Entry
Name
(R)-amidase;
R-stereospecific amidase;
R-amidase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
(R)-piperazine-2-carboxamide amidohydrolase
Reaction(IUBMB)
(1) (R)-piperazine-2-carboxamide + H2O = (R)-piperazine-2-carboxylate + NH3 [RN:
R09647 ];
(2) beta-alaninamide + H2O = beta-alanine + NH3 [RN:
R09648 ]
Reaction(KEGG)
Substrate
(R)-piperazine-2-carboxamide [CPD:
C19778 ];
H2O [CPD:
C00001 ];
beta-alaninamide [CPD:
C19779 ]
Product
Comment
In addition (R)-piperidine-3-carboxamide is hydrolysed to (R)-piperidine-3-carboxylic acid and NH3, and (R)-N-tert-butylpiperazine-2-carboxamide is hydrolysed to (R)-piperazine-2-carboxylic acid and tert-butylamine with lower activity. The enzyme does not act on the other amide substrates which are hydrolysed by EC
3.5.1.4 (amidase).
History
EC 3.5.1.100 created 2009, modified 2011
Orthology
Genes
PCQ : PcP3B5_21760(ramA_1)
PPRC : PFLCHA0_c48790(ramA)
PYM : AK972_0917 AK972_2857
BCM : Bcenmc03_1348 Bcenmc03_3529 Bcenmc03_4306
BUZ : AYM40_06155 AYM40_21415
GPN : Pan110_34850(ramA_3)
ABAC : LuPra_03237(ramA_2)
» show all
Taxonomy
Reference
Authors
Komeda H, Harada H, Washika S, Sakamoto T, Ueda M, Asano Y
Title
A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 3.5.1.100