KEGG ENZYME: 3.5.5.8

ENZYME: 3.5.5.8

Entry

EC 3.5.5.8                  Enzyme

Name

thiocyanate hydrolase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In nitriles

Sysname

thiocyanate aminohydrolase

Reaction(IUBMB)

thiocyanate + 2 H2O = carbonyl sulfide + NH3 + HO- [RN:R05780]

Reaction(KEGG)

R05780

Substrate

thiocyanate [CPD:C01755];
H2O [CPD:C00001]

Product

carbonyl sulfide [CPD:C07331];
NH3 [CPD:C00014];
HO- [CPD:C01328]

Comment

The enzyme from Thiobacillus thioparus catalyses the first step in the degradation of thiocyanate.

History

EC 3.5.5.8 created 2000

Orthology

K20760

thiocyanate hydrolase subunit alpha

K20761

thiocyanate hydrolase subunit beta

K20762

thiocyanate hydrolase subunit gamma

Genes

PDEN:

F1C79_11985 F1C79_11990(nthA)

LPO:	LPO_1236(scnC) LPO_1237 LPO_1238

LPP:	lpp1219 lpp1220 lpp1221

LLO:

LLO_1905

LSH:	CAB17_12325(scnC) CAB17_12330 CAB17_12335

LWA:	SAMEA4504053_2104(scnB) SAMEA4504053_2105(scnA) SAMEA4504053_2106(scnC)

NAX:	HC341_03860

SVA:	SVA_2685 SVA_2686 SVA_2687 SVA_2692

BGO:	BM43_3446(nthA)

BUM:	AXG89_23970 AXG89_28720

SPLB:

SFPGR_11590 SFPGR_11600 SFPGR_11610

ARUI:

G6M88_23135

RLG:

Rleg_1540

RRG:	J3P73_28990(nthA)

MPO:	Mpop_4230 Mpop_4231

MOR:	MOC_3646(nthA)

MPHY:

MCBMB27_05598

MTEZ:

HPT29_025665(nthA) HPT29_025670

RLAC:

QMO75_08300 QMO75_08305

RHZ:	RHPLAN_42300

ACUT:

MRB58_10600(nthA) MRB58_11075(nthA)

RPEL:

N7U68_16950

PAED:

G5B38_07255

PAMO:

BAR1_06170

HDH:	G5B40_13625

AMYL:

QBD29_08885(nthA) QBD29_08890

POQ:	KZX46_00445 KZX46_00450(nthA)

RMT:	IAI58_22800(scnC) IAI58_22805

SRF:	LHU95_02830

AZM:	DM194_21620(scnC) DM194_21625

HTQ:	FRZ44_32640(nthA) FRZ44_50720(nthA)

ELIO:

KO353_00995

NACI:

NUH88_01510(nthA) NUH88_01515

APB:	SAR116_1730

MIT:	OCO_27870 OCO_27880

MIA:	OCU_27740 OCU_27750

MID:	MIP_03821 MIP_03822

MYO:	OEM_26340 OEM_26350

MCHI:

AN480_14595 AN480_14600

MIR:	OCQ_26440 OCQ_26450

MMAL:

CKJ54_12660 CKJ54_12665(scnC)

MMAN:

MMAN_11070 MMAN_11080

MSA:	Mycsm_03329 Mycsm_03330

MMC:	Mmcs_5257 Mmcs_5258

MKM:	Mkms_5346 Mkms_5347

MJL:	Mjls_5636 Mjls_5637

MMM:	W7S_13500 W7S_13505

MKN:	MKAN_21490 MKAN_21495

MYV:	G155_15725 G155_15730

MYE:	AB431_16060 AB431_16065

MHAD:

B586_12050 B586_12055

MDX:	BTO20_03040 BTO20_03045

MFJ:	MFLOJ_07080 MFLOJ_07090

MSTO:

MSTO_08410 MSTO_08430

MSIM:

MSIM_52430 MSIM_52440

MLW:	MJO58_13375(scnC) MJO58_13380

MNV:	MNVI_44260 MNVI_44270

MPAG:

C0J29_16280(scnC) C0J29_16285

MHEK:

JMUB5695_03742(scnC) JMUB5695_03743

MGAU:

MGALJ_16640 MGALJ_16650

MLJ:	MLAC_17150

MSHJ:

MSHI_23270 MSHI_23280

MOT:	LTS72_18695(scnC) LTS72_18700

MMEH:

M5I08_10680 M5I08_10685(scnC)

MADI:

A7U43_06485 A7U43_06490

MSM:	MSMEG_6266 MSMEG_6267

MSG:	MSMEI_6104 MSMEI_6105

MSB:	LJ00_30985 LJ00_30990

MSN:	LI99_30990 LI99_30995

MSH:	LI98_30995 LI98_31000

MVA:	Mvan_1504 Mvan_1505

MGI:	Mflv_4915 Mflv_4916

MSP:	Mspyr1_43320 Mspyr1_43330

MCB:	Mycch_1104 Mycch_1105

MFT:	XA26_32230 XA26_32240

MPHL:

MPHLCCUG_02409(scnB) MPHLCCUG_02410(scnC)

MVQ:	MYVA_1255 MYVA_1256

MLL:	B1R94_12945

MRH:	MycrhN_4874 MycrhN_4875

MHAS:

MHAS_01849(scnB) MHAS_01850(scnC)

MDU:	MDUV_47310 MDUV_47320

MCHT:

MCHIJ_39080 MCHIJ_39090

MDR:	MDOR_37020 MDOR_37030

MAUU:

NCTC10437_01220(scnC) NCTC10437_01221(scnB)

MMOR:

MMOR_33550 MMOR_33560

MAIC:

MAIC_27730 MAIC_27740

MALV:

MALV_45510 MALV_45520

MTY:	MTOK_13040 MTOK_13050

MPSC:

MPSYJ_52350 MPSYJ_52360

MARZ:

MARA_10510 MARA_10520

MGAD:

MGAD_08190 MGAD_08200

MANY:

MANY_17080 MANY_17090

MAUB:

MAUB_50880 MAUB_50890

MPHU:

MPHO_18370 MPHO_18380

MMUC:

C1S78_009215(scnC) C1S78_009220

MMAT:

MMAGJ_31010 MMAGJ_31020

MBOK:

MBOE_50570 MBOE_50580

MFG:	K6L26_20390(scnC) K6L26_20395

MSEI:

MSEDJ_41300 MSEDJ_41310

MFLV:

NCTC10271_02504(scnC) NCTC10271_02505(scnB)

MCEE:

MCEL_07720 MCEL_07730

MMON:

EWR22_28295 EWR22_28300(scnC)

MGO:	AFA91_03540 AFA91_03545

MHOL:

K3U96_12525(scnC) K3U96_12530

MPAE:

K0O64_15105 K0O64_15110(scnC)

MRF:	MJO55_06145(scnC) MJO55_06150

MDF:	K0O62_26075(scnC) K0O62_26080

MCRO:

MI149_16100 MI149_16105(scnC)

MGRO:

FZ046_04270(scnC) FZ046_04275

MIZ:	BAB75_13855 BAB75_13860

MSTE:

MSTE_04933 MSTE_04934

MHIB:

MHIB_25770 MHIB_25780

ASD:	AS9A_3706 AS9A_3707

NFA:	NFA_20750 NFA_20760 NFA_20770

NFR:	ERS450000_02034(scnB_1) ERS450000_02035(scnA) ERS450000_02036(scnC)

NSL:	BOX37_19925 BOX37_19930

NAD:	NCTC11293_06073(scnB) NCTC11293_06074(scnC)

NTC:	KHQ06_36155 KHQ06_36160(scnC)

NGP:	LTT66_20535(scnC)

NSPU:

IFM12276_10790 IFM12276_10800 IFM12276_10810

RHA:	RHA1_ro04428 RHA1_ro04429 RHA1_ro04430

ROP:	ROP_43440(scnC) ROP_43450(scnA) ROP_43460(scnB)

ROA:	Pd630_LPD00881 Pd630_LPD00882 Pd630_LPD00883

RCR:	NCTC10994_02400(scnB_1) NCTC10994_02401(scnA) NCTC10994_02402(scnC)

RKO:	JWS14_23935(scnC) JWS14_23940 JWS14_23945

ROZ:	CBI38_03700 CBI38_03705 CBI38_03710

RPSK:

JWS13_21000 JWS13_21005 JWS13_21010(scnC)

RHOP:

D8W71_17865 D8W71_17870(scnC)

REQ:	REQ_06080 REQ_06090 REQ_06100

PDEF:

P9209_29595(scnC)

GOR:	KTR9_4744 KTR9_4745

GTA:	BCM27_25200 BCM27_25205

GRU:	GCWB2_23795(scnB) GCWB2_23800(scnC)

GAV:	C5O27_04695(scnC) C5O27_04700

GOD:	GKZ92_22695 GKZ92_22700(scnC)

GHN:	MVF96_23755 MVF96_23760(scnC)

GAMI:

IHQ52_04255(scnC) IHQ52_04260

SCYA:

EJ357_17540

SAQU:

EJC51_19945

NCX:	Nocox_22935(scnC) Nocox_22940(scnA) Nocox_22945(scnB2)

ARHD:

VSH64_07685 VSH64_07690(nthA)

PBRO:

HOP40_04470

ACTY:

OG774_01880(scnC) OG774_01885

PFLA:

Pflav_087890

DROS:

Drose_23850(scnC) Drose_23855 Drose_23860

RUB:	GBA63_05240

RMAR:

GBA65_04825

PSEU:

Pse7367_1909

» show all

Reference

1 [PMID:9573140]

Authors

Katayama Y, Matsushita Y, Kaneko M, Kondo M, Mizuno T, Nyunoya H

Title

Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase.

Journal

J Bacteriol 180:2583-9 (1998)
DOI:10.1128/JB.180.10.2583-2589.1998

Sequence

[ag:BAA28287 BAA28286 BAA28288]

Reference

2 [PMID:1577754]

Authors

Katayama Y, Narahara Y, Inoue Y, Amano F, Kanagawa T, Kuraishi H

Title

A thiocyanate hydrolase of Thiobacillus thioparus. A novel enzyme catalyzing the formation of carbonyl sulfide from thiocyanate.

Journal

J Biol Chem 267:9170-5 (1992)

Sequence

[ag:BAA28287 BAA28286 BAA28288]

Other DBs

ExplorEnz - The Enzyme Database:

3.5.5.8

IUBMB Enzyme Nomenclature:

3.5.5.8

ExPASy - ENZYME nomenclature database:

3.5.5.8

UM-BBD (Biocatalysis/Biodegradation Database):

3.5.5.8

BRENDA, the Enzyme Database:

3.5.5.8

CAS:	142539-65-9

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