KEGG   ENZYME: 4.2.3.87
Entry
EC 4.2.3.87                 Enzyme                                 
Name
alpha-guaiene synthase;
PatTps177 (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
(2Z,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, alpha-guaiene-forming)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate = alpha-guaiene + diphosphate [RN:R09887]
Reaction(KEGG)
R09887
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448]
Product
alpha-guaiene [CPD:C20161];
diphosphate [CPD:C00013]
Comment
Requires Mg2+. The enzyme from Pogostemon cablin gives 13% alpha-guaiene as well as 37% (-)-patchoulol (see EC 4.2.3.70), 13% delta-guaiene (see EC 4.2.3.93), and traces of at least ten other sesquiterpenoids [1]. In Aquilaria crassna three clones of the enzyme gave about 80% delta-guaiene and 20% alpha-guaiene, with traces of alpha-humulene. A fourth clone gave 54% delta-guaiene and 45% alpha-guaiene [2].
History
EC 4.2.3.87 created 2011
Orthology
K15807  patchoulol/alpha-guaiene/delta-guaiene synthase
K22036  alpha/delta-guaiene synthase
Reference
1  [PMID:16970904]
  Authors
Deguerry F, Pastore L, Wu S, Clark A, Chappell J, Schalk M
  Title
The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases.
  Journal
Arch Biochem Biophys 454:123-36 (2006)
DOI:10.1016/j.abb.2006.08.006
  Sequence
[ag:AAS86323]
Reference
2  [PMID:20959422]
  Authors
Kumeta Y, Ito M
  Title
Characterization of delta-guaiene synthases from cultured cells of Aquilaria, responsible for the formation of the sesquiterpenes in agarwood.
  Journal
Plant Physiol 154:1998-2007 (2010)
DOI:10.1104/pp.110.161828
  Sequence
[ag:ACY38195 ACY38196 ACY38197]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.87
IUBMB Enzyme Nomenclature: 4.2.3.87
ExPASy - ENZYME nomenclature database: 4.2.3.87
BRENDA, the Enzyme Database: 4.2.3.87

  All links  
Chemical substance (3)   
   KEGG COMPOUND (3)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (6)   
   KEGG ORTHOLOGY (2)   
   KEGG GENES (4)   
Protein sequence (8)   
   UniProt (4)   
   SWISS-PROT (4)   
Protein domain (7)   
   InterPro (7)   
All databases (26)   

Download RDF
DBGET integrated database retrieval system