KEGG ENZYME: 4.2.2.19

ENZYME: 4.2.2.19

Entry

EC 4.2.2.19                 Enzyme

Name

chondroitin B lyase;
chondroitinase B;
ChonB;
ChnB

Class

Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides

Sysname

chondroitin B lyase

Reaction(IUBMB)

Eliminative cleavage of dermatan sulfate containing (1->4)-beta-D-hexosaminyl and (1->3)-beta-D-glucurosonyl or (1->3)-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups to yield a 4,5-unsaturated dermatan-sulfate disaccharide (DeltaUA-GalNAc-4S).

Reaction(KEGG)

(other) R10871

Comment

This is the only lyase that is known to be specific for dermatan sulfate as substrate. The minimum substrate length required for catalysis is a tetrasaccharide [2]. In general, chondroitin sulfate (CS) and dermatan sulfate (DS) chains comprise a linkage region, a chain cap and a repeat region. The repeat region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(beta1-3)GalNAc(beta1-]n, which may be O-sulfated on the C-4 and/or C-6 of GalNAc and C-2 of GlcA. GlcA residues of CS may be epimerized to iduronic acid (IdoA) forming the repeating disaccharide [-4)IdoA(alpha1-3)GalNAc(beta1-]n of DS. Both the concentrations and locations of sulfate-ester substituents vary with glucosaminoglycan source [5].

History

EC 4.2.2.19 created 2005

Orthology

K19053

chondroitin B lyase

Genes

BND:	KWG56_16495

PHE:

Phep_0789

PSN:	Pedsa_3807

MDJ:	LLH06_06740

ASAG:

FGM00_07255

CSAL:

NBC122_01604(cslB)

Reference

1 [PMID:8526872]

Authors

Gu K, Linhardt RJ, Laliberte M, Gu K, Zimmermann J.

Title

Purification, characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum.

Journal

Biochem J 312 ( Pt 2):569-77 (1995)
DOI:10.1042/bj3120569

Reference

2 [PMID:12063249]

Authors

Pojasek K, Raman R, Kiley P, Venkataraman G, Sasisekharan R.

Title

Biochemical characterization of the chondroitinase B active site.

Journal

J Biol Chem 277:31179-86 (2002)
DOI:10.1074/jbc.M201552200

Sequence

[phe:Phep_0789]

Reference

3 [PMID:11500043]

Authors

Pojasek K, Shriver Z, Kiley P, Venkataraman G, Sasisekharan R.

Title

Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum.

Journal

Biochem Biophys Res Commun 286:343-51 (2001)
DOI:10.1006/bbrc.2001.5380

Reference

4 [PMID:12443044]

Authors

Suzuki K, Terasaki Y, Uyeda M.

Title

Inhibition of hyaluronidases and chondroitinases by fatty acids.

Journal

J Enzyme Inhib Med Chem 17:183-6 (2002)
DOI:10.1080/14756360290032930

Reference

5 [PMID:427837]

Authors

Ototani N, Yosizawa Z.

Title

Purification of chondroitinase B and chondroitinase C using glycosaminoglycan-bound AH-Sepharose 4B.

Journal

Carbohydr Res 70:295-306 (1979)
DOI:10.1016/S0008-6215(00)87109-8

Reference

6 [PMID:10618199]

Authors

Tkalec AL, Fink D, Blain F, Zhang-Sun G, Laliberte M, Bennett DC, Gu K, Zimmermann JJ, Su H

Title

Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum.

Journal

Appl Environ Microbiol 66:29-35 (2000)
DOI:10.1128/AEM.66.1.29-35.2000

Sequence

[phe:Phep_0789]

Reference

7 [PMID:15155751]

Authors

Michel G, Pojasek K, Li Y, Sulea T, Linhardt RJ, Raman R, Prabhakar V, Sasisekharan R, Cygler M.

Title

The structure of chondroitin B lyase complexed with glycosaminoglycan oligosaccharides unravels a calcium-dependent catalytic machinery.

Journal

J Biol Chem 279:32882-96 (2004)
DOI:10.1074/jbc.M403421200

Reference

8 [PMID:10216304]

Authors

Li Y, Matte A, Su H, Cygler M.

Title

Crystallization and preliminary X-ray analysis of chondroitinase B from Flavobacterium heparinum.

Journal

Acta Crystallogr D Biol Crystallogr 55:1055-7 (1999)
DOI:10.1107/S0907444999002097

Reference

9 [PMID:10600383]

Authors

Huang W, Matte A, Li Y, Kim YS, Linhardt RJ, Su H, Cygler M.

Title

Crystal structure of chondroitinase B from Flavobacterium heparinum and its complex with a disaccharide product at 1.7 A resolution.

Journal

J Mol Biol 294:1257-69 (1999)
DOI:10.1006/jmbi.1999.3292

Sequence

[phe:Phep_0789]

Reference

10 [PMID:16336265]

Authors

Huckerby TN, Nieduszynski IA, Giannopoulos M, Weeks SD, Sadler IH, Lauder RM.

Title

Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides.

Journal

FEBS J 272:6276-86 (2005)
DOI:10.1111/j.1742-4658.2005.05009.x

Other DBs

ExplorEnz - The Enzyme Database:

4.2.2.19

IUBMB Enzyme Nomenclature:

4.2.2.19

ExPASy - ENZYME nomenclature database:

4.2.2.19

BRENDA, the Enzyme Database:

4.2.2.19

CAS:	52227-83-5

DBGET integrated database retrieval system