Entry
Name
2'-N-acetylparomamine deacetylase;
btrD (gene name);
neoL (gene name);
kanN (gene name)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
2'-N-acetylparomamine hydrolase (acetate-forming)
Reaction(IUBMB)
2'-N-acetylparomamine + H2O = paromamine + acetate [RN:
R08895 ]
Reaction(KEGG)
Substrate
Product
Comment
Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. The enzyme from the bacterium Streptomyces fradiae can also accept 2'''-acetyl-6'''-hydroxyneomycin C as substrate, cf. EC
3.5.1.113 , 2'''-acetyl-6'''-hydroxyneomycin C deacetylase [2].
History
EC 3.5.1.112 created 2012
Pathway
ec00524 Neomycin, kanamycin and gentamicin biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology
K13551 2'-N-acetylparomamine deacetylase
K17078 2'-N-acetylparomamine deacetylase / 2'''-acetyl-6'''-hydroxyneomycin deacetylase
Genes
SDD : D9753_07125 D9753_17065
Taxonomy
Reference
Authors
Truman AW, Huang F, Llewellyn NM, Spencer JB
Title
Characterization of the enzyme BtrD from Bacillus circulans and revision of its functional assignment in the biosynthesis of butirosin.
Journal
Reference
Authors
Yokoyama K, Yamamoto Y, Kudo F, Eguchi T
Title
Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 3.5.1.112