KEGG ENZYME: 4.2.2.22

ENZYME: 4.2.2.22

Entry

EC 4.2.2.22                 Enzyme

Name

pectate trisaccharide-lyase;
exopectate-lyase;
pectate lyase A;
PelA

Class

Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides

Sysname

(1->4)-alpha-D-galacturonan reducing-end-trisaccharide-lyase

Reaction(IUBMB)

eliminative cleavage of unsaturated trigalacturonate as the major product from the reducing end of polygalacturonic acid/pectate

Comment

Differs in specificity from EC 4.2.2.9, pectate disaccharide-lyase, as the predominant action is removal of a trisaccharide rather than a disaccharide from the reducing end. Disaccharides and tetrasaccharides may also be removed [2].

History

EC 4.2.2.22 created 2007

Reference

1 [PMID:12443532]

Authors

Kluskens LD, van Alebeek GJ, Voragen AG, de Vos WM, van der Oost J

Title

Molecular and biochemical characterization of the thermoactive family 1 pectate lyase from the hyperthermophilic bacterium Thermotoga maritima.

Journal

Biochem J 370:651-9 (2003)
DOI:10.1042/BJ20021595

Sequence

[tma:TM0433]

Reference

2 [PMID:11259664]

Authors

Tamaru Y, Doi RH.

Title

Pectate lyase A, an enzymatic subunit of the Clostridium cellulovorans cellulosome.

Journal

Proc Natl Acad Sci U S A 98:4125-9 (2001)
DOI:10.1073/pnas.071045598

Reference

3 [PMID:14673544]

Authors

Berensmeier S, Singh SA, Meens J, Buchholz K.

Title

Cloning of the pelA gene from Bacillus licheniformis 14A and biochemical characterization of recombinant, thermostable, high-alkaline pectate lyase.

Journal

Appl Microbiol Biotechnol 64:560-7 (2004)
DOI:10.1007/s00253-003-1446-9

Sequence

[bld:BLi04129]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.2.22

IUBMB Enzyme Nomenclature:

4.2.2.22

ExPASy - ENZYME nomenclature database:

4.2.2.22

BRENDA, the Enzyme Database:

4.2.2.22

All links

Protein sequence (125)   
   UniProt (111)   
   SWISS-PROT (5)   
   RefSeq(pep) (8)   
   PDBSTR (1)   
DNA sequence (150)   
   RefSeq(nuc) (45)   
   GenBank (52)   
   EMBL (53)   
3D Structure (1)   
   PDB (1)   
Protein domain (4)   
   InterPro (4)   
All databases (280)   

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