KEGG ENZYME: 4.3.1.28

ENZYME: 4.3.1.28

Entry

EC 4.3.1.28                 Enzyme

Name

L-lysine cyclodeaminase;
rapL (gene name);
fkbL (gene name);
tubZ (gene name);
visC (gene name)

Class

Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases

Sysname

L-lysine ammonia-lyase (cyclizing; ammonia-forming)

Reaction(IUBMB)

L-lysine = L-pipecolate + NH3 [RN:R10277]

Reaction(KEGG)

R10277

Substrate

L-lysine [CPD:C00047]

Product

L-pipecolate [CPD:C00408];
NH3 [CPD:C00014]

Comment

Requires bound NAD+. The enzyme produces the non-proteinogenic amino acid L-pipecolate, which is incorporated into multiple secondary metabolite products, including rapamycin, tobulysin, virginiamycin and pristinamycin.

History

EC 4.3.1.28 created 2012

Orthology

K20500

L-lysine cyclodeaminase

Genes

MFB:	MFUL124B02_23075 MFUL124B02_27410

AVM:	JQX13_51800

MBOK:

MBOE_03980

SRC:	M271_40660

SMAL:

SMALA_7577 SMALA_8315

SSOI:

I1A49_41665 I1A49_45315

STRM:

M444_32730

SPRI:

SPRI_0308 SPRI_7045

SGS:	AVL59_38715

SQZ:	FQU76_32205

SCYA:

EJ357_00955

SKA:	CP970_02210

SPAD:

DVK44_35860

STSU:

B7R87_01735

SXN:	IAG42_36265

SDD:	D9753_01960

SGK:	PET44_31170

SCOA:

QU709_19870

KQI:	F1D05_02760

SSYI:

EKG83_25915

MCAB:

HXZ27_12710

ACTN:

L083_2969(rapL)

AFS:

AFR_18570

» show all

Reference

1 [PMID:9473033]

Authors

Khaw LE, Bohm GA, Metcalfe S, Staunton J, Leadlay PF

Title

Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase.

Journal

J Bacteriol 180:809-14 (1998)
DOI:10.1128/JB.180.4.809-814.1998

Sequence

[ag:CAA60467]

Reference

2 [PMID:16536560]

Authors

Gatto GJ Jr, Boyne MT 2nd, Kelleher NL, Walsh CT

Title

Biosynthesis of pipecolic acid by RapL, a lysine cyclodeaminase encoded in the rapamycin gene cluster.

Journal

J Am Chem Soc 128:3838-47 (2006)
DOI:10.1021/ja0587603

Sequence

[ag:CAA60467]

Reference

3 [PMID:17291665]

Authors

Tsotsou GE, Barbirato F

Title

Biochemical characterisation of recombinant Streptomyces pristinaespiralis L-lysine cyclodeaminase.

Journal

Biochimie 89:591-604 (2007)
DOI:10.1016/j.biochi.2006.12.008

Other DBs

ExplorEnz - The Enzyme Database:

4.3.1.28

IUBMB Enzyme Nomenclature:

4.3.1.28

ExPASy - ENZYME nomenclature database:

4.3.1.28

BRENDA, the Enzyme Database:

4.3.1.28

All links

Chemical substance (3)   
   KEGG COMPOUND (3)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (29)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (28)   
Protein sequence (269)   
   UniProt (268)   
   SWISS-PROT (1)   
DNA sequence (242)   
   GenBank (121)   
   EMBL (121)   
Protein domain (3)   
   InterPro (3)   
All databases (548)   

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