KEGG ENZYME: 4.2.3.16

ENZYME: 4.2.3.16

Entry

EC 4.2.3.16                 Enzyme

Name

(4S)-limonene synthase;
(-)-(4S)-limonene synthase;
4S-(-)-limonene synthase;
geranyldiphosphate diphosphate lyase (limonene forming);
geranyldiphosphate diphosphate lyase [cyclizing, (4S)-limonene-forming];
geranyl-diphosphate diphosphate-lyase [cyclizing;
(-)-(4S)-limonene-forming]

Class

Lyases;
Carbon-oxygen lyases;
Acting on phosphates

Sysname

geranyl-diphosphate diphosphate-lyase [cyclizing; (S)-limonene-forming]

Reaction(IUBMB)

geranyl diphosphate = (S)-limonene + diphosphate [RN:R02013]

Reaction(KEGG)

R02013

Substrate

geranyl diphosphate [CPD:C00341]

Product

(S)-limonene [CPD:C00521];
diphosphate [CPD:C00013]

Comment

A recombinant enzyme (also known as a monoterpene synthase or cyclase) from the grand fir (Abies grandis) requires Mn2+ and K+ for activity. Mg2+ is essentially ineffective as the divalent metal ion cofactor.

History

EC 4.2.3.16 created 2000 as EC 4.1.99.10, transferred 2000 to EC 4.2.3.16, modified 2003

Pathway

ec00902

Monoterpenoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K15088

(4S)-limonene synthase

K21925

(-)-camphene/tricyclene/(4S)-limonene/myrcene synthase

K21926

(+)-alpha-terpineol/(4S)-limonene synthase

Genes

EGR:

104440568

RCU:

8276874

SLY:	101245212(TPS3) 101249864

SPEN:

107012939 107012948

SOT:	102591915 102602070

SSTN:

125861389 125861571

CANN:

107840093

LBB:

132598839

NTA:	107785945 107787835

NSY:

104238653

NTO:

104090536

NAU:

109214608

SSPL:

121755010

SMIL:

131005775 131026618 131026619

SHIS:

125185612 125185636 125193586 125197284 125200997 125204274 125208619 125218604 125219856 125220542 125224358

CSIN:

114273830 114302023

» show all

Reference

1 [PMID:9268308]

Authors

Bohlmann J, Steele CL, Croteau R

Title

Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.

Journal

J Biol Chem 272:21784-92 (1997)
DOI:10.1074/jbc.272.35.21784

Sequence

[ag:AAB70907]

Reference

2 [PMID:8226816]

Authors

Colby SM, Alonso WR, Katahira EJ, McGarvey DJ, Croteau R

Title

4S-limonene synthase from the oil glands of spearmint (Mentha spicata). cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase.

Journal

J Biol Chem 268:23016-24 (1993)

Sequence

[ag:AAC37366]

Reference

3 [PMID:8806736]

Authors

Yuba A, Yazaki K, Tabata M, Honda G, Croteau R

Title

cDNA cloning, characterization, and functional expression of 4S-(-)-limonene synthase from Perilla frutescens.

Journal

Arch Biochem Biophys 332:280-7 (1996)
DOI:10.1006/abbi.1996.0343