KEGG   ENZYME: 4.1.99.14
Entry
EC 4.1.99.14                Enzyme                                 
Name
spore photoproduct lyase;
SAM;
SP lyase;
SPL;
SplB;
SplG
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
Sysname
spore photoproduct pyrimidine-lyase
Reaction(IUBMB)
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA) = thymidylyl-(3'->5')-thymidylate (in double-helical DNA)
Substrate
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA)
Product
thymidylyl-(3'->5')-thymidylate (in double-helical DNA)
Comment
This enzyme is a member of the 'AdoMet radical' (radical SAM) family. The enzyme binds a [4Fe-4S] cluster. The cluster is coordinated by 3 cysteines and an exchangeable SAM molecule [3]. The 5'-deoxy-adenosine radical formed after electron transfer from the [4Fe-4S] cluster to the S-adenosyl-L-methionine, initiates the repair by abstracting the C-6 hydrogen of the spore photoproduct lesion. During the second part of the repair process the SAM molecule is regenerated [3].
History
EC 4.1.99.14 created 2009, modified 2010
Orthology
K03716  spore photoproduct lyase
Genes
MEAMex_1p2913
MDIMETDI3483
MEXMext_2717
MCHMchl_2944
MPOMpop_2839
MZAB2G69_21125
MRDMrad2831_0830
METM446_5726
MORMOC_1042
METAY590_13060
MAQUMaq22A_c04270(splB)
MPHYMCBMB27_00427
MEEDA075_14255
METDC0214_15240
METXA3862_26405
METSDK389_08850
METIDK427_05815
MMESMMSR116_21955
MTEADK419_06950
MINDmvi_44200
MTADM6G65_03455
MTEZHPT29_006080
AUAM673_19365
JAVOXU80_11450
STAXMC45_09865
SPLKAV944_09820
SPAUDRN02_007415
SARIH5J25_10105
SAERNEF64_04235
SMORLHA26_18430
SGLCM1K48_07995
SRADLLW23_03680
SNAPPQ455_13460
SSAGKV697_19580
SPHSETR14_21275
POQKZX46_13145
PMEAKTC28_11200
LCKHN018_07950
BSUBSU13930(splB)
BSRI33_1571(splB)
BSLA7A1_1539
BSHBSU6051_13930(splB)
BSYI653_07160
BSUTBSUB_01518(splB)
BSULBSUA_01518(splB)
BSUSQ433_07995
BSOBSNT_07885(splB)
BSNBSn5_19030
BSQB657_13930(splB)
BSXC663_1436(spIB)
BSPU712_07320
BSSBSUW23_07160(splB)
BSTGYO_1727(splB)
BITBIS30_17770
BLIBL03571(splB)
BLDBLi01602(splB)
BLHBaLi_c16380(splB)
BAYRBAM_013700(splB)
BAQBACAU_1344(splB)
BYABANAU_1306(splB)
BAMPB938_07140
BQYMUS_1476(splB)
BAMLBAM5036_1309(splB)
BAMARBAU_1353(splB)
BAMNBASU_1332(splB)
BAMBBAPNAU_2389(splB)
BAMTAJ82_07880
BAMYV529_13270(spIB)
BMPNG74_01427(splB)
BAOBAMF_1470(splB)
BAZBAMTA208_10180(splB)
BQLLL3_01497(splB)
BXHBAXH7_02077(spIB)
BAMIKSO_012520
BAMCU471_13990
BAMFU722_07305
BSIACWD84_14400(splB)
BAEBATR1942_04730
BVMB9C48_07015
BSONS101395_03351(splB)
BHTDIC78_02460(splB)
BMOJHC660_14590(splB)
BTEQG4P54_07305(splB)
BIQAN935_07275
BSTRQI003_07550(splB)
BANBA_4429(splB)
BARGBAA_4429(splB)
BATBAS4109
BAHBAMEG_4463(splB)
BAIBAA_4445(splB)
BAXH9401_4222
BANTA16_44240
BANRA16R_60260
BANSBAPAT_4247
BANHHYU01_21615
BANVDJ46_3114(splB)
BCEBC4205
BCABCE_4278(splB)
BCZBCE33L3959(splB)
BCRBCAH187_A4334(splB)
BCBBCB4264_A2489 BCB4264_A4315(splB)
BCUBCAH820_4225(splB)
BCGBCG9842_B0919(splB)
BCQBCQ_3992(splB)
BCXBCA_4313(splB)
BALBACI_c41720(splB)
BNCBCN_4118
BCFbcf_20900
BCERBCK_14160
BCEFBcrFT9_03303
BTKBT9727_3948(splB)
BTLBALH_3810(splB)
BTBBMB171_C3863(splB)
BTTHD73_3502
BTHRYBT1520_17270
BTHIBTK_17260
BTCCT43_CH4219(splB)
BTFYBT020_20755
BTMMC28_3498
BTGBTB_c43470(splG)
BTIBTG_07420 BTG_28370
BTNBTF1_10640 BTF1_19425
BTHTH175_ch4287
BTHUYBT1518_23230
BTWBF38_5374(splB)
BTHYAQ980_13750
BWEBcerKBAB4_4058
BWWbwei_0732(splB)
BMYOBG05_1812(splB)
BTYBtoyo_1450
BMYCDJ92_5435(splB)
BBYCY96_11380 CY96_29050
BWDCT694_22640(splB)
BTROFJR70_14805(splB)
BMOBMLA2C4_21995(splB)
BNTGSN03_20000(splB)
BPACLMD38_05955(splB)
BPANNLJ82_20455(splB)
BALUQRY64_23945(splB)
BPUBPUM_1283
BPUMBW16_07225
BPUSUP12_06720
BIFN288_17950
BGYBGLY_1595(splB)
BWHA9C19_12555
BXIBK049_01640
BALTCFN77_07085(splB)
BAERBAE_14305(splB)
BSAFBSL056_06685
BFDNCTC4823_03632(splB)
BSHILGQ02_05655(splB)
BCABEFK13_07760(splB)
BRYM0696_07630(splB)
BJSMY9_1527
BACIB1NLA3E_15610
GSTHW35_13900
BACWQR42_06580
BACPSB24_02845
BACBOY17_09945
BACOOXB_0887
BACYQF06_05995
BACLBS34A_15490(splB)
BALMBsLM_1482
BACSAUL54_03690
BACQDOE78_16985(splB)
BACAFAY30_12555(splB)
BAQUK6959_08215(splB)
BZHNF868_05945(splB)
BHAIKJK41_06855(splB)
BCAOLC087_02935(splB)
BARDQRY57_16920(splB)
BAEIRE735_12955(splB)
BCOBcell_1416
BHKB4U37_07535
BCOHBC6307_08905
BCIRC2I06_21420(splB)
NTXNQZ71_08585(splB)
BMQBMQ_2226(splB)
BMDBMD_2184(splB)
BMHBMWSH_3013(splB)
BMEGBG04_4595(splB)
BEOBEH_06890
PKSIE339_04750(splB)
BHABH2808(splB)
BKWBkAM31D_16990(splB)
BCLABC2488(splB)
BLEBleG1_2467
SHUAPQ477_00370(splB)
BGIBGM20_01330
BPFBpOF4_01635(splB)
BONA361_20295
BKOCKF48_19300(splB)
CGOTJ1899_10240(splB)
CSUAIM538_16660(splB)
CFIRNAF01_18390(splB)
CSOALIS82_16745(splB)
CSPGLS684_14285(splB)
CPSSM5V91_00635(splB)
BDAFSZ17_20070(splB)
OIHOB2284
GTMGT3921_07390
BCALCWI35_16330(splB)
GTNGTNG_2348
GYCGYMC61_0226
GYAGYMC52_2440
GCTGC56T3_1073
GGHGHH_c24920(splB)
GSEGT50_14820
GEJA0V43_09975
GZAIC807_08235(splB)
PCALBV455_03004(splG)
PARGPspKH34_11140(splB)
AFLAflv_0922(splB)
AGNAFK25_09950
ANMGFC28_3756(splB)
AAMYGFC30_2607(splB)
ANLGFC29_1677(splB)
ANDGRQ40_12825(splB)
ACAIISX45_10160(splB)
ASEDIRT44_12890(splB)
LSPBsph_2057
LGYT479_08045
LFUHR49_16195
LYSLBYS11_07245(splB)
LYBC3943_08975(splB)
LYZDCE79_16115(splB)
LYGC1N55_20145(splB)
LPAKGDS87_07835(splB)
LMACI6G82_10680(splB)
LYCFH508_0008340(splB)
LCAPICJ70_16990(splB)
LYPMTP04_39000(splB)
LIUOU989_07155(splB)
HHDHBHAL_5162
HMNHM131_01240
HLIHLI_12055(splB)
HNZP9989_04710(splB)
HSANMUN89_02000(splB)
HSHIMUO14_11380(splB)
HAMYMUO15_10610(splB)
TAPGZ22_01400
TAIDKS242_02560(splB)
VIRX953_08050
VHLBME96_06965
VIGBKP57_18595
VNECFK40_16350(splB)
VPNA21D_03910(splB)
VPTKBP50_11115(splB)
FPNABE65_013900
FARABE41_012655
FECQNH15_13915(splB)
ACOPRI196_06595(splB)
BSJUP17_16190
BMURABE28_015045
PBUTDTO10_03100(splB)
PASABAOM_2138(splB)
PPSRI6J18_20810(splB)
PFRIL8956_16920(splB)
AQTFN924_01915(splB)
RAXKO561_01765(splB)
BTHVCQJ30_08680(splB)
PSYHD0S48_12620(splB)
PSYOPB01_06175(splB)
PSYBKD050_07700(splB)
PRDF7984_13385(splB)
GCSMUN88_18620(splB)
GSMMUN87_00410(splB)
POFGS400_13245(splB)
PCHUQNI29_14270(splB)
NMKCHR53_12655(splB)
NDTL1999_21400(splB)
NCMQNK12_10705(splB)
NNVQNH39_13055(splB)
MEKUHUW50_18330(splB)
MSEMGMB29_06630(splB)
MDGK8L98_09420(splB)
MLITKDJ21_022730(splB)
AIAAWH56_026195(splB)
BLENNCTC4824_04058(splB)
SEDDERJ70_01030(splB)
RMFD5E69_07950(splB)
RAZU9J35_07345(splB)
BVJI5776_02680(splB)
BOUI5818_02865(splB)
MCUIG8O30_02735(splB)
GAJMY490_07725(splB)
SLMSMM221_16635(splB)
MJOFOF60_16940(splB)
MSUTLC048_05520(splB)
FHLOE105_05340(splB)
FAFOE104_05595(splB)
ECTOMUG87_07635(splB)
ALKLMM271_10030(splB)
PHJLC071_19550(splB)
STEAC0679_03665(splB)
BBEBBR47_21270(splB)
BLRBRLA_c014700
BFMBP422_05850
BAGRBA6348_18450(splB)
BRWGOP56_00280(splB)
BCHSJNE38_11270(splB)
BCOPJD108_13865(splB)
BPABPSE45_10490(splB)
BHUILOK74_11590(splB)
BAYDBSPP4475_12630(splB)
BBORRFB14_16240(splB)
PJDPjdr2_3157
GYMGYMC10_0367
PPYPPE_02867
PPMPPSC2_15205(splB)
PPOPPM_3056(splB)
PPOLX809_31595
PPQPPSQR21_030230(splB)
PPOYRE92_21515
PMSKNP414_02639(splG)
PMQPM3016_2883
PMWB2K_14645
PTAHPL003_24190
PLVERIC2_c33130(splG)
PSABPSAB_16765
PBDPBOR_24675
PGMPGRAT_21900
PODPODO_21685
PAENP40081_24700
PAEFR50345_21680
PAEQR50912_22890
PAEAR70723_22405
PAEER70331_21490
PAEHH70357_25115
PAEJH70737_22040
PBJVN24_25365
PIHUB51_04795
PRIPRIO_4700(splB)
PPEOABE82_15615
PNPIJ22_25150
PXLBS614_23190
PSWUSY83_19355
PIBBBD41_14595
PKBB4V02_09925
PVOPVOR_04078
PLWD5F53_27675(splB)
PLENEIM92_15030(splB)
PPSCEHS13_20685(splB)
PLUTEI981_01500(splB)
PALBEJC50_13810(splB)
PCHIPC41400_11025(splB)
PBACHUB98_06935(splB)
PRZGZH47_05215(splB)
PLYCGXP70_11080(splB)
PSOPKP014_23160(splB)
PTRIKDC22_21810(splB)
PALOE6C60_1728
PCELHUB94_15910(splB)
PTJJRJ22_20500(splB)
PALRHGI30_09540(splB)
PTHINDS46_18575(splB)
PUIPUW25_17490(splB)
PMAHPTQ21_26165(splB)
PAMYP9222_16835(splB)
PMAELMZ02_24085(splB)
PWNQNH46_13765(splB)
PSPNL1F29_10815(splB)
PPOGQPK24_16855(splB)
PAUNMJA45_17480(splB)
PPABKET34_23005(splB)
COHEAV92_09905(splB)
COHNKCTCHS21_24540(splB)
CCHLFPL14_25975(splB)
CHEBHH215_03125(splB)
ACURJZ785_18250(splB)
BTSBtus_0889
KYRCVV65_01810(splB)
EFFskT53_14990(splB)
SIVSSIL_0558
SSILSOLI23_15965
SPSYAZE41_00895
SPOSDV702_13290(splB)
SPAEE2C16_13560(splB)
SPOOJ3U78_14875(splB)
SURLBI350_01330
STHOQNH10_14100(splB)
RSTATY39_04390
PANCE2636_17850(splB)
LFBC1X05_16055(splB)
TVUAB849_005480(splB)
KEBGXN75_15935(splB)
CACCA_C0699
CAESMB_G0713(splB)
CAYCEA_G0710
CPECPE0724
CPFCPF_0720(splB)
CTCCTC_00900
CTETBN906_00948
CNONT01CX_1399
CBOCBO0216(splB)
CBACLB_0257(splB)
CBHCLC_0272(splB)
CBYCLM_0266(splB)
CBLCLK_3398(splB)
CBKCLL_A1824(splB)
CBBCLD_0559(splB)
CBICLJ_B0264(splB)
CBNCbC4_5006
CBTCLH_1683(splB)
CBFCLI_0281(splB)
CBMCBF_0249
CBJH04402_00205
CBECbei_4361
CBZCbs_4361
CBEILF65_04885
CKLCKL_3385
CKRCKR_2992
CLJCLJU_c33020
CLBClo1100_1688
CSRCspa_c46410(splB)
CAHCAETHG_1200(splB)
CBVU729_346(splB)
CSQCSCA_1344
CLDCLSPO_c02490(splB)
CACECACET_c37310(splB)
CCKCcar_07560
CBUTATN24_10405
CEUA7L45_11760
CTAEBGI42_07730(splB)
CFMBJL90_04365
CCHVBTM20_11390
CARGRSJ17_02925
CDRKB9W14_12105
CSEPCP523_04015(splB)
CDYF3K33_22305(splB)
CGASJ1C67_18240(splB)
CLOSDMR38_16520(splB)
CRWCROST_011660(splB)
CFBCLFE_036710(splB)
CAUNCLAUR_017210(splB)
CGELpsyc5s11_32260(splB)
CTAGLL095_13840(splB)
CINTHZF06_05120(splB)
CALEFDN13_13525(splB)
CPRFK7H06_16130(splB)
SARJHH195_05165(splB)
OCWOW730_22880(splB)
CCECcel_0624
RPAYP0092_09860(splB)
CSSCst_c25500(splG)
CSDClst_2438
CCELCCDG5_1063(splB)
EHAEthha_2128
RGVNQ502_00035
OVAOBV_20430
TMELNOG13_07440
PETRQKW49_05435(splB)
PALMRBG61_02825
CAPREQM14_02890
CFEMHCR03_16140
AGATRWV98_07260
SWOSwol_1838
SALQSYNTR_1224
DSYDSY2469
DHDDhaf_3628
DDLDesdi_2528
DMTDESME_08640
SGYSgly_1116
DORDesor_1498
DAIDesaci_2222
DMIDesmer_1505
DEDDHBDCA_p1682 DHBDCA_p343
DECDCF50_p1691 DCF50_p404
DRSDEHRE_10515 DEHRE_12195
DCADesca_2324
PTHPTH_0212(SplB) PTH_2267(SplB)
DRMDred_0691
DRUDesru_3329
DFGB0537_03890
DAEDtox_0238
DKUDesku_0981
DAUDaud_0667
TFRBR63_01385(splB)
FWADCMF_10425
TJRTherJR_2202
DGIDesgi_0200 Desgi_0265
HMOHM1_2342(spIB)
HCVFTV88_2150(splB)
ACAEHYG86_02555(splB)
BYLA4V09_09440
BPROPMF13cell1_03437(splB)
BLARLC508_06965
BCOCBLCOC_24960(splB)
CPYCphy_1777
LACYA4V08_25510
CHYLCE91St63_23430
CSHClosa_2552
LXAOW255_13125
ARFAR1Y2_1723
ACACEYQ97_08175
ANRAna3638_05660
ACELacsn021_44890
ACHTbsdcttw_49110
CBOLCGC65_06015
CASPNQ535_07750
KIBRBB56_17245
LBXlbkm_0173
CPROCPRO_16080(splB)
CLEClole_3036
CEWEKH84_18125(splB)
VGUHYG85_10480(splB) HYG85_13450
VPYHZI73_01900(splB)
ABUTAmi103574_10330
ETMCE91St48_33240
AMTAmet_0662
AOEClos_0743
GFEGferi_09125
PBIFKXZ80_08475(splB)
PSORRSJ16_05775
CDFCD630_02750(splB)
PDCCDIF630_00399(splB)
CDCCD196_0292(spl)
CDLCDR20291_0279(spl)
PDFCD630DERM_02750(splB)
TEMJW646_05000(splB)
TGCL0P85_03385(splB)
TMYTEMA_38600(splB)
TEBT8CH_2443(splB)
THYRP4S50_04955(splB)
IBRNQ514_04305(splB)
TPZTph_c28660(splB)
GEKkuro4_15790
TTOThethe_01995
TXYThexy_1006
MTAMoth_1928
MTHOMOTHE_c19660(splB)
MTHZMOTHA_c20450(splB)
BACGD2962_14495(splB)
TOCToce_1736
NTHNther_2341
NCDACONDI_02675(splB)
HORHore_22270
HALSD7D81_01230(splB)
IFNGM661_17300(splB)
AARAcear_1290
HHLHalha_1370
KMEH0A61_01600(splB)
CADCuri_c06870(splB)
TISP3962_09675(splB)
TIZRBU61_09995(splB)
SGBIP3F81_10320
PUFUFO1_0963
PFTJBW_03484
STEDSPTER_08420(splG)
TURAT726_08580
TSGHLK68_08265(splB)
TBBJ0J69_11775(splB)
CALKHUE98_15465(splB)
MKUI2456_01075
MPAGC0J29_14280
MWUPT015_00325
MMAGMMAD_38100(splB)
MARZMARA_42050
MSEIMSEDJ_31420
MRFMJO55_15750
MGROFZ046_07620
RHBNY08_4313
RFAA3L23_02432(splG)
RHSA3Q41_00934(splG)
RHUA3Q40_03923(splG)
WHROG579_18175
ACITHPK19_01535(splB)
AAGINCTC2676_1_01220(splG)
KFLKfla_4483
GOBGobs_4985
MMARMODMU_5497
KRAKrad_4460
PBROHOP40_00930
ACTYOG774_17640
MSAGGCM10017556_58060(splB)
MHAWRMN56_23350
AMSAMIS_30680
ACTNL083_3843
AFSAFR_15210
CCAZCOUCH_20445
CMPCha6605_5221
CYNCyan7425_3293
CEPCri9333_0169
LENLEP3755_37460
LBOLBWT_7990
KOVK9N68_38360
TSINOXH18_09910
GLPGlo7428_4765
CHONNIES4102_30820
GDUP0S91_04690
CYJCyan7822_5934
MICMic7113_5958
NPUNpun_F6062
NONNOS3756_38760
NFLCOO91_01733
NEDHUN01_06175
HBQQI031_12030
CALNNIES2098_30840
RIVRiv7116_4324
TOQHCG51_01145
NCNBZZ01_14145
CTHEChro_1073
SCSSta7437_1049
STANSTA3757_00520
TRATrad_0283
PHMPSMK_31830
ACMAciX9_4343
PMUOLOK61_14530
MROBHH214_16895
SLISlin_1066
SRDSD10_18045
SMONAWR27_14585
SPIRCWM47_29980
SPIKEXU85_00555
STAEHNV11_17020
SRHOHH216_10440
SOQLQ777_17145
FAEFAES_3815
FIBA6C57_17910
HYGAUC43_11630
HYPA0257_20320
HYZAXW84_14660
HNVDDQ68_09050
HYHD3Y59_06965
HYJFHG12_01305
HQIH9L05_00295
HTSHMJ29_14345
HMTMTP16_21650
HTBMTX78_08025
HSBMWH26_05450
HAEIMUN82_03610
HCUMUN79_15285
HVLMUN86_07400
HCFMUN80_14180
PKOPKOR_20505
RTIDC20_00240
ADDHUW48_16730
ASWUHUW51_22390
MBAKMSBR3_0651
 » show all
Reference
1  [PMID:16829676]
  Authors
Chandor A, Berteau O, Douki T, Gasparutto D, Sanakis Y, Ollagnier-de-Choudens S, Atta M, Fontecave M
  Title
Dinucleotide spore photoproduct, a minimal substrate of the DNA repair spore photoproduct lyase enzyme from Bacillus subtilis.
  Journal
J Biol Chem 281:26922-31 (2006)
DOI:10.1074/jbc.M602297200
  Sequence
[bsu:BSU13930]
Reference
2  [PMID:16968710]
  Authors
Pieck JC, Hennecke U, Pierik AJ, Friedel MG, Carell T
  Title
Characterization of a new thermophilic spore photoproduct lyase from Geobacillus  stearothermophilus (SplG) with defined lesion containing DNA substrates.
  Journal
J Biol Chem 281:36317-26 (2006)
DOI:10.1074/jbc.M607053200
Reference
3  [PMID:16829680]
  Authors
Buis JM, Cheek J, Kalliri E, Broderick JB
  Title
Characterization of an active spore photoproduct lyase, a DNA repair enzyme in the radical S-adenosylmethionine superfamily.
  Journal
J Biol Chem 281:25994-6003 (2006)
DOI:10.1074/jbc.M603931200
  Sequence
[bsu:BSU13930]
Reference
4  [PMID:19012397]
  Authors
Mantel C, Chandor A, Gasparutto D, Douki T, Atta M, Fontecave M, Bayle PA, Mouesca JM, Bardet M
  Title
Combined NMR and DFT studies for the absolute configuration elucidation of the spore photoproduct, a UV-induced DNA lesion.
  Journal
J Am Chem Soc 130:16978-84 (2008)
DOI:10.1021/ja805032r
Reference
5  [PMID:20405152]
  Authors
Silver SC, Chandra T, Zilinskas E, Ghose S, Broderick WE, Broderick JB
  Title
Complete stereospecific repair of a synthetic dinucleotide spore photoproduct by  spore photoproduct lyase.
  Journal
J Biol Inorg Chem 15:943-55 (2010)
DOI:10.1007/s00775-010-0656-8
  Sequence
[cac:CA_C0699]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.14
IUBMB Enzyme Nomenclature: 4.1.99.14
ExPASy - ENZYME nomenclature database: 4.1.99.14
BRENDA, the Enzyme Database: 4.1.99.14
CAS: 37290-70-3

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