KEGG   ENZYME: 4.2.1.74
Entry
EC 4.2.1.74                 Enzyme                                 
Name
medium-chain-enoyl-CoA hydratase;
long-chain enoyl coenzyme A hydratase (incorrect);
long-chain-enoyl-CoA hydratase (incorrect);
long-chain-(3S)-3-hydroxyacyl-CoA hydro-lyase (incorrect)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
medium-chain-(3S)-3-hydroxyacyl-CoA hydro-lyase
Reaction(IUBMB)
a medium-chain (3S)-3-hydroxyacyl-CoA = a medium-chain trans-2-enoyl-CoA + H2O [RN:R02685]
Reaction(KEGG)
Substrate
medium-chain (3S)-3-hydroxyacyl-CoA
Product
medium-chain trans-2-enoyl-CoA;
H2O [CPD:C00001]
Comment
Acts in the reverse direction. The best substrate for the porcine enzyme is oct-2-enoyl-CoA. Unlike EC 4.2.1.17 enoyl-CoA hydratase, it does not act on crotonoyl-CoA.
History
EC 4.2.1.74 created 1981, modified 2022
Pathway
ec00062  Fatty acid elongation
ec00071  Fatty acid degradation
ec01100  Metabolic pathways
Reference
1  [PMID:833142]
  Authors
Fong JC, Schulz H.
  Title
Purification and properties of pig heart crotonase and the presence of short chain and long chain enoyl coenzyme A hydratases in pig and guinea pig tissues.
  Journal
J Biol Chem 252:542-7 (1977)
Reference
2  [PMID:4828315]
  Authors
Schulz H.
  Title
Long chain enoyl coenzyme A hydratase from pig heart.
  Journal
J Biol Chem 249:2704-9 (1974)
Reference
3  [PMID:20463021]
  Authors
Arent S, Christensen CE, Pye VE, Norgaard A, Henriksen A
  Title
The multifunctional protein in peroxisomal beta-oxidation: structure and substrate specificity of the Arabidopsis thaliana protein MFP2.
  Journal
J Biol Chem 285:24066-77 (2010)
DOI:10.1074/jbc.M110.106005
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.74
IUBMB Enzyme Nomenclature: 4.2.1.74
ExPASy - ENZYME nomenclature database: 4.2.1.74
BRENDA, the Enzyme Database: 4.2.1.74
CAS: 62009-81-8

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