KEGG   ENZYME: 3.5.1.112
Entry
EC 3.5.1.112                Enzyme                                 
Name
2'-N-acetylparomamine deacetylase;
btrD (gene name);
neoL (gene name);
kanN (gene name)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
2'-N-acetylparomamine hydrolase (acetate-forming)
Reaction(IUBMB)
2'-N-acetylparomamine + H2O = paromamine + acetate [RN:R08895]
Reaction(KEGG)
R08895
Substrate
2'-N-acetylparomamine [CPD:C17582];
H2O [CPD:C00001]
Product
paromamine [CPD:C01743];
acetate [CPD:C00033]
Comment
Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. The enzyme from the bacterium Streptomyces fradiae can also accept 2'''-acetyl-6'''-hydroxyneomycin C as substrate, cf. EC 3.5.1.113, 2'''-acetyl-6'''-hydroxyneomycin C deacetylase [2].
History
EC 3.5.1.112 created 2012
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13551  2'-N-acetylparomamine deacetylase
K17078  2'-N-acetylparomamine deacetylase / 2'''-acetyl-6'''-hydroxyneomycin deacetylase
Genes
PMUYKSS95_17150
SRNA4G23_05119(neoL)
SNFJYK04_00663(neoL)
SFBCP974_27320
STRYEQG64_18475
SDDD9753_07125 D9753_17065
SARGHKX69_16470
SPIQOHA34_16955
DVCDvina_05430
Reference
1  [PMID:17226887]
  Authors
Truman AW, Huang F, Llewellyn NM, Spencer JB
  Title
Characterization of the enzyme BtrD from Bacillus circulans and revision of its functional assignment in the biosynthesis of butirosin.
  Journal
Angew Chem Int Ed Engl 46:1462-4 (2007)
DOI:10.1002/anie.200604194
Reference
2  [PMID:18311744]
  Authors
Yokoyama K, Yamamoto Y, Kudo F, Eguchi T
  Title
Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis.
  Journal
Chembiochem 9:865-9 (2008)
DOI:10.1002/cbic.200700717
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.112
IUBMB Enzyme Nomenclature: 3.5.1.112
ExPASy - ENZYME nomenclature database: 3.5.1.112
BRENDA, the Enzyme Database: 3.5.1.112

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