KEGG ENZYME: 3.5.1.114

ENZYME: 3.5.1.114

Entry

EC 3.5.1.114                Enzyme

Name

N-acyl-aromatic-L-amino acid amidohydrolase;
aminoacylase 3;
aminoacylase III;
ACY3 (gene name)

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides

Sysname

N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)

Reaction(IUBMB)

(1) an N-acyl-aromatic-L-amino acid + H2O = an aromatic-L-amino acid + a carboxylate [RN:R10621];
(2) an N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate [RN:R10553]

Reaction(KEGG)

R10553 R10621

Substrate

N-acyl-aromatic-L-amino acid [CPD:C20735];
H2O [CPD:C00001];
N-acetyl-L-cysteine S-conjugate [CPD:C05727]

Product

aromatic-L-amino acid [CPD:C01021];
carboxylate [CPD:C00060];
L-cysteine S-conjugate [CPD:C05726];
acetate [CPD:C00033]

Comment

This enzyme is found in animals and is involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). It preferentially deacetylates Nalpha-acetylated aromatic amino acids and mercapturic acids (S-conjugates of N-acetyl-L-cysteine) that are usually not deacetylated by EC 3.5.1.14, N-acyl-aliphatic-L-amino acid amidohydrolase. The enzyme is significantly activated by Co2+ and Ni2+ [3]. Some bacterial aminoacylases demonstrate substrate specificity for both EC 3.5.1.14 and EC 3.5.1.114. cf. EC 3.5.1.14, N-acyl-aliphatic-L-amino acid amidohydrolase and EC 3.5.1.15, aspartoacylase.

History

EC 3.5.1.114 created 2013

Orthology

K18458

N-acyl-aromatic-L-amino acid amidohydrolase

Genes

HSA:	91703(ACY3)

PTR:	451373(ACY3)

PPS:	100992485(ACY3)

GGO:	101144518(ACY3)

PON:	100454588(ACY3)

NLE:	100581974(ACY3)

HMH:	116469091(ACY3)

MCC:	711083(ACY3)

MCF:	102123426(ACY3)

MTHB:

126935395

MNI:	105469695(ACY3)

CATY:

105577339(ACY3)

TGE:	112606840(ACY3)

MLEU:

105544483(ACY3)

RRO:	104664723(ACY3)

RBB:	108518683(ACY3)

TFN:	117081003(ACY3)

PTEH:

111522484(ACY3)

CANG:

105512077(ACY3)

CJC:	103796426(ACY3)

SBQ:	101028900(ACY3)

CIMI:

108310181(ACY3)

CSYR:

103249221(ACY3)

MMUR:

105867425(ACY3)

LCAT:

123642828(ACY3)

PCOQ:

105827369(ACY3)

OGA:	100965331(ACY3)

MMU:	71670(Acy3)

MCAL:

110285586(Acy3)

MPAH:

110332220(Acy3)

RNO:	293653(Acy3)

MCOC:

116100997(Acy3)

ANU:	117698058(Acy3)

MUN:	110547162(Acy3)

CGE:	100763612(Acy3)

MAUA:

101832500(Acy3)

PROB:

127228620(Acy3)

PLEU:

114694703(Acy3)

MORG:

121454502(Acy3)

MFOT:

126498283

AAMP:

119800815(Acy3)

NGI:

103725050

HGL:	101725542(Acy3)

CPOC:

100735640(Acy3)

CCAN:

109692320(Acy3)

DORD:

105989260(Acy3)

DSP:	122104969(Acy3)

PLOP:

125361329(Acy3)

NCAR:

124958758

MMMA:

107157013(Acy3)

OCU:

127486710

OPI:	101528679(ACY3)

TUP:	102503277(ACY3)

GVR:	103598001(ACY3)

CFA:	100683126(ACY3)

CLUD:

112659271(ACY3)

VLG:	121471657(ACY3)

NPO:	129503178(ACY3)

ORO:	101362499(ACY3)

EJU:	114220247(ACY3)

NSU:	110576206(ACY3)

PYU:	121023739(ACY3)

PCOO:

112851972(ACY3)

PBG:	122484384(ACY3)

PVIV:

125146594(ACY3)

PTG:	102970090(ACY3)

PPAD:

109246993(ACY3)

PUC:

125911121

AJU:

128311711

BACU:

103009750(ACY3)

BMUS:

118899618(ACY3)

LVE:	103079001(ACY3)

OOR:	101270966(ACY3)

DLE:	111184037(ACY3)

PCAD:

102979768

NASI:

112402443(ACY3)

ECB:	102149799(ACY3)

EPZ:

103544301

MYB:	102254475(ACY3) 102254790

MYD:	102764000(ACY3)

MMYO:

118665208(ACY3)

MLF:	102425715(ACY3)

PKL:	118706856(ACY3)

EFUS:

103291063(ACY3)

MNA:	107537402(ACY3)

DRO:	112317043(ACY3)

SHON:

118998407(ACY3)

AJM:	119041818(ACY3)

PDIC:

114498536(ACY3)

PHAS:

123829379(ACY3)

MMF:	118629081(ACY3)

PPAM:

129068344(ACY3)

HAI:	109384127(ACY3)

RFQ:	117029799(ACY3)

PALE:

102895489(ACY3)

PGIG:

120584744(ACY3)

PVP:	105306573(ACY3)

RAY:	107503948(ACY3)

LAV:	100668205(ACY3)

ETF:	101650595(ACY3)

DNM:	101411811(ACY3)

MDO:	100013740(ACY3)

GAS:	123251613(ACY3)

SHR:	100933605(ACY3)

AFZ:

127542126

PCW:	110195585(ACY3)

OAA:	100680665(ACY3)

ASN:	102373930(ACY3)

AMJ:	102567904(ACY3)

CPOO:

109324624(ACY3)

PSS:	102461093(ACY3)

CMY:	102932193(ACY3)

CCAY:

125639173(ACY3)

DCC:	119857656(ACY3)

CPIC:

101949865(ACY3)

TST:	117876660(ACY3)

CABI:

116833433(ACY3)

MRV:	120393383(ACY3)

ACS:	100558126(acy3)

ASAO:

132773297(ACY3)

PVT:	110085674(ACY3)

SUND:

121919516(ACY3)

PBI:	103058598(ACY3)

PMUR:

107293628(ACY3)

CTIG:

120306939(ACY3)

TSR:	106538901(ACY3)

PGUT:

117675332(ACY3)

PTEX:

113441218(ACY3)

NSS:	113410672(ACY3)

VKO:	123027525(ACY3)

PMUA:

114602141(ACY3)

PRAF:

128419366(ACY3)

ZVI:	118090290(ACY3)

HCG:	128341133(ACY3)

STOW:

125426187(ACY3)

EMC:	129324670(ACY3)

XLA:	779340(acy3.L)

XTR:	595015(acy3)

NPR:	108791080 108797385(ACY3)

RTEM:

120909172(ACY3) 120926705

BBUF:

120995479

BGAR:

122931978

MUO:	115482611(ACY3)

GSH:	117348406(ACY3)

DRE:	436619(acy3.2) 791456(acy3.1)

SRX:	107714473 107719252 107746091 107746117 107757064

SANH:

107657988 107676738 107703715 107703856

SGH:	107570819 107570825 107571911 107572870 107575636

CCAR:

109045324 109065132 109065133 109112463(acy3)

CAUA:

113044079 113044751 113062648 113084557 113084558

CGIB:

127949056 127949057 128020508

PTET:

122342326 122342355

LROH:

127159455 127165008 127165036

OMC:	131543402 131549411 131549601

PPRM:

120480155 120489148

RKG:	130082389(acy3.2)

MAMB:

125264089 125271860 125271861(acy3.2)

CIDE:

127510440 127510448 127516661

MASI:

127443795 127445050

TROS:

130552751

TDW:

130434769

MANU:

129444061(acy3.2)

IPU:	108260612 108260764

IFU:	128603980 128604518

PHYP:

113524540 113545754

SMEO:

124381101 124381703

TFD:	113641413 113650046

TVC:	132841638 132860605

AMEX:

103024465 103026039

CMAO:

118804195 118816901

EEE:	113583873 113585886

CHAR:

105902167 105912884

TRU:	101061157 101063284

TFS:	130522895 130527063

TNG:	GSTEN00011466G001 GSTEN00033497G001

LCO:	104923975 104926981

NCC:	104941420 104952637

TBEN:

117481706 117488050

CGOB:

115023841

PGEO:

117447460 117447506 117464103

GACU:

117537755 117552773

EMAC:

134864629 134876134

ELY:	117246420 117254598

EFO:	125884228 125886233

PLEP:

121942252 121961819

SLUC:

116034328 116049044

ECRA:

117934689 117955546

ESP:	116686330 116699460

PFLV:

114545402 114568528

GAT:	120822705 120825842

PPUG:

119218282 119222729

AFB:	129093177 129095633

CLUM:

117738085 117747626 117747627 117747628 117747629 117747630 117747631

PSWI:

130189671 130211905

MSAM:

119898960 119913073

SCHU:

122870550 122872968

CUD:	121505075 121508583

ALAT:

119020527 119027849

MZE:	101464677 101479803

ONL:	100691766 100698506

OAU:	116310417 116330058

OLA:

101159859

OML:	112156043 112157362(acy3.2)

CSAI:

133420069 133434752

XMA:	102227873 102230655

XCO:	114144463 114157942

XHE:	116720057 116733088

PRET:

103466257 103480428

PFOR:

103137475 103137811 103137813 103148456

PLAI:

106948229 106948230 106949604 106958436

PMEI:

106919317 106931340

GAF:	122819991 122829907(acy3.2)

PPRL:

129354913 129366906

CVG:	107091125 107100543

CTUL:

119796250 119799302

GMU:	124868723 124880988

NFU:	107375298 107377364

KMR:	108232961 108248517

ALIM:

106517035 106525346

NWH:	119408058 119409283

AOCE:

111567147 111583230

MCEP:

125004133

CSEM:

103383512

POV:	109637766 109642556

SSEN:

122767038 122770931

HHIP:

117751997 117766206

HSP:	118102323 118121144

SMAU:

118286733 118312048

LCF:	108896521 108897067

SDU:	111233002 111236611

SLAL:

111670415 111673487

XGL:	120797703 120800179

HCQ:	109511486 109520289

SSCV:

125969303

SBIA:

133505603

PEE:	133397708 133404007

PTAO:

133472969 133477223

BPEC:

110159468 110165380

MALB:

109961401 109974273

BSPL:

114857624 114865726

SJO:	128373824 128383881

SASA:

100194544(acy3) 106577455 106609950(ACY3)

STRU:

115183052 115191931 115202117

OTW:	112231784 112261060 112261397

OMY:	110497470 110531924 110534382

OGO:	124001891 124008767 124012125

ONE:	115137305 115138221 115145856

OKI:	109894170 109901108 109906771

OKE:	118362944 118369290 118395404

SALP:

111958486 111962015 112072352

SNH:	120030135 120034913 120052833

CCLU:

121550400 121554417

ELS:	105008212 105029486

SFM:	108929993 108931130

PKI:	111834701 111838492

AANG:

118227937 118231297

LOC:

102690242

PSPA:

121302115 121320145

PSEX:

120538191 120538192

LCM:	102356030 102356309

EPA:

110237552

PDAM:

113674876

SPIS:

111333775

SMIN:

v1.2.033226.t1(symbB.v1.2.033226.t1) v1.2.039538.t1(symbB.v1.2.039538.t1)

AAF:	AURANDRAFT_21222

SPAR:

SPRG_00693

EHX:	EMIHUDRAFT_252071 EMIHUDRAFT_44278 EMIHUDRAFT_44328 EMIHUDRAFT_75471

GTT:	GUITHDRAFT_118300

VAG:

N646_4536

VHR:	AL538_18035

VNI:	VIBNI_B1226

VSH:	BSZ05_19600

HJA:	BST95_01785

FRT:	F7308_1921

FRF:	LO80_06370

FMI:	F0R74_08170

GSN:	YC6258_00922

NIS:

NIS_0887

LET:	O77CONTIG1_03089

LBO:

LBWT_610

THEU:

HPC62_07415

MAR:

MAE_01890

MIQ:	B5D77_07280

MVZ:	myaer102_17080 myaer102_36990

ARP:	NIES39_O04520

LFS:	HFV01_10205

NOE:	CLI64_04765

CALT:

Cal6303_5356

CALH:

IJ00_18430

FSU:	Fisuc_0510

FSC:

FSU_0931

» show all

Reference

1 [PMID:14656720]

Authors

Pushkin A, Carpenito G, Abuladze N, Newman D, Tsuprun V, Ryazantsev S, Motemoturu S, Sassani P, Solovieva N, Dukkipati R, Kurtz I

Title

Structural characterization, tissue distribution, and functional expression of murine aminoacylase III.

Journal

Am J Physiol Cell Physiol 286:C848-56 (2004)
DOI:10.1152/ajpcell.00192.2003

Sequence

[mmu:71670]

Reference

2 [PMID:17012540]

Authors

Newman D, Abuladze N, Scholz K, Dekant W, Tsuprun V, Ryazantsev S, Bondar G, Sassani P, Kurtz I, Pushkin A

Title

Specificity of aminoacylase III-mediated deacetylation of mercapturic acids.

Journal

Drug Metab Dispos 35:43-50 (2007)
DOI:10.1124/dmd.106.012062

Sequence

[mmu:71670]

Reference

3 [PMID:19362172]

Authors

Tsirulnikov K, Abuladze N, Newman D, Ryazantsev S, Wolak T, Magilnick N, Koag MC, Kurtz I, Pushkin A

Title

Mouse aminoacylase 3: a metalloenzyme activated by cobalt and nickel.

Journal

Biochim Biophys Acta 1794:1049-57 (2009)
DOI:10.1016/j.bbapap.2009.03.022

Reference

4 [PMID:20921362]

Authors

Hsieh JM, Tsirulnikov K, Sawaya MR, Magilnick N, Abuladze N, Kurtz I, Abramson J, Pushkin A

Title

Structures of aminoacylase 3 in complex with acetylated substrates.

Journal

Proc Natl Acad Sci U S A 107:17962-7 (2010)
DOI:10.1073/pnas.1006687107

Sequence

[mmu:71670]

Reference

5 [PMID:22819785]

Authors

Tsirulnikov K, Abuladze N, Bragin A, Faull K, Cascio D, Damoiseaux R, Schibler MJ, Pushkin A

Title

Inhibition of aminoacylase 3 protects rat brain cortex neuronal cells from the toxicity of 4-hydroxy-2-nonenal mercapturate and 4-hydroxy-2-nonenal.

Journal

Toxicol Appl Pharmacol 263:303-14 (2012)
DOI:10.1016/j.taap.2012.07.002

Sequence

[rno:293653]

Other DBs

ExplorEnz - The Enzyme Database:

3.5.1.114

IUBMB Enzyme Nomenclature:

3.5.1.114

ExPASy - ENZYME nomenclature database:

3.5.1.114

BRENDA, the Enzyme Database:

3.5.1.114

DBGET integrated database retrieval system