KEGG ENZYME: 3.7.1.4

ENZYME: 3.7.1.4

Entry

EC 3.7.1.4                  Enzyme

Name

phloretin hydrolase;
ErPhy;
lactase-phlorerin hydrolase;
C-acylphenol hydrolase;
2',4,4',6'-tetrahydroxydehydrochalcone 1,3,5-trihydroxybenzenehydrolase (incorrect)

Class

Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances

Sysname

phloretin acylhydrolase (phloroglucinol-forming)

Reaction(IUBMB)

phloretin + H2O = phloretate + phloroglucinol [RN:R02901]

Reaction(KEGG)

R02901;
(other) R13082

Substrate

phloretin [CPD:C00774];
H2O [CPD:C00001]

Product

phloretate [CPD:C01744];
phloroglucinol [CPD:C02183]

Comment

Also hydrolyses other C-acylated phenols related to phloretin. Isolated from the fungus Aspergillus niger and the bacteria Pantoea agglomerans and Eubacterium ramulus.

History

EC 3.7.1.4 created 1972, modified 2018

Pathway

ec00946

Degradation of flavonoids

ec01100

Metabolic pathways

Orthology

K22906

phloretin hydrolase

Genes

RGE:	RGE_01480 RGE_12780

TAK:	Tharo_0198

LPG:	BB562_02250

CLJ:	CLJU_c30240

CLS:	CXIVA_00870 CXIVA_01400 CXIVA_03650 CXIVA_10480 CXIVA_16390 CXIVA_20190

CSR:	Cspa_c22710

CAH:	CAETHG_1031

CSQ:

CSCA_1095

CBUT:

ATN24_18505

CRW:	CROST_033500(phy_1)

CFB:	CLFE_033220(phy)

CAUN:

CLAUR_043040(phy)

CTAG:

LL095_01940

CALE:

FDN13_06055

FPLA:

A4U99_15225

CARL:

PXC00_03325

ALKA:

J0B03_05305

LXA:	OW255_13800

LBX:

lbkm_0103

ETM:	CE91St48_18380 CE91St48_29990

AFN:	Acfer_0965

» show all

Reference

1 [PMID:5354935]

Authors

Chatterjee AK, Gibbins LN

Title

Metabolism of phloridzin by Erwinia herbicola: nature of the degradation products, and the purification and properties of phloretin hydrolase.

Journal

J Bacteriol 100:594-600 (1969)
DOI:10.1128/JB.100.2.594-600.1969

Reference

2 [PMID:5441377]

Authors

Minamikawa T, Jayasankar NP, Bohm BA, Taylor IE, Towers GH.

Title

An inducible hydrolase from Aspergillus niger, acting on carbon-carbon bonds, for phlorrhizin and other C-acylated phenols.

Journal

Biochem J 116:889-97 (1970)
DOI:10.1042/bj1160889

Reference

3 [PMID:15466559]

Authors

Schoefer L, Braune A, Blaut M

Title

Cloning and expression of a phloretin hydrolase gene from Eubacterium ramulus and characterization of the recombinant enzyme.

Journal

Appl Environ Microbiol 70:6131-7 (2004)
DOI:10.1128/AEM.70.10.6131-6137.2004

Sequence

[ag:AAQ12341]

Other DBs

ExplorEnz - The Enzyme Database:

3.7.1.4

IUBMB Enzyme Nomenclature:

3.7.1.4

ExPASy - ENZYME nomenclature database:

3.7.1.4

BRENDA, the Enzyme Database:

3.7.1.4

CAS:	37289-38-6

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