journal

J Bacteriol 163:824-31 (1985)
DOI:10.1128/JB.163.3.824-831.1985

Sequence

[ag:AAA22621]

Reference

8 [PMID:3092843]

Authors

Sidler W, Niederer E, Suter F, Zuber H

Title

The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase.

Journal

Biol Chem Hoppe Seyler 367:643-57 (1986)
DOI:10.1515/bchm3.1986.367.2.643

Sequence

[bal:BACI_c06090]

Reference

9 [PMID:3127592]

Authors

Pauptit RA, Karlsson R, Picot D, Jenkins JA, Niklaus-Reimer AS, Jansonius JN.

Title

Crystal structure of neutral protease from Bacillus cereus refined at 3.0 A resolution and comparison with the homologous but more thermostable enzyme thermolysin.

Journal

J Mol Biol 199:525-37 (1988)
DOI:10.1016/0022-2836(88)90623-7

Sequence

[bal:BACI_c06090]

Reference

10 [PMID:2302386]

Authors

Stoeva S, Kleinschmidt T, Mesrob B, Braunitzer G.

Title

Primary structure of a zinc protease from Bacillus mesentericus strain 76.

Journal

Biochemistry 29:527-34 (1990)
DOI:10.1021/bi00454a029